• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1386-1391
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• 2017

The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen type I peptide (PIP) synthesis. A phenolic amide isolated from the stem extract was also assayed for these effects. The compound, N-trans-feruloyltyramine, was identified by $^1H$, $^{13}C$, and 2D-nuclear magnetic resonance analyses. In NO inhibition, the chloroform fraction (CF) exhibited the strongest inhibitory activity ($MIC_{50}=24.69{\mu}g/ml$) among the subfractions of the ethanol extract (EE). N-trans-feruloyltyramine isolated from the CF showed strong NO inhibitory activity, presenting with an $MIC_{50}$ of $31.36{\mu}g/ml$. The EE, CF, and N-trans-feruloyltyramine shown to have NO inhibition activity were assayed for the activity of PIP synthesis. The EE and CF showed relatively high PIP values of 38.8% and 24.21% at $100{\mu}g/ml$, respectively. The PIP value for $20{\mu}g/ml$ N-trans-feruloyltyramine showed a 36% increase compared with the non-treated control, whereas that treated with $20{\mu}g/ml$ ascorbic acid as a positive control showed a 13% increase. The results suggest that the proper stem extract of boxthorn stem could be efficiently used to produce good cosmetic effects.

• Preventive Nutrition and Food Science
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• 제18권1호
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• pp.76-79
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• 2013

We evaluated antioxidant activities of heated pear juice (HPJ) exposed to 120, 130, and $140^{\circ}C$ for 2 hr. HPJ was partitioned using n-hexane, chloroform, ethyl acetate, n-butanol, and water. The ethyl acetate fraction treated at $130^{\circ}C$ for 2 hr showed strong antioxidant activity; thus, this extract was isolated and purified using silica gel column chromatography and preparative high performance liquid chromatography. The structure of the purified compound was determined using ultraviolet and mass spectrometry, $^1H$-nucelar magnetic resonance (NMR), and $^{13}C$-NMR. Antioxidant activities of the isolated compound were evaluated and compared with ${\alpha}$-tocopherol, ascorbic acid, and butylated hydroxytoluene (BHT) using DPPH and ABTS assays. The isolated compound was identified as 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP). The DPPH radical-scavenging activity ($IC_{50}$) of DDMP occurred in the following order: ascorbic acid ($45.3{\mu}g/mL$) > ${\alpha}$-tocopherol ($69.2{\mu}g/mL$) > DDMP ($241.6{\mu}g/mL$) > BHT ($268.0{\mu}g/mL$). Furthermore, DDMP showed strong ABTS radical-scavenging activity (569.0 mg AA eq/g).

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.38-42
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• 2001

The methanol extracts of 25 leguminous seeds in vitro were evaluated for inhibitory activities against lens aldose reductase of Sprague Dawley male rats. The responses varied with both leguminous seed and concentration used. At the concentration of 0.1 mg/mL, the methanol extracts from Amphicaraea edgeworthii, Canavalia lineata, Gylcine max var. solitae, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. bangkong, Glycine max var. geumdu, Glycine max var. chungtae, Glycine max var. mejukong, Glycine soja, Phaseolus radiatus var. geodu, Vicia tetrasperma, Vigna angulasis, and Vigna sinensis inhibited enzyme activity by greatertha 60%. In following study, at the concentration of 0.01 mg/mL, the extracts of C. lineata and V. tetraspermahad relatively strong inhibitory activity against aldose reductase. Because of their potent inhibitory activities, the activity of each solvent fraction from C. lineata and V. tetrasperma was determined, and the potent activity was showed from chloroform and hexane fractions, respectively. {TEX}$IC_{50}${/TEX} values of C. lineata and V. tetrasperma were 0.004 and 0.006 mg/mL, respectively. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new agents of antidiabetic complications.

