• Applied Biological Chemistry
• /
• 제36권3호
• /
• pp.208-214
• /
• 1993

엽록체 DNA 내에서 반복 염기서열의 존재와 이들에 의한 유전자 재배열 현상을 고찰하기 위하여 151bp Repeated Sequence 갖는 이질적인 유전인자군의 존재를 여러가지 품종의 벼 엽록체 DNA에서 관찰 하였다. 또한 쌀 DNA를 벼의 생장과 조직부위에 따라 분리하고, rp12 probe를 이용하여 Southern blot 분석하여 엽록체의 발달에 따르는 엽록체 DNA의 재배열 현상을 관찰하였다. 아울러 유전자 재배열 현상을 유발하는 반복염기서열을 database로부터 검색하여 유전자의 상호 비교 분석하였다. 그 결과 151bp Repeated Sequence와 유사한 염기 서열을 같는 rp123유전자를 포함하는 이질적인 유전인자군은 어느 특정한 품종의 벼에 국한되는것이 아니고 본 실험에 사용된 다양한 품종의 벼에 일반적으로 나타나는 현상임이 확인되었으며 또한 이들의 양상은 벼의 조직 부위에 따라 다르게 나타나고 있음을 확인하였다. 이러한 실험적 결과와 함께 엽록체 유전자 database의 검색과 유전자의 상호비교분석을 통하여 151bp 반복 염기 저열에 의한 벼 엽록체 DNA의 유전자 재배열현상은 식물 특히 단자엽 식물의 진화와 함께 발달된 현상으로 특히 151bp반복 염기 서열은 매우 다양한 유전자 재배열을 유발하는 변이유발 위치로 발달되어 왔음을 확인할 수 있었다. 따라서 이러한 반복염기서열에 의한 유전자 재배열 현상은 특히 벼에 있어서 plastid의 발달에 밀접하게 관여하고 있음을 제시하고 있다.

• BMB Reports
• /
• 제39권4호
• /
• pp.361-370
• /
• 2006

Genetic variation and molecular phylogeny of 22 taxa representing 14 extant species and 3 unidentified taxa of Boesenbergia in Thailand and four outgroup species (Cornukaempferia aurantiflora, Hedychium biflorum, Kaempferia parviflora, and Scaphochlamys rubescens) were examined by sequencing of 3 chloroplast (cp) DNA regions (matK, psbA-trnH and petA-psbJ). Low interspecific genetic divergence (0.25-1.74%) were observed in these investigated taxa. The 50% majority-rule consensus tree constructed from combined chloroplast DNA sequences allocated Boesenbergia in this study into 3 different groups. Using psbA-1F/psbA-3R primers, an insertion of 491 bp was observed in B. petiolata. Restriction analysis of the amplicon (380-410 bp) from the remaining species with Rsa I further differentiated Boesenbergia to 2 groupings; I (B. basispicata, B. longiflora, B. longipes, B. plicata, B. pulcherrima, B. tenuispicata, B. thorelii, B. xiphostachya, Boesenbergia sp.1 and Boesenbergia sp.3; phylogenetic clade A) that possesses a Rsa I restriction site and II (B. curtisii, B. regalis, B. rotunda and Boesenbergia sp.2; phylogenetic clade B and B. siamensis; phylogenetic clade C) that lacks a restriction site of Rsa I. Single nucleotide polymorphism (SNP) and indels found can be unambiguously applied to authenticate specie-origin of all investigated samples and revealed that Boesenbergia sp.1, Boesenbergia sp.2 and B. pulcherrima (Mahidol University, Kanchanaburi), B. cf. pulcherrima1 (Prachuap Khiri Khan) and B. cf. pulcherrima2 (Thong Pha Phum, Kanchanaburi) are B. plicata, B. rotunda and B. pulcherrima, respectively. In addition, molecular data also suggested that Boesenbergia sp.3 should be further differentiated from B. longiflora and regarded as a newly unidentified Boesenbergia species.

• 한국광과학회:학술대회논문집
• /
• 한국광과학회 1997년도 학술대회지
• /
• pp.39-39
• /
• 1997

• ALGAE
• /
• 제33권4호
• /
• pp.351-358
• /
• 2018

The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliable promoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed by protein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolae chloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknown aspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of useful chemicals.

• 한국산림과학회지
• /
• 제87권4호
• /
• pp.543-548
• /
• 1998

소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.

