• Title/Summary/Keyword: Chungnam Library

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Cloning and Expression of Pseudomonas cepacia catB Gene in Pseudomonas putida

  • Song, Seung-Yeon;Jung, Young-Hee;Lee, Myeong-Sok;Lee, Ki-Sung;Kim, Young-Soo;Kim, Chi-Kyung;Choi, Sang-Ho;Min, Kyung-Hee
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.334-340
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    • 1996
  • The enzyme, cis,cis-muconate lactonizing enzyme has been proposed to play a key role in the $\beta$-ketoadipate pathway of benzoate degradation. A 3.2-kb EcoRI fragment termed as pRSU2, isolated from a Pseudomonas cepacia genomic library was able to complement the catB defective mutant. Several relevant restriction enzyme sites were determined within the cloned fragment. In Pseudomonas putida SUC2 carrying pRSU2, the enzyme activity was relatively higher than those of the induced or partially induced state of wild type P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida. One possible interpretation of these results is that the catB promoter in P. cepacia is recognized within P. putida, resulting in the almost same expression level.

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Disambiguation of Korean Names in References

  • Kim, Sungwon
    • Journal of Information Science Theory and Practice
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    • v.6 no.2
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    • pp.62-70
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    • 2018
  • One of the characteristics of academic writing is the inclusion of citations and references. As the development of reference styles used for international scholarly communication has mostly been led by Western academic societies, the reference styles developed in Western nations do not reflect the characteristics of Korean names. As a result, it is hard to distinguish Korean authors through citations based on Western reference styles, which in turn decreases the retrieval efficiency of relevant authors and ultimately the efficiency of scholarly communication. This paper intends to analyze author name disambiguation of Korean authors indicated according to Western reference styles. It aims to suggest the necessity for enhancing name disambiguation of Korean authors and revision of reference styles. Its ultimate goal is to increase the efficiency of scholarly communication through the improvement of name disambiguation of Korean authors. For this purpose, this study collected and analyzed name data of Korean researchers and compared name disambiguation of authors by reference style. Based on research results, this study confirmed a necessity for revising reference styles to improve name disambiguation of authors and suggested a necessity for research into the improvement of plans for revision.

Radiometric Calibration of FTIR Spectrometer For Passive Remote Sensing Application (수동형 원격탐지 FTIR 분광계의 Radiometric Calibration)

  • Kim, Dae-Sung;Park, Do-Hyun;Choi, Seung-Ki;Ra, Sung-Woong
    • Korean Journal of Optics and Photonics
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    • v.17 no.5
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    • pp.391-395
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    • 2006
  • In this paper, radiometric calibration of a FTIR spectrometer for passive remote sensing application was introduced and verified. Radiometric calibration is a significant signal processing procedure to retrieve the object radiance from the measured spectrum. The object radiance is measured and registered distorted by the detector's responsivity dependent on wavelength and instrument self-emission. Radiance of two temperature points, hot temperature and cold temperature, from a well-controlled blackbody was measured and used to obtain the scale factor and offset factor which are required for radiometric calibration. For gas phase C2H5OH. radiometric calibration was done and verified through comparison of its emission line width and intensity with the standard spectrum.

Intake Status and Perception of Energy Drinks according to the Gender and Obesity of Some University Students in Chungnam Province (충남지역 일부 대학생의 성별과 비만도에 따른 에너지음료 섭취실태 및 인식)

  • Ko, Kab-Keum;Kim, Myung-Hee;Lee, Je-Hyuk
    • The Korean Journal of Food And Nutrition
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    • v.32 no.3
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    • pp.167-178
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    • 2019
  • The purpose of this study was to investigate the intake status of energy drinks, knowledge of caffeine and the perception on intake of energy drinks by university students. We surveyed 351 university students from October 25, 2017 to January 25, 2018. The subjects (70.9%) consumed energy drinks more than once. Male students consumed energy drinks more frequently than the female ones. A huge portion (68.3%) of the subjects consumed energy drinks to recover from fatigue and reduce sleepiness. Moreover, they consumed most of the energy drinks at home and the university library. The main anticipated efficacy of energy drinks was the relieving drowsiness. Respondents (86.3%) perceived that energy drinks could be hazardous to the health of human beings. The main risks of consuming energy drinks were sleep disorders, addiction problems and heart beating. The main recognized effects of energy drinks were relieved drowsiness and decreased fatigue. The major side effects were heart beating, nausea, vomiting, heartburn and sleeping disorders. This study suggests that nutritional education is needed to confirm the recommendation of daily intake for caffeine and to raise awareness about the side effects of energy drinks.

