• 제목/요약/키워드: Classification of Basidiomycetes

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Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea

  • Nam, Jin-Sik
    • 환경생물
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    • 제23권4호
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    • pp.335-342
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    • 2005
  • We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

한국산(韓國産) 무당버섯과(科)의 분류학적(分類學的) 연구(硏究)(제1보)(第1報) -Lactarius속(屬)의 분류(分類)- (Taxonomic Studies on the Genus Lactarius of Korea(I))

  • 복진덕;신관철
    • 한국균학회지
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    • 제13권4호
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    • pp.249-262
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    • 1985
  • 1983년(年) 7월(月)부터 84년(年) 10월(月)까지 지리산(地異山), 속리산(俗離山), 덕유산(德裕山), 칠갑산(七甲山), 계룡산(鷄龍山) 지역(地域) 및 충남일원(忠南一圓)에서 젖버섯 속(屬)에 속하는 균류(菌類)들을 채집(探集)하여 분류(分類)한 결과(結果) Lactarius 19종, 4변종 및 1 form이 동정(同定)되었다. 이들 Lactarius를 Hesler 및 Smith(1979)의 체계(體係)에 따라 6 section으로 나누었으며 이중 한국(韓國)에서 처음 발견된 버섯은 다음과 같다. L. lignyotus var. nigroviolascens (Atk.) Hesler & Smith 보라잿빛 헛대 젖버섯, L. sumstinei Pk. 우산주름 젖버섯, L. subvellereus var. subdistans Hesler & Smith 노란주름융단 젖버섯, L. vellereus var. virescens Hesler & Smith 푸른주름융단 젖버섯, L. piperatus var. glaucescens (Crossl.) Hesler & Smith 녹색젖 굴털이, L. luteolus Pk. 갈색끈적 젖버섯, L. pyrogalus (Fr.) Fr. 제브라 젖버섯, L. argillaceifolius var. argillaceifolius Hesler & Smith 흑갈색주름 젖버섯, L. repraesentaneus Britz. 보라빛주름 젖버섯, L. mutabilis Pk. 진갈색무늬 젖버섯, L. thejogalus (Fr.) S.F. Gray 갈황색 젖버섯, L. gracilis Hongo 애기 털 젖버섯, L. cyathula f. japonicus Hongo 애기낙엽 젖버섯.

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