• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.29 no.4
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• pp.249-256
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• 2003

The lack of sufficient oral mucosa available for intra-oral reconstruction has been dealt with by the use of skin or oral mucosa grafts harvested from donor sites but grafts requires more than one surgical procedures and could cause donor site morbidity. Many investigators have attempted to increase available soft tissue by tissue engineered skin or oral mucosa replacements for clinical applications. But, reconstructed mucosa by several methods have low physical properties such as rolling and contraction. The aims of this study were to develope an in vitro experimental model that maintains an epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally cultured oral mucosa embedded with Polydioxanone mesh by histological and immunohistochemical analysis. The results were as follows; 1. Oral mucosa reconstructed by three-dimensional organotypic culture revealed similar morphologic characteristics to equvalent normal oral mucosa in the point that they show stratification and differentiation. 2. The expression of cytokeratin 10/13 and involucrin in the cultured tissue showed the same pattern with normal oral mucosa suggesting that organotypic co-culture condition is able to induce cellular differentiation. 3. After insertion of polydioxanone mesh, increased tensile strength were observed. These results suggest that three-dimensional organotypic co-culture of the oral mucosa cell lines with the dermal equvalent consisting type I collagen and fibroblasts reproduce the morphologic and immunohistochemical characteristics similar to those in vivo condition. And increased physical properties by use of polydioxanone mesh will helpful for clinical applications.

• Journal of Environmental Science International
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• v.11 no.12
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• pp.1205-1215
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• 2002

To evaluate the change for water quality after the water gate operation in Shiwha lake, in situ survey were conducted on september in 2000 and January, march, jane in 2001. Chemical characteristics and eutrophication level was estimated from the survey data. The water quality of the Shihwa lake was greatly affected by pollutant load from rainfall, and formation of stratification in summer and winter was increased to effect on nutrient release from sediment. Especially, high concentration of chlorophyll-a was occurred in autumn, due to increased nutrient, high water temperature and low salinity after rainfall runoff. The mean concentration of DIN, DIP were 0.346mg/L, 0.0217mg/L in surface water and 0.826mg/L, 0.0415mg/L in bottom water, respectively, which were over III grade of seawater standard. Also high percentage of ammonia nitrogen to DIN in bottom water for autumn and winter was affected by released nutrient from sediment. Correlation analysis of chlorophyll-a versus TSS was shown that organic matter was affected by autochthonous organic matter stem from the algae, these factor showed reverse correlation about salinity. Closely correlations among to the water quality constituent in continuity survey was appeared. The results of eutrophication index estimation showed the high potentiality of red tide occurrence in Shiwha lake, particularity in summer or fall. Overall water quality was greatly improve to compared with measuring data during 1997~1998 at the beginning water gate operation, which reported by KORDI. Therefore, to improve of water quality in Shiwha lake, we need to establish of management plan about nutrient release from sediment, rainfall runoff, maximum of seawater exchange.

• Ocean and Polar Research
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• v.34 no.2
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• pp.111-123
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• 2012

To understand the phytoplankton community in the eastern part of the Yellow Sea (EYS), in the summer, field survey was conducted at 25 stations in June 2009, and water samples were analyzed using a epifluorescence microscopy, flow cytometry and HPLC method. The EYS could be divided into four areas by a cluster analysis, using phytoplankton group abundances: coastal mixing area, Anma-do area, transition water, and the central Yellow Sea. In the coastal mixing area, water column was well mixed vertically, and phytoplankton was dominated by diatoms, chrysophytes, dinoflagellates and nanoflagellates, showing high abundance ($>10^5\;cells\;l^{-1}$). In Anma-do coastal waters characterized by high dominance of dinoflagellates, high phytoplankton abundance and biomass separated from other coastal mixing area. The southeastern upwelling area was expanded from Jin-do to Heuksan-do, by a tidal mixing and coastal upwelling in the southern area of Manjae-do, and phytoplankton was dominated by benthic diatoms, nanoflagellates and Synechococcus group in this area. Phytoplankton abundance and biomass dominated by pico- and nanophytoplankton were low values in the transition waters and the central Yellow Sea. In the surface of the central Yellow Sea, high dominance of photosynthetic pigments, 19'-hexanoyloxyfucoxanthin and zeaxanthin implies that haptophytes and cyanobacteria could be the dominant group during the summer. These results indicate that the phytoplankton communities in the EYS were significantly affected by the formation of tidal front, thermal stratification, and coastal upwelling showing the differences of physical and chemical characteristics during the summer.

