• Title/Summary/Keyword: Classification of Basidiomycetes

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Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea

  • Nam, Jin-Sik
    • Korean Journal of Environmental Biology
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    • v.23 no.4
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    • pp.335-342
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    • 2005
  • We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

Taxonomic Studies on the Genus Lactarius of Korea(I) (한국산(韓國産) 무당버섯과(科)의 분류학적(分類學的) 연구(硏究)(제1보)(第1報) -Lactarius속(屬)의 분류(分類)-)

  • Bok, Jin-Deok;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.13 no.4
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    • pp.249-262
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    • 1985
  • Mushrooms were collected at Mt. Gyeryong, Mt. Chilgab, Mt. Ducyu, Mt. Chiri, Mt Songri and the regions of Chungnam Province for 15 months from July, 1983 to October, 1984. They were classified into 19 species, four varieties and one form of Lactarius. These species were grouped into six sections according to Hesler and Smith. Of these Lactarius, nine species and four varieties appeared to be newly found ones.

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