• Title/Summary/Keyword: Chemical laboratory

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Production, Purification, and Characterization of Taxol and 10-DABIII from a new Endophytic Fungus Gliocladium sp. Isolated from the Indian Yew Tree, Taxus baccata

  • Sreekanth, D.;Syed, A.;Sarkar, S.;Sarkar, D.;Santhakumari, B.;Ahmad, A.;Khan, M.I.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.11
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    • pp.1342-1347
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    • 2009

  • We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded $10\;{\mu}g$ of taxol and $65\;{\mu}g$ of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

  • https://doi.org/10.4014/jmb.0904.04041 인용 PDF KSCI

Improved performance of polyamide nanofiltration membranes by incorporating reduced glutathione during interfacial polymerization

  • Jiao, Zhiwei;Zhou, Linjie;Wu, Mengyuan;Gao, Kang;Su, Yanlei;Jiang, Zhongyi
    • Korean Journal of Chemical Engineering
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    • v.35 no.12
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    • pp.2487-2495
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    • 2018

  • Inspired by the specific amino acid sequence Asn-Pro-Ala (NPA) of water channel aquaporins (AQPs), we fabricated polyamide (PA) nanofiltration (NF) membranes by introducing reduced glutathione (GSH) in interfacial polymerization (IP) method. Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectrometry (XPS), scanning electron microscope (SEM), atomic force microscopy (AFM), zeta potential and static water contact angle measurement were employed to characterize the chemical composition, morphology, electronegativity and hydrophilicity of the NF membranes. The water flux of GSH/PIP-TMC NF membrane reached $32.00L\;m^{-2}h^{-1}$ at 0.2 MPa, which was approximately twice than that of pristine PIP-TMC NF membrane when the ratio of GHS to piperazidine (PIP) was 40% during IP process. More water channels were built as GSH was embedded into PA layer. The fabricated NF membranes also took on potent rejection for dyes and $Na_2SO_4$. This study presents a simple and facile method to simulate water channels-based biological materials which may find potential application in water treatment.

  • https://doi.org/10.1007/s11814-018-0153-2 인용 KSCI

12-Hydroxyamoorastatone, a New Limonoid from Melia azedarach var. Japonica

  • Jong-Woong Ahn;Geon-Seek Yoo;Zaesung No;Seung-Ho Lee
    • Bulletin of the Korean Chemical Society
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    • v.14 no.5
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    • pp.554-556
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    • 1993

  • A chemical investigation of the stem bark of Melia azedarach var. Japonica (Meliaceae) has led to a new limonoid, 12-hydroxyamoorastatone (1), whose structure has been elucidated by spectroscopic measurements including 2D-NMR. The 2D-NOESY experiment on its di-p-bromobenzoate derivative (1a) has established the relative configuration of 1.

  • https://doi.org/10.5012/bkcs.1993.14.5.554 인용 PDF