• Animal cells and systems
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• 제15권4호
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• pp.310-314
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• 2011

The Banna miniature pig (BNMP) is a representative miniature pig breed in China. Even though BNMP dwarfism is obvious, its underlying causative mutations remain unknown. In this study, the BNMP and Large White pig (LWP) serum growth hormone (GH) and insulin-like growth factor (IGF-1) levels were detected by ELISA and compared. BNMP serum IGF-1 levels were significantly lower than LWP levels (P<0.05). The miniature condition may arise from mutations in the GH and GH receptor (GHR) genes. Therefore, GH and GHR cDNA from the BNMP were cloned into a pMD18-T vector by RT-PCR using the total RNA obtained from the BNMP's pituitary and liver tissues. Sequencing results indicated that the open reading frame of the BNMP GH gene is composed of a 26-residue signal peptide and a 191-residue mature peptide. The coding sequence of the BNMP GHR gene contained 639 amino acids, including a signal peptide that is 18 amino acids long. Two amino acid substitutions, A09V and R22Q, were found in the signal peptide of the GH gene. Additionally, the S104P mutation was found in the BNMP's mature GH protein. Four mutations in the cytoplasmic domain of GHR may influence the downstream signal transduction of GHR, which needs further experimental evidence.

• 한국수정란이식학회지
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• 제13권2호
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• pp.159-172
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• 1998

사람 성장호르몬 수용체(hGHR) cDNA는 PCR방법에 의하여 fagment로서 보고되어진 바 있으나, liver cDNA로 부터 전장을 cloning한 보고는 없는 실정으로 본 연구에서는 기능을 가진 약 4.6kbp의 cDNA hGHR을 cloning 하는데 성공하였다. 먼저 cloning하기 위하여 human liver mRNA와 human breast cancer tissue로부터 회수한 mRNA를 RT-PCR방법에 의하여 human cDNA library와 cloning에 필요한 probe를 제작하였다. human library mRNA는 GT-PCR방법에 의하여 증폭하여 증폭되어진 산물은 λZAP Vector를 이용하여 cDNA library를 구축하였고,screeing을 위하여 임 보고 되어진 hGHR fragment native sequence를 기초로 N-terminal부분의 primer를 설계하여 950bp의 probe를 얻는데 성공하였다. 이 probe를 이용하여 준비된 human liver cDNA library로부터 2.5$\times$10 6개의 plaque로부터 6개의 positive clone을 획득하였고, 이들중 poly Asignal인 "AATAAA"를 포함하고 있는 가장 긴 약 3.8kbp의 clone을 sequencing한 결과 open reading frame을 포함하고 있었으나, 5'부분의 결손되어 있었다. 그리하여 이 부분은 human breast cancer tissue로 부터 회수한 mRNA를 RT-PCR에 의하여 증폭하였고, sequencing결과 이미 보고되어진 native hGHR와 비교한 결과 하나의 nucleotide가 silent mutation으로 판명되었다.한편 human liver cDNA library로부터 cloning한 3.8cp의 positive clone의 5'end의 결손된 부분에 silent mutation된 PCR 산물을 연결함으로써 native hGHR와 유사한 cDNA hGHR subcloning에 성공하였다. 이러한 cDNA hGHR의 clone이 function을 가지고 있는지를 검토하기 위하여 eukaryotic 발현 vector인 pCXN2에 의거 ligation한 후 chinese hamster ovary cell[CHO-KI]에 transfect를 실시하였다. Dexamethasone은 첨가하지 않고 hGH만의 존재하에서 이들 cell을 배양시키고 cell menbrane에서 발현 여부를 판정키 위하여 hGHR monocloual antibody를 사용하여 flow cytometery해석을 실시하는 한편 125I-hGH binding assay에 의하여 hGH binding activity를 측정하였다. 최종적으로 GH signal transduction의 target genedf으로 알려져 있는 serine protease inhibitor 2.1(Spi 2.1) gene의 promotor activity를 검토한 결과 hGHR을 transfect한 CHO Cell에 있어서 hGH의 농도에 의존적으로 증가되었다. 따라서 본 실험에서 cloning한 cDNA hGHR는 native hGHR와 같은 기능을 가지는 것으로 판명되었다.것으로 판명되었다.

