• Title/Summary/Keyword: Chlorobium vibrioforme

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Detection of Bacteriochlorophyll-c Containing Species of Green Sulfur Photosynthetic Bacterium Chlorobium vibrioforme

  • Yoshitaka Saga;oka, Hirozo-Oh;Hitoshi Tamiaki
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.341-343
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    • 2002
  • Bacteriochlorophyll(BChl)-c containing species of green sulfur photosynthetic bacterium Chlorobium (ChI.) vibrioforme, which has BChl-d mainly, was detected. We obtained colonies on agar plates by spreading the liquid culture of ChI. vibrioforme f. sp. thiosulfatophilum strain NCIB 8327 which contained the high ratio of BChl-c/BChl-d, and transferred each colony into a new liquid medium. These cultures after growing were found to be classified into two categories. One possessed BChl-d as a light-harvesting pigment and the other did BChl-c. No colonies examined here contained both BChls-d and c. Therefore, the presence of both BChls-d and c in our cultures of ChI. vibrioforme was ascribed to the coexistence of two different cells which had BChl-d and c as the chlorosomal pigment, respectively. The change of pigment composition observed in our liquid cultures can be thus explained by the difference of growth rates between two kinds of cells.

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Isolation and Structural Determination of a Complete Set of Intact Bacteriochlorophyll-d Homologs and Isomers from Green Sulfur Bacterium Chlorobium vibrioforme and Their Aggregation Properties in Hydrophobic Solvents

  • Mizoguchi, Tadashi;Saga, Yoshitaka;Tamiaki, Hitoshi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.344-346
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    • 2002
  • Eight intact bacteriochlorophyll (BChl)-d homologs and isomers were isolated from a strain of green sulfur bacterium Chlorobium vibrioforme. All the molecular structures of the BChl-d components were fully determined by a combination of mass spectrometry and $^1$H-NMR spectroscopy. The aggregation behavior of the isomerically pure BChls-d in hydrophobic organic solvents was examined with respect to the stereoisomeric configuration at the C3$^1$ position as well as the bulkiness of the C8 and C12 side-chains by using electronic- absorption spectroscopy.

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