• Journal of Nutrition and Health
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• 제18권2호
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• pp.147-154
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• 1985

본 실험을 통해 뚜렷한 경향을 보여주는 몇가지 결과를 요약하면 다음과 같다. 식이vitamin E의 과량 첨가는 쥐의 혈청에 반영되어 나타났으며, 식이중의 cholesterol은 이 증가 효과를 감소시키는 경향을 보여주었다. 식이 cholesterol첨가로 인해 쥐의 혈청 cholesterol농도는 영향을 받지 않은 반면, 간의 cholesterol농도는 증가되었다. 과량 vitamin E와 cholesterol 두가지를 식이에 첨가하였을 경우 (IV-ECh IV'-ECh) 간의 cholesterol농도는 식이에 cholesterol만을 첨가한 경우 (III-Ch, III'-Ch)보다 더욱 높게 나타났다. 그리고 vitamin E만을 첨가한 II-E와 II'-E군에서는 간의 cholesterol농도를 낮추어주는 경향을 보였다. 간의 vitamin A 농도는 쥐가 성장함에 따라 감소하였으며 각 실험군 간에는 유의적인 차이를 보이지 않았으나, B군의 경우 과량 vitamin E 식이는 간 vitamin A농도를 감소시키는 경향으로 나타났다. Vitamin E, vitamin A, cholesterol 및 다른 여러 요소들간의 복잡한 상호관계를 알아내기 위해, age가 다른 그리고 여러 species의 동물을 이용한, 장기간의 단계적이고도 체계적인 많은 연구가 요구되어진다.

• Transactions on Electrical and Electronic Materials
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• 제15권3호
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• pp.136-138
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• 2014

In this study, cholesterol sensors consisting of a mixture of cholesterol oxidase (ChOx) and zinc oxide (ZnO) nanoparticles (NPs) are constructed on plastic substrates and their sensing characteristics are examined in air. The current of the ChOx-ZnO NP film decreases in magnitude as cholesterol molecules are adsorbed on the film, due to the resulting increase in the number of electrons generated by the reaction between the cholesterol and the ChOx. The cholesterol sensor shows a high sensitivity of $1.08{\mu}A/mM$ and a wide detection range from 10 nM to 1 mM.

• Journal of Nutrition and Health
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• 제30권6호
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• pp.614-621
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• 1997

The experiments were performed to investigate the effects of protein and protein hydrolysates on lipid metabolism in the hyperlipidemic/hypercholesterolemic rat model induced by feeding fat-enriched diet. In Except 1 male rats were fed four semi-purified high fat and cholesterol diets that contained different nitrogen source, casein(C), casein hydrolysate(CH), corn gluten(G) and corn gluten hydrolysate(GH), for 6 weeks. In Expt. 2 rats were fed high fat and cholesterol diet for 4 weeks to induce hyperlipidemia and hypercholesterolemia. Then the rats were divided into 4 groups and were fed the four kinds of above experimental diets for 4 weeks consecutively. The contents of total lipid , cholesterol and triglyceride in blood, liver and feces were determined. Serum lipid concentrations of CH, G and GH were significantly lower than that of C. Serum cholesterol concentrations of hydrolysate groups(CH and GH) were significantly lower than those of intact protein groups(C and G). Serum HDL -cholesterol concentration tended to increase by hydrolysate intake. The total lipid, cholesterol contents in liver showed similarity results as above. Fecal lipid excretions of CH, G, and GH groups were significantly higher than that of C group. These results indicate that hypolipidemic and /or hypocholesterolemic effect of corn gluten or protein hydrolysates were detected in the process of inducing hyperlipidemia by high-fat and cholesterol diet or after inducing hyperlipidemia.

• Food Science and Biotechnology
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• 제18권4호
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• pp.884-888
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• 2009

Effect of citrus pectin oligosaccharides produced by irradiation was studied on the ability to improve lipid metabolism and hypercholesterolemia in mice fed high cholesterol diets. A total of 35 mice were divided into 5 groups and fed the following diets for 6 weeks: normal diet (C), 0.5% cholesterol (CH), 0.5% cholesterol+5% non-irradiated pectin (P), 0.5% cholesterol+5% irradiated pectin at 20 kGy (PIR), and 0.5% cholesterol+5% irradiated at 20 kGy and dialyzed (PIR-F). CH group had significantly higher serum triglycerides, total cholesterol, and low density lipoprotein (LDL)-cholesterol contents than pectin oligosaccharide-treated groups (p<0.05). Triglycerides and total cholesterol contents was the lowest in C and PIR-F and followed by PIR and P group, and CH group had significantly higher LDL-cholesterol. Serum high density lipoprotein (HDL)-cholesterol content in C group was not different from that in CH and P groups, but lower than that of PIR and PIR-F groups. These results suggest that pectin oligosaccharides produced by irradiation can reduce the levels of serum triglyceride, total cholesterol, and LDL-cholesterol in the blood of mice fed high-cholesterol diets and therefore, irradiation can be used as a tool to produce functional oligosaccharides from citrus pectin.