• The Plant Pathology Journal
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• 제24권4호
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• pp.478-481
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• 2008

The cDNA clone of Brassica rapa WRKY7 (BrWRKY7) was obtained from EST collection in Brassica genomics team and its DNA sequence was determined. The cDNA clone is 1,037 bp long in nucleotides and encodes an open reading frame of 307 amino acids. Based on a phylogenetic tree, BrWRKY7 belongs to group IId. BrWRKY7 was induced by wound and SA. It was also induced by pathogen attack such as Xanthomonas campestris pv. campestris (Xcc), suggesting that this BrWRKY may play an essential role in defense response of chinese cabbages.

• 대한한의학원전학회지
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• 제30권3호
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• pp.11-40
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• 2017

Purpose : The purpose of this article is to make a clarification that Hwang Doyeon(黃度淵) was a official doctor(醫官) who even served as royal doctor(御醫), rather than merely a general doctor who worked in the private sector. The article also attempts to give a new perspective on the medical history about the late period of Joseon(朝鮮) Dynasty. Methods : In researching Hwang Doyeon's master work, the Gains and Losses of Medical Orthodoxy(醫宗損益), the article looks at his clinical medicines. Analyzing the historical records, the article makes an assumption that Hwang Doyeon and Hwang Dosoon(黃道淳), who was selected as an officer to discuss medicine with other medical officers(議藥同參), are the identical person. On this assumption, the article tries to reinterpret the medicine during the later Joseon Dynasty period. Result & Conclusion : The records, including the royal medical treatment records of the Gains and Losses of Medical Orthodoxy, the Daily Records of Royal Secretariat(承政院日記), the genealogy of Changwon Hwangs(昌原黃氏世譜), academic paper and government documents released posthumously, demonstrated that Hwang Doyeon and Hwang Dosoon are the same person. If so, we can have new historical interpretation about medical history of late Joseon Dynasty. First, during the late of Joseon Dynasty, there was an official and active medical knowledge interaction between government and private sector. Second, the Joseon's medicine has been established its own medical system based on the Treasured Mirror of Eastern Medicine(東醫寶鑑), trying to cope with cholera and other contemporary epidemics without relying on Chinese warm disease(溫病) medicine. Third, the Compilation of Formulas and Medicinals(方藥合編), is regarded not only as a must-read medical reading for medical doctors, also playing an important role on popularization of Korean medical knowledge.

• 혜화의학회지
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• 제20권1호
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• pp.25-37
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• 2011

It fuses into culture of the East and the West in use of color ; colors of five direction, which had been from the Five Phase Theory in East Asia. Formative ability of color fields in a formative early Oriental Medicine history as well. Color is a very important factor and a necessary step of visible diagnosis. As a human's body is maintained and alive under the control of spirit and spirit is stored by five viscera, so spirit expresses the change of five viscera and is reflected by color. Visible diagnosis consists of spirit, appearance, Qi and color and movement. The purpose of each visible diagnosis is that we would know states of essence, Qi and spirit in patient's body. To ancient Asia people 'to see' was a kind of insight to the object as a whole. Similarily the activity of seeing the human body was the integral part of making diagnosis of a patient. This was the cause that there was suggested the discussion of the Five color theory in Oriental Medicine as a counterpart to that of the 'Goethe for Zur Farbenlehrer'. The inspection of Oriental Medicine was not a simple gazing of the eye as a sense organ, but the total insight to the internal state of the patient. For that reason, the eye－perception in early Chinese medicine was the reading the signs of the internal body which had have not the visual form but the flux of the internal life.

