• Title/Summary/Keyword: Chu-Na

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Genotypic Diversity of the Complete Open-Reading Frame 7 Sequences of Porcine Reproductive and Respiratory Syndrome Viruses in Korea and Coexistence of Two Genotypes

  • Chu, Jia-Qi;Kim, Myung-Cheol;Park, Chang-Sik;You, Myung-Jo;Jun, Moo-Hyung
    • Journal of Veterinary Clinics
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    • v.25 no.3
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    • pp.139-145
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    • 2008
  • To investigate the genotypic diversity of the porcine reproductive and respiratory syndrome viruses (PRRSV) in Korea, we examined 92 clinical samples from three provinces by RT-PCR and a nested PCR, and the complete open-reading frame 7 (ORF 7) sequences of 15 samples selected from 72 PCR-positive specimens were analyzed. When we compared nucleotide (amino acid) sequences of 80 isolates from Korea and overseas countries, the sequences of 7 samples belonged to North American (NA)-genotype, and those of 8 samples, to European (EU)-genotype. The nucleotide (amino acid) identities between two genotypes were 63.7% (59.8%) to 65.1% (63.1%). When compared with NA prototype VR-2332, the 7 strains of NA-genotype shared 89.8% (93.6%) to 91.2% (96.0%) identity of nucleotide (amino acid) sequence. The 8 strains of EU-type shared 93.6% (92.3%) to 94.3% (93.8%) identity of nucleotide (amino acid) sequence as compared to EU prototype Lelystad. In phylogenetic tree analysis by neighbor-joining method, all of the 8 EU-type strains were clustered into group 4 distinct from ED-prototype Lelystad (group 1). In NA-genotype, 24 domestic isolates reported previously and the 7 strains of NA-type determined in this study were clustered into group 1, while US prototype VR 2332 was classified into different group (group 2). These results suggest that emergence of EU-genotype and the dual-infection of NA- and EU-genotypes may be prevalent in the pig farms in Korea. The high degree of genetic diversity of field PRRSVs should be taken into consideration for control and preventive measures.

Investigation of Affect on Composting process and plant growth of Salt concetration in food waste (음식물쓰레기 염분(NaCl)농도가 퇴비화 및 식물성장에 미치는 영향)

  • Phae, Chae-Gun;Chu, Yo-Sub;Park, Jeong-Soo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.4
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    • pp.103-111
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    • 2002
  • The purpose of this study is to investigate influence of NaCl precisely in recycling process of food waste and in places where food waste compost is used, because NaCl with food waste has caused concerns of adverse effects in the process of food waste recycling and in plates where final products is used. The influence of NaCl in composting was measured through temperature, concentration of carbon dioxide ($CO_2$) and pH. NaCl didn't cause bad effects until 1%(wet base) of NaCl concentration for composting, showed adverse effects gradually at 2% and 3% and affected composting deeply at 6%. The influence of NaCl in composting was insignificant until 3% of NaCl concentration but decomposing efficiency of organic compounds was interfered with more than 3%. NaCl concentration has to be maintained less than 1%(wet base) to minimize adverse effects of NaCl in composting however NaCl concentration with food waste used in real composting facilities is so lower than 1 %(wet base) that the bad influence in food waste composting is slight. Compost(final product) was collected from real food waste recycling facilities in A city, S city, K-Gu and S-Gu and controlled NaCl concentration artificially such as 0.5%, 0.8%, 1.2%, 1.6%, 2.0%, 2.5% and 3.0% to investigate influence of NaCl on growth of plants. In results of plant test and pot experiment, according to quality of compost sampled, the influence of NaCl is insufficient until 3% and bad effects appeared clearly more than 3%. The bad effects showed less than 3% because compost sampled was not stabilized fully.

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Hydrogen Supply to PEMFC for Unmanned Aero Vehicles Using Hydrolysis Reaction of NaBH4 (NaBH4 가수분해 반응에 의한 무인항공기용 PEMFC 수소공급)

  • Jung, Hyeon-Seong;Jo, Byung-Joo;Lee, Jung-Hoon;Lee, Han-Jong;Na, Il-Chai;Chu, Cheun-Ho;Park, Kwon-Pil
    • Korean Chemical Engineering Research
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    • v.54 no.1
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    • pp.11-15
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    • 2016
  • Proton Exchange Membrane Fuel Cells (PEMFC) instead of batteries is appropriate for long time flight of unmanned aero vehicles (UAV). In this work, $NaBH_4$ hydrolysis system supplying hydrogen to PEMFC was studied. In order to decrease weight of $NaBH_4$ hydrolysis system, enhancement of hydrogen yield, recovery of condensing water and maintenance of stable hydrogen yield were studied. The hydrogen yield of 3.4% was increased by controlling of hydrogen pressure in hydrolysis reactor. Condensing water formed during air cooling of hydrogen was recovered into storage tank of $NaBH_4$ solution. In this process the condensing water dissolved $NaBH_4$ powder and then addition of $NaBH_4$ solution decreased system weight of 14%. $NaBH_4$ hydrolysis system was stably operated with hydrogen yield of 96% by 2.0g Co-P-B catalyst for 10 hours at 2.0L/min hydrogen evolution rate.

