• IMMUNE NETWORK
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• v.7 no.3
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• pp.109-116
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• 2007

Background: Human papillomavirus (HPV) infection is responsible for cervical cancer, a common cancer in women. Since HPV infection and cancer development are controlled by the host immune system, immunotherapy against HPV can be helpful in preventing or treating HPV-associated cervical cancer. Two oncoproteins of HPV16, E6 and E7, are promising targets for immunotherapy against cervical cancer, because they are constitutively expressed in cervical cancer. Methods: Since cellular vaccines using B cells as well as dendritic cells offer an efficient approach to cancer immunotherapy, we opted to use B cells. We evaluated the immunogenicity and anti-tumor effects of a B cell vaccine transduced with HPV16 E6/E7-expressing adenovirus. Results: Vaccination with HPV16 E6/E7-transduced B cells induced E6/E7-specific $CD8^+$ T cell-dependent immune responses and generated anti-tumor effects against E6/E7-expressing TC-1 tumor. The anti-tumor effect induced by this B cell vaccine was similar to that elicited by DC vaccine, showing that B cells can be used as an alternative to dendritic cells for cellular vaccines. Conclusion: Thisstudy has shown the feasibility of using B cells as immunogenic APCs and the potential for developing prophylactic and therapeutic vaccines against HPV-associated cervical cancer using a B cell vaccine transduced with adenovirus expressing HPV16 E6/E7.

• Radiation Oncology Journal
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• v.9 no.2
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• pp.337-342
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• 1991

The comparison of the KAPM Dosimetric Protocol (1990) with the TG-21 and $C_{\lambda}/C_E$(ICRU-21 and SCRAD protocol) method is studied. The therapetutic range of radiation (photon 4MV-l5MV and electron 6 MeV-20MeV) and three kinds of the chambers were used in the water phantom. The results from 7G-21 and KAPM protocol did not show much differences (less than 1$\%$) throughout the whole energy range; $N_D$ from KAPM protocol and Ngas from TG-21 showed 0.2$\%$ deviation mainly from W/e difference between two protocols. But the results from KAPM protocol (1990) and those from $C_{\lambda}/C_E$ Method showed $-1.9{\pm}0.6\%$ (KAPM protocol is higher) deviation for photom beam and $+3.3{\pm}1\%$ (KAPM protocol is lower) deviation for electron beams.

• The Journal of Information Systems
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• v.18 no.3
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• pp.1-25
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• 2009

As many universities introduce e-Learning classes as formal courses, numerous research topics relating to c-Learning such as, defining e-Learning, identifying factors affecting successful e-Learning deployment and examining relationships between the factors in e-Learning classes need to be focused on. However, most researches thai have been undertaken only consider the positive side or right functional dimension. This can result in e-Learning dissemination at universities being overlooked. In accordance with this indispensability, the negative factors, which are potentially inherent in e-Learning learner's perception and affect personnel e-Learning acceptance in university classes need 10 be acknowledged. The purpose of this study was to identify the negative factors affecting personnel e-Learning acceptance and to analyze the interrelation among the factors in this research model. The two independent variables avoidable convenience and reliant convenience, based on pilot test results, and self-efficacy and perceived playfulness, based on the relevant literature, are used to examine the research model. The research problem was tested with data collected from 446 respondents in 12 universities. This study developed and empirically analyzed a model representing the relationship by using the Structural Equation Model. The major findings of this study are, firstly, that the higher reliant convenience is negatively affecting the degree of system use and learner satisfaction, whereas avoidable convenience is only affecting the learner satisfaction. Secondly, the higher self-efficacy and stronger perceived playfulness affects the degree of system use as well as learner satisfaction. Finally, the degree of system use affects the learner satisfaction.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.162-166
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• 2002

(Z)-ocimene+limonene, $(E)-{\beta}-ocimene$ and citronellal showed seasonal variation in the leaves of Z. schinifolium. Especially estragole was detected at fruiting stage regardless of collection sites. Common variation components in the leaves of Z. piperitum at all collection sites were (Z)-3-hexenol, ${\alpha}-pinene$, limonene and citronellal. The compositions with monthly variation in Z. schinifolium at arboretum were ${\alpha}-pinene$, myrcene, (Z)-3-hexenyl acetate, ${\alpha}-phellandrene$, (Z)-ocimene+limonene, ${\beta}-phellandrene$, linalool, geranyl acetate while in Z. piperitum were hexanal, (Z)-3-hexenol, (E)-2-hexenal, hexanol, ${\alpha}-pinene$, (Z)-ocimene, limonene, citronellal, geranyl acetate, ${\beta}-caryophyllene$. Estragole was not detected in Z. schinifolium leaves at arboretum due to too young tree to bearing fruit on it.

• Korean Journal of Plant Taxonomy
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• v.41 no.3
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• pp.259-266
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• 2011

Elsholtzia hallasanensis, E. springia, and E. splendens var. fasciflora (Lamiaceae) were reported recently as new species or new varieties of E. splendens according to their morphological characteristics. To reappraise the taxonomic status of these additional taxa and to determine the relationships between all Korean Elsholtzia taxa except E. saxatilis, which is distributed in North Korea, molecular studies based on the nrDNA (ITS) and cpDNA (rpl16, and trnH-psbA) sequences of seven taxa of Elsholtzia and one outgroup were carried out. The molecular data support that E. angustifolia and E. minima are distinct species from E. splendens and E. ciliata, respectively, because they have several private marker genes and show monophyly. The molecular data also support that E. splendens has a very close taxonomic relationship with both E. hallasanensis and E. springia. We found that E. splendens var. fasciflora, with multiple inflorescence, was based on several private marker genes and on the monophyly of its trees, suggesting that it can be considered as a variety. Elsholtzia springia, with the same sequences and the same morphological characteristics with E. hallasanensis after transplanting, should be treated as a synonym of E. hallasanensis. Moreover, we consider the taxonomic status of E. hallasanensis as E. splendens var. hallasanensis (Y. Lee) N.S. Lee & C.S. Lee, stat. nov.

