• IMMUNE NETWORK
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• 제6권2호
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• pp.76-85
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• 2006

Background: Chemotaxis is one of the cardinal functions of leukocytes, which enables them to be recruited efficiently to the right place at the right time. Analyzing chemotactic activities is important not only for the study on leukocyte migration but also for many other applications including development of new drugs interfering with the chemotactic process. However, there are many technical limitations in the conventional in vitro chemotaxis assays. Here we applied a new optical assay to investigate chemotactic activities induced in differentiated HL-60 cells. Methods: HL-60 cells were stimulated with 0.8% dimethylformamide (DMF) for 4 days. The cells were analyzed for morphology, flow cytometry as well as chemotactic activities by a time-lapse videomicroscopic assay using a chemotactic microchamber bearing a fibronectin-coated cover slip and an etched silicon chip. Results: Videomicroscopic observation of the real cellular motions in a stable concentration gradient of chemokines demonstrated that HL-60 cells showed chemotaxis to inflammatory chemokines (CCL3, CCL5 and CXCL8) and also a homeostatic chemokine (CXCL12) after DFM-induced differentiation to granulocytic cells. The cells moved randomly at a speed of $6.99{\pm}1.24{\mu}m/min$ (n=100) in the absence of chemokine. Chemokine stimulation induced directional migration of differentiated HL-60 cells, while they still wandered very much and significantly increased the moving speeds. Conclusion: The locomotive patterns of DMF-stimulated HL-60 cells can be analyzed in detail throughout the course of chemotaxis by the use of a time-lapse videomicroscopic assay. DMF-stimulated HL-60 cells may provide a convenient in vitro model for chemotactic studies of neutrophils.

• 한국통신학회논문지
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• 제34권9C호
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• pp.871-879
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• 2009

본 논문에서는 코드분할다중접속(CDMA)시스템의 기지국에 설치할 수 있는 시공간 배열 스마트안테나의 성능이 다중경로 환경의 다양한 파라미터의 변화에 어떻게 반응하는지 살펴보았다. 이 논문에서 사용된 스마트안테나는 공간분산구조로 적응배열안테나를, 시간분산구조로 다중경로의 숫자에 따른 finger 의 레이크 수신기로 구성되었으며 빔형성 방법으로 Decision Directed LMS 방법을 사용한다. 스마트안테나는 다중경로 신호의 입사각분산의 정도와 다중경로(finger)의 숫자에 민감하게 반응하는 한편 지연분산의 정도와는 거의 반응하지 않음이 컴퓨터 모의실험으로 증명되었다. 레이크 수신기의 핑거 숫자의 증가가 스마트안테나의 성능을 비선행적으로 개선시키는 효과를 수반함 역시 모의실험으로 보여졌다. 본 논문의 빔형성 과정에서 가중치벡터의 갱신은 PN상관기가 수신신호를 역확산시킨 후에 이루어진다.

• ETRI Journal
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• 제37권3호
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• pp.450-459
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• 2015

Small cell networks, as an important evolution path for next-generation cellular networks, have drawn much attention. Different from the traditional base stations (BSs) always-on model, we proposed a BSs on-off model, where a new, simple expression for the probabilities of active BSs in a heterogeneous network is derived. This model is more suitable for application in practical networks. Based on this, we develop an analytical framework for the performance evaluation of small cell networks, adopting stochastic geometry theory. We derive the system coverage probability; average energy efficiency (AEE) and average uplink power consumption (AUPC) for different association strategies; maximum biased received power (MaBRP); and minimum association distance (MiAD). It is analytically shown that MaBRP is beneficial for coverage but will have some loss in energy saving. On the contrary, MiAD is not advocated from the point of coverage but is more energy efficient. The simulation results show that the use of range expansion in MaBRP helps to save energy but that this is not so in MiAD. Furthermore, we can achieve an optimal AEE by establishing an appropriate density of small cells.

• IMMUNE NETWORK
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• 제14권6호
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• pp.289-295
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• 2014

