• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.1
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• pp.25-35
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• 2010

The biodiversity of eukaryotic plankton has commonly been used to evaluate the status of aquatic ecosystems. Therefore, an accurate and rapid method for species identification is needed to reveal the biodiversity of environmental water samples. To date, molecular methods have provided a great deal of information that has enabled identification of the hidden biodiversity in environmental samples. In this study, we utilized environmental polymerase chain reaction (PCR) and constructed the 18S nuclear ribosomal RNA clone library from environmental water samples in order to develop more efficient methods for species identification. For the molecular analysis, water samples were collected from the Seonakdong River (Gimhae Bridge) and the coast of Namhae，(Namhaedo). Colony PCR and restriction fragment length polymorphism of PCR (PCR-RFLP) were then adopted to isolate unique clones from the 18S rDNA clone library. Restriction fragment length polymorphism pattern analysis of the Gimhae Bridge sample revealed 44 unique clones from a total of 60 randomly selected clones, while analysis of the Namhae sample revealed 27 unique clones from 150 clones selected at random. A BLAST search and subsequent phylogenetic analysis conducted using the sequences of these clones revealed hidden biodiversity containing a wide range of taxonomic groups (Heterokontophyta (7), Ciliophora (23), Dinophyta (1), Chytridiomycota (1), Rotifera (1) and Arthropoda (11) in the Gimhae Bridge samples Ciliophora (4), Dinophyta (3), Cryptophyta (1), Arthropoda (19) in the Namhae samples). Therefore, the molecular monitoring method developed here can provide additional information regarding the biodiversity and community structure of eukaryotic plankton in environmental samples and helps construct a useful database of biodiversity for aquatic ecosystems.

• Animal Systematics, Evolution and Diversity
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• v.21 no.1
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• pp.21-30
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• 2005

The morphogenesis of the marine ciliate, Pelagostrobilidium simile Song and Bradbury, 1998, was investigated using pyridine silver carbonate impregnation. The morphogenesis of P. simile is of hypoapokinetal mode. The oral primordium of P. simile commences slightly below the external membranelles (EM) with the proliferation of an anarchic field. Somatic ciliature in proter and opisthe of P. simile are derived from the old structure with the proliferation of the basal bodies during the dividing process. Parental oral apparatus of P. simile is inherited by the proter, and no reorganization of oral apparatus was observed in the parental oral infraciliature.

• Journal of Species Research
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• v.11 no.4
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• pp.335-342
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• 2022

Through the 'Discovery of Korean Indigenous Species' project hosted by the National Institute of Biological Resources (NIBR), new or unrecorded species of various protozoans inhabiting the Korean Peninsula have been discovered. Samples were collected from marine, freshwater, and soil habitats in South Korea and all species were identified and reviewed by experts working on the respective taxonomic group. This study includes 17 unrecorded Korean protozoan species discovered through this project, which belong to four phyla: Amoebozoa Lühe, 1913, Cercozoa Cavalier-Smith, 1998, Euglenozoa Cavalier-Smith, 1981, and Ciliophora Doflein, 1901. Among them, three families (Rhogostomidae Dumack et al., 2017, Parauronematidae Small and Lynn, 1985, and Cyclidiidae Ehrenberg, 1838) and three genera (Rhogostoma Belar, 1921, Parauronema Thompson, 1967, and Cyclidium Müller, 1773) were reported for the first time in Korea. Unlike the previous paper of 2017, this study provides a comprehensive taxonomic account of each species (e.g., species name, collection site, synonyms, specimen vouchers, diagnoses, and figures). Additionally, all the species were assigned Korean names.

• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.189-196
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• 2015

We verified three ciliates - Dysteria brasiliensis Faria et al., 1922, Dysteria lanceolata ${Clapar\grave{e}ede}$ and Lachmann, 1859, and Dysteria ovalis (Gourret and Roeser, 1886) Kahl, 1931 - as previously unrecorded species in Korea. These three ciliates were collected from Incheon Harbor and Geojin Harbor in Korea. We describe the three species based on live observations, protargolimpregnated specimens, and silver nitrate-impregnated specimens.

• Journal of Species Research
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• v.5 no.3
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• pp.514-516
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• 2016

Three marine and one brackish-water ciliates (Dysteria crassipes $Clapar\grave{e}de$ and Lachmann, 1859; Gruberia calkinsi $Beltr\acute{a}n$, 1933; Pleuronema salmastra Dragesco, 1986; Zosterodasys agamaliev Deroux, 1978) were isolated from Incheon in Korea and their morphologies were investigated using live observation and protargol impregnation.

• Journal of Species Research
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• v.5 no.3
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• pp.343-347
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• 2016

Two pleurostomatid ciliates, Amphileptus litonotiformis Song, 1991 and Kentrophyllum setigerum (Quennerstedt, 1867), were collected from the coastal waters of Korea. Their morphologies are described based on live observation and protargol impregnation, and their diagnoses and morphometrics are provided. Additionally, sequence information of small subunit ribosomal RNA obtained from two species were reported.

• Journal of Species Research
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• v.5 no.3
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• pp.463-467
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• 2016

We collected two unrecorded Colpoda species - C. cucullus $M\ddot{u}ller$, 1773 and C. inflata (Stokes, 1885) Kahl, 1931 from Jeju-do Province (Sarabong Park, $33^{\circ}31^{\prime}N$ $126^{\circ}32^{\prime}E$) in November, 2014. We described these species based on observations of live and impregnated (protargol and silver nitrate impregnation) specimens.

• Journal of Species Research
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• v.5 no.3
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• pp.468-476
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• 2016

We firstly report three stichotrichs, Metaurostylopsis cheni Chen, Huang & Song, 2010, Gonostomum strenuum (Engelmann, 1862) Sterki, 1878, and Lamtostyla granulifera Foissner, 1997, collected in South Korea. We investigated these ciliates based on observations of live and protargol-impregnated specimens.

• Journal of Species Research
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• v.6 no.spc
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• pp.15-25
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• 2017

Twelve ciliates were collected from freshwater, marine, and brackish water habitats in Korea and taxonomic descriptions are provided based on observations of living cells and protargol-impregnated specimens. These ciliates, recorded for the first time in Korea, were classified into 12 genera, 11 families, 10 orders, and five classes. We provide brief descriptions and remarks, including microphotographs, for the 12 ciliates: Allotricha mollis, Amphileptus eigneri, Didinium gargantua, Holophrya teres, Lacrymaria marina, Novistrombidium apsheronicum, Pelagostrobilidium conicum, Pseudochilodonopsis fluviatilis, Pseudourostyla subtropica, Strombidium conicum, Urocentrum turbo, and Uronema marinum.

• Journal of Species Research
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• v.6 no.spc
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• pp.110-113
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• 2017

We collected three soil ciliates, Colpoda maupasi Enriquez, 1908, Tillina minima Alekperov, 1985, and Gonostomum singhii Kamra et al., 2008, in Korea. The two genera, Colpoda and Tillina, belong to the class Colpodea, and the other, Gonostomum belongs to the class Spirotrichea. We describe these species based on observations of live and protargol-impregnated specimens.