• Title/Summary/Keyword: Citrus Species

Search Result 123, Processing Time 0.027 seconds

Effects of Ground Vegetation and Pyrethroid Spray on the Population Dynamics of Panonychus citri (Acari: Tetranychidae) and Natural Enemies in Citrus Orchard: A Short-term Effect (감귤원에서 초생관리와 합성피레스로이드계 조합처리가 귤응애와 천적의 발생양상에 미치는 단기효과)

  • Hyun, Seung Young;Kim, Dong-Soon
    • Korean journal of applied entomology
    • /
    • v.61 no.1
    • /
    • pp.255-266
    • /
    • 2022
  • This study was conducted to examine the effects of grass vegetation (W: manual weeding, NW: herbicide sprays) and pyrethroid spray (P: pyrethroid spray, NP: no pyrethroid spray) on the population dynamics of Panonychus citri and natural enemies in citrus orchards. Two essential hypothesis were made to test the population dynamics: 1) weed planting promotes natural enemies by offering habitat and alternative food sources, resulting in the reduction of P. citri populations, and 2) pyrethroid spray removes natural enemies by its non-selective toxicity, resulting in the increasement of P. citri populations. The observed natural enemy populations (mainly Phytoseiids and Agistemus sp.) were not different largely from the expected values in the hypothesis, which assumes more abundant natural enemies in weeds and no pyrethroid plots. Although some discrepancy was occurred in NW+NP and W+NP plots in 2011, the observed values were almost same with expected values in 2012. In overall, pesticide effect was strongly significant and pyrthroids removed largely natural enemies. Although habitat (weeds) effect showed a conflict result, natural enemy population increased in plots allowing weed growth, when considering the increased autumn population relatively compared to that of spring-summer population. The decreased abnormal P. citri populations in pyrethroid plots could be explained under the assumption of a strong repellent behavior of P. citri to the pyrethroids.

Effects of Arbuscular Mycorrhizae on Growth and Mineral Nutrient Contents in Trifoliate Orange Seedling (Arbuscular Mycorrhizae가 탱자 유묘의 생육과 무기양분 함량에 미치는 영향)

  • Oh, Hyun-Woo;Kim, Sang-Youb;Han, Hae-Ryong;Moon, Doo-Khil;Chung, Jong-Bae
    • Korean Journal of Environmental Agriculture
    • /
    • v.17 no.2
    • /
    • pp.182-188
    • /
    • 1998
  • The effect of arbuscular mycorrhizae(AM) on the growth of trifoliate orange seeding were investigated in volcanic ash soil. Trifoliate orange is used as a root stock of citrus in Cheju island. Seedings innoculated with AM fungi were grown for 16 weeks in pots of various levels of fertilizer. Growth characteristics and mineral nutrient contents were measured and spores of AM fungi colonized were identified. Seventy % of the replicsted pots of seedings were colonized by AM in the treatment of high level fertilizer and additional phosphate (40g of 21-17-17 complex fertilizer and 50g of fused phosphate added to 50L of soil). In treatments of low levels fertilizer or without fused phosphate addition, the pots colonized were less than 20 %. Colonization of trifoliate seedings with AM fungi greatly increased the growth of seedings. Shoot length and weight of shoot and root positively regressed on AM colonization ratio. AM colonization caused higher concentrations of P, Cu and Mg in plant, and the relations were significant at 5 % level. Contents of N and Zn in plants also tended to increase, while that of Ca to decrease, with increasing colonization ratio. Four species of AM fungi - Glomus deserticola, G. rubiforme, G. vesiculiferum and Acaulospora sp, - were found in the soil where roots of trifoliate orange as an innoculation materials were collected. All of the 4 species were found in the inoculated pot soils after the seedling growth, indicating that these species can be colonized in trifoliate orange roots.