Flow cytometric immunophenotyping of peripheral blood lymphocyte subsets is a powerful tool for evaluating cellular immunity and monitoring immune-mediated diseases. The numbers and proportions of blood lymphocyte subsets are influenced by factors such as gender, age, ethnicity, and lifestyle. This study aimed to establish reference ranges for peripheral blood lymphocyte subsets in a healthy Korean population. Blood samples from 294 healthy adults were collected. Lymphocyte subsets were analyzed using a single-platform method with a flow cytometer; white blood cells and lymphocytes were analyzed using an automated hematology analyzer. The mean value of the white blood cell count was $5,665cells/{\mu}l$, and the mean values of the subtype counts (percentages) were as follows: lymphocytes, $1,928cells/{\mu}l$ (35.08%); $CD3^+$ cells, $1,305cells/{\mu}l$ (67.53%); $CD3^+CD4^+$ cells, $787cells/{\mu}l$ (40.55%); $CD3^+CD8^+$ cells, $479cells/{\mu}l$ (25.23%); $CD3^-CD19^+$ cells, $203cells/{\mu}l$ (10.43%); and $CD3^-CD56^+$ cells, $300cells/{\mu}l$ (15.63%). Additionally, the $CD4^+/CD8^+$ ratio was 1.81. In this study, gender and age significantly influenced blood lymphocyte subsets. Our results demonstrate that, as with other populations, a healthy Korean population has its own, region-specific, lymphocyte subset reference ranges.

• Molecules and Cells
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• 제40권7호
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• pp.450-456
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• 2017

Mammalian physiology and behavior are regulated by an internal time-keeping system, referred to as circadian rhythm. The circadian timing system has a hierarchical organization composed of the master clock in the suprachiasmatic nucleus (SCN) and local clocks in extra-SCN brain regions and peripheral organs. The circadian clock molecular mechanism involves a network of transcription-translation feedback loops. In addition to the clinical association between circadian rhythm disruption and mood disorders, recent studies have suggested a molecular link between mood regulation and circadian rhythm. Specifically, genetic deletion of the circadian nuclear receptor Rev-$erb{\alpha}$ induces mania-like behavior caused by increased midbrain dopaminergic (DAergic) tone at dusk. The association between circadian rhythm and emotion-related behaviors can be applied to pathological conditions, including neurodegenerative diseases. In Parkinson's disease (PD), DAergic neurons in the substantia nigra pars compacta progressively degenerate leading to motor dysfunction. Patients with PD also exhibit non-motor symptoms, including sleep disorder and neuropsychiatric disorders. Thus, it is important to understand the mechanisms that link the molecular circadian clock and brain machinery in the regulation of emotional behaviors and related midbrain DAergic neuronal circuits in healthy and pathological states. This review summarizes the current literature regarding the association between circadian rhythm and mood regulation from a chronobiological perspective, and may provide insight into therapeutic approaches to target psychiatric symptoms in neurodegenerative diseases involving circadian rhythm dysfunction.

• 멤브레인
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• 제15권3호
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• pp.198-205
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• 2005

방사능 오염도 측정에 사용하기 위한 이중구조 고분자막이 폴리설폰과 세륨활성화된 이트륨실리케이트(CAYS)를 이용하여 제조되었다. 제조된 막은 순수 고밀도 고분자 지지층과 이에 제막된 고분자 용액의 상전환 공정에 의해 고형화된 CAYS 함침 활성층의 이중구조로 구성된다. 제막공정에서 대기방치 공정이 생략되었을 때 CAYS를 포함하는 활성층은 전형적인 비대칭 구조를 지니며, CAYS 입자들이 고분자 구조 사이에 박혀있는 형상을 지닌다. 제막공정에서 대기에 방치하는 시간이 증가할수록 막의 형상은 스폰지 구조를 띠며 CAYS는 고분자 구조로부터 분리되어 막 내부에 셀 같은 공간에 밀집되어 존재함을 보였다. 한편, 두 충 사의 계면형상은 고분자 고형화 과정에서의 상전환 속도와 밀접한 관련되었으며, 대기방치 시간의 증가에 따라 계면의 구분이 뚜렷하게 나타나지 않았다. 방사능 탐지 특성에서 스폰지 구조를 지니는 막의 고분자 구조는 방사성핵종이 통과할 수 없는 밀집된 형상을 지니면서 탐지효율의 감소를 초래하는 것으로 나타났다.

• IMMUNE NETWORK
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• 제11권6호
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• pp.383-389
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• 2011

Background: EY-6 is one of the newly synthesized indoledione derivatives to induce tumor cell-specific cell death. In this study, we investigated the mechanism of immunological death induced by EY-6 at mouse colon cancer cell as well as at the normal immune cell represented by dendritic cell. Methods: C57BL/6 mouse syngeneic colon cancer cell MC38 was treated with EY-6, and analyzed by MTT for viability test, flow cytometry for confirming surface expressing molecules and ELISA for detection of cytokine secretion. Normal myeloid-dendritic cell (DC) was ex vivo cultured from bone marrow hematopoietic stem cells of C57BL/6 mice with GM-CSF and IL-4 to analyze the DC uptake of dead tumor cells and to observe the effect of EY-6 on the normal DC. Results: EY-6 killed the MC38 tumor cells in a dose dependent manner (25, 50 and $100{\mu}M$) with carleticulin induction. And EY-6 induced the secretion of IFN-${\gamma}$ but not of TNF-${\alpha}$ from the MC38 tumor cells. EY-6 did not kill the ex-vivo cultured DCs at the dose killing tumor cells and did slightly but not significantly induced the DC maturation. The OVA-specific cross-presentation ability of DC was not induced by chemical treatment (both MHC II and MHC I-restricted antigen presentation). Conclusion: Data indicate that the EY-6 induced tumor cell specific and immunological cell death by modulation of tumor cell phenotype and cytokine secretion favoring induction of specific immunity eliminating tumor cells.

