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Isolation and Structure Determination of a Proteasome Inhibitory Metabolite from a Culture of Scytonema hofmanni

  • Shim, Sang-Hee;Chlipala, George;Orjala, Jimmy
    • Journal of Microbiology and Biotechnology
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    • 제18권10호
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    • pp.1655-1658
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    • 2008
  • Cyanobacteria, blue-green algae, are a rich source of bioactive secondary metabolites with many potential applications. The ubiquitin-proteasome proteolytic system plays an important role in selective protein degradation and regulates cellular events including apoptosis. Cancer cells are more sensitive to the proapoptotic effects of proteasome inhibition than normal cells. Thus, proteasome inhibitors can be potential anticancer agents. Cyanobacteria have been shown to be a rich source of highly effective inhibitors of proteases. A proteasome inhibitor was screened from an extract of the culture of Scytonema hofmanni on the basis of its inhibitory activity, which led to the isolation of nostodione A with an $IC_{50}$ value of 50${\mu}M$. Its structure was determined by spectroscopic methods such as $^{1}H$-NMR and ESI-MS spectral analyses.

인터넷 기반 전자정보원의 참고서지 기술요소와 형식에 관한 비교분석 (A Comparative Study on the Element and Form of Document Styles for documenting Resources based on Internet)

  • 김홍렬
    • 한국도서관정보학회지
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    • 제33권3호
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    • pp.87-108
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    • 2002
  • 본 연구의 목적은 인터넷을 통해 접근하고 입수가능한 전자정보원을 대상으로 참고문헌 작성기준에 관한 스타일 메뉴얼들의 기술요소와 기재순서, 기술형식을 비교분석하여, 국내 연구자나 학협회, 출판사 등이 참고문헌을 작성하거나 작성지침을 제시할 때 포함할 수 있는 기술요소 및 형식에 관한 자료를 제공하는데 있다. 이를 위하여 최근에 수정보완된 MLA Style, APA Style, CBE Style, Chicago Style, ISO 690-2, SIST-02를 대상으로 그들의 요소와 순서, 표기방법 및 형식을 검토하고, 표기방법 및 형식에 따른 동일자료에 대한 기술사례를 제시하였다.

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PVDF interdigitated transducer for generating and detecting Lamb waves in plates

  • Gu, Hua;Lloyd, George M.;Wang, Ming L.
    • Smart Structures and Systems
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    • 제4권3호
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    • pp.291-304
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    • 2008
  • Piezoelectric materials have been widely used in ultrasonic nondestructive testing (NDT). PZT ceramics can be used to receive and generate surface acoustic waves. It is a common application to attach PZT transducers to the surface of structures for detecting cracks in nondestructive testing. However, not until recently have piezoelectric polymers attracted more and more attention to be the material for interdigitated (IDT) surface and guided-wave transducers. In this paper, an interdigitated gold-on-polyvinylidene fluoride (PVDF) transducer for actuating and sensing Lamb waves has been introduced. A specific etching technology is employed for making the surface electrodes into a certain finger pattern, the spacings of which yield different single mode responses of Lamb waves. Experiments have been performed on steel and carbon fiber composite plates. Results from PVDF IDT sensors have been compared with those from PZT transducers for verification.