  • PDF

Plant Species Utilization and Care Patterns Using Potted Plants in the Traditional Gardening (전통조경에서 분(盆)을 이용한 식물의 활용과 애호 행태)

  • Kim, Myung-Hee
    • Journal of the Korean Institute of Traditional Landscape Architecture
    • /
    • v.31 no.3
    • /
    • pp.61-74
    • /
    • 2013
  • This study examined and analyzed ancient writing and poetry regarding cases of appreciating plants by using pots in a garden of a palace or private houses by ancestors, and examined shape and planting method of plant species and potted plants, arrangement and preference of potted plants. As for the method of the study, description research method which examines and interprets poem and painting based on potted plants. The results of this study is summarized like the following. First, the plants which were favorably used for potted plants include 19 kinds such as Prunus mume, Pinus densiflora, Pinus pumila, Phyllostachys spp., Camellia japonica, Punica granatum, and Gardenia jasminoidesa, and as for herbs, 12 kinds such as Chrysanthemum monifolium and Nelumbo nusifera . Second, the species which were specially arranged into artificial shapes include Prunus mume and Pinus densiflora. The two plants made the shape of severe curves of stems such as Wangpi. Gyuban, and Bangan, and there are Pinus densiflora dwarfed potted plant whose roots are stretched on Prunus mume grafted into a strange stump and an oddly shaped stone. For the beauty of the dwarfed tree shape, pine cones are added to an old Pinus densiflora or Parthenocissus tricuspidata is planted to stems, and additional method of making moss on the soil, which is called 'Jongbunchuigyeong'. As for planting method, water culture, planting on a stone, planting on a charcoal, and assembled planting are expressed in poetry. Third, as for external space for potted plants, a place where a king stays, a bed room for a king, surrounding areas and gardens of private houses, and step stones were used as a space which adds artistic effects. Potted plants are placed on a table in a library, on a desk, on a drawer, and near a pillow as a small items in a room, and scholars enjoyed original characteristics and symbolism of the potted plants. Fourth, at the time of flowering of Prunus mume, poetry event was held to enjoy the tree and writing poetry begun. And at the time of flowering of Chrysanthemum monifolium, the flowers were floated in a liquor glass or shadow play was enjoyed. Fifth, potted plants played the role of garden ornaments in elegant events of a palace, the gentry, wedding ceremony, and sacrificial rites. Sixth, potted plants were used as tributes between countries, donation to a king, or a gift of a king. In addition, there were many cases where scholars exchanged potted plants and there is the first record of giving a potted plant in 'Mokeunsigo' by Mokeun Isaek, scholar in the late era of Goryeo. Seventh, at the time of flowering Prunus mume, Chrysanthemum monifolium, Gardenia jasminoides, Nelumbo nusifera, and Narcissustazetta var. chinensis, they enjoyed the particular fragrance and express it into poetry. Eighth, plant species from southern parts such as Camellia japonica, Daphne odora, Gardenia jasminoides, Citrus unshiu, Phyllostachys spp., Punica granatum, Rosa rugosa, and Musa basjoo, or foreign plant species, and species weak against the cold were utilized as pot plants for enjoying green trees indoors in northern central province in harshly cold winter.

Base Study for Improvement of School Environmental Education with the Education Indigenous Plants - In the case of Mapo-Gu Elementary School in Seoul - (자생식물 교육을 통한 학교 환경교육 개선에 관한 기초연구 - 서울시 마포구 초등학교를 중심으로 -)

  • Bang, Kwang-Ja;Park, Sung-Eun;Kang, Hyun-Kung;Ju, Jin-Hee
    • Journal of the Korean Society of Environmental Restoration Technology
    • /
    • v.3 no.1
    • /
    • pp.10-19
    • /
    • 2000
  • Due to the urbanization, concentrated population, and limited land exploitation in the modern society, the environment surrounding that we live in is getting polluted more and more, and it has become hard even to let urban children experience the nature. This research was conducted to help people recognize the importance of our natural resources through the environmental education of elementary school and to use school's practical open-space for the Indigenous Plants education. The results of this study are as follows : First, the status of a plant utilization in our institutional education : There were 362 species totally of 124 species of Trees, 156 species of Herbs, 63 species of Crops, and 19 species of Hydrophytes which appear in the elementary school text book. Of all, the most frequently appearing species of tree were the Malus pumila var. dulcissima, Pinus densijlora, Citrus unshiu, Diospyros kaki. Second, the effect of plant education using the land around schools : The result of research on the open-space of the 19 elementary schools located in Mapo-gu showed that most of the species planted are the Juniperus chinensisrose, Hibiscus syriacus. Pelargonium inquinans in the order of size, and the plants appearing in text book were grown in the botanical garden organized in 7 schools. Especially most of the Indigenous Plants were being planted in botanical garden, and Pinus densijlora, Abeliophyllum distichum, Polygonatum var. plurijlorum, Liriope platyphylla and so on. Last, the result of this research on recognition of Environment, Planting education and Indigenous plants : It showed that educational necessity of students and teachers about environment and Indigenous Plants was more than 80%. The management of botanical garden was conducted by some teachers and managers. The results of this study suggested that we needed the reconstruction of curriculum, the efficient application of plant education for effectiveness of using school environment and monitoring continually and construction information sources for the better environment education in the elementary schools.