• 한국식품과학회지
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• 제25권5호
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• pp.571-575
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• 1993

대두 펩타이드의 표면소수도가 혈청 콜레스테롤의 농도에 미치는 영향을 조사하기 위하여, 대두 단백질(ISP), 카세인(CNP), 이들 단백질을 펩신으로 가수분해하여 pH에 따른 펩타이드 침전 획분들(SHT, SH8, SH6, SH4, CHT, CH6, CH5, CH4)을 흰쥐에 섭취시키고 혈청 콜레스테롤 농도 및 분변 스테로이드이 배설량을 측정하였다. 그리고 각 펩타이드의 표면소수도를 ANS 형광법 및 SDS 결합법으로 측정하여, 이들의 상관관계를 분석한 결과, 펩타이드의 ANS 표면소수도가 높아질수록 분변으로 배설된 스테로이드량은 증가하였으며(r=0.81), 혈청 콜레스테롤 농도는 낮아졌다.(r=-0.868). 그러나 SDS 표면소수도는 그들과 상관관계가 없었다. 또한 대두 단백질은 가수분해에 의하여 ANS 표면소수도가 증가하였다. 이상의 결과는 흰쥐의 담즙염이, 소화중 생성된 높은 표면소수도의 펩타이드와 결합하여 체외로 배출되므로서 대두단백질의 섭취에 의한 혈청 콜레스테롤 농도가 낮아짐을 시사하였다.

• 한국식품영양과학회지
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• 제31권5호
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• pp.814-818
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• 2002

배추김치의 dichloromethane(CH$_2$Cl$_2$)획분, ethylacetate (EtOAc) 획분 그리고 물($H_2O$)획분이 1% 콜렛테롤 식이를 16주 동안 섭취한 토끼의 심장, 신장, 그리고 폐의 지질 축적에 미치는 영향에 대한 연구로 100g 식이에 첨가한 김치 용매획분의 양은 8.3mg CH$_2$Cl$_2$, 5.6mg EtOAc 그리고 221.9mg $H_2O$ 획분으로 이 양은 5% 동결건조 김치속에 들어있는 양이다. 토끼는 각 군당 6마리로 한 마리씩 사육하였다. 16주 사육 후 심장, 신장 그리고 폐의 콜레스테롤 및 중성지질의 농도는 대조군에 비해 김치 용매획분에 의해 현저하게 감소하였고, 인지질과 총지방질 함량은 CH$_2$Cl$_2$획분군에서만 유의적으로 감소하였다. (p<0.05). 심장에 있어서 콜레스테롤 농도는 CH$_2$Cl$_2$획분, EtOAc획분 그리고 $H_2O$획분군에서 42%(p<0.05), 21%(p<0.05) 그리고 8%감소하였고, 중성지질 농도는 각 29%(p<0.05), 4%, 그리고 11% 감소하였다. 신장의 경우 콜레스테롤은 CH$_2$Cl$_2$획분, EtOAc획분 그리고 $H_2O$획분군에서 23, 12, 그리고 11% 감소하여 유의적으로 (p<0.05) 억제되었고, 중성지질은 CH$_2$Cl$_2$획분과, $H_2O$획분군에서 각 51%와 21% 감소하여 유의적인 차이를 나타내었다. 폐의 경우 Ch$_2$Cl$_2$획분, EtOAc획분 그리고 $H_2O$획분군에서 콜레스테롤 (-37, -20, 22%) 및 중성지질(-39, -28, -28%) 모두 유의적으로 (p<0.05) 감소하였다. 장기별로 비교해 볼 때 콜레스테롤 함량은 폐, 신장, 심장 순으로 높았고, 중성지방 함량은 심장, 신장, 폐 순으로 높게 나타났다. 인지질 함량은 심장, 신장 그리고 폐에서 비슷하나 수준을 나타냈다. 이러한 결과를 살펴보았을 때 김치용매획분중 CH$_2$Cl$_2$획분에 지질을 감소시키는 활성성분이 가장 많이 함유된 것으로 생각된다.

