• Journal of Ginseng Research
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• 제47권4호
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• pp.524-533
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• 2023

Background: Obesity is a risk factor for aging and many diseases, and the disorder of lipid metabolism makes it prominent. This study aims to investigate the effect of ginsenoside Rg1 on aging, lipid metabolism and stress resistance Methods: Rg1 was administered to Caenorhabditis elegans (C. elegans) cultured in NGM or GNGM. The lifespan, locomotory activity, lipid accumulation, cold and heat stress resistance and related mRNA expression of the worms were examined. Gene knockout mutants were used to clarify the effect on lipid metabolism of Rg1. GFP-binding mutants were used to observe the changes in protein expression Results: We reported that Rg1 reduced lipid accumulation and improved stress resistance in C. elegans. Rg1 significantly reduced the expression of fatty acid synthesis-related genes and lipid metabolism-related genes in C. elegans. However, Rg1 did not affect the fat storage in fat-5/fat-6 double mutant or nhr-49 mutant. Combined with network pharmacology, we clarified the possible pathways and targets of Rg1 in lipid metabolism. In addition, Rg1-treated C. elegans showed a higher expression of anti-oxidative genes and heat shock proteins, which might contribute to stress resistance Conclusion: Rg1 reduced fat accumulation by regulating lipid metabolism via nhr-49 and enhanced stress resistance by its antioxidant effect in C. elegans.

• 대한추나의학회지
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• 제1권1호
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• pp.103-117
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• 2000

The manipulations are the main therapheutic procedure of Chuna. Manipulation is the essential procedure of Chuna theraphy. On the basis of touch parts and motion changes, each elementary manipulation is divided into Stimulating Manipulations (reflexional manipulations) and Mobilizing Manipulations. Objectives: This study were advanced so as to know Chinese Chuna Manipulations especially on Herniated Nucleus Pulposus. Methods We Investigated Chinese medicine paper about Chinese Chuna Manipulations on Herniated Nucleus Pulposus between 1996 and 1999. Results. Chinese Chuna theraphy, Pressing Manipulations and Thrusting-Wrenching Manipulations are mostly used on Herniated Nucleus Pulposus, and Chinese Chuna theraphy carrys out Traction, Injection. Chinese medicine. Acupuncture side by side. Conclusions: Chinese Chuna theraphy uses not only Mobilizing Manipulations, but also Stimulating Manipulations whereas Korean Chuna theraphy chiefly uses Mobilizing Manipulations.

• 대한한의진단학회지
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• 제12권1호
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• pp.1-12
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• 2008

Object: The purpose of this study is to the review recent 20 years chinese achievements on the use of impedance cardiography for chinese medicine. Methods: From the China Journal Full Text Database we searched papers on the use of impedance cardiography for chinese medicine by the keyword "심조항도(心阻抗圖)". Results: The researches are summarized into 3 parts. (1) the researches on the relationship between the parameters of impedance cardiography and the chinese medical diagnoses; pulse diagnosis and tongue inspection. (2) the researches on the objective assessment using impedance cardiography for the effects of chinese medical therapy. (3) the researches on the objective evaluation index of Heart Deficiency Syndrome using impedance cardiography. Conclusion: The parameters of impedance cardiography has been established for its usefulness in various chinese medical researches.

• 동의생리병리학회지
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• 제24권3호
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• pp.373-379
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• 2010

There has been a need for establishing operational curriculum for chinese characters and chinese writing used by traditional korean medicine (TKM), but it was not carefully recognized so far. We analysed the frequency of unicode chinese characters from five medical textbooks and showed prerequisite chinese characters for TKM beginners. It was found that 之, 者, 不, 也, 而, 氣, 陽, 陰, 下, 其, 病, 爲, 人, 以, 中, 則, 於, 脈, 上, 故 are the most frequently used 20 chinese characters. We also showed that adequate prerequisite chinese character should be designated for the more efficient education of TKM. This study was the first systematic approach to get essential and prerequisite chinese characters for the education of TKM. The prerequisite characters by this study will be used for the development of KEET (Korean Medicine Education Eligibility Test), entrance exam to the Colleges of Oriental Medicine and textbooks, and educational curriculum of premed students.

• Journal of Ginseng Research
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• 제46권6호
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• pp.750-758
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• 2022

Background: Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI. Methods: The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a proteineprotein interaction (PPI) network in STRING database and conducted gene ontology (GO) enrichment analysis. Finally, we assessed the effects of ginseng total saponins (GTS) and different ginsenosides on mitochondrial function by measuring the activity of the mitochondrial respiratory chain complex and performing molecular docking. Results: We screened 2526 MCI-related protein targets by databases and 349 ginsenoside-related protein targets by DARTS. On the basis of these 81 overlapping genes, enrichment analysis showed the mitochondria played an important role in GTS-mediated MCI pharmacological process. Mitochondrial function analysis showed GTS, protopanaxatriol (PPT), and Rd increased the activities of complex I in a dose-dependent manner. Molecular docking also predicted the docking pockets between PPT or Rd and mitochondrial respiratory chain complex I. Conclusion: This study indicated that ginsenosides might alleviate MCI by targeting respiratory chain complex I and regulating mitochondrial function, supporting ginseng's therapeutic application in cognitive deficits.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.405-418
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• 2022