Data Scholarship: Data Journals and Data Repositories (데이터 스칼라십: 데이터 저널과 데이터 리포지토리를 중심으로)

  • Hyoungjoo Park
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.1
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    • pp.443-451
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    • 2024
  • The purpose of this study is to comprehend the knowledge structure of data scholarship within data journals and repositories. The study explored various aspects, including types of peer review, co-occurrence analysis through author keywords, and network analysis via article titles. The majority of data repositories in the DCI are maintained by countries in North America and the European Union. In Korea, data repositories are predominantly managed by research institutions. This study contributes to enhancing our understanding of the practices in data scholarship.

Preparation of Conjugated Linolenic Acid from Urea Fractionated Perilla Seed Oil Hydrolysate (우레아 분별된 들기름 가수 분해물을 이용한 Conjugated Linolenic Acid(CLnA)의 합성)

  • Lee, Kyung-Su;Shin, Jung-Ah;Lee, Ki-Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.12
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    • pp.1734-1742
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    • 2011
  • Conjugated linolenic acid (CLnA) and conjugated linoleic acid (CLA) are positional geometric isomers with three and two double bonds, respectively. In this study, perilla seed oil containing 60% ${\alpha}$-linolenic acid (C18:3) and 30% linoleic acid (C18:2) was used as a reaction substrate. After the perilla seed oil was hydrolyzed, conjugated fatty acids were synthesized using different reaction parameters, such as reaction time and concentration of sodium hydroxide. As a result, CLnA, CLA, and other newly synthesized conjugated isomers were present at levels of 14.5%, 14%, and 42.2%, respectively, when the reaction was performed with 20% NaOH, at $180^{\circ}C$, and for 1 hr. The results of GC-MS and fourier transform infrared spectroscopy (FT-IR) showed that CLnA isomer of cis-9, trans-11, and trans-13 octadecatrienoate, CLA isomer of cis-9, trans-11, and trans-10, cis-12 octadecadienoate, and other conjugated isomers were produced. Using urea, ${\alpha}$-linolenic acid could be concentrated from perilla seed oil hydrolysate. After concentration by urea, the concentration of ${\alpha}$-linolenic acid reached about 70%. After alkaline-isomerization was performed on the urea fraction containing 70% ${\alpha}$-linolenic acid, the content of CLnA increased up to 16.6%.

Analysis of Research Trends in Cooperation between Industry and Specialized High School (특성화 고등학교 산학협동 관련 연구 동향 분석)

  • Kim, Young-Min;Kim, Hyun-Jeong;Lee, Chang-Hoon;Kim, Ki-Soo
    • 대한공업교육학회지
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    • v.36 no.1
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    • pp.95-114
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    • 2011
  • The purpose of this study was to review and synthesize the papers in cooperation between industries and specialized high schools. We also try to provide explicit directions and suggestions for future researches. A total of 196 studies, including master's and doctoral theses up to 2010, were collected through RISS(Research Information Sharing Service), KISS(Koreanstudies Information Service System), and DBPIA(special database for domestic research information). The results were as follows. The number of papers has been increasing steadily. In the area of research, the field research was the most popular area. The most common type of the research was survey research and library research was followed. Similarly, questionnaire was the most popular method. Technical high schools were the most popular object of studies in specialized high schools. GyeongGi province was the most popular region of studies and Seoul was followed. The most common type of cooperation between industries and specialized high schools were actual training. The students in specialized high school was the most popular object of surveys, and teachers and industry people were followed.

