• 한국자기공명학회논문지
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• 제21권2호
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• pp.72-77
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• 2017

${\beta}-catenin$ is a key signaling protein which regulates cell signaling and gene transcription. Abnormal activation of ${\beta}-catenin$ is linked to many cancers, particularly with colorectal cancers. Although many genetic and biological studies on $Wnt/{\beta}-catenin$ have been reported and structures of the complex between ${\beta}-catenin$ and its diverse binding partners have been published, many of them have focused on armadillo repeat domain of ${\beta}-catenin$. Both N- and C-terminal domains have been suggested to regulate interactions of ${\beta}-catenin$ with other molecules, but still little is known about the C-terminal unstructured domain. To investigate the structure of this domain, construct of C-terminus was designed and structural studies were performed using size exclusion chromatography (SEC), circular dichroism (CD), fluorescence and nuclear magnetic resonance (NMR) spectroscopy. We observed that not only the purified full-length construct but the purified C-terminal construct also dimerizes in solution by SEC, suggesting that this domain involves in dimerization of ${\beta}-catenin$. CD and fluorescence data indicate its flexibility and structural formation in the presence of membrane environments.

• 대한조선학회지
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• 제26권2호
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• pp.25-31
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• 1989

정규칙파 중에서의 이차원 주상체의 대진폭 운동이 시간영역 수치계산법에 의해 다루어졌다. 물체표면 경계조건을 각 순간의 실제 물체표면에서 적용하므로 대진폭 운동에서 고려되어야 하는 물체 위치변화 및 형상변화 등에 의한 비선형효과가 고려되고 있으며, 자유표면에서의 비선형효과는 고려되지 않는다. 단순강제동요 및 자유동요문제에 적용된 바 있는 물체 표면 소오스 분포와 자유표면 스펙트럴 표현방법이 입사파의 강제력을 포함하도록 확장 적용되었고 물체의 운동응답은 시간적분법에 의해 계산되었다. 잠수 및 부유주상체의 대진폭 운동이 시간영역에서 직접 시뮬레이션되어 비선형 효과들이 보여지고 있으며, 대진폭 운동시 파강제력에 영향을 미치는 비선형효과가 고찰되어, 물체와 유체입자의 상대운동이 수평 및 수직방향 시간평균력들에 상당한 영향을 미침이 보여지고 있다.

• 대한기계학회논문집B
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• 제28권8호
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• pp.953-960
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• 2004

In this study, the effects of freestream turbulence intensity on laminar-turbulent transition of separated shear layers in the wake of a circular cylinder are investigated using an immersed boundary method and LES. It is shown that the present numerical results without freestream turbulence for Re=3,900 based on bulk mean velocity and the cylinder diameter are in good agreement with other authors' experimental observations and numerical results, verifying our numerical methodology. Then a 'prescribed power spectrum' method is imposed to generate isotropic turbulence at the inlet of the computational domain at each time step. The principal effects of freestream turbulence intensity on flow statistics are investigated for Re=3,900. Statistical study reveals that the Reynolds stresses in the near-wake region gradually increase, and transition occurs further upstream, as the turbulence intensity increases. On the other hand, the bubble size behind the cylinder decreases as the turbulence intensity increases, which indicates that the freestream turbulence helps mean velocity be quickly recovered.

• Fibers and Polymers
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• 제1권1호
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• pp.37-44
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• 2000

A finite element method is employed fer a flow analysis of the melt spinning process of a non-circular fiber, a PET(polyethylene terephthalate) filament. The flow field is divided into two regions of die channel and spin-line. A two dimensional analysis is used for the flow within the die channel and a three dimensional analysis fur the flow along the spin-line. The Newtonian fluid is assumed for the PET melt and material properties are considered to be constant except for the viscosity. Effects of gravitation, air drag force, and surface tension are neglected. Although the spin-line length is 4.5 m only five millimeters from the spinneret are evaluated as the domain of the analysis. Isothermal and non-isothermal cases are studied fer the flow within the die channel. The relationship between the mass flow rate and the pressure gradient is presented for the two cases. Three dimensional flow along the spin-line is obtained by assuming isothermal conditions. It is shown that changes in velocity and cross-sectional shape occur mostly in the region of 1mm from the die exit.

• 한국소음진동공학회논문집
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• 제24권7호
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• pp.569-574
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• 2014

In order to investigate low frequency swishing noise of wind turbines, acoustic source model using a singularity in circular motion is introduced to derive analytic solution of Lowson acoustic analogy in time domain. Results in time and frequency domains computed by the solution show apparent modulation of amplitude and frequency. The solution indicates that time histories of acoustic pressure at receiver points varied significantly according to receiver's directional location, even when the retarded time distributions are similar. However, the corresponding time-averaged spectra of sound pressure at the receiver locations where the retarded time distributions are almost same are not significantly different. It can be inferred from these results that the time-averaged sound pressure spectra which cannot take into account the detailed difference in the time-variation of wind turbine noise may not represent the sound quality of wind turbines due to its swishing. Finally, as an introduction of procedure to quantify low frequency swishing noise level, relative variation of overall sound pressure level is obtained using tonal low frequency noise model.

• 대한기계학회논문집B
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• 제33권2호
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• pp.107-115
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• 2009

A Computational study was carried out in order to investigate the aerodynamic characteristics of circular cylinder moving near the wavy wall at a low Reynolds number of 50. Lattice Boltzmann method was used to simulate the flow field and immersed boundary method was combined to represent the moving cylinder and wavy wall regardless of the constructed grid in the domain. The aerodynamics characteristics of the cylinder moving near the wavy wall were represented by the comparing the lifting coefficients with various altitudes (H/D) and wave length and amplitudes of wavy wall. It indicated that the twice of increasing-decreasing variations of lifting coefficient are obtained while the cylinder moves near the wavy wall. The first variation is obtained where the cylinder locates near the peak of the wavy wall. Another variation occurs when the distance to the wavy wall becomes longer after passing the peak. It was also classified that three different patterns of relation between the lifting and drag coefficient of the cylinder. However, the classification is limited to the case of the same order of altitude, amplitude and wave length of the wavy wall.