  • PDF

Chemical Composition and Antiproliferative Activity of Supercritical Extract of Immature Citrus Peel in human cervical carcinoma HeLa cells (미성숙 감귤 과피 초임계 추출물의 성분 분석과 자궁암세포 성장억제효능)

  • Moon, Jeong Yong;Song, YeonWoo;Hyun, Ho Bong;KimCho, Somi
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.16 no.12
    • /
    • pp.8836-8843
    • /
    • 2015
  • This study was performed to investigate the antiproliferative activities of supercritical extracts from phalsak(Citrus hassaku Hort ex Tanaka) and yeagam(Citrus iyo Hort. ex Tanaka) against human cervical carcinoma HeLa cells and the chemical compositions of the extracts. The anticancer properties of supercritical extracts were demonstrated using the MTT assay and Hoechst 33342 staining and the compositional analyses were conducted by using gas chromatography-mass spectrometry(GC-MS). The peel extracts of both species exhibited similar antiproliferative effect. The antiproliferative activity of the flesh extracts was not detected up to $400{\mu}g/mL$, whereas peel extracts of phalsak and yeagam reduced cell viability with 87.16% and 92.95% at $400{\mu}g/mL$, respectively. There was a dramatic increase of the apoptotic body formation in the cell treated with peel extracts while no apoptotic body formation detected in the cell treated with flesh extracts at 100, $200{\mu}g/mL$. By GC-MS analysis, 27 and 31 kinds of compounds identified in flesh and peel of phalsak, while 27 and 29 kinds of compounds were identified in flesh and peel of yeagam, respectively. 1,1,4,4-Tetramethyl-2-tetralone(20.86%), alloimperatorin(8.15%), limonene(11.23%), and auraptene(7.29%) were major in peel of phalsak, whereas limonene(22.19%), linalool(11.23%), and ${\gamma}$-sitosterol(9.12%) were major in peel of yeagam.

Molecular Characterization of a Transient Expression Gene Encoding for 1-Aminocyclopropane-1-carboxylate Synthase in Cotton (Gossypium hirsutum L.)

  • Wang, Xia;Zhang, Ying;Zhang, Jiedao;Cheng, Cheng;Guo, Xingqi
    • BMB Reports
    • /
    • v.40 no.5
    • /
    • pp.791-800
    • /
    • 2007
  • Ethylene performs an important function in plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS), the key enzyme involved in ethylene biosynthesis, has been the focus of most ethylene studies. Here, a cotton ACS gene referred to as Gossypium hirsutum ACS1 (GhACS1), was isolated. The full-length cDNA of GhACS1 encodes for a 476-amino acid protein which harbors seven conserved regions, 11 invariant amino acid residues, and the PLP binding active site, all of which characterize ACC synthases. Alignment analysis showed that GhACS1 shared a high degree of identity with other known ACC synthases from different species. Two introns were detected in the genomic DNA sequence, and the results of Southern blot analysis suggested that there might be a multi-gene family encoding for ACC synthase in cotton. From the phylogenetic tree constructed with 24 different kinds of ACC synthases, we determined that GhACS1 falls into group II, and was closely associated with the wound-inducible ACS of citrus. The analysis of the 5' flanking region of GhACS1 revealed a group of putative cis-acting elements. The results of expression analysis showed that GhACS1 displayed its transient expression nature after wounding, abscisic acid (ABA), and $CuCl_2$ treatments. These results indicate that GhACS1, which was transiently expressed in response to certain stimuli, may be involved in the production of ethylene for the transmission of stress signals.