• Journal of Korean Society on Water Environment
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• v.37 no.6
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• pp.425-432
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• 2021

A tidal power plant (TPP) has been in operation since the end of 2011 to improve the water quality of Shihwa Coastal Reservoir (SCR). Tidal mixing rate increased 5.6 times after the TPP operation so that in this study, its effects on water quality was assessed through statistical analysis of long-term water quality monitoring data. It was found that the increased tidal mixing contributed to solving the hypoxia problem in the bottom water by preventing the summer stratification. The analysis also showed that the increased tidal mixing had different effects depending on the relative concentration difference for each water quality substances between the SCR and the outside of SCR. The average concentrations of some substances (chemical oxygen demand, total phosphorus, chlorophyll-a) with higher concentrations than the outside of SCR decreased due to the dilution effect, but the other substances (total nitrogen, dissolved inorganic nitrogen, dissolved inorganic phosphorus) with lower concentrations compared to the outside ones increased on the contrary. Factor analysis also showed a consistent result that the first factor accounting for the water quality was changed from the organic-related substances to the nutrient-related substances after the increased tidal mixing. These results imply that the focus of future water quality management needs shifting from the organic substances to the nutrients, particularly dissolved inorganic nutrients. Considering the effect of inflow seawater on the nutrients, the management area should be extended to cover not only SCR but also a certain area outside of SCR.

• Korean Journal of Soil Science and Fertilizer
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• v.1 no.1
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• pp.129-136
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• 1968

Low-Humic Gley Soils occur very commonly in Korea and constitute important paddy soils of the country. These soils are developed either on alluvial or fluvio-marine materials. The soils in the present invest igation are derived from the latter. The interesting morphological features of these two soils are the presence of $FeCO_3$ concretions in the subsoil and the occurrence of a buried black organic horizon of variable thickness(25-100 cm). The organic horizon overlies the greenish marine material and underlies the continental material. These soils have poor drainage and are wet most of the year. The presence of $FeCO_3$ concretions in poorly drained paddy soils has previously been reported in North America(8), Japan(9) and West Europe(7). The purpose of this investigation is to study the chemical and mineralogical characteristics of these soils; and the genesis of $FeCO_3$ concretions. Profile samples of both these soils were collected from Gimjae area, in co-operation with the Soil Survey Party. These profiles were described as follows:

• Journal of the Korea Organic Resources Recycling Association
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• v.29 no.4
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• pp.33-40
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• 2021

In this study, the physico-chemical and biological characteristics of anaerobic granular sludge at different heights of a full-scale UASB reactor (UASBr) were investigated. Granular sludge was taken from 1 m, 2 m, and 3 m above the ground level in the UASBr. The morphological analysis showed that the upper part had bigger granules and the lower part had rounder granules. The ANOVA test confirmed that the mean size and the roundness of the granules had statistically significant difference along the height at 95% confidence level, and there was a significant negative linear relationship between the size and roundness (r=-0.40, p<0.05). A SMA test using acetic, propionic and butyric acids showed that granules from 2 m height had the highest specific methanogenic activity. The EPS contents were also unequal to each height, with 2 m showing the highest content. These findings could be helpful to understand the different characteristics of stratified anaerobic granules in full-scale UASBr and maintain the reactor performance.

