• Title/Summary/Keyword: Citron oil : E. coli transformants

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Bioconversion of Citron oil by Co-Culture of E. coli EC3, EC4, and EC6 (E. coli 형질전환주의 공동배양에 의한 유자정유의 생전환)

  • 박연진;장해춘
    • Korean Journal of Human Ecology
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    • v.4 no.1
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    • pp.79-92
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    • 2001
  • E. coli transformants EC3, EC4. and EC6. harboring citron oil degrading pathway genes, were co-cultured in M9 media with citron oil as a sole carbon source at 28$^{\circ}C$. Each co-culture(EC3+EC4, EC3+EC6, EC4+EC6 and EC3+EC4+EC6) showed three to four times higher cell growth than each transformant single culture. Microbial conversion products from the co-cultures were determined by GC-MS. Linalool. 4-terpineol and ${\alpha}$-terpineol were the major common products from co-cultures. Various minor products also were detected and important in flavor characteristics of cultures.

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