• Journal of Ecology and Environment
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• v.29 no.3
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• pp.185-189
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• 2006

Optimum tapping size and number for sap collection were investigated to minimize damage on tree growth and secure resources for sap by sap collection of Acer mono. The amounts of sap collected by tapping size of 12 mm, 10 mm and 8 mm was 189 L, 140 L and 193 L, respectively. Fusion rates by tapping size were 100% for 8 mm, 89% for 10 mm and 85% for 12 mm, respectively. Amount of sap by size and number of tapping were much at three, two and three tappings to small, middle and large diameter tree in case of 8 mm tapping, and it was large in quantity at two tappings of 12 mm tapping to large diameter tree, respectively. Trees tapped from one to three tappings of 8 mm size for small diameter tree with treatment of DB ointment (mixture of thiophane ointment and 2% bamboo charcoal powder) were completely filled up within 6 months. Diameter growth by number of tapping of 8 mm size was 0.60 mm for one tapping, 1.12 mm for two tappings and 0.47 mm for three tappings to small, middle and large diameter tree, respectively. In case of 12 mm tapping size, diameter growth was fast in the order of large (0.55 mm), middle (0.30 mm) and small (0.23 mm) diameter tree, respectively.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.143-151
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• 2010

In Vivo and In vitro antispasmodic effects of Jun-Si-Baek-Chul-San, a Traditional Korean Polyherbal Medicineconsisted of 7 types of herbs were observed in the present study. To clarify the effects of Jun-Si-Baek-Chul-San, on accelerating small intestinal movement induced by the stimulation of cholinergic neurotransmission, we evaluated the effects of Jun-Si-Baek-Chul-San on In vivo carbachol (an acetylcholinergic agent)-accelerated mice small intestinal transit and on In vitro contractions induced by low-frequency electrostimulation, KCl, histamine or acetylcholine using isolated guinea pig ileum. To induce the acceleration of mice small intestinal transit, Carbachol 1 mg/kg was once subcutaneously dosed 15min before last administration of the test drugs. In the present study, Jun-Si-Baek-Chul-San 500, 250 and 125 mg/kg or domperidone 20 mg/kg were orally pretreated on the carbachol-accelerated mice small intestinal transit once a day for 7 days and the small intestinal transit rateof activated charcoal powder were monitored. In vitro assays, Jun-Si-Baek-Chul-San1, 0.1, 0.01 and 0.001 mg/ml or domperidone $2{\times}10^{-5}M$ were treated 10min before ileal contraction was induced by filed stimulation, acetylcholine, KCl and histamine, and the % changes of contractions were observed compared to the treatment of inducer alone. In spontaneous contraction, the % changes of contractions were observed compared to treatment of vehicle alone at 10min after Jun-Si-Baek-Chul-San or domperidone treatment. The efficacy of Jun-Si-Baek-Chul-San was compared to those of domperidone. High concentration, 1 mg/ml of Jun-Si-Baek-Chul-San was found to decrease the spontaneous contraction of the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San decrease contractions induced by electrostimulation, acetylcholine, histamine and KCl in the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San effectively inhibited the accelerated small intestinal movement induced by carbachol stimulation of cholinergic neurotransmission in In vivo. Based on the results, although the exact molecular or action mechanism and which herbs or compound in Jun-Si-Baek-Chul-San are responsible for actions, it was concluded that Jun-Si-Baek-Chul-San normalization in the accelerated intestinal motility might be interfere with a variety of muscarinic, adrenergic and histaminic receptor activities or with the mobilization of calcium ions required for smooth muscle contraction non-specificly. Therefore, it is expected that Jun-Si-Baek-Chul-San will be promising as a prescription of clinical treatment of digestive tract disorders such as accelerated the motility of intestine, diarrhea or intestinal painful contractions.