• Journal of Mushroom
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• v.4 no.2
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• pp.48-52
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• 2006

Oyster mushroom, Pleurotus ostreatus is bifactorial heterothallism. Single basidiospore isolates from fruiting bodies are homokaryotic and self-sterile. However, we found that homokaryons derived from some strains of P. ostreatus could develop fruiting bodies with two different types. One hundred and two isolates out of 155 monospore isolates formed fruiting bodies (65.8%). First group did not have only mature or sporulating fruiting bodies but also clamp connections, which initial isolate also did not present clamp connections (Abortive homokaryotic fruiting, AHF). Second group had developed fruiting bodies with clamp connections even though initial homokaryotic colony did not form clamp connections (Pseudo- homokaryotic fruiting, PHF). The mycelial colonies derived from PHF by tissue culture formed clamp connections, while mycelial colonies of AHF lacked them. We obtained 535 PHF and 79 AHF inter-strain hybrids among 8 strains of P. ostreatus by hyphal anastomosis. The fruiting body yield of PHF group is higher than that of AHF group in bottle cultivation. A preselection of single spore isolates for fertility would save labour in strain improvement of P. ostreatus.

• Smart Structures and Systems
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• v.13 no.4
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• pp.567-585
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• 2014

The piezoelectric transverse $d_{15}$ shear sensing mechanism is firstly assessed experimentally for a cantilever smart sandwich plate made of a piezoceramic axially poled patched core and glass fiber reinforced polymer composite faces. Different electrical connections are tested for the assessment of the sensor performance under a varying amplitude harmonic (at 24 Hz) force. Also, the dynamic response of the smart sandwich composite structure is monitored using different acquisition devices. The obtained experimentally sensed voltages are compared to those resulting from the benchmark three-dimensional piezoelectric coupled finite element simulations using a commercial code where realistic features, like equipotential conditions on the patches' electrodes and mechanical updating of the clamp, are considered. Numerically, it is found that the stiffness of the clamp, which is much softer than the ideal one, has an enormous influence on the sensed voltage of its adjacent patch; therefore, sensing with the patch on the free side would be more advantageous for a cantilever configuration. Apart from confirming the latter result, the plate benchmark experimental assessment showed that the parallel connection of its two oppositely poled patches has a moderate performance but better than the clamp side patch acting as an individual sensor.

• Magazine of the Korean Society of Agricultural Engineers
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• v.43 no.6
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• pp.113-119
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• 2001

Experiments on the yield strength of pipe connectors made of metal wire, joint pins, pole pipes, multi span insertion joints, and T-clamp joints used in pipe houses were conducted. The strength of connections of a pipe connector made of metal wire was adequate but it had a big difference according to loading direction. Therefore as it is installed, its direction should be taken into consideration. The collapse load of pipes connected with a joint pin was lower than that of single pipes. In the part of frame member at which the great bending moment occurs, the use of joint pin should be avoided. Also experimental results showed that pole pipes for use in a part of frame buried under the ground were safe, and the strength of multi span insertion joints should be increased. The resistant moment of T-clamp was about 13.7% of a single pipe. In case that the external forces acting on left and right rafter are different. a unsymmetrical rotational force is produced at the multi span joint. If it is expected that the actual bending moment on the multi span joint is larger than resistant moment of T-clamp, a reinforcement to safely resist the rotational force is required.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2001.10a
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• pp.271-274
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• 2001

Experiments on the yield strength of pipe connectors made of metal wire, joint pins, pole pipes, multi span insertion joints, and T-clamp joints used in pipe houses were conducted. The strength of connections of a pipe connector made of metal wire was adequate but it had a big difference according to loading direction. The collapse load of pipes connected with a joint pin was lower than that of single pipes. Also experimental results showed that pole pipes for use in a part of frame buried under the ground were safe, and the strength of multi span insertion joints should be increased. The resistant moment of T-clamp was about 13.7% of a single pipe.

