• Title/Summary/Keyword: Chysomelidae

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Immature Stages of Korean Thiaspida Weise (Coleoptera: Chrysomelidae) (한국산 큰남생이잎벌레속(Thlaspida)의 미성숙 단계에 관한 연구(딱정벌레목: 잎벌레과))

  • 이종은;박중직
    • The Korean Journal of Zoology
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    • v.39 no.4
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    • pp.387-392
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    • 1996
  • All immature stages, eggs, larvae, and pupae of Korean Thiaspida, T. cribrosa and T. lewisii, are described and Illustrated for the first time. Two species belonging to genus Thiaspida In the subfamily Cassidlnae; T. cribrosa and T. lewisii.Observatiors on bionomics of immature stages and on their taxonomic charaders are also given.

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Geographic Genetic Contour of A Leaf Beetle, Chrysolina aurichalcea (Coleoptera: Chysomelidae), on the Basis of Mitochondrial COI Gene and Nuclear ITS2 Sequences

  • Park, Joong-Won;Park, Sun-Young;Wang, Ah-Rha;Kim, Min-Jee;Park, Hae-Chul;Kim, Ik-Soo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.23 no.1
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    • pp.155-166
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    • 2011
  • The leaf beetle, $Chrysolina$ $aurichalcea$ (Coleoptera: Chysomelidae), is a pest damaging plants of Compositae. In order to understand the genetic diversity and geographic variation we sequenced a portion of mitochondrial COI gene (658 bp) and complete nuclear internal transcribed spacer 2 (ITS2) of the species collected from seven Korean localities. A total of 17 haplotypes (CACOI01~CACOI17), with the maximum sequence divergence of 3.04% (20 bp) were obtained from COI gene sequence, whereas 16 sequence types (ITS2CA01~ITS2CA16), with the maximum sequence divergence of 2.013% (9 bp) were obtained from ITS2, indicating substantially larger sequence divergence in COI gene sequence. Phylogenetically, the COI gene provided two haplotype groups with a high nodal support (${\geq}87%$), whereas ITS2 provided only one sequence type group with a high nodal support (${\geq}92%$). The result of COI gene sequence may suggest the presence of historical biogeographic barriers that bolstered genetic subdivision in the species. Different grouping pattern between COI gene and ITS2 sequences were interpreted in terms of recent dispersal, reflected in the ITS2 sequence. Finding of unique haplotypes and sequence types only from Beakryeng-Islet population was interpreted as an intact remnant of ancient polymorphism. As more samples are analyzed using further hyper-variable marker, further fruitful inference on the geographic contour of the species might be available.