• Bulletin of the Korean Chemical Society
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• v.23 no.1
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• pp.48-52
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• 2002

Using several molecular modeling programs we have performed computer simulations to investigate the complexation behaviors of an ester derivative of p-tert-butylcalix[5]arene (1e) toward a variety of butylammonium ions. Semi-empirical AM1 method was used for calculating the binding energies and the formation enthalpies. MM and CVFF forcefields for molecular mechanics calculations were adapted to express the complexation energies of the host. Molecular dynamics were performed to the calculated complex systems to simulate the ionophoric behavior of the host-guest complexes. The absolute Gibbs free energies of the host (1e) complexed with four kinds of butylammonium ions have been calculated using the Finite Difference Thermodynamic Integration (FDTI) method in Discover. Calculation results show that the trend in complex formation is n-$BuNH_3^+$ > iso-$BuNH_3^+$ >> sec-$BuNH_3^+$ > tert-$BuNH_3^+$, which is in good agreement with the experimental results.

• Geomechanics and Engineering
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• v.7 no.6
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• pp.633-647
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• 2014

Biopolymers, polymers produced by living organisms, are used in various fields (e.g., medical, food, cosmetic, medicine) due to their beneficial properties. Recently, biopolymers have been used for control of soil erosion, stabilization of aggregate, and to enhance drilling. However, the inter-particle behavior of such polymers on soil behavior are poorly understood. In this study, an artificial biopolymer (${\beta}$-1,3/1,6-glucan) was used as an engineered soil additive for Korean residual soil (i.e., hwangtoh). The geotechnical behavior of the Korean residual soil, after treatment with ${\beta}$-1,3/1,6-glucan, were measured through a series of laboratory approaches and then analyzed. As the biopolymer content in soil increased, so did its compactibility, Atterberg limits, plasticity index, swelling index, and shear modulus. However, the treatment had no effect on the compressional stiffness of the residual soil, and the polymer induced bio-clogging of the soil's pore spaces while resulting in a decrease in hydraulic conductivity.

• Bulletin of the Korean Chemical Society
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• v.26 no.3
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• pp.447-450
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• 2005

The crude methanol extract of the fruits of Amomum tsao-ko (Zingiberaceae) showed cytotoxic activity. Bioactivity-guided separation led to the isolation of a diarylheptanoid, tsaokoarylone [7-(4-hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)-hepta-4E,6E-dien-3-one] (2). 2 showed cytotoxicity at 4.9 and 11.4 $\mu$g/mL ($IC_{50}$) against human nonsmall cell lung cancer A549 and human melanoma SK-Mel-2, respectively, determined by SRB colorimetric method. During purification-(4-hydroxyphenyl)-4-hydroxyhexan-2-one (4) together with three known diarylheptanoids was also isolated. Their structures were determined from interpretation of spectroscopic data (IR, UV, MS, and NMR) and synthesis confirmed the structure of 2.

• Natural Product Sciences
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• v.19 no.3
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• pp.269-274
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• 2013

Nine terpenoids, spinasterone (1), simiarenol (2), phytol (3), lupeol (4), ${\alpha}$-amyrin (5), $1{\beta},4{\beta}$-dihydroxyeudesman-11-ene (6), 3,7-dihydroxyhumula-4,8(15),10(E)-triene (7), 2,6-dihydroxyhumula-3(12),7(13), 9E-triene (8), 23-hydroxybetulin (9) were isolated from the aerial parts of Aster yomena M. Their structures were identified based on 1D and 2D NMR, including $^1H-^1H$ COSY, HSQC, HMBC and NOESY spectroscopic analyses. Compounds 1 - 9 were isolated from this plant for the first time.

• Proceedings of the Korean Information Science Society Conference
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• 2006.10d
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• pp.586-590
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• 2006

본 논문에서는 다이아몬드 탐색(diamond search, DS)과 효율적인 3 단계 탐색(efficient three-step search, E3SS) 등의 블록 정합 기법(block matching algorithm, BMA)들에서 이용된 작은 다이아몬드(small diamond) 패턴을 광역 탐색에 적합하도록 확장시킨 고속의 움직임 추정 알고리즘을 제안한다. 제안된 알고리즘에서는 탐색 윈도우(search window)의 중앙으로부터 설치된 정사각형 패턴의 크기가 내부에서 대수적으로 감소되며 작은 다이아몬드 탐색(small diamond search, SDS) 기법에 의해 탐색이 완료된다. 실험 결과는 제안된 알고리즘이 DS 보다 평균 3개의 탐색 점을 더 적게 사용하고 E3SS에 비하여 약 5개 정도의 탐색 점 수에 대한 이득을 보이지만 움직임 추정상의 정확도는 다른 고속 BMA들과 거의 동일한 수준을 유지하는 것으로 확인된다.