• Korean Journal of Ecology and Environment
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• v.50 no.1
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• pp.1-15
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• 2017

Daecheong Reservoir was made by the construction of a large dam (>15 m in height) on the middle to downstream of the Geum River and the discharge systems have the watergate-spillway (WS), a hydropower penstock (HPP), and two intake towers. The purpose of this study was to investigate the limnological anomalies of turbid water reduction, green algae phenomenon, and oligotrophic state in the lower part of reservoir dam site, and compared with hydro-meteorological factors. Field surveys were conducted in two stations of near dam and the outlet of HPP with one week intervals from January to December 2000. Rainfall was closely related to the fluctuations of inflow, outflow and water level. The rainfall pattern was depended on the storm of monsoon and typhoon, and the increase of discharge and turbidity responded more strongly to the intensity than the frequency. Water temperature and DO fluctuations within the reservoir water layer were influenced by meteorological and hydrological events, and these were mainly caused by water level fluctuation based on temperature stratification, density current and discharge types. The discharges of WS and HPP induced to the flow of water bodies and the outflows of turbid water and nutrients such as nitrogen and phosphorus, respectively. Especially, when hypoxic or low-oxygen condition was present in the bottom water, the discharge through HPP has contributed significantly to the outflow of phosphorus released from the sediment into the downstream of dam. In addition, HPP effluent which be continuously operated throughout the year, was the main factor that could change to a low trophic level in the downreservoir (lacustrine zone). And water-bloom (green-tide) occurring in the lower part of reservoir was the result that the water body of upreservoir being transported and diffused toward the downreseroir, when discharging through the WS. Finally, the hydropower effluent was included the importance and dynamics that could have a temporal and spatial impacts on the physical, chemical and biological factors of the reservoir ecosystem.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.17
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• pp.115-133
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• 1974

This study was done on the metabolism of chemical components during the seed development of ginseng. The changes of the chemical components were inspected in the following periods: from the early stage of flower organ formation to flowering time, from the early stage of fruiting to maturity, during the moisture stratification before sowing. From flower bud forming stage to meiosis stage, the changes in the fresh weight, dry weight, contents of carbohydrates, and contents of nitrogen compounds were slight while the content of TCA soluble phosphorus and especially the content of organic phosphorus increased markedly. From meiosis stage to microspore stage the fresh and dry weights increase greatly. Also, the total nitrogen content increases in this period. Insolub]e nitrogen was 62-70% of the total nitrogen content; the increase of insoluble nitrogen seems to have resulted form the synthesis of protein. The content of soluble sugar (reducing and non-reducing sugar) increases greatly but there was no observable increase in starch content. In this same period, TCA soluble phosphorus reached the maximum level of 85.4% of the total phosphorus. TCA insoluble phosphorus remained at the minimum content level of 14.6%. After the pollen maturation stage and during the flowering period the dry weight increased markedly and insolub]e nitrogen also increased to the level of 67% of the total nitrogen content. Also in this stage, the organic phosphorus content decreased and was found in lesser amounts than inorganic phosphorus. A rapid increase in the starch content was also observed at this stage. In the first three weeks after fruiting the ginseng fruit grows rapidly. Ninety percent of the fresh weight of ripened ginseng seed is obtained in this period. Also, total nitrogen content increased by seven times. As the fruits ripened, insoluble nitrogen increased from 65% of the total nitrogen to 80% while soluble nitrogen decreased from 35% to 20%. By the beginning of the red-ripening period, the total phosphoric acid content increased by eight times and was at its peak. In this same period, TCA soluble phosphorus was 90% of total phosphorus content and organic phosphorus had increased by 29 times. Lipid-phosphorus, nucleic acid-phosphorus and protein-phosphorus also increased during this stage. The rate of increase in carbohydrates was similar to the rate of increase in fresh weight and it was observed at its highest point three weeks after fruiting. Soluble sugar content was also highest at this time; it begins to decrease after the first three weeks. At the red-ripening stage, soluble sugar content increased again slightly, but never reached its previous level. The level of crude starch increased gradually reaching its height, 2.36% of total dry weight, a week before red-ripening, but compared with the content level of other soluble sugars crude starch content was always low. When the seeds ripened completely, more than 80% of the soluble sugar was non-reducing sugar, indicating that sucrose is the main reserve material of carbohydrates in ginseng seeds. Since endosperm of the ripened ginseng seeds contain more than 60% lipids, lipids can be said to be the most abundant reserve material in ginseng seeds; they are more abundant than carbohydrates, protein, or any other component. During the moisture stratification, ginseng seeds absorb quantities of water. Lipids, protein and starch stored in the seeds become soluble by hydrolysis and the contents of sugar, inorganic phosphorus, phospho-lipid, nucleic acid-phosphorus, protein phosphorus, and soluble nitrogen increase. By sowing time, the middle of November, embryo of the seeds grows to 4.2-4.7mm and the water content of the seeds amounts to 50-60% of the total seed weight. Also, by this time, much budding material has been accumulated. On the other hand, dry stored ginseng seeds undergo some changes. The water content of the seeds decreases to 5% and there is an observable change in the carbohydraes but the content of lipid and nitrogen compounds did not change as much as carbohydrates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.17
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• pp.135-142
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• 1974