• 임산에너지
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• 24권2호
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• pp.16-23
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• 2005

삼지구엽초의 각 부분(잎, 줄기, 뿌리)을 MeOH로 추출한 후 농축하고 핵산, 클로로포름, 부탄올, 에틸아세테이트 및 수용성으로 분획하여 동결건조하였다. 각 추출물은 메탄을 수용액 및 에탄올-핵산 혼합액을 사용하여 Sephadex LH-20 칼럼크로마토그래피로 화합물을 분리 한 후 TBA 및 6% 초산에 전개하는 셀룰로오스 박층 크로마토그래피로 단리물질을 확인하였다. 단리물질의 화학적 구조는 $^1H-NMR,\;^{13}C-NMR$ 및 질량 분석으로 결정하였으며 단일화합물로 정제된 물질은 caffeic acid를 비롯하여 flavonoid와 그 배당체 화합물인 icariin, caffeic acid, hyperoside, Ikarisoside A, (+)-catechin이었다. 또한, 각 부분의 클로로포름 분획을 GC/MS (Libraries search)를 이용하여 정성분석을 실시하였다.

• 한국자원식물학회지
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• 제33권4호
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• pp.237-244
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• 2020

산수유 열수추출물의 혈전용해활성을 탐색하고, 유기용매로 분획화한 에틸아세테이트(ethyl acetate) 분획물에서 1.36 plasmin units/mL의 강한 혈전용해활성을 확인하였다. Amberlite IRA-400, Sephadex LH-20 column, active charcoal column과 재결정으로 정제한 혈전용해화합물을 HPLC로 분석 비교한 결과, 표준물질인 malic acid와 같은 retention time (RT)을 나타내고, LC/MS/MS 분석의 Negative mode 측정 시 m/z 133에서 같은 peak를 나타내어 정제한 화합물이 분자량 134 Da인 malic acid 임을 확인하였다. 정제한 화합물은 0.69 plasmin units/mL의 혈전용해활성을 보이고, 14.62%의 트롬빈 저해활성을 나타냈으며, 6.42%의 항산화 효과와 17.28%의 항당뇨 효과를 나타냈다. 분리한 화합물은 섬유소원의 Aα chain, Bβ chain과 반응하지 않았지만 γ chain을 분해하는 것으로 나타났다. 위 연구 결과를 바탕으로 산수유에서 분리한 혈전용해화합물은 혈관계 질환의 치료제 개발과 기능성 식품 개발을 위한 우수한 소재로 이용 가능할 것으로 예상된다.

• The Plant Pathology Journal
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• 제15권3호
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• pp.152-157
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• 1999

An antibiotic compound was isolated from the culture of an antagonist against Fusarium oxysporum f. sp. sesami, Bacillus polymyxa strain KB-8, and tested for the control of Fusarium wilt of sesame in greenhouse conditions. Optimum conditions for culturing the antagonist to obtain the maximum antibiotic activity were determined using different culture media, initial medium acidity, and incubation periods for which yeast -malt extract agar with the initial acidity of pH 5 and over 13 days culture were best. Antibiotic substances extracted by methanol had 2 main fractions, KB-8A and KB-8B, in thin layer chromatography (OLC) with Rf values of 0.35 and 0.67 in a solvent system of chloroform : methanol = 7 : 3. The fraction KB-8A wa purified further by XAD-2, silica gel and Sephadex LH-20 column chromatography, and crystalization. Its minimum inhibitory concentrations (MICs) were $12.8\mu\textrm{g}$/ml for F. oxysporum and Alternaria mali, $6.4\mu\textrm{g}$/ml for Colletotrichum gloeosporioides and Rhizoctonia solani, and $3.2\mu\textrm{g}$/ml for Phytophthora capsici. Soil drenching of antibiotic KB-8A in the concentrations of $13.0\mu\textrm{g}$/ml and $26.0\mu\textrm{g}$/ml effectively inhibited the Fusarium wilt of sesame in a greenhouse test, which appeared to be comparable to the fungicide benlate of $6.5\mu\textrm{g}$ a. i./ml.

• 한국지하수토양환경학회지:지하수토양환경
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• 제16권5호
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• pp.42-52
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• 2011

In this laboratory study, the changes in gas-exposed perchloroethene (PCE) saturation in sand during a PCE removal process were measured using gaseous tracers. The flux of fresh air through a glass column packed with PCEcontaminated, partially water-saturated sand drove the removal of PCE from the column. During the removal of PCE, methane, n-pentane, difluoromethane and chloroform were used as the non-reactive, PCE-partitioning, water-partitioning, and PCE and water-partitioning tracers, respectively. N-pentane was used to detect the PCE fraction exposed to the mobile gas. At water saturation of 0.11, only 65% of the PCE was found to be exposed to the mobile gas prior to the removal of PCE, as calculated from the n-pentane retardation factor. More PCE than that detected by n-pentane was depleted from the column due to volatilization through the aqueous phase. However, the ratio of gas-exposed to total PCE decreased on the removal of PCE, implying gas-exposed PCE was preferentially removed by vaporization. These results suggest that the water-insoluble, PCE-partitioning tracer (n-pentane in this study), along with other tracers, can be used to investigate the changes in fluid (including nonaqueous phase liquid) saturation and the removal mechanism during the remediation process.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.2235-2245
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• 2016

