• Title/Summary/Keyword: Chick myoblast

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Effects of $Ca^2+$ and Protein Kinase C on the Chick Myoblast Differentiation (Ca$^2+$ 및 Protein Kinase C가 배양한 계배근원세포의 분화에 미치는 영향)

  • 정기화;김세재;박정원;박영철;이정주
    • The Korean Journal of Zoology
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    • v.32 no.1
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    • pp.40-47
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    • 1989
  • Alteration of intracellular calcium ion Concentration by adding of either calcium ionophore A23187 or EGTA in culture medium at 24 hr after cell plating resulted in remarkable changes in the progression of differentiation of chick embryo myoblast. When separated myoblast proteins using two-dimensional gel electrophoresis, synthesis patterns of several proteins changed upon the addition of either A23187 or EGTA. Treatment of A23187 and calciumactivated neutral protease at 24 hr after initial plating caused an increase in the rate of fusion compared to control culture. However, EGTA inhibited the myoblast fusion to a marked degree. A23187 treated at 24hr also increased the activity of protein kinase C during the fusionprogressed period. It seems that intracellular calcium ion plays an important role in the myoblast differentiation in vitro together with the protein kinase C and calcium-activated neutral protease.

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Alteration in the Transferrin Receptor during the Chick Myoblast Fusion in Culture (계배 근원세포의 융합에 따른 Transferrin 수용체의 변화)

  • 이창호;유병제;전영주;정진하;하두봉
    • The Korean Journal of Zoology
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    • v.32 no.2
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    • pp.163-175
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    • 1989
  • Transferrin (Tf) has been known to exert profound effect on the myoblast differentiation in uirto. Therefore, the changes in the amount and affinity of the Tf receptor would accompany the myoblast differentiation. To investigate this possibility, we exarnined the afteration pattern in the level of the Tf receptor during the myoblast fusion. The level of Tf receptor was assayed by measuring the bound 125 I-Tf onto the surface of cultured myoblasts, and it was known that the level of Tf receptor reached the maximum at about 12 hr before the initi ation of the myoblast fusion and decreased as the differentiation proceeded, and that the affinity of Tf receptor to Tf was also decreased. In addition, various inhibitiors of the myoblast fusion also influenced the level of the Tf receptor. Accroding to these results, it is postulated that the level of Tf receptor is highly regulated during the myoblast differentiation.

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Identification of a Fusion-associated Protein in the Skeletal Myoblast Using Monoclonal Antibody (단일클론항체를 이용한 배양 계배 근원세포의 융합과 연관된 단백질의 확인)

  • Kim, Chons-Rak;Won
    • The Korean Journal of Zoology
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    • v.35 no.1
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    • pp.29-36
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    • 1992
  • The present study describes the production of monoclonal antibodies against cultured chick myoblast to pursue critical proteins in muscle cell fusion. Among a panel of monoclonal antibodies, three, Mll-3H 13, Mll-3Hl8 and Mll-3H35 were inhibited movblast fusion. A single 101-kDa antigen reactive with monoclonal antibody Mll-3H35 was detected by radioimmu-noprecipitation or by immunoblotting. During the course of myogenesis, the level of the protein remarkably decreased as the cells there differentiated. These results suggest that the protein platys a direct role in the process of myoblast fusion mechanism.

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Effect of Fe3$^+$ on Differentiation of Chick Embryonic Myoblasts Cultured in nitro (배양계배 근원세포의 분화에 미치는 Fe3$^+$의 영향)

  • 유병제;지승완
    • The Korean Journal of Zoology
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    • v.34 no.4
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    • pp.610-617
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    • 1991
  • 계배 근원세포의 분화 및 증식에 미치는 F3+의 영향을 조사하였다. 철이 없는 배양액은 근원 세포의 분화와 증식을 억제하는 것으로 나타났으며 , 따라서 근원세포의 분화에 철과 transferrin (Tf)은 필수적이다. 또한, 철 대신에 Co2+가 부착된 Tf가 첨가된 배양액에서도 근세포의 분화가 정상적으로 일어나는 것으로 나타났다. Lysosomotrophic amine(chloroquine, $\mu$M 수준;ammonium chloride, mM 수준)은 근세포의 분화와 증식을 억제시켰으며 , 근세포 분화의 철에 대한 의존성은 분화됨에 따라 둔화되었다. 세포내로의 T니 수송량은 MEM과 8102배양액에서 비슷하였고, 근세포가 분화됨에 따라 감소하였다. Lysosomotrophic azine은 최소한 3시간이 내에서는 세포내로 수송되는 Tf의 양에 영향을 미치지 못하였다.

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Alterations in the Level of Fibronectin and its Receptors during Chick Myoblast Differentiation (계배 근원세포 분화에 따른 Fibronection의 수준과 그 수용체의 변화)

  • 정창용;강만식
    • The Korean Journal of Zoology
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    • v.31 no.2
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    • pp.95-103
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    • 1988
  • Alterations in the amount of fibronectin during chick myogenesis were investigated. The amount of fibronectin as measured by immunoblotting was found to decrease during the process. As a first step in answering the precise mode of change in the level of ibronedin during myogensis, the interaction of 28,000 dalton(28 kDa) amino terminal fragment of fibronectin as well as 85,000 dalton (85 kDa) fragrxient with myoblasts was examined. The specific binding of 125 l-28 kDa fragment to myoblasts was time-dependent and reached a maximum within 60 min. Unlabelled 28 kDa fragment inhibited the binding of 125 I-28 kDa fragment, whereas 85 kDa fragment containing adhesion promoting activity did not inhibit it. This finding suggests that the 28 kDa fragment interacts with the matrix assembly receptors but not with the cell adhesion receptors. Accordingly, the decrease in the level of fibronectin is likely to correlate with the fall of fibronectin receptors on the myoblasts.

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Changes in the Cellular cGMP Levels and Guanylate Cyclase Activities during Chick Myoblast Fusion (근원세포 융합시 Cellular cGMP 수준과 Guanylate cyclase 활성의 변화)

  • 백미영;강만식
    • The Korean Journal of Zoology
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    • v.36 no.3
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    • pp.433-438
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    • 1993
  • In the previous paper (Choi et al., 1992), we found that a large but transient elevation in intracellular cGMP levels occur concomitant with the myoblast fusion. To establish the physiological significance of the elevation of cGMP levels, the change in guanylate cyclase activity dudng myoblast fusion and the correlation hetween various chemicals that may affect guanylate cyclase adivity and myoblast fusion were examined. Sodium nitroprusside, a nitric oxide-forming compound, induced a precocious fusion and increased guanylate cyclase activity compared to the control. Furthermore, L-NG-monomethyl arginine, specific inhibitor of L-arginine: nitric oxide synthase, inhibited the cell fusion in a dose-dependent manner, without affecting biochemical differentiation. On the basis of our present findings, we propose that the onset of myoblast fusion is somehow correlated with the rise in cellular cGMP levels that is regulated by the activation or inhibItIon of soluble guanylate cyclase, via as yet undefined mechanism but possibly through L-arginine: nitric oxide pathway.

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