Korea Brassica Genome Project: Current Status and Prospective (배추 유전체열구의 현황과 전망)

  • Choi, Su-Ryun;Park, Jee-Yong;Park, Beom-Seok;Kim, Ho-Il;Lim, Yong-Pyo
    • Journal of Plant Biotechnology
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    • v.33 no.3
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    • pp.153-160
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    • 2006
  • Brassica rape is an important species used as a vegetable, oil, and fodder worldwide. It is related phylogenically to Arabidopsis thaliana, which has already been fully sequenced as a model plant. The 'Multinational Brassica Genome Project (MBGP)'was launched by the international Brassica community with the aim of sequencing the whole genome of B. rapa in 2003 on account of its value and the fact that it has the smallest genome among the diploid Brassica. The genome study was carried out not only to know the structure of genome but also to understand the function and the evolution of the genes comprehensively. There are two mapping populations, over 1,000 molecular markers and a genetic map, 2 BAC libraries, physical map, a 22 cDHA libraries as suitable genomic materials for examining the genome of B. rapa ssp. pekinensis Chinese cabbage. As the first step for whole genome analysis, 220,000 BAC-end sequences of the KBrH and KBrB BAC library are achieved by cooperation of six countries. The results of BAC-end sequence analysis will provide a clue in understanding the structure of the genome of Brassica rapa by analyzing the gene sequence, annotation and abundant repetitive DHA. The second stage involves sequencing of the genetically mapped seed BACs and identifying the overlapping BACs for complete genome sequencing. Currently, the second stage is comprises of process genetic anchoring using communal populations and maps to identify more than 1,000 seed BACs based on a BAC-to-BAC strategy. For the initial sequencing, 629 seed BACs corresponding to the minimum tiling path onto Arabidopsis genome were selected and fully sequenced. These BACs are now anchoring to the genetic map using the development of SSR markers. This information will be useful for identifying near BAC clones with the seed BAC on a genome map. From the BAC sequences, it is revealed that the Brassica rapa genome has extensive triplication of the DNA segment coupled with variable gene losses and rearrangements within the segments. This article introduces the current status and prospective of Korea Brassica Genome Project and the bioinformatics tools possessed in each national team. In the near future, data of the genome will contribute to improving Brassicas for their economic use as well as in understanding the evolutional process.

Molecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress

  • Kim, Yun-Hee;Yang, Kyoung-Sil;Kim, Cha-Young;Ryu, Sun-Hwa;Song, Wan-Keun;Kwon, Suk-Yoon;Lee, Haeng-Soon;Bang, Jae-Wook;Kwak, Sang-Soo
    • BMB Reports
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    • v.41 no.3
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    • pp.259-265
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    • 2008
  • Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of the pI values of mature proteins. Fluorescence microscope analysis indicated that three PODs are secreted into the extracellular space. RT-PCR analysis revealed that POD genes have diverse expression patterns in a variety of plant tissues. Swpb4 was abundantly expressed in stem tissues, whereas the expression levels of swpb5 and swpa7 transcripts were high in fibrous and thick pigmented roots. Swpb4 and swpa7 showed abundant expression levels in suspension cultured cells. Three POD genes responded differently in the leaf and fibrous roots in response to a variety of stresses including dehydration, temperature stress, stress-associated chemicals, and pathogenic bacteria.

Analysis of Volatile Components of the Musk of Ondatra zibethicus by Gas Chromatography-Mass Spectrometry (가스크로마토그래피-질량분석기를 이용한 서사향의 향기성분 분석)

  • Jin, Yong-Xie;Choi, Se-Jin;Jung, Eui-Jin;Kim, Sun-Ju;Jeon, Young-Eun;Kang, Il-Jun;Chung, Cha-Kwon;Lim, Soon-Sung
    • Korean Journal of Pharmacognosy
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    • v.40 no.4
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    • pp.303-308
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    • 2009
  • Essential oils of the musk from wild muskrat (Ondatra zibethicus) were analyzed by gas chromatography/mass spectrometry. Fifty-six chemical components were separated, and 14 volatile compounds (78% of composition of total essential oil) of macrocyclic musk, mainly cyclopentadecanone, 12-hydroxy-14-methyl-oxa-cyclotetradec-6-en-2-one, cycloheptadecanone and 9-cycloheptadecen-1-one, were identified by the comparison of Wiley/NBS Library from the results of GC-MS. However, l-muscone, a compound reported by earlier workers in the musk from muskrat, was not detected using the spike test in this study. Interestingly, cyclohexadecanone, which has the same molecular weight (Mw 238) as l-muscone, was identified. In addition, it is considered that because of possessing much higher amounts of macrocyclic components compared to the original musk, muskrat has higher economic values in perfume and pharmaceutical industries.