• 공업화학
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• 제34권6호
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• pp.660-664
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• 2023

In this study, a new system to determine the concentration of cholesterol using a color change was developed. The system comprised diacetylene vesicles and cholesterol oxidase (ChOx). 10,12-Pentacosadiynoic acid (PCDA) was used as the diacetylene compound, and PCDA vesicles were formed using sonication. The H₂O₂ produced during the reaction between cholesterol and ChOx was used to initiate the polymerization of the PCDA in the vesicles. During polymerization, the vesicles changed from colorless to blue. Therefore, the cholesterol concentration was proportional to the intensity of the blue color. The absorption at 665 nm indicated that the blue color was directly proportional to the cholesterol concentration. This indicates that the system can be used for cholesterol detection. The minimum cholesterol concentration detected using this system was 1.0 mM.

• Journal of Nutrition and Health
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• 제26권5호
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• pp.585-592
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• 1993

In order to investigate the hypocholesteremic effect of soybean perptides, soybean protein(ISP), casein(CNP), their peptic hydrolyzates fractionated by acid precipitation at different pH's(SHT, SH8, SH6, SH4, CHT, CH5, CH4) and amino acid mixtures of the same composition as the proteins(SAA, CAA) were fed to rats and the concentration of serum cholesterol was measured. Then in vitro digestibility and molecular weight distribution of the peptides by pepticpancreatic hydrolysis was measured by FPLC. The lower the in vitro digestibility of peptides is, the lower the concentration of serum cholesterol becomes(r=0.986) and the higher the ratio of macropeptides is, the lower the concentration of serum cholesterol becomes(r=-0.932) in rats. These results suggest that the in vitro digestibility of peptides has close relationship to the concentration of serum cholesterol in rats and non-digestible meacropeptides or polypeptides especially more than 1 kDa, formed through digestion in gut, may lower the serum cholesterol in rats.

• 한국미생물·생명공학회지
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• 제20권4호
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• pp.401-408
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• 1992

Cholesterol oxidase를 생산하는 균주를 토양으로 분리하고 이를 배양하여 효소를 생산한 후 정제하여 그 특성을 조사하였다. 본 효소는 CH2 concentrator에 의한 농축, DEAE-cellulose chromatography, Superose 12 column FPLC로 정제하였고, 비활성이 약 31배나 증가하였다. 이 효소는 $50^{\circ}C$, pH 6.0에서 최대 활성을 나타냈고 30-$45^{\circ}C$ 범위에서는 안정하였다. pH에 대한 안정성은 pH 6.0-11.0까지 광범위한 안정성을 보였으며, Km 값은 cholesterol에 대해서 $3.65{\times}10^{-3}$M이며 분자량은 59,500 dalton으로 추정 된다. 또한 $Ca^{2+}$, $Ba^{2+}$, $Fe^{2+}$, Brij 35에 의해서는 효소의 활성이 저해되지 않았지만, $Hg^{2+}$, $Ag^{2+}$ 및 SDS에 의해서는 저해되는 것으로 나타났다.

• Archives of Pharmacal Research
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• 제6권2호
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• pp.115-121
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• 1983

Cholesteryl p-[3-(2-chloroethyl_-3-nitrosoureido] phenylthioaccetate (2) was synthesized : an intermediate, p-[3(2-chloroethyl_-3-nitrosoureido] phenylthioacetic acid (1) is a congener of an antitumor chlorambucil which both the -CH$_{2}$CH$_{2}$-linkage and -N(CH$_{2}$CH$_{2}$Cl)$_{2}$ group of chlorambucil molecule is doubly modified into the respective -S-linkage and -NH-CO-NNO-CH$_{2}$CH$_{2}$CL group. The attackment of cholesterol moiety as a carrier group to p-[3-(2-chloroethyl-3nitrosoureido] phenylthioacetic acid was accomplished through the esterification of cholesterol with p-[3-(2-chloroethyl-3-nitrosoureido] phenylthioacetyl chloride which was obtained from the treatment of p-[3-(2-chloroethyl)-3-nitrosourei-do] phenylthioaceticacid with SOCL$_{2}$, p-[3-(2-chloroethyl)ureido]]-phenythioacetic acid was nitrosated with NaNO$_{2}$ IN 98-100% HCOOH to give exclusively p-[3-(2-chloroethyl_-3-nitrosoureido] phenylthioacetic acid. Antitumor evaluation of compounds, 1 and 2 on L 1210 leukemia did show significant activity (ED$_{40}$ : 1.14.mu./ml and 8.4.mu.g/ml, repectively). Further studies were subjected..