Fungicidal Activities of 51 Fruit-Derived Extracts in vivo against Six Phytopathogenic Fungi

  • Lee, Hoi-Seon;Lee, Seon-Woo;Cho, Kwang-Yun;Kim, Moo-Key;Ahn, Young-Joon
    • Journal of Applied Biological Chemistry
    • /
    • v.44 no.3
    • /
    • pp.147-153
    • /
    • 2001
  • Methanol extracts from 51 fruits were tested for their fungicidal activities against six phytopathogenic fungi in a greenhouse. The efficacy varied with both the plant pathogen and fruit species used. At 10 and 5 mg/pot, methanol extracts of Poncirus trifoliata peel and seed gave over 80% control values against Pyricularia grisea, and strong fungicidal activities against Rhizoctonia solani were showed from the extracts of Citrus paradisi peel and Punica granatum leaf. In a test with Botrytis cinerea at 5 mg/pot, the extracts of C. sinensis seed and D. kaki leaf produced potent fungicidal activities, and the extracts of C. crenata peel and leaf, Ch. sinensis seed, P. trifoliata peel, and Z. jujuba leaf had strong fungicidal activities. At 5 mg/pot, strong fungicidal activities were produced in the extracts of P. trifoliata peel and seed against Phytophthora infestans and in the extracts of P. ussriensis var. macrostipes fruit and seed, C. crenata peel, C. crenata leaf, C. paradisi peel, P. trifoliata peel, P. granatum peel, and Z. jujuba leaf against Puccinia recondita. In a test with E. graminis, potent activities at 10 mg/pot were produced from the extracts of Ch. sinensis seed, C. sinensis seed, P. trifoliata leaf, P. ussriensis var. macrostipes fruit and seed, and Vitis vinifera seed. In the control effect of seven extracts against B. cinerea strains resistant to carbendazim, procymidone, and diethofencarb, extracts of C. crenata peel and leaf, Ch. sinensis seed, and P. trifoliata peel were highly effective against all strains of B. cinerea. Furthermore, potent fungicidal activities were produced from the extracts of C. sinensis seed and D. kaki leaf against the SSR, SRR, and RRS, and Z. jujuba leaf against the SSR and RRS strains. As a naturally occurring fungicide, these fruit-derived materials could be useful as new fungicidal products against phytopathogenic fungi.

  • PDF

Anti-Inflammatory Effect of Asterias amurensis Fatty Acids through NF-κB and MAPK Pathways against LPS-Stimulated RAW264.7 Cells

  • Monmai, Chaiwat;Go, Seok Hyeon;Shin, Il-sik;You, SangGuan;Kim, Dae-ok;Kang, SeokBeom;Park, Woo Jung
    • Journal of Microbiology and Biotechnology
    • /
    • v.28 no.10
    • /
    • pp.1635-1644
    • /
    • 2018
  • Asterias amurensis (starfish) is a marine organism that is harmful to the fishing industry, but is also a potential source of functional materials. The present study was conducted to analyze the profiles of fatty acids extracted from A. amurensis tissues and their anti-inflammatory effects on RAW264.7 macrophage cells. In different tissues, the component ratios of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids differed; particularly, polyunsaturated fatty acids such as dihomo-gamma-linolenic acid (20:3n-6) and eicosapentaenoic acid (20:5n-3) were considerably different. In lipopolysaccharide-stimulated RAW264.7 cells, fatty acids from A. amurensis skin, gonads, and digestive glands exhibited anti-inflammatory activities by reducing nitric oxide production and inducing nitric oxide synthase gene expression. Asterias amurensis fatty acids effectively suppressed the expression of inflammatory cytokines such as tumor necrosis $factor-{\alpha}$, interleukin-$1{\beta}$, and interleukin-6 in lipopolysaccharide-stimulated cells. Cyclooxygenase-2 and prostaglandin $E_2$, which are critical inflammation biomarkers, were also significantly suppressed. Furthermore, A. amurensis fatty acids reduced the phosphorylation of nuclear $factor-{\kappa}B$ p-65, p38, extracellular signal-related kinase 1/2, and c-Jun N-terminal kinase, indicating that these fatty acids ameliorated inflammation through the nuclear $factor-{\kappa}B$ and mitogen-activated protein kinase pathways. These results provide insight into the anti-inflammatory mechanism of A. amurensis fatty acids on immune cells and suggest that the species is a potential source of anti-inflammatory molecules.