• Structural Engineering and Mechanics
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• 제85권2호
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• pp.147-161
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• 2023

Based on the ideas of variational differential quadrature (VDQ) and finite element method (FEM), a numerical approach named as VDQFEM is applied herein to study the large deformations of plate-type structures under static loading with arbitrary shape hole made of functionally graded graphene platelet-reinforced composite (FG-GPLRC) in the context of higher-order shear deformation theory (HSDT). The material properties of composite are approximated based upon the modified Halpin-Tsai model and rule of mixture. Furthermore, various FG distribution patterns are considered along the thickness direction of plate for GPLs. Using novel vector/matrix relations, the governing equations are derived through a variational approach. The matricized formulation can be efficiently employed in the coding process of numerical methods. In VDQFEM, the space domain of structure is first transformed into a number of finite elements. Then, the VDQ discretization technique is implemented within each element. As the last step, the assemblage procedure is performed to derive the set of governing equations which is solved via the pseudo arc-length continuation algorithm. Also, since HSDT is used herein, the mixed formulation approach is proposed to accommodate the continuity of first-order derivatives on the common boundaries of elements. Rectangular and circular plates under various boundary conditions with circular/rectangular/elliptical cutout are selected to generate the numerical results. In the numerical examples, the effects of geometrical properties and reinforcement with GPL on the nonlinear maximum deflection-transverse load amplitude curve are studied.

• Smart Structures and Systems
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• 제19권2호
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• pp.181-194
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• 2017

In order to investigate the interface debonding defects detection mechanism between steel tube and concrete core of concrete-filled steel tubes (CFSTs), multi-physical fields coupling finite element models constituted of a surface mounted Piezoceramic Lead Zirconate Titanate (PZT) actuator, an embedded PZT sensor and a circular cross section of CFST column are established. The stress wave initiation and propagation induced by the PZT actuator under sinusoidal and sweep frequency excitations are simulated with a two dimensional (2D) plain strain analysis and the difference of stress wave fields close to the interface debonding defect and within the cross section of the CFST members without and with debonding defects are compared in time domain. The linearity and stability of the embedded PZT response under sinusoidal signals with different frequencies and amplitudes are validated. The relationship between the amplitudes of stress wave and the measurement distances in a healthy CFST cross section is also studied. Meanwhile, the responses of PZT sensor under both sinusoidal and sweep frequency excitations are compared and the influence of debonding defect depth and length on the output voltage is also illustrated. The results show the output voltage signal amplitude and head wave arriving time are affected significantly by debonding defects. Moreover, the measurement of PZT sensor is sensitive to the initiation of interface debonding defects. Furthermore, wavelet packet analysis on the voltage signal under sweep frequency excitations is carried out and a normalized wavelet packet energy index (NWPEI) is defined to identify the interfacial debonding. The value of NWPEI attenuates with the increase in the dimension of debonding defects. The results help understand the debonding defects detection mechanism for circular CFST members with PZT technique.

• 한국해안해양공학회지
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• 제15권4호
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• pp.232-241
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• 2003

3차원 선형포텐셜 이론아래에서 해저면 바닥에 고정된 N개의 투과성 원기둥과 입사파의 상호작용 문제를 살펴보았다. 유체영역을 때의 외부영역과 N개의 내부영역으로 나누고, 각 유체영역에서의 회절포텐셜을 고유함수전개법에 의해 표현하였다(Williams and Li, 2000). 투과성 구조물은 파력과 처올림 파형을 크게 줄일 수 있다는 사실을 해석결과는 보여주고 있다. 개발된 해석모델을 검증하기 위하여 일렬로 배열한 투과성 원기둥들을 가지고 조파수조(30m $\times$ 7m $\times$ 1.5m)에서 체계적인 모형실험을 수행하였다. 해석결과와 모형실험결과는 정성적으로 잘 일치하고 있음을 확인하였다. 투과성 원기둥을 일렬로 배열하여 만든 방파제는 해수교환뿐 아니라 우수한 소파성능을 가지고 있어 미래의 해수교환방파제로써 무한한 잠재력이 있다고 판단된다.

• BMB Reports
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• 제39권5호
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• pp.530-536
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• 2006

Thermal conformational changes of human serum albumin (HSA) in phosphate buffer, 10 mM at pH = 7 are investigated using differential scanning calorimetric (DSC), circular dichroism (CD) and UV spectroscopic methods. The results indicate that temperature increment from $25^{\circ}C$ to $55^{\circ}C$ induces reversible conformational changes in the structure of HSA. Conformational change of HSA are shown to be a three-step process. Interestingly, melting temperature of the last domain is equal to the maximum value of fever in pathological conditions, i.e. $42^{\circ}C$. These conformational alterations are accompanied by a mild alteration of secondary structures. Study of HSA-SDS (sodium dodecyl sulphate) interaction at $45^{\circ}C$ and $35^{\circ}C$ reveals that SDS affects the HSA structure at least in three steps: the first two steps result in more stabilization and compactness of HSA structure, while the last one induces the unfolding of HSA. Since HSA has a more affinity for SDS at $45^{\circ}C$ compared to $35^{\circ}C$, It is suggested that the net negative charge of HSA is decreased in fever, which results in the decrease of HSA-associated cations and plasma osmolarity, and consequently, heat removal via the increase in urine volume.