• BMB Reports
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• 제40권3호
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• pp.358-367
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• 2007

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

• 혜화의학회지
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• 제19권2호
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• pp.25-33
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• 2011

Daoism is a very important subject that consists of oriental medicine(traditional east asia medicine). Among the many scriptures, The Zhouyi cantong qi (周易參同契, Token for Joining the Three in Accordance with the Book of Changes) is the main Chinese alchemical scripture. This book is composed with three kinds of subject, Zhouyi(周易, the Book of Changes), the Huanglao(黃老) Tradition and alchemy(鍊金, 爐火). The author's name is not signed but is concealed in the text. According to the traditional account, the legendary Han immortal from Guiji (會稽, in present-day Zhejiang, 古 浙江), Wei Boyang(魏伯陽), wrote it in the period between Emperor Shun and Emperor Huan of the Eastern Han (126-127 BC), after reading the Longhu jing (龍虎經, Scripture of the Dragon and Tiger). Later he transmitted it to Xu Congshi(徐從事), who appended a commentary, and to Chunyu Shutong(淳于叔通), who first circulated it in the world. While some features of this account provide significant details - especially about the reputed date of the text and about its formation having taken place in stages - the received Cantong qi(參同契) actually is not the product of a single generation of authors, but the result of several centuries of textual accretions as well as theory of three co-authorship by Wei Boyang(魏伯陽), Xu Congshi(徐從事), Chunyu Shutong(淳于叔通). It has over 6000 characters in four-word or five-word verses. Some parts of the book are in styles of prose and poem. Many scholars explain the title "cantongqi(參同契)", saying that "Can(參)" means three, "Tong(同)" means correspondence, and "Qi(契)" means unification. Through images of hexagrams of the Book of Changes, the book illustrates the thought of the Huanglao(黃老) Tradition and alchemy. Wei Boyang(魏伯陽) theorizes his own experience unifying the way of intercourse of Yin and Yang in the Book of Changes, the cultivation of spirit through spontaneity of the Huanglao(黃老) Tradition and the elixir refining of alchemy.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• 보존과학회지
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• 제32권1호
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• pp.11-19
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• 2016

승자총통은 중국 화기를 모방하여 선조(宣祖,1552-1608) 시기에 독자적인 화기 제작방식으로 만들어진 우리나라 유통식 소화기(小火器)이다. 이 연구는 경희대학교 중앙박물관 소장 승자총통을 중심으로 명문 판독 결과와 함께 과학분석 결과를 정리한 것이다. 경희대학교 중앙박물관에 소장하고 있는 승자총통 3점은 각 원소의 함량차이가 크지 않은 Cu-Sn-Pb 삼원계 합금으로 제작되었고 분석대상 중 미세조직 분석이 가능한 승자총통 1의 미세조직을 분석한 결과 일반적인 주조방식에 의해 제작되었으며 추가적인 열처리나 담금질 흔적은 관찰되지 않는다는 점이 밝혀졌다. 또한 승자총통 제작 시 사용한 납의 출처를 알아보고자 납동위원소비 분석을 실시한 결과 한국 남부 zone 2 지역(경상북도 북부와 강원도 남부지역)의 납광석을 사용하여 승자총통을 제작한 것으로 판단된다.

• 아동학회지
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• 제6권2호
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• pp.21-33
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• 1985

This study was conducted to ascertain whether a didactic mode of instruction, favored students who indicated a preference for left hemispheric information processing. The subjects were 50 fifth grade students whose California Achievement Test score percentile ranks were between 50 - 80. They were randomly selected from one elementary school in Chapel Hill, NC, USA. All Ss completed the Form CC of "Your Style of Learning and Thinking" (Torrance and McCarthy, 1980) which is a paper/pencil inventory designed to categorize the subjects according to their hemispheric preference for processing information; right hemisphere, left hemisphere, and integrated. Then all Ss received a didactic mode of instruction, a verbal question/answer followed by teacher's reading of a fictional animal story, "The Chinese Bee-eater". After completion of instruction, the Ss were asked to respond to eighteen open-ended questions about the animal. Eight of the 50 subjects were found to fall into the right hemisphere style of information processing group; fourteen into left; and fourteen into integrated. The remaining fourteen could not be assigned to either of these three categories and were not included in the analysis of the results. the mean scores of the comprehensive test among hemispheric groups and among sex groups. A two way analysis of variance also presented no significant differences among the hemispheric preference combined by sex groups. These results failed to prove the research hypothesis that students who indicate a preference for left hemispheric information processing will have higher scores on a comprehensive test after receiving a didactic mode of instruction, the so-called left style of instruction. Some limits in research process and conceptual confusion about hemisphericity were discussed. Finally, it was suggested that educators need to be more careful in drawing educational implications from neurological hemispheric research.