• YAKHAK HOEJI
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• v.51 no.5
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• pp.355-360
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• 2007

The essential oil fraction from the aerial parts of A. annua was analyzed by GC-MS. As the results, caryophyllene oxide (11.7%), caryophyllene (7.54%), camphor (7.32%), 1,8-cineol (4.98%), and borneol (3.99%) were confirmed as the main components of the oil fraction. The effects of this oil and its main components on antibiotic-susceptible and -resistant strains of Salmonella enteritidis and S. typhimurium were assessed. A. annua oil fraction significantly inhibited all strains of the two Salmonella species examined, with minimum inhibiting concentrations (MICs) ranging from 2.0 mg/ml to 8.0 mg/ml. Among the main components of the oil, borneol and camphor showed relatively strong inhibiting activity with MICs between 1.0 mg/ml and 4.0 mg/ml. The MICs of caryophyllene and caryophyllene oxide were higher than 16 mg/ml. The combination effects of the oils with kanamycin were evaluated using a checkerboard microtiter assay. Against S. typhimurium KCCM11862 and CCARM8009 strains, the oil fraction of A. annua, camphor, and 1,8-cineol exhibited significant synergistic with kanamycin with fractional inhibitory concentration (FIC) indices in the range of 0.085 to 0.375. In conclusion, a combination of kanamycin and A. annua oil or its main component, camphor, and cineol, may be useful for reducing the minimum effective dose of antibiotic required for the treatment of resistant S. typhimurium infections.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2003.04a
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• pp.184-187
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• 2003

Complete prediction of second order permeability tensor for three dimensional circular braided preform is critical to understand the resin transfer molding process of composites. The permeability can be predicted by considering resin flow through the multi-axial fiber structure. In this study, permeability tensor for a 3-D circular braided preform is calculated by solving a boundary problem of a periodic unit cell. Flow field through the unit cell is obtained by using a 3-D finite volume method (FVM) and Darcy's law is utilized to obtain permeability tensor. Flow analysis for two cases that a fiber tow is regarded as impermeable solid and permeable porous medium is carried out respectively. It is found that the flow within the intra-tow region of the braided preform is negligible if inter-tow porosity is relatively high but the flow through the tow must be considered when the porosity is low. To avoid checkerboard pressure field and improve the efficiency of numerical computation, a new interpolation function for velocity variation is proposed on the basis of analytic solutions. Permeability of the braided preform is measured through a radial flow experiment and compared with the permeability predicted numerically.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.900-907
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• 2000

The three-dimensional turbulent flow in curved pipes susceptible to flow-accelerated corrosion has been analyzed numerically to predict the pressure and shear stress distributions on the inner surface of the pipes. The analysis employs the body-fitted non-orthogonal curvilinear coordinate system and a standard $ {\kappa}-{\varepsilon}$ turbulence model with wall function method. The finite volume method is used to discretize the governing equations. The convection term is approximated by a high-resolution and bounded discretization scheme. The cell-centered, non-staggered grid arrangement is adopted and the resulting checkerboard pressure oscillation is prevented by the application of a modified version of momentum interpolation scheme. The SIMPLE algorithm is employed for the pressure and velocity coupling. The numerical calculations have been performed for two curved pipes with different bend angles and curvature radii, and discussions have been made on the distributions of the primary and secondary flow velocities, pressure and shear stress on the inner surface of the pipe to examine applicability of the present analysis method. As the result it is seen that the method is effective to predict the susceptible systems or their local areas where the fluid velocity or local turbulence is so high that the structural integrity can be threatened by wall thinning degradation due to flow-accelerated corrosion.

• Journal of Korean Medicine Rehabilitation
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• v.23 no.3
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• pp.15-26
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• 2013

Objectives The aim of this study was to investigate antimicrobial activity of Huanggeumjakyak- tang water extract against MRSA. Methods The antibacterial activities of Huanggeumjakyak-tang were evaluated against 3 strains of Methicillin-resistant Staphylococcus aureus (MRSA) and 1 standard Methicillinsusceptible S. aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay was performed under dark. Results The MIC (minimum inhibitory concentration) of Huanggeumjakyak-tang water extract against S. aureus strains ranged from 1,000 to $2,000{\mu}g/ml$. So we confirmed that it has a strong antibacterial effect. Also the combinations of Huanggeumjakyak-tang water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. Conclusions The results obtained in this study suggest that Huanggeumjakyak-tang water extract showed antibacterial effect against MRSA, and it also showed reducing effect on the side-effect problems that are the major weak points of traditional antibiotics.

• Journal of KIBIM
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• v.7 no.1
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• pp.45-53
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• 2017

Continuous research efforts have been made on acquiring location data on construction sites. As a result, GPS and RFID are increasingly employed on the site to track the location of equipment and materials. However, these systems are based on radio frequency technologies which require attaching tags on every target entity. Implementing the systems incurs time and costs for attaching/detaching/managing the tags or sensors. For this reason, efforts are currently being made to track construction entities using only cameras. Vision-based 3D tracking has been presented in a previous research work in which the location of construction manpower, vehicle, and materials were successfully tracked. However, the proposed system is still in its infancy and yet to be implemented on practical applications for two reasons. First, it does not involve entity matching across two views, and thus cannot be used for tracking multiple entities, simultaneously. Second, the use of a checker board in the camera calibration process entails a focus-related problem when the baseline is long and the target entities are located far from the cameras. This paper proposes a vision-based method to track multiple workers simultaneously. An entity matching procedure is added to acquire the matching pairs of the same entities across two views which is necessary for tracking multiple entities. Also, the proposed method simplified the calibration process by avoiding the use of a checkerboard, making it more adequate to the realistic deployment on construction sites.