A study on the metabolism of the chemical companents of endosperm and enbryonic othans of ginseng seeds during their germination were inverstigated and the results of the changes in the contents of nitrogen conponds, carbohydrates, lipids and phosphorus conpounds are summarized as follow; 1. When a seeding grows to 5cm the fresh weight of the embryonic organ incerases 13 times compared with that of its ripened embryo veore germination and its dry weight increases 4.5 times. On the other hand, about 65% of the dry weight of the endosperm is lost. 2. During germinarion the total nitrogen content of a sedding (endosperm+embryonic organ) decreases and when the seeding grows to 5cm there is a loss of 10% of total nitrogen content. At this time, soluble nitrogen content amounts to 40~50% of the total nitrogen, a comparatively high content. 3. When theseeding grows to 5cm, the total phosphorus content decreases by 15%. During the germination period 70~75% of the total phosphorus is distributed in the embryonic orang and 25% of it is in endosperm.In the embryonic organ 35~50% of the acid soluble phosphorus is inorganic phosphorus and in the endisperm, 20~25% of the acid soluble phosphorus is inorganic phosphorus,75~80% of the organic phosphorus is contained in the endosperm. 4.One the seedling grew to 2~3cm, carbohydrates such as soluble sugars,reducong sugars,nonreducong sugars, and crude starch interconverted remarkably. 5.After stratification (just before germination) the lipid content of the endosperm is about 54% of the total weight and lipid content of the embryo is about 61%. During germination 6.81mg of the fat contained in the endosperm per seed decreases to 4.13mg while the change of fat content in the embryonic organ is not so great.

• Korean Journal of Ecology and Environment
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• v.33 no.3 s.91
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• pp.213-221
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• 2000

Seasonal oxygen content and deficit rates were evaluated from 17 sites of Taechung Reservoir during 1993${\sim}$1994. In 1993, river inflows peaked during the monsoon in July${\sim}$August and disrupted thermal stratification and anoxic layers in the headwaters, thereby confining the anoxia to the mid-lake and downlake reach. The volume of anoxic water with < 4 mg/l DO comprised only < 10% of the total lake volume in this period. In contrast, during monsoon 1994, 85% of total lake volume was subject to hypoxic conditions with oxygen concentrations < 30% saturation, resulting in massive fishkills (Hypomesus olidus). Relative areal oxygen deficit (RAOD) was -0.024mg O$_{2}$cm$^{-2}$d$^{-1}$ during monsoon 1993, whereas it rapidly decreased at the rate of 0.080mg O$_{2}$cm$^{-2}$d$^{-1}$ during monsoon 1994. Anoxic factor (AF) showed a same interannual pattern as the RAOD and was greater >50 d in 1994 (76.5 d) than 1993 (21.3 d). Thus, the reservoir showed a river-characteristics (6${\sim}$11 mg/l DO) in 1993 while lacustrine conditions (<4mg/l DO) dominated in 1994. Regression analysis showed that the variation of summer DO was mostly determined (R$^{2}$=0.99, p<0.0001) by inflow. These findings suggest that the primary factor regulating the oxygen content in this system during summer is an intensity of the monsoon rain.