Simotang oral liquid (SMT) is a traditional Chinese medicine (TCM) consisting of four natural plants and is used to alleviate gastrointestinal side effects after chemotherapy and functional dyspepsia (FD). However, the mechanism by which SMT helps cure these gastrointestinal diseases is still unknown. Here, we discovered that SMT could alleviate gastrointestinal side effects after chemotherapy by altering gut microbiota. C57BL/6J mice were treated with cisplatin (DDP) and SMT, and biological samples were collected. Pathological changes in the small intestine were observed, and the intestinal injury score was assessed. The expression levels of the inflammatory factors IL-1β and IL-6 and the adhesive factors Occludin and ZO-1 in mouse blood or small intestine tissue were also detected. Moreover, the gut microbiota was analyzed by high-throughput sequencing of 16S rRNA amplicons. SMT was found to effectively reduce gastrointestinal mucositis after DDP injection, which lowered inflammation and tightened the intestinal epithelial cells. Gut microbiota analysis showed that the abundance of the anti-inflammatory microbiota was downregulated and that the inflammatory microbiota was upregulated in DDP-treated mice. SMT upregulated anti-inflammatory and anticancer microbiota abundance, while the inflammatory microbiota was downregulated. An antibiotic cocktail (ABX) was also used to delete mice gut microbiota to test the importance of gut microbiota, and we found that SMT could not alleviate gastrointestinal mucositis after DDP injection, showing that gut microbiota might be an important mediator of SMT treatment. Our study provides evidence that SMT might moderate gastrointestinal mucositis after chemotherapy by altering gut microbiota.

• Bulletin of the Korean Chemical Society
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• 제34권5호
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• pp.1541-1542
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• 2013

• Journal of Ginseng Research
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• 제47권2호
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• pp.228-236
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• 2023

Background: QiShen YiQi pills (QSYQ) is a Traditional Chinese Medicine (TCM) formula, which has a significant effect on the treatment of patients with myocardial infarction (MI) in clinical practice. However, the molecular mechanism of QSYQ regulation pyroptosis after MI is still not fully known. Hence, this study was designed to reveal the mechanism of the active ingredient in QSYQ. Methods: Integrated approach of network pharmacology and molecular docking, were conducted to screen active components and corresponding common target genes of QSYQ in intervening pyroptosis after MI. Subsequently, STRING and Cytoscape were applied to construct a PPI network, and obtain candidate active compounds. Molecular docking was performed to verify the binding ability of candidate components to pyroptosis proteins and oxygen-glucose deprivation (OGD) induced cardiomyocytes injuries were applied to explore the protective effect and mechanism of the candidate drug. Results: Two drug-likeness compounds were preliminarily selected, and the binding capacity between Ginsenoside Rh2 (Rh2) and key target High Mobility Group Box 1 (HMGB1)was validated in the form of hydrogen bonding. 2 μM Rh2 prevented OGD-induced H9c2 death and reduced IL-18 and IL-1β levels, possibly by decreasing the activation of the NLRP3 inflammasome, inhibiting the expression of p12-caspase1, and attenuating the level of pyroptosis executive protein GSDMD-N. Conclusions: We propose that Rh2 of QSYQ can protect myocardial cells partially by ameliorating pyroptosis, which seems to have a new insight regarding the therapeutic potential for MI.

• 한국한의학연구원논문집
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• 제18권3호
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• pp.133-139
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• 2012

Objective : UMLS is 'Ontology' which establishes the database for medical terminology by gathering various medical vocabularies representing same fundamental concepts. Method : Although Chinese character are represented in the Chinese part of Korean Unicode system in a computer, writing of Chinese characters is vary depending on Chinese input systems and Chinese writers' levels of knowledge. As the result of this, representation of Chinese writing in a computer will be considerably different from an old Chinese document. Therefore, a meaningful relationship between digital Chinese terminology and translated Korean is necessary in order to build Ontology for Chinese medical terms from Oriental medical prescription in a computer system. Result : This research will present 1:1 mapping information among the Chinese characters used in the Oriental medical prescription with analysis of 'same character different sound' and 'same meaning different shape' in Chinese part of Unicode systems. Conclusions : Furthermore, the research will provide top-down menu of relationship between Chinese term and Korean term in medical prescription with assumption of that the Oriental medical prescription has its own unique meaning.

• 국제교류와 융합교육
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• 제3권1호
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• pp.77-86
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• 2023

"일대일로" 전략을 세운 이래 중국은 중의약(中醫藥)을 해외에 보급하였고 더불어 중의약 교육 및 중의약 문화의 현대화라는 큰 성과를 거두었다. 그러나 중의약의 해외 보급 및 발전은 여전히 중국의 문화 강대국으로 발전시키고자 하는 국가의 전략적 요구와 중의약의 역사적 사명 사이의 격차가 여전히 존재하고 있다. 특히, 중의약의 문화 이론, 해외 전파 방식, 전문용어 번역 등에는 여전히 어려움과 이를 해결하고자 하는 도전은 여전히 잔존하고 있다. "일대일로" 전략의 맥락에서 중의약 종사자는 문화적 이상과 자신감을 강화하여야 한다. 또한 중의약 이론의 대중화를 미디어를 충분히 이용하여 강화하여야 한다. 더불어 중의약의 해외 보급 수단을 개선하고, 다문화 언어 교류를 가속화 해야 한다. 이에 중의약 문화의 혁신을 의사 소통 실천으로 실현하고 중의약의 대외 교류와 협력을 강화하며 중의약의 해외 발전을 촉진할 것을 제언하였다.