• Tribology and Lubricants
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• v.33 no.5
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• pp.202-206
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• 2017

In this study, the hydrophobicity properties for riblet surfaces that replicate shark skin are simulated. Riblet surfaces with surface roughness on riblets are generated numerically based on the measured data of real shark skin. We assumed that a rib on a scale is hemi-elliptical surface. The surface used in the simulation for the calculation of contact angle is composed of 9 scales like checkerboard type with a roughness. The contact angle of a water droplet can be calculated using the Wenzel equation and Cassie-Baxter equation for the generated riblet surfaces. The variation of contact angles with a fractional depth of penetration for the generated shark skin surfaces without and with coatings is demonstrated in the condition of solid-air-water. The results show that the contact angle for the surface without coating decreases with an increase of the fractional depth of penetration more drastically than that for the surface with coating. We compared the experimental and simulated results. It is shown that the measured contact angles of the shark skin template and the shark skin replica are within the simulated results. Therefore the contact angle of water droplet for rough surfaces can be estimated by the developed numerical method in this study.

• The Journal of the Korean Society for Microbiology
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• v.21 no.1
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• pp.63-71
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• 1986

The antifungal activities of amphotericin B, 5-fluorocytosine, and ketoconazole in combination of amphotericin B/ketoconazole and 5-fluorocytosine/ketoconazole were determined against 42 strains of Candida spp. isolated from oral cavity. Among 42 strains of Candida species, 36 strains of Candida albicans, 2 strains of Candida parapsilosis and Candida tropicalis 1 strain of Candida krusei and Candida stellatoidea were identified. The minimum inhibitory concentrations(MICs) of amphotericin B, 5-fluorocytosine and ketoconazole for these strains were ranged from 0.05-1.56 mcg/ml, 12.5->100.0 mcg/ml and 0.2-50.0 mcg/ml. In all of the experimental strains, amphotericin B had the greatest antifungal activity on a dilution basis. When a microtiter checkerboard technique was used 5-fluorocytosine acted synergistically with ketoconazole against all strains, whereas amphotericin B has a reduced effect. The killing curve experiments with on strain of Candida albicans WMC-85024 demonstrated that the combination of amphotericin B/ketoconazole and 5-fluorocytosine/ketoconazole produced a decrease in number of colony forming unit of >3 logs in 72 hours.

• Food Science and Biotechnology
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• v.18 no.5
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• pp.1263-1272
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• 2009

Danshen (Salvia miltiorrhiza Bunge) is a traditional Korean medicine that is commonly used for the treatment of inflammatory diseases such as edema, arthritis, hepatitis, and endangitis. The several extracts of danshen were tested for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) isolated in clinic by broth microdilution method, the checkerboard, and time-kill methods to evaluate the synergistic effects of the combination of the extracts with antibiotics. The chloroform ($CHCl_3$) and n-hexane (HEX) extracts [minimum inhibitory concentration (MIC), 0.0078-0.3125${\mu}g/mL$; minimum bactericidal concentration (MBC), 0.019-0.625${\mu}g/mL$] were found to have strong antibacterial activity against MRSA. Additionally, when the $CHCl_3$ and HEX extracts were co-administered with ampicillin or oxacillin, a synergistic effect against MRSA was observed. Furthermore, a time-kill study evaluating the effects of the extracts against MRSA indicated that treatment with the $CHCl_3$ extract in combination with ampicillin or oxacillin produced rapid bactericidal activity. These results suggest that danshen extracts may have potently antimicrobial activity and thus, it can be a suitable phytotherapeutic agent for treating MRSA infections.

• Structural Engineering and Mechanics
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• v.45 no.6
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• pp.759-777
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• 2013

In this paper, a topology optimization method based on the element independent nodal density (EIND) is developed for continuum solids with multiple load cases and multiple constraints. The optimization problem is formulated ad minimizing the volume subject to displacement constraints. Nodal densities of the finite element mesh are used a the design variable. The nodal densities are interpolated into any point in the design domain by the Shepard interpolation scheme and the Heaviside function. Without using additional constraints (such ad the filtering technique), mesh-independent, checkerboard-free, distinct optimal topology can be obtained. Adopting the rational approximation for material properties (RAMP), the topology optimization procedure is implemented using a solid isotropic material with penalization (SIMP) method and a dual programming optimization algorithm. The computational efficiency is greatly improved by multithread parallel computing with OpenMP to run parallel programs for the shared-memory model of parallel computation. Finally, several examples are presented to demonstrate the effectiveness of the developed techniques.

• Structural Engineering and Mechanics
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• v.51 no.5
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• pp.793-808
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• 2014

The goal of this study is to evaluate and design steel plates with optimal material distributions achieved through a specific material topology optimization by using a CCARAT (Computer Aided Research Analysis Tool) as an optimizer, topologically optimally updating node densities as design variables. In typical material topology optimization, optimal topology and layouts are described by distributing element densities (from almost 0 to 1), which are arithmetic means of node densities. The average element densities are employed as material properties of each element in finite element analysis. CCARAT may deal with material topology optimization to address the mean compliance problem of structural mechanical problems. This consists of three computational steps: finite element analysis, sensitivity analysis, and optimality criteria optimizer updating node densities. The present node density based design via CCARAT using node densities as design variables removes jagged optimal layouts and checkerboard patterns, which are disadvantages of classical material topology optimization using element densities as design variables. Numerical applications that topologically optimize reinforcement material distribution of steel plates of a cantilever type are studied to verify the numerical superiority of the present node density based design via CCARAT.