• Journal of Mushroom
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• v.7 no.4
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• pp.141-149
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• 2009

The sexual incompatibility system was investigated in the new strain Pleurotus ostreatus 'Miso'. Matings among monosporous progeny collected from fruiting bodies revealed that the new oyster mushroom is heterothallic and has a tetrapolar mating system. Single basidiospore isolates that did not have clamp connections were determined to be monokaryotic. Compatible parings of monokaryons were distinguishable macroscopically by the rapid growth and gross morphology to some extent but they were distinguished obviously by the presence of clamp connections from incompatible pairings under microscopic examination. Rarely dikaryotization was unidirectional with clamp connections only at the margin of one side of mating. In tetrapolarity of matings, four kinds of patterns, A1B1, A1B2, A2B1, and A2B2, were decided depending on compatibility tests in a parental strain with a mating factor of A1A2B1B2. The mating tests with the tester strains were performed for the detection of mating types on the rest of selected monokaryons.

• Mycobiology
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• v.37 no.4
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• pp.305-307
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• 2009

An unrecorded Ceriporiopsis species was collected at Mt. Gariwang, Gangwon Province, in 2008. Based on morphological characteristics, such as a fully resupinate basidiocarp, a reddish white to pinkish poroid hymenophore and a monomitic hyphal system with clamp connections, the species was identified as Ceriporiopsis resinascens. This is the first report of Ceriporiopsis resinascens in Korea. We confirmed the identity of the species as Ceriporiopsis resinascens based on ITS sequence analysis.

• The Korean Journal of Mycology
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• v.15 no.3
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• pp.135-141
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• 1987

Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopiae protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.321-326
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• 1998

Two strains, each of Tremella fuciformis and Hypoxylon sp. were isolated and their mycelial properties were investigated. T. fuciformis produced yeast-like conidia and dikaryotic mycelia from white to yellow color. The diameter of the hyphae was $1.5\;to\;3.0\;{\mu}m$, and septa with clamp connections were present. Secondary mycelia with clamp connections could produce fruitbodies on sawdust medium when the environmental conditions were suitable for fructification. The symbiotic fungus, Hypoxylon sp. produced white feather-like mycelia. But the color of old mycelia was changed to light yellow or light brown and pigmented the culture medium from light brown to brown or very dark green. Generally it did not produce conidia, but in a special case it produced conidia of which color was yellow green to grass green and of which shape was sub-elliptical with the size of approximately $3\;to\;5\;{\mu}m$.

• Research in Plant Disease
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• v.16 no.3
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• pp.323-325
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• 2010

The stem rot of sunflower (Helianthus annuus) occurred sporadically in the experimental field of Gyeongsangnam-do Agricultural Research and Extension Services, on September, 2009. The infected plants were wilted and water-soaked brown spots were formed on the stem, than infected stems were mostly died. White mycelial mats were spread over lesions, and then sclerotia were formed on stem and near soil line. The sclerotia were globoid in shape, 1~3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on PDA was $30^{\circ}C$ and the hyphal width was $4{\sim}8\;{\mu}m$. The typical clamp connections were observed in the hyphae of the pathogenic fungus. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii. This is the first report on the stem rot of sunflower by S. rolfsii in Korea.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.203-205
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• 2008

From 2006 to 2008, the stem rot of Petunia hybrida Vilm. caused by Sclerotium rolfsii occurred sporadically at Jinju city in Gyeongsangnam-do. The typical symptom is water-soaking, brown on the stem and rotting, wilting and blighting. The infected plants were mostly died. White mycelial mats were spread over lesions, and then sclerotia were formed on stem and near soil line. The sclerotia were globoid in shape, $1{\sim}3\;mm$ in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation was at $30^{\circ}C$ on PDA and the hyphal width ranged from 4 to $8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus. On the basis of mycological characteristics and pathogenicity to P. hybrida, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of P. hybrida caused by S. rolfsii in Korea.