• Journal of Plant Biology
• /
• 제38권2호
• /
• pp.137-141
• /
• 1995

We isolated a cDNA clone, BLSC1, encoding 5' exon of ATP synthase CF0 subunit I from broccoli. BLSC1 is 285 nucleotides long which consists of a 5' noncoding region of 34 nucleotides, a 5' exon of 145 nucleotides and an intron of 106 nucleotides. The 5' exon codes for 48 amino acids which reveals mostly hydrophobic. The amino acid sequence deduced from BLSC1 shares 83%, 83% and 91% identities with the genes coding for atpF from wheat, rice and spinach, respectively. Genomic Southern blot analysis for BLSC1 showed a typically strong signal for a gene located in the chloroplast genome. Northern blot analysis identified three major classes of transcripts showing strong positive signals in the leaves, but only trace amounts of the transcripts were identified in the other organs like stems, flowr buds and roots.

• 미생물학회지
• /
• 제21권4호
• /
• pp.197-206
• /
• 1983

For the purpose of investigating the effect of nalidixic acid on the nucleic acid synthesis in chloroplast isolated from Chlorella ellipsoidea, cells were cultured in the media treated with nalidixic acid(20ppm) for 5 days. Aliquots cells were taken out at the inoculation and at intervals during the culture and growth rate of Chlorella cells measured. After extraction of nucleic acids in chloroplast isolated from these cells, their contents were analyzed by the base composition and the effect of nalidixic acid on the nucleic acid synthesis interpreted to compare with those of the control. 1. It was showed that the inhibitory concentration affected by nalidixic acid on the growth of Chlorella cells were 20ppm. 2. Because nalidixic acid had depressed the DNA replication in isolated chloroplast as well as whole cell system, these contents were markedly decreased in comparison with those of the control. 3. In the isolated chloroplast as well as in the whole cell system, nalidixic acid was decreased contents of base in the RNA by preventing RNA transcription.

• Journal of Plant Biology
• /
• 제35권4호
• /
• pp.415-423
• /
• 1992

Chlamydomonas reinhardtii 21 gr(mt+) strain의 배우체로부터 두 종류의 DNA methylase를 부분 분리하여 몇가지 기질 DNA에 대한 효소 활성을 측정하였다. DNA methylase I과 II는 동일한 pH와 ionic strength에서 서로 상이한 물리적인 성질과 서로 다른 분자량을 가지며 DNA methylase I과 II는 모두가 DNA 염기 중 adenine보다는 cytosine에 methylation을 수행하는 것으로 생각된다. 합성 DNA를 사용한 실험에서 DNA methylase I과는 달리 DNA methylase II는 poly(dA-dC)·poly(dG-dT)에서 보다 poly(dG-dC)·poly(dG-dC)의 oligonucleotide에서 더 높은 효소활성을 나타내었다. Chlamydomonas reinhardtii에서 추출한 엽록체 DNA를 기질로 사용하였을 때 DNA methylase I과 II 모두가 배우체기 보다는 영양생장기의 엽록체 DNA에 더 높은 활성을 나타내었다.

• 한국식물학회:학술대회논문집
• /
• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
• /
• pp.57-62
• /
• 1999

Light-regulated translation of chloroplast mRNAs requires nuclear-encoded trans-acting factors that interact with the 5' untranslated region (UTR) of these mRNAs. A set of four proteins (60, 55, 47, and 38 kDa) that bind to the 5'-UTR of the psbA mRNA had been identified in C. reinhardtii. 47 kDa protein (RB47) was found to encode a chloroplast poly (A)-binding protein (cPABP) that specifically binds to the 5'-UTR of the psbA mRNA, and essential for translation of this mRNA, cDNA encoding 60 kDa protein (RB60) was isolated, and the amino acid sequence of the encoded protein was highly homologous to plants and mammalian protein disulfide isomerases (PDI), normally found in the endoplasmic reticulum (ER). Immunoblot analysis of C. reinhardtii proteins showed that anti-PDI recognized a distinct protein of 56 kDa in whole cell extract, whereas anti-rRB60 detected a 60 kDa protein. The ER-PDI was not retained on heparin-agarose resin whereas RB60 was retained. In vitro translation products of the RB60 cDNA can be transported into C. reinhardtii chloroplast in vitro. Immunoblot analysis of isolated pea chloroplasts indicated that higher plant also possess a RB60 homolog. In vitro RNA-binding studies showed that RB60 modulates the binding of cPABP to the 5'-UTR of the psbA mRNA by reversibly changing the redox status of cPABP using redox potential or ADP-dependent phosphorylation. Site-directed mutagenesis of -CGHC- catalytic site in thioredoxin-like domain of RB60 is an unique PDI located in the chloroplast of C. reinhardtii, and suggest that the chloroplast PDI may have evolved to utilize the redox-regulated thioredoxin like domain as a mechanism for regulating the light-activated translation of the psbA mRNA.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
• /
• pp.230.1-230
• /
• 1996

No Abstract, See Full Text