Study on the Attributive Channel Theory of Herbal Medicine by the Pharmacodynamic Research of 1-131 Labelled Hesperetin (Hesperetin의 I-131 표지 및 생체(生體) 분포(分布) 분석(分析)을 통한 귀경(歸經) 연구)

  • Kim, Sung-Kyu;Ham, In-Hye;Bu, Young-Min;Kim, Ho-Cheol;Cho, Jung-Hyuck;Choi, Ho-Young
    • The Korea Journal of Herbology
    • /
    • v.23 no.1
    • /
    • pp.117-125
    • /
    • 2008
  • Objectives : This study was to verify the Attributive Channel theory of herbal medicine. Methods : [$^{131}I$]iodohesperetin was synthesized, separated, and refined from hesperetin, the major component of Citrus species, followed by observing the biodistribution in an organism of C57 BL/6 mice with and without Lewis Lung Carcinoma. Results : Iodohesperetin 27.5 mg was obtained through column cliromatography after a reaction with 50 mg of Hesperitin and 8 mg of Nal. The radiochemical yield of [$^{131}I$]iodohesperetin synthesis was 25 % when checked with Radio TLC chromatography. [131I]iodohesperetin was most largely distributed in the stomach, lung and liver of C57BL/6 mouse. The highest %ID/g in stomach was 40 min, in lung and liver was 20 min after injection. The % ID/g of tumor tissue was comparable with that of blood. Conclusions : The fact that [$^{131}I$]iodocurcumin was most largely distributed in the stomach, lung and liver was related with the Attributive Channel theory. And there was no significant finding related to tumor cells.

  • PDF

Immunomodulatory Activities of Body Wall Fatty Acids Extracted from Halocynthia aurantium on RAW264.7 Cells

  • Monmai, Chaiwat;Jang, A-Yeong;Kim, Ji-Eun;Lee, Sang-Min;You, SangGuan;Kang, SeokBeom;Lee, Tae Ho;Park, Woo Jung
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.12
    • /
    • pp.1927-1936
    • /
    • 2020
  • Tunicates are known to contain biologically active materials and one species in particular, the sea peach (Halocynthia aurantium), has not been thoroughly studied. In this study we aimed to analyze the fatty acids profile of the H. aurantium body wall and its immunomodulatory effects on RAW264.7 macrophage-like cells. The fatty acids were classified into three categories: saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). Omega-3 fatty acid content, including EPA and DHA, was higher than omega-6 fatty acids. H. aurantium body wall fatty acids exhibited enhanced immune response and anti-inflammatory effects on RAW264.7 macrophage-like cells. Under normal conditions, fatty acids significantly increase nitric oxide (NO) and PGE2 production in a dose-dependent manner, thereby improving the immune response. On the other hand, in LPS-treated RAW264.7 cells, fatty acids significantly decreased nitric oxide (NO) and PGE2 production in a dose-dependent manner, thereby enhancing anti-inflammatory effects. Fatty acids transcriptionally control the expression of the immune-associated genes, iNOS, IL-1β, IL-6, COX-2, and TNF-α, via the MAPK and NF-κB signaling cascades in RAW264.7 cells. However, in LPS-stimulated RAW264.7 cells, H. aurantium body wall fatty acids significantly inhibited expression of inflammatory cytokine; similarly, production of COX-2 and PGE2 was inhibited. The results of our present study provide insight into the immune-improving and anti-inflammatory effects of H. aurantium body wall fatty acids on macrophages. In addition, our study demonstrates that H. aurantium body wall is a potential source of immune regulatory components.