Ulcerative colitis (UC) results from colonic epithelial barrier defects and impaired mucosal immune responses. In this study, we aimed to investigate the modifying effects of a Spirogyra neglecta extract (SNE), a polysaccharide extract (PE) and a chloroform fraction (CF) on dextran sodium sulfate (DSS)-induced colitis in mice and to determine the mechanisms. To induce colitis, ICR mice received 3% DSS in their drinking water for 7 days. Seven days preceding the DSS treatment, oral administration of SNE, PE and CF at doses of 50, 25 and 0.25 mg/kg body weight (low dose), 200, 100 and 1 mg/kg body weight (high dose) and vehicle was started and continued for 14 days. Histologic findings showed that DSS-induced damage of colonic epithelial structure and inflammation was attenuated in mice pre-treated with SNE, PE and CF. Furthermore, SNE and PE significantly protected colonic epithelial cells from DSS-induced cell cycle arrest, while SNE, PE and CF significantly diminished apoptosis. Proteome analysis demonstrated that SNE and PE might ameliorate DSS-induced colitis by inducing antioxidant enzymes, restoring impaired mitochondria function, and regulating inflammatory cytokines, proliferation and apoptosis. These results suggest that SNE and PE could prevent DSS-induced colitis in ICR mice by protection against and/or aiding recovery from damage to the colonic epithelium, reducing ROS and maintaining normal mitochondrial function and apoptosis.

• 한국식품과학회지
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• 제39권4호
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• pp.458-463
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• 2007

식품소재의 단백질 glycation 저해효과를 조사하기 위하여 곡류, 채소류 및 생약재 등 83종 에탄올 추출물에 대한 단백질 가교결합 저해효과를 $[^{14}C]$-N-formyl-lysine incorporation method를 이용하여 측정하였다. 대부분의 추출물에서 저해효과가 나타났으나 일부 추출물은 단백질의 가교를 촉진하는 것으로 나타났다. 상대적으로 높은 활성을 나타낸 시료 중 20개를 선별하여 형광광도법을 이용한 AGE형성 억제능을 조사한 결과, 1일 발아한 메밀의 추출물 (GB-l)이 가장 높은 저해활성을 가지는 것으로 나타났다. 차후 GB-0l은 콜로로포름, 에틸아세테이트, 부탄올 및 물로 분획되었으며 그 중 에틸아세테이트 분획이 가장 높은 단백질 glycation 저해활성(95.2% inhibitory activity at 100 ug/mL addi- tion level)을 보였다. HPLC를 이용한 폴리페놀 분석결과 발아 및 추출, 분획과정을 거친 메밀의 아세테이트 분획은 rutin 및 quercetin 함량이 상당히 증가한 것으로 나타나서 부분정제물의 rutin 및 quercetin 함량과 저해활성사이에 높은 상관성을 나타내었다.

• 동의생리병리학회지
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• 제20권3호
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• pp.617-622
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• 2006

In the present study, we investigated the anticariogenic compounds from Aralia continertalis (A. continnentalis) has been isolated and identified by MS, $^1H-NMR$ and $^{13}C-NMR$. The MeOH extract was suspended in $H_2O$ and sequentially Partitioned with $CHCl_3$, EtOAc, and n-BuOH. The $CHCl_3$ fraction showed remarkable antibacterial activity against S. mutans. The antibacterial activity compounds against 5. mutans by MIC was isolated successively through the screening system and various chromatography methods. From this active chloroform subfraction, isolation and identification finally gave continentalic acid by spectroscopic methods (MS, $^1H-NMR$ and $^{13}C-NMR$) as an active principle. The compound, continentalic acid, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against 5. mutars. These results suggest that continentalic acid from A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.