Studies on the Yeasts for the Brewing of Soy sauce (Part 6) Nutritional requirements of Saccharomyces rouxii $T_9$ (2) -Influence of Addition and Osmotic Characteristics of Sugars- (간장발효에 관여하는 효모에 관한연구(제6보) Saccharomyces rouxii $T_9$의 영양요구성(2) -당류첨가의 영향과 내당성에 대하여-)

  • Lee, Taik-Soo;Lee, Suk-Kun;Chu, Young-Ha
    • Applied Biological Chemistry
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    • v.14 no.2
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    • pp.117-120
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    • 1971
  • The effect and osmotic characteristics of sugars for the growth of Saccharomyces rouxii $T_9$ were examined and their results obtained were as follows. (1) The best effective sugars for the growth of Saccharomyces rouxii $T_9$ were inulin, fructose and maltose. (2) Glucose, saccharose, galactose and arabinose were more effective on the media containing 20 percent of NaCl than containing none of NaCl, while fructose, inulin, xylose, sorbitol, raffinose, mannitol, mannose, trehalose and rhamnose were more effective on the media containing none of NaCl. (3) Maltose and lactose were excellent for the growth of Saccharomyces rouxii $T_9$ as addition of 5 to 10 percent on the media containing NaCl and more good in case of 15 percent or more addition on the media containing none of NaCl. (4) Saccharomyces rouxii $T_9$ was generally grown well on the media at the range of containing 15 to 30 percent of sugar concentration. (5) Saccharomyces rouxii $T_9$ was grown up to 80 percent concentration of glucose and 90 of saccharose and maltose.

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Effect of Gamma Irradiation on Botrytis cinerea Causing Gray Mold and Cut Chrysanthemum Flowers

  • Chu, Eun-Hee;Shin, Eun-Jung;Park, Hae-Jun;Jeong, Rae-Dong
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.193-200
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    • 2015
  • Gray mold caused by Botrytis cinerea is one of the most important postharvest fungal pathogens of cut flowers. Here, gamma irradiation, an alternative for phytosanitary purposes, and sodium dichloroisocyanurate (NaDCC) were used to control B. cinerea in a cut chrysanthemum (Chrysanthemum morifolium Ramat.) cultivar, 'Baekma', one of the cultivars susceptible to B. cinerea. Spore germination and mycelium growth of B. cinerea were inhibited by gamma irradiation in an inversely dose-dependent manner. A dose of 4 kGy completely inhibited the mycelium growth of B. cinerea. A significant change in flower quality (physical properties) on chrysanthemum was shown from gamma irradiation at over 0.2 kGy (p<0.05). Therefore, in this study, the integration of gamma ray (below 0.2 kGy) and NaDCC, an eco-friendly form of chlorine, was investigated to control the disease with low dose of gamma irradiation dose. Interestingly, the gamma irradiated flowers showed more disease severity than the non-irradiated flowers. The combined treatment of gamma irradiation and NaDCC does not affect the severity of the fungal disease, whereas only 70 ppm of NaDCC treatment showed a significantly reduced severity. These results suggest that only chlorination treatment can be applied to control B. cinerea in cut chrysanthemum flowers.

Fabrication of Mg(OH)2 from Ferro-Nickel Slag (페로니켈 슬래그를 이용(利用)한 수산화(水酸化)마그네슘 제조방법(製造方法))

  • Park, Soo Hyun;Chu, Yong Sik;Song, Hun;Lee, Jong Kyu;Seo, Sung Kwan
    • Resources Recycling
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    • v.22 no.1
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    • pp.42-47
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    • 2013
  • Ferro-Nickel slag is a byproduct of Ferro-Nickel manufacturing process. Ferro-Nickel slag mostly discarded or used as aggregates despite having useful ingredients such as magnesium oxide and silicon oxide. This study tried to extract process for Mg ion using $H_2SO_4$ solution. And remove impurities and get high purity $Mg(OH)_2$ using NaOH. Mg ion was extracted with the Fe ion and other Ferro-Nickel slag composition by $H_2SO_4$ solution. It is important to control the pH because remove impurities and obtain high-purity $Mg(OH)_2$. The impurities were removed by precipitation of the hydroxides. After this process, we added NaOH and high-purity $Mg(OH)_2$ was obtained.

The Effect of Paljeong-San Pharmacopuncture Treatment on Glycerol-Induced Acute Renal Failure in Rats (팔정산(八正散) 약침(藥鍼)이 글리세롤로 유발된 흰쥐의 급성 신부전에 미치는 영향)

  • Lee, So-Young;Kim, Min-Ho;Yun, Yeo-Choong;Cho, Su-In;Lim, Se-Hyun
    • Journal of Haehwa Medicine
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    • v.21 no.1
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    • pp.163-174
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    • 2012
  • Objective: The primary objective this study was to evaluate the effect of Paljeong-san (PJS) pharmacopuncture treatment on against the glycerol-induced acute renal failure in rats. Methods: Glycerol injection decreased GFR (glomerular filtration rate) and increased urine volume, serum creatinine, BUN level, urine albumine secretion and fractional excretion of Na+, K+. PJS was selected in the basis of invigorating kidney which can eliminate pathogens. Rats were treated with PJS pharmacopuncture on Shin-shu (BL23) and Chon-chu (ST25) point for 3 days, followed by 50% concentration of glycerol injection ($5m{\ell}/kg$ body weight). Results: After the 3 days treatment period, Paljeong-san (PJS) pharmacopuncture treatment improved renal function. In addition, Glycerol injection increased lipid peroxidation, and decreased Na-K-ATPase in renal cortex and which were prevented by PJS treatment. Conclusion: This study suggests that Paljeong-san (PJS) pharmacopuncture treatment show favorable effect on glycerol-induced acute renal failure in rats.

Studies on the Photo-electrochemical Properties of Ti$O_{2-x}$ Thin Films (Ti$O_{2-x}$ 박막의 광-전기화학적 성질에 관한 연구)

  • Q Won Choi;Chu Hyun Choe;Ki Hyung Chjo;Yong Kook Choi
    • Journal of the Korean Chemical Society
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    • v.29 no.6
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    • pp.582-591
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    • 1985
  • The thin films of Ti$O_{2-x}$, were prepared by vapor oxidation and Ti$O_2$ single crystal was reduced by heating in argon atmosphere. The photo-electrochemical properties of these samples were studied. When the photocurrent was scanned in 1M NaOH electrolyte solution, several peaks were observed in the vicinity of 320nm in the UV-region and in the vicinity of 520nm, 620nm, and 740nm in the visible-region. Contrary to the previous suppositions, those peaks were produced by the second-order lines from the grating monochromator.

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Experimental Study on Thermochemical Water Splitting Hydrogen Production Using $MnO_2/Mn_2O_3$/NaOH System Added with $ZrO_2$ ($ZrO_2$ 첨가 $MnO_2/Mn_2O_3$/NaOH 계를 이용한 열화학적 물분해 수소제조 실험 연구)

  • Cha, Kwang-Seo;Ryu, Jae-Chun;Lee, Dong-Hee;Kim, Young-Ho;Park, Chu-Sik;Kim, Jong-Won
    • Transactions of the Korean hydrogen and new energy society
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    • v.17 no.4
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    • pp.353-361
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    • 2006
  • As one of the thermochemical water splitting hydrogen production cycles, which could be operated at the lower temperature below 1200 K, we investigated the feasibility of the cyclic operation of Ispra Mark 2 cycle with the addition of $ZrO_2$. The cycle is theoretically composed of three reaction steps; (1) 1st step($2MnO_2{\rightarrow}Mn_2O_3+0.5O_2$), (2) 2nd step($Mn_2O_3+4NaOH{\rightarrow}2Na_2O{\cdot}MnO_2+H_2+H_2O$) and (3) 3rd step($2Na_2O{\cdot}MnO_2H_2O{\rightarrow}4NaOH+2MnO_2$). From the TPR tests, the temperature ranges for $O_2$ production in 1st step and $H_2$ production in 2nd step were $550{\sim}750^{\circ}C$ and $650{\sim}750^{\circ}C$, respectively. In $MnO_2/Mn_2O_3/NaOH$ system, the formation of molten products due to the reaction between manganese oxides and NaOH were greatly decreased with the addition of $ZrO_2$. In addition, the results of a cyclic test were discussed with the viewpoint of $H_2$ production amounts and the feasibility of the process improvement.

The Effect of the Polygonum tinctoria Niram on Atopic Dermatitis in Dinitrochlorobenzene-Induced BALB/c Mice

  • Chu, Han-Na;Kim, Jeong-Sang
    • Applied Microscopy
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    • v.44 no.2
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    • pp.53-60
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    • 2014
  • In the present study, we investigated the effects of Polygonum tinctoria Niram (PTN) on atopic dermatitis (AD) in BALB/c mice induced by 2,4-dinitrochlorobenzene (DNCB). They were divided into four groups; Control, DNCB, DNCB+1%PTN (1% PTN extract) and DNCB+5%PTN (5% PTN extract), for evaluating the change of appearance of skin surface, skin hydration, thickness of epidermis and mast cell numbers during 4 weeks. PTN suppressed symptoms of AD in appearance of skin and increased skin hydration for DNCB+1%PTN and DNCB+5%PTN. Treatment with PTN significantly decreased the levels of eosinophils. In histopathological examination, DNCB+1%PTN and DNCB+5%PTN significantly reduced the thickness of epidermis and number of mast cell in dermis. These results suggested that the PTN improved symptoms of AD in BALB/c mice.