• 제어로봇시스템학회논문지
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• 제9권10호
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• pp.844-851
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• 2003

We introduces a development result of semi-automatic karyotyping system using image processing method to improve a long time working of the manual method and 5% error of traditional automatic karyotying system for analyzing karyotying. The karyotyping procedures have many routine tasks such as searching metaphases, taking pictures, developing, editing, etc. There are several automatic karyotyping systems in order to reduce the task in advanced countries. However, they are very expensive, applicable to only human chromosome, and have too many functions to use easily. This paper takes aim at high quality image resolution and development of interface that can adjust brightness and contrast of image on-line. The system can be applied to animal and plants as well as human's chromosome. The system developed in this paper is applied to pig and human. The effectiveness of the system is proved by hospitals in Korea.

• Clinical and Experimental Reproductive Medicine
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• 제24권3호
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2003년도 동계 학술대회 논문집
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• pp.215-220
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• 2003

인간 게놈 프로젝트가 지속적으로 진행됨에 따라 계속적으로 대량의 유전체 정보가 밝혀지고 있으며, 이미 밝혀진 유전체의 염기서열을 바탕으로 다양한 생물의 전체 유전자의 기능을 효율적으로 해석하는 기술의 개발이 요구되고 있다. 식물 게놈 프로젝트 또한 식량확보라는 단순하면서도 전략적인 차원에서 가장 절실히 요구되는 기본 과학기술 연구분야이다. 게놈(genome)은 유전자(gene)와 염색체(chromosome)의 합성어로 한 생물체가 지닌 모든 유전 정보의 집합체이고, 동종의 재결합 DNA 분자를 포함하는 동일 세포의 개체를 클론(clone)이라 하며, 클론의 집합체를 콜로니(Colony)라 한다 생물체의 모든 유전정보를 가진 게놈은 핵산(nucleotide acid)이라 불리는 염기로 이루어져 있으며, 이들은 서로 상보적인 쌍을 이루어 두 가닥으로 형성되어 있다. 이를 한 쌍의 base pair라 한다. (중략)