• 제목/요약/키워드: Cellular Formation

검색결과 925건 처리시간 0.033초

산부인과 펄스형 $CO_2$ 레이저의 펄스 특성에 의한 안정된 동작파형의 시스템 (System of stable action waveform by pulse special quality of obstetrics and gynecology pulse style $CO_2$ laser)

  • 김휘영
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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    • pp.55-57
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    • 2007
  • $CO_2$ laser sees that is most suitable to get this effect through minimum formation damage and advantage that is root enemy of effect that happen in minimum cellular tissue depth of 0.1mm is stable living body organization or internal organs institution. Formation damage by ten can be related in formation's kind or energy density, length of evaporation time. If shorten evaporation time, surroundings cellular thermal damage 200 - because happen within 400ums laser beam in rain focus sacred ground surroundings cellular, tissue without vitiation me by evaporation Poe of very small floor as is clean steam can. Application is possible to vulva cuticle cousins by a paternal aunt quantity, uterine cancer, cuticle tumor by laser system that $CO_2$ laser gets into standard in obstetrics and gynecology application. Because effect that super pulse output is ten enemies of laser if uniformity one pulse durations are short almost is decreased, most of all pulse module special quality of pulse style $CO_2$ laser for obstetrics and gynecology mode stabilization by weight very, in this research to get into short pulse duration and higher frequency density, do switching by high frequency in DC-DC Converter output DC's ripple high frequency to be changed, high frequency done current ripple amount of condenser for output filter greatly reduce can. Ripple of output approximately to Zero realization applying possible inductor realization through a special quality experiment do.

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CSE1L/CAS, a microtubule-associated protein, inhibits taxol (paclitaxel)-induced apoptosis but enhances cancer cell apoptosis induced by various chemotherapeutic drugs

  • Liao, Ching-Fong;Luo, Shue-Fen;Shen, Tzu-Yun;Lin, Chin-Huang;Chien, Jung-Tsun;Du, Shin-Yi;Jiang, Ming-Chung
    • BMB Reports
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    • 제41권3호
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    • pp.210-216
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    • 2008
  • CSE1L/CAS, a microtubule-associated, cellular apoptosis susceptibility protein, is highly expressed in various cancers. Microtubules are the target of paclitaxel-induced apoptosis. We studied the effects of increased or reduced CAS expression on cancer cell apoptosis induced by chemotherapeutic drugs including paclitaxel. Our results showed that CAS overexpression enhanced apoptosis induced by doxorubicin, 5-fluorour-acil, cisplatin, and tamoxifen, but inhibited paclitaxel-induced apoptosis of cancer cells. Reductions in CAS produced opposite results. CAS overexpression enhanced p53 accumulation induced by doxorubicin, 5-fluorouracil, cisplatin, tamoxifen, and etoposide. CAS was associated with $\alpha$-tubulin and $\beta$-tubulin and enhanced the association between $\alpha$-tubulin and $\beta$-tubulin. Paclitaxel can induce G2/M phase cell cycle arrest and microtubule aster formation during apoptosis induction, but CAS overexpression reduced paclitaxel-induced G2/M phase cell cycle arrest and microtubule aster formation. Our results indicate that CAS may play an important role in regulating the cytotoxicities of chemotherapeutic drugs used in cancer chemotherapy against cancer cells.

Purification and Characterization of a Ubiquitin-like System for Autophagosome Formation

  • Bae, Ju-Young;Park, Hyun-Ho
    • Journal of Microbiology and Biotechnology
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    • 제20권12호
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    • pp.1647-1652
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    • 2010
  • Autophagy refers to the bulk degradation of cellular proteins and organelles through an autophagosome and plays a pivotal role in the development, cellular differentiation, aging, and elimination of aberrant structures. A failure of autophagy has been implicated in a growing list of mammalian disease states, including cancer and cardiomyopathy. Two ubiquitin-like systems are highly involved in autophagy, especially in the formation of autophagosomes. Here, we purified and characterized Atg7 (an E1-like enzyme), and Atg3 and Atg10 (E2-like enzymes) in order to gain an insight into the role played by