• IMMUNE NETWORK
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• 제11권6호
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• pp.342-347
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• 2011

Background: Glial cells are involved in immune and inflammatory responses in the central nervous system (CNS). Glial cells such as microglia and astrocytes also provide structural and functional support for neurons. Migration and morphological changes of CNS cells are associated with their physiological as well as pathological functions. The secreted protein lipocalin-2 (LCN2) has been previously implicated in regulation of diverse cellular processes of glia and neurons, including cell migration and morphology. Methods: Here, we employed a zebrafish model to analyze the role of LCN2 in CNS cell migration and morphology in vivo. In the first part of this study, we examined the indirect effect of LCN2 on cell migration and morphology of microglia, astrocytes, and neurons cultured in vitro. Results: Conditioned media collected from LCN2-treated astrocytes augmented migration of glia and neurons in the Boyden chamber assay. The conditioned media also increased the number of neuronal processes. Next, in order to further understand the role of LCN2 in the CNS in vivo, LCN2 was ectopically expressed in the zebrafish spinal cord. Expression of exogenous LCN2 modulated neuronal cell migration in the spinal cord of zebrafish embryos, supporting the role of LCN2 as a cell migration regulator in the CNS. Conclusion: Thus, LCN2 proteins secreted under diverse conditions may play an important role in CNS immune and inflammatory responses by controlling cell migration and morphology.

• 한국통신학회논문지
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• 제37A권12호
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• pp.1093-1105
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• 2012

본 논문에서는 3-Cell 간섭 환경에서의 하향링크 MU-MIMO를 위한 간섭 공간 재활용 기술 (ISR)과 그 적용방안을 제시한다. 멀티 셀 간섭환경의 셀 경계 사용자들의 안정적인 통신을 위해서는 효율적인 간섭 관리가 필요하다. 하지만 셀 중심부의 사용자들의 경우 간섭의 영향을 적게 받으며 적은 전송 전력으로도 안정적인 통신이 가능하다. 본 논문에서는 셀 경계 사용자의 간섭 공간을 재사용하여 셀 중심부 사용자에게 서비스를 제공하는 ISR 기반 전송 신호 설계 방법을 제시한다. ISR 방식은 간섭정렬(IA)과 결합된 부분적 주파수 재활용 (fractional frequency reuse : FFR) 방식과 비교하여 볼 때 셀 중심 사용자와 셀 경계 사용자에 대한 scheduling에 따라 전체 네트워크 데이터 전송용량 측면에서 20%의 성능 향상을 보이며 또한 셀 중심 사용자의 서비스 품질 (QoS) 요구조건이 고정되어 있는 경우 ISR 방식에 의해 기지국에서의 전송 신호 벡터를 설계함으로서 셀 경계 사용자의 데이터 전송 용량을 증가시킬 수 있다.

• 한국통신학회논문지
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• 제41권9호
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• pp.1001-1009
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• 2016

LTE 시스템에서 단말이 기지국에 데이터를 전송하기 위해서는 기지국이 physical downlink control channel (PDCCH)을 통해 역방향 자원 할당 제어 정보를 방송해야 한다. PDCCH 메시지의 크기 및 전송 횟수는 셀 내 단말의 수에 비례하여 증가하기 때문에 narrowband LTE (NB-LTE) 시스템의 경우 다수의 단말에 의해 발생하는 PDCCH의 오버헤드 증가 문제가 네트워크에 심각한 혼잡을 발생시킬 수 있다. 본 논문에서는 PDCCH의 오버헤드 감소를 위해, 제안하는 간소화 된 PDCCH 정보 비트 구성 방법을 사용하여 PDCCH 메시지의 크기를 줄인다. 또한, PDCCH 메시지의 전송 횟수를 감소시키는 semi-persistent scheduling (SPS) 기법을 NB-LTE 시스템에 적용하여 성능을 평가한다. 모의실험을 통해 SPS의 경우 PDCCH의 오버헤드가 감소하였으며, 시스템의 사용 효율이 높아짐을 보였다.