• 한국미생물·생명공학회지
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• 제14권6호
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• pp.433-439
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• 1986

Chlamydia trachomatis는 인간에게 각종 질병을 일으키는 병원균이며 최근 그 중요성 이 크게 증가하였다. Chlamydia trachomatis감염에 대한 재래식 진단방법은 낮은 정밀도와 낮은 특이성을 갖고 있으며 방법의 수행 자체도 어렵다는 문제점을 지니고 있다. 이들 재래식 진단방법들이 안고 있는 문제점들을 해결하기 위하여 항-병원균 모노클로날 항체를 생산하는 하이브리도마 세포주를 만들 최종목표를 갖고 우선 생쥐에 면역시킬 항원으로 사용할 Chlamydia trachomatis를 동물 숙주 세포내에서 증식시키는데 끼치는 각종 인자들에 대한 영향을 조사연구하였다. 동물 세포 유래의 McCoy 세포내에서 충분한 양의 Chlamydia tracthomatis를 증식시킨 후 불연속 Urografin 구배 원심분리에 의해 정제하였다. Mc-Coy 세포 내에서의 Chlamydia trachomatis의 생성 개체수를 늘리기 위하여 화학제제를 사용했던 바 IUdR 처리가 cycloheximide 처리보다 더 효과적이었으며 Chlamydia trachomatis 증식을 위해 쓸 수 있는 방법이었다. 원심력의 증가가 Chlamydia tracho chomatis의 McCoy 세포 표면에의 흡착을 증진시켰으며 약 3000g 근처에서 최대 감염율을 보였다. 분리 정제한 Chlamydia trachomatis는 SPG 용액 내에서 4$^{\circ}C$에서 48시간 동안 저장될 수 있었으며 더욱 오래 동안 저장시키기 위해서는 -7$0^{\circ}C$까지 냉동시키는 것이 필요하였다.

• 대한수의학회지
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• 제46권1호
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• pp.13-19
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• 2006

Generally known psittacosis or ornithosis is a disease of birds caused by the bacterium Chlamydia psittaci. Humans are accidential hosts and are most commonly infected from avian sources. It raises hepatitis or neurosis. As major outer membrane protein (MOMP) of Chlamydia psittaci has been known to play a role in the avoidance of host immune defenses, research on developing a Chlamydia vaccine has focused on the MOMP. In this study, the gene encoding the major outer membrane protein (MOMP) of the Chlamydia psittaci strain 6BC was cloned and expressed in Escherichia coli strain M-15. The recombinant DNA was cloned by fusion prokaryotic expression vector pQE30-GFPII. Expression of the recombinant protein was performed in E. coli and was induced by IPTG. The size of expressed recombinant protein is 74.220 kDa (MOMP, 43.260 kDa; GFP expression region, 30 kDa; $6{\times}His$ tag, 960Da). This protein was purified by using his-tagging-inclusion body. Recombinant protein was reconfirmed through ELISA test and western blot with antibody against pQE30-GFPII. It will be useful antibody development.

• Clinical and Experimental Pediatrics
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• 제52권3호
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• pp.277-282
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• 2009

Pneumonia, which usually requires hospitalization for children, is caused by various pathogens. According to recent surveys, the prevalence of atypical pneumonia caused by Mycoplasma or Chlamydia has increased, especially in preschool children. Also, the evidence has been accumulated that Mycoplasma or Chlamydia infection is associated with asthma including both inception and exacerbation. Therefore, it is important to consider how the clinical aspects of Mycoplasma pneumonia have changed. In particular, Chlamydia pneumonia, which has not been prevalent in Korea, needs greater attention.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.790-798
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• 2016

Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection.

• Journal of Yeungnam Medical Science
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• 제6권2호
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• pp.23-28
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• 1989

1989년 6월 1일부터 8월 31일까지 안분비물을 주소로 본원 외래를 방문한 신생아 및 입원중인 신생아를 대상으로 Giemsa염색 및 Chlamydiazyme 검사, Gram염색 및 세균 배양검사를 실시하여 다음과 같은 결론을 얻었다. 1. Chlamydia성 결막염으로 진단된 환자는 전체 대상 환아의 34.6%였다. 2. Chlamydia성 결막염 발생시 일령 은 6일에서 15일사이가 가장 많았으며 남녀 성별차이는 없었다. 3. Chlamydia성 결막염으로 진단된 환아는 임상 증상 및 이학적 소견만으로 다른 세균성 결막염과 구분할 수 없었다. 4. 안분비물의 세균배양시 아무균도 자라지 않았던 경우가 61.5%였으며 균주가 자란 경우는 Pseudomonas aeruginosa가 19.2%로 가장 많았으며 Chlamydia성 결막염으로 진단된 경우도 1례에서도 S. aureus가 배양되었다. 5. Chlamydia성 결막염으로 진단된 환아 중 폐렴이 동반된 례는 없었다.

• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1109-1114
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• 2021

Chlamydia pneumoniae is a type of pathogenic gram-negative bacteria that causes various respiratory tract infections including asthma. Chlamydia species infect humans and cause respiratory infection by rupturing the lining of the respiratory which includes the throat, lungs and windpipe. Meanwhile, the function of interleukin-4 (IL-4) in Ch. pneumoniae respiratory infection and its association with the development of airway hyperresponsiveness (AHR) in adulthood and causing allergic airway disease (AAD) are not understood properly. We therefore investigated the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. In this study, Ch. pneumonia strain was propagated and cultured in HEp-2 cells according to standard protocol and infant C57BL/6 mice around 3-4 weeks old were infected to study the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. We observed that IL-4 is linked with Chlamydia respiratory infection and its absence lowers respiratory infection. IL-4R α2 is also responsible for controlling the IL-4 signaling pathway and averts the progression of infection and inflammation. Furthermore, the IL-4 signaling pathway also influences infection-induced AHR and aids in increasing AAD severity. STAT6 also promotes respiratory infection caused by Ch. pneumoniae and further enhanced its downstream process. Our study concluded that IL-4 is a potential target for preventing infection-induced AHR and severe asthma.

• 대한미생물학회지
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• 제21권3호
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• pp.393-397
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• 1986

Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamydia trachomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. We have studied a sensitivities of culture, chlamydiazyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2'nd sample was used to Chlamydiazyme test and 3'rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%). 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlamydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyme and DIF negative cases are 43/85(50.1%). In summarized, anyone positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%).

• Journal of Microbiology
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• 제41권2호
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• pp.137-143
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• 2003

The present study was performed to identify pathogenic factors of Chlamydia trachomatis, which invade the host cell membrane. We prepared monoclonal antibody against C. trachomatis and searched for pathogenic factors using this antibody, and subsequently identified the surface components of the elementary body of C. trachomatis, i.e., major outer membrane protein (MOMP), lipopolysaccharide (LPS), and two other surface exposure proteins. These proteins are believed to be important in the pathogenesis of host cell chlamydial infection. Additionally, to identify factors related to the host cell and C. trachomatis, we prepared C. trachomatis infected and non-infected McCoy cell extracts, and reacted these with anti-chlamydial LPS monoclonal antibody. We found that anti-chlamydial LPS monoclonal antibody reacted with a 116 kDa proteinaceous McCoy cell membrane component.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1497-1500
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• 2014

Cervical cancer continues to be an important public health problem in Thailand. While the high risk human papillomavirus (HPV) types have been established as the principle causative agent of both malignancies and the precursor lesions, cervical intraepithelial neoplasia (CIN), other factors may also be involved like other sexually transmitted diseases, as well as smoking. Chlamydia trachomatis is an obligate intracellular Gramnegative bacterium which has a tendency to cause chronic infection featuring inflammation and therefore might be expected to increase the risk of cervical cancer. In the present nested case-control study, 61 cases of cervical cancer and 288 matched controls with original serum samples were identified from the Khon Kaen Cohort, established in the North-East of Thailand, by linkage to the Khon Kaen population based cancer registry. C. trachomatis specific IgG antibodies at recruitment were measured by microimmunofluorescence and assessed for association with cervical cancer using STATA release10. No significant link was noted either with all cancers or after removal of adenocarcinomas. The results suggest no association between Chlamydia infection and cervical cancer development in North-East Thailand, but possible influencing factors must be considered in any future research on this topic.

• Tuberculosis and Respiratory Diseases
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• 제59권1호
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• pp.47-52
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• 2005

연구배경 : 클라미디아 폐렴균은 임상적으로 중요한 균으로 혈청검사가 진단에 중요하다. 현재 이용되는 micro-immunofluorescence법은 주관적이고 시간이 많이 소요되므로 좀더 객관적으로 진단할 수 있는 ELISA법을 이용하여 만성 기침을 주소로 내원한 환자들의 혈청을 대상으로 검사하고 표준방법과 결과를 비교하였다. 방 법 : 2003년 8월부터 2004년 7월까지 강원대학교병원을 방문한 35명의 성인 환자에서 얻은 혈청을 ELISA법과 micoimmunofluorescence법으로 시행하였다. 비인두 스왑에서 PCR을 시행하였다. 두 방법의 비교는 비모수 상관계수 분석(Spearman)을 하였다. 결 과 : ELISA 검사결과 IgG는 민감도 84.0%, 특이도 60.0%, IgA는 민감도 84.0%, 특이도60.0%, IgM은 민감도 40.0%, 특이도 96.7%의 결과를 보였다. PCR은 3명의 환자에서 양성이었다. 결 론 : ELISA법이 많은 환자들을 대상으로 하는 역학적 연구에서는 좀더 유용하게 이용될 수 있을 것으로 사료된다. 임상적으로 적용되기 위해서는 좀더 연구가 필요할 것으로 사료된다.