• 제목/요약/키워드: Chromate

검색결과 147건 처리시간 0.023초

산화성 크롬의 배양세포에서의 독성작용 (Toxic Activities of the Oxidant Chromate in Culture Cells)

  • 박형숙
    • Environmental Analysis Health and Toxicology
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    • 제13권1_2호
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    • pp.1-9
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    • 1998

  • The ROS-producing potency of chromium compounds of several oxidation states were determined in the H4 cells. $K_2Cr_2O_7$ as Cr (VI), synthetic Cr (V) compounds and Cr (III) as TPP produced high level of ROS. However, ROS values of Cr-picolinate as Cr (III), CrCl$_2$, CrCI$_2$, were almost equal to the control. The effects of physiological antioxidants compounds which react with free radicals were examined for their effects on chromate-induced production of reactive oxygen species (ROS) in A549 cells after the addition of $K_2Cr_2O_7$. The compounds used were vitamin C (ascorbate), vitamin E ($\alpha$-tocopherol), superoxide dismutase (SOD) and catalase. The preincubation of ascorbate (200uM) with A549 cells for 20hr resulted in a significant reduction of hexavalent chromate(100uM) induced ROS. However, there is no effects of preincubation of the cells with vitamin E succinate (10 and 20uM, 20hr) on the ROS production. Also, the effects of Cr (VI) on the cell cycle of A549 cells was measured by adding the DNA intercalating agent, propidium iodide. S phase of the cell cycle was increased by the chromium (VI) compounds up to 20uM indicating toxicity or possible mitogenic action of the cell. The shoulder in Go/G1 phase at 20uM Cr (VI) with 24 hr treatment indicates apoptosis.

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연속배양 체제에서의 침출수 미생물에 의한 6가 크롬이온의 환원 (Reduction of Hexavalent Chromium by Leachate Microorganisms in a Continuous Suspended Growth Culture)

  • 김현영;오영숙;김영관;최성찬
    • 미생물학회지
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    • 제34권3호
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    • pp.126-131
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    • 1998

  • 침출수 미생물에 의한 크롬 6가이온의 3가이온으로의 환원을 회분식 배양 및 실험실 규모의 연속배양체제에서 연구하였다. 회분식 배양에 있어서 다양한 범위는 침출수 (10~60%)와 glucose (50~200 mM)를 첨가한 최소배지에서 초기 농도 $20mg\;L^{-1}$의 크롬(VI)을 첨가한 경우 72시간내에 최대 90%의 크롬(VI)이 침출수 미생물에 의하여 환원되었다. 이때 glucose의 첨가는 크롬환원을 촉진시켰으나, 배지내 침출수 비율의 증가는 오히려 크롬환원을 저해하였다. 후자의 경우는 침출수에 존재하는 황산이온이 크롬이온과 경쟁적 저해를 일으키기 때문인 것으로 추정되었다. 연속배양 실험은 124일간 상온에서 크롬(VI)의 농도를 $5{\sim}65mg\;L^{-1}$로 증가시키면서 수행하였으며, 수리학적 체류시간이 36시간일 때 $5{\sim}50mg\;L^{-1}$의 크롬농도 범위에서의 환원은 거의 100%에 달하는 것으로 나타났다. 평균 81.2%의 환원효율을 보인 101~124일 구간에서 계산된 specific reduction rate는 $3.492mg\;g^{-1}\;protein\;h^{-1}$였다. 이상의 결과는 침출수에 존재하는 미생물이 크롬은 함유한 다양한 침출수 또는 피혁폐수의 크롬 무독화과정에 유용하게 쓰일 수 있다는 가능성을 제시해 주는 것이다.

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Development of the Limit Switch Box for a Ship and Its Performance Evaluation against Salt Water

  • Lee, Seung-Heui;Go, Seok-Jo;Lee, Min-Cheol;Kim, Chang-Dong
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2005년도 ICCAS
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    • pp.1334-1338
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    • 2005

  • A limit switch box is used for an indicator of a valve actuator. This device indicates an opening and closing of a valve or a throttle in the valve actuator. In a ship, equipments require safety and robustness because of a rough environment and a specific condition during a voyage. However, the limit switch box has been used in an indoor environment generally. This study developed a new limit switch box which can be used at an outdoor environment. This study designed the new limit switch box. The housing of the limit switch box was made by an aluminum die cast method with surface painting after anodizing or chromate coating. In order to evaluate the endurance of the housing, the endurance tests against salt water have been conducted. Experiment results showed that the proposed device provides a reliable performance against salt water.

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무방향성 전기강판의 절연피막 코팅재 (Insulation Coating for non-oriented Silicon Steel Sheerts)

  • 조남웅;장세기
    • 한국표면공학회지
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    • 제30권6호
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    • pp.382-390
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    • 1997

  • Good appearance of insulation coating is required for non-oriented silicon steel sheets, The property is influenced by both the chemical composition of coating solution and the species of resin. The composition of inorganic-organic coating was studied to obtain good surface quality for non-oriented silicon steel. The greenish degree of coating surface depended on $Cr^{3+}$ content in the coating layer, which was satisfied when chromate content was more than 54.60 wt. %, in the coating solution. The homogeneous pattern and roughness of the coating surface depended on spreading property of the resin. Surface appearance of the coating could be improved by using resin with good spreading property at the chemical composition of chromate 59.00 wt.%.. resin 34.23wt.%, and etyhylene glycol 6.67 wt.% without colloidal sillica.

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