• Journal of the Korea Computer Graphics Society
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• v.23 no.4
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• pp.31-40
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• 2017

As 3D cell culture has recently become possible, it has been able to observe a 3D shape of cell and volume. Generally, 3D information of a cell should be observed with a special microscope such as a confocal microscope or an electron microscope. However, a confocal microscope is more expensive than a conventional microscope and takes longer time to capture images. Therefore, there is a need for a method that can reconstruct the 3D shape of cells using a common microscope. In this paper, we propose a method of reconstructing 3D cells using the focus estimator value from multi-focal fluorescence images of cells. Initially, 3D cultured cells are captured with an optical microscope by changing the focus. Then the approximate position of the cells is assigned as ROI (Region Of Interest) using the circular Hough transform in the images. The MSBF (Modified Sliding Band Filter) is applied to the obtained ROI to extract the outlines of the cell clusters, and the focus estimator values are computed based on the extracted outlines. Using the computed focus estimator values and the numerical aperture (NA) of the microscope, we extract the outline of the cell cluster considering the depth and reconstruct the cells into 3D based on the extracted outline. The reconstruction results are examined by comparing with the combined in-focus portions of the cell images.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.12
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• pp.158-164
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• 2015

Lane detection is one of the key parts among autonomous vehicle technologies because lane keeping and path planning are based on lane detection. Camera is used for lane detection but there are severe limitations such as narrow field of view and effect of illumination. On the other hands, Lidar sensor has the merits of having large field of view and being little influenced by illumination because it uses intensity information. Existing researches that use methods such as Hough transform, histogram hardly handle multiple lanes in the co-occuring situation of lanes and road marking. In this paper, we propose a method based on RANSAC and regularization which provides a stable and precise detection result in the co-occuring situation of lanes and road marking in highway scenarios. This is performed by precise lane point extraction using circular model RANSAC and regularization aided least square fitting. Through quantitative evaluation, we verify that the proposed algorithm is capable of multi lane detection with high accuracy in real-time on our own acquired road data.

• International Journal of Contents
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• v.14 no.2
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• pp.24-29
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• 2018

In this paper, we propose an efficient traffic light detection (TLD) method as an advanced driver assistance system (ADAS) for elderly drivers. Since an increase in traffic accidents is associated with the aging population and an increase in elderly drivers causes a serious social problem, the provision of ADAS for older drivers via TLD is becoming a necessary(Ed: verify word choice: necessary?) public service. Therefore, we propose an economical TLD method that can be implemented with a simple black box (built in camera) and a smartphone in the near future. The system utilizes a color pre-processing method to differentiate between the stop and go signals. A mathematical morphology algorithm is used to further enhance the traffic light detection and a circular Hough transform is utilized to detect the traffic light correctly. From the simulation results of the computer vision and image processing based on a proposed algorithm on Matlab, we found that the proposed TLD method can detect the stop and go signals from the traffic lights not only in daytime, but also at night. In the future, it will be possible to reduce the traffic accident rate by recognizing the traffic signal and informing the elderly of how to drive by voice.

• Economic and Environmental Geology
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• v.55 no.5
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• pp.551-561
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• 2022

Geophysical exploration methods are very useful for generating high-resolution images of underground structures, and such methods can be applied to investigation of buried cultural properties and for determining their exact locations. In this study, image feature extraction and image segmentation methods were applied to automatically distinguish the structures of buried relics from the high-resolution ground-penetrating radar (GPR) images obtained at the center of Silla Kingdom, Gyeongju, South Korea. The major purpose for image feature extraction analyses is identifying the circular features from building remains and the linear features from ancient roads and fences. Feature extraction is implemented by applying the Canny edge detection and Hough transform algorithms. We applied the Hough transforms to the edge image resulted from the Canny algorithm in order to determine the locations the target features. However, the Hough transform requires different parameter settings for each survey sector. As for image segmentation, we applied the connected element labeling algorithm and object-based image analysis using Orfeo Toolbox (OTB) in QGIS. The connected components labeled image shows the signals associated with the target buried relics are effectively connected and labeled. However, we often find multiple labels are assigned to a single structure on the given GPR data. Object-based image analysis was conducted by using a Large-Scale Mean-Shift (LSMS) image segmentation. In this analysis, a vector layer containing pixel values for each segmented polygon was estimated first and then used to build a train-validation dataset by assigning the polygons to one class associated with the buried relics and another class for the background field. With the Random Forest Classifier, we find that the polygons on the LSMS image segmentation layer can be successfully classified into the polygons of the buried relics and those of the background. Thus, we propose that these automatic classification methods applied to the GPR images of buried cultural heritage in this study can be useful to obtain consistent analyses results for planning excavation processes.

• Journal of Sensor Science and Technology
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• v.31 no.3
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• pp.175-179
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• 2022

The physical properties of biomaterials are important for their isolation and separation from body fluids. In particular, the precise evaluation of the multi-physical properties of single biomolecules is essential in that the correlation between physical and biological properties of specific biomolecule. However, the majority of scientific equipment, can only determine specific-physical properties of single nanoparticles, making the evaluation of the multi-physical properties difficult. The improvement of analytical techniques for the evaluation of multi-physical properties is therefore required in various research fields. In this study, we developed a motion-tracking algorithm to evaluate the multi-physical properties of single-nanoparticles by analyzing their behavior. We observed the Brownian motion and electric-field-induced drift of fluorescent nanoparticles injected in a microfluidic chip with two electrodes using confocal microscopy. The proposed algorithm is able to determine the size of the nanoparticles by i) removing the background noise from images, ii) tracking the motion of nanoparticles using the circular-Hough transform, iii) extracting the mean squared displacement (MSD) of the tracked nanoparticles, and iv) applying the MSD to the Stokes-Einstein equation. We compared the evaluated size of the nanoparticles with the size measured by SEM. We also determined the zeta-potential and surface-charge density of the nanoparticles using the extracted electrophoretic velocity and the Helmholtz-Smoluchowski equation. The proposed motion-tracking algorithm could be employed in various fields related to biomaterial analysis, such as exosome analysis.