• Toxicological Research
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• v.13 no.1_2
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• pp.111-116
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• 1997

In the present study, we investigated the neutralization activity of various antimicrobials against lipopolysaccharide (LPS) as well as interaction between two antimicrobials (enrofioxacin and coilstin) using checkerboard method. The neutralization activity of antimicrobials used in the test was assayed by means of LAL chromogenic test after reaction of LPS with colistin, enorfioxacin, ampicillin, polymyxin B, oxytetracycline, streptomycin, and erythromycin. As the results, the neutralization activity of coltstin and polymixin B had a more stronger than that of tested other antimicrobials. In bacterial culture broth, the best neutralization activity of the antibiotics was also shown to coltstin and polymixin B. Meanwhile, It was shown to have synergism between enorfloxacin and coltstin on the basts of FIC (fractional inhibition concentration). The FIC of enorfioxacin-colistin combinations was 0.50-1.03 to Staphylococcus aureus R-209, 1.03-1.06 to Salmonella typhimurium, 0.75-1.25 to Bordetella Bronchtseptica and 1.02-1.25 to E. coli K88ab. On the basts of the above results, the present study may be of clinical usefulness in the choice of an antibiotic therapy for severe sepsts in animals.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.12C
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• pp.1010-1019
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• 2010

This article presents a PDE-based interpolation algorithm to effectively reproduce high resolution imagery. Conventional PDE-based interpolation methods can produce sharp edges without checkerboard effects; however, they are not interpolators but approximators and tend to weaken fine structures. In order to overcome the drawback, a texture enhancement method is suggested as a post-process of PDE-based interpolation methods. The new method rectifies the image by simply incorporating the bilinear interpolation of the weakened texture components and therefore makes the resulting algorithm an interpolator. It has been numerically verified that the new algorithm, called the PDE-based image interpolator (PII), restores sharp edges and enhances texture components satisfactorily. PII outperforms the PDE-based skeleton-texture decomposition (STD) approach. Various numerical examples are shown to verify the claim.

• Journal of the Optical Society of Korea
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• v.19 no.4
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• pp.397-402
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• 2015

In this paper, we propose a method used to measure the polarization state of a field with multiple polarization singularities (PSs), such as an array of PSs and PSs in vector speckle. A checkerboard is used to construct characteristic points, which makes it possible to calibrate the mismatches between pictures obtained during the rotation of a quarter-wave plate and a polarizer. Using this method a field with an array of PSs is measured. The experimental result is compared with the numerical simulation. We also carry out some data analysis. The comparison and analysis emphasize the necessity and feasibility of this method to measure the PSs.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.221-227
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• 1999

The fungistatic and fungicidal activities of amphotericin B, fluconazole, miconazole, econazole, and 5-fluorocytosine against Saccharomyces cerevisiae were estimated in the presence of 1/2 minimum inhibitory concentration (MIC) and 1/2 minimum fungicidal concentration (MFC) of polygodial, respectively. Among them, the antifungal activities of miconazoles by polygodial was still shown against several yeast-like fungi including Candida albicans, Candida utilis, Cryptococcus neoformans, except for Candida krusei. The combination of polygodial with imidazole drugs against Saccharomyces cerevisiae was further examined using the macrobroth dilution checkerboard method. The fractional inhibitory concentration (FIC) and the fractional fungicidal concentration (FFC) index between polygodial and miconazole were 0.16 and 0.19, while the combination of polygodial with econazole exhibited the FIC index of 0.19 and the FFC of 0.25, respectively. These results suggest that polygodial and the imidazoles on the fungistatic and fungicidal action are highly synergistic.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.228-232
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• 1999

The combination effect of anethole with amphotericin B, fluconazole, miconazole, or 5-fluorocytosine was investigated against Saccharomyces cerevisiae. When combined with $\frac{1}{2}$ minimum inhibitory concentration (MIC) or $\frac{1}{2}$ minimum fungicidal concentration (MFC) of anethole, the antifungal activities of fluconazole and 5-fluorocytosine were not changed, but the fungistatic and the fungicidal activities of miconazole were increased 64-fold, respectively. In the case of amphotericin B, the fungistatic activity was increased 2-fold, while the fungicidal activity was decreased 2-fold. The combination effect of anethole with miconazole or amphotericin B was also investigated at the various concentrations using the macrobroth dilution checkerboard method. The fractional inhibitory concentration (FIC) and the fractional fungicidal concentration (FFC) index between B exhibited the FIC index of 8.25 and the FFC of 32.06, respectively. Thus, it is analyzed that the combination of anethole with miconazole or amphotericin B on the antifungal action shows synergism and antagonism, respectively.

• Journal of Mechanical Science and Technology
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• v.17 no.4
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• pp.545-561
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• 2003

We use the first-passage-time formulation by Torquato, Kim and Cule ［J. Appl. Phys., Vol. 85, pp. 1560∼1571 (1999) ］, which makes use of the first-passage region in association with the diffusion tracer's Brownian movement, and develop a new efficient Brownian motion simulation method to compute the effective conductivity of digitized composite media. By using the new method, one can remarkably enhance the speed of the Brownian walkers sampling the medium and thus reduce the computation time. In the new method, we specifically choose the first-passage regions such that they coincide with two, four, or eight digitizing units according to the dimensionality of the composite medium and the local configurations around the Brownian walkers. We first obtain explicit solutions for the relevant first-passage-time equations in two-and three-dimensions. We then apply the new method to solve the illustrative benchmark problem of estimating the effective conductivities of the checkerboard-shaped composite media. for both periodic and random configurations. Simulation results show that the new method can reduce the computation time about by an order of magnitude.

• Journal of Veterinary Clinics
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• v.17 no.1
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• pp.21-27
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• 2000

고양이 말초혈액 다형핵백혈구 (PMN)의 유주활성에 있어서 계난백유래물질 (EWD)의 면역증강 효과를 검토하였다. PMN의 유주활성은 EWD 그 자체 및 EWD로 배양한 PMN의 배양상층액에서는 활성이 존재하지 않았다. 그러나 EWD로 배양한 MNC 배양상층액에서는 PMN에 대한 유주활성이 증가되었다. 본 활성은 checkerboard assay를 통해 진성 유주활성으로 밝혀졌다. 이러한 고양이 PMN 유주활성은 human recombinant interleukin-8(hr IL-8)에 의해서도 증가되었다. IL-8의 또 다른 특성인 세포 형태변형 실험에서도 EWD로 배양한 MNC의 배양상층액과 hr IL-8 모두에서 높은 수준의 세포 형태변형효과를 나타내었다. 이와 같이 EWD로 배양한 MNC의 배양상층액과 hr IL-8 모두에서 증가된 PMN의 유주활성 및 세포변형효과는 anti-human IL-8mAb에 의해 농도의존적으로 억제되었다. 이상의 결과로부터 EWD는 고양이 PMN의 유주활성에 있어서 면역증강 효과가 있으며, 이것은 EWD에의해 활성화된 MNC에서 분비되는 IL-8양 유주성 인자에 의해 PMN의 유주활성 증가 및 세포 형태 변형이 일어나는 것으로 사료되었다.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.572-577
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• 2007

The paper presents design optimization of an automobile body for dynamic stiffness improvement. The thicknesses of plates making-up the monocoque body of an automobile were employed as design variables for optimization and the objective was to increase the first torsional and bending natural frequencies. By allotting one design variable to each plate of the body, compared to previous works based on element-wise design variables, design space of optimization was reduced to a large extent and numerical instabilities such as checkerboard pattern was efficiently evaded. The method resulted to a considerable amount of increase in the automobile body's torsional and bending natural frequencies. Considering manufacturability of the optimized result, the converged values of plate thicknesses were approximated to commercially-available values by appropriately reflecting their design sensitivities.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.467-472
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• 2007

A modern MEMS resonator is a micro-scale structure operated over a high frequency range. In order to predict its resonant behavior in a design process, High-frequency response analysis (Hi-FRA) is demanded. Algebraic substructuring (AS) is known as a fast numerical technique to construct an eigenspace for FR and frequency sweep (FS) algorithm efficiently solves the frequency response system projected on the eigenspace. However, the existing FS algorithm using AS is developed for low-FRA, say over the range 1Hz-2KHz. In this work, we extend the FS algorithm using AS for FRA over an arbitrary frequency range. Therefore, it can be efficiently applied to systems operated at a high frequency, say over the range 230MHz-250MHz. The success of the proposed method is demonstrated by Hi-FRA of a checkerboard resonator.

• Natural Product Sciences
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• v.17 no.4
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• pp.296-302
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• 2011

We determined the inhibitory activity of the essential oil fraction obtained by steam distillation from the fresh and dried leaves of Perilla frutescens var. acuta against some pathogenic Salmonella and Vibrio spp. The activities of compounds isolated from the essential oils, apiol and myristicin, were also tested and the results were compared with those of the essential oil fraction. The Perilla essential oil fraction and its main components showed significant inhibition against antibiotic-susceptive and antibiotic-resistant strains of the tested Salmonella and Vibrio strains. Synergistic or additive effects were identified by combing the oils with ampicillin by checkerboard-titer tests. We conclude that essential oils from P. frutescens can be useful in the treatment of Salmonella and Vibrio infections and as safe additives to food materials for the prevention of contamination of food by these bacteria. This is especially important because of the rapid increase in antibiotic-resistant strains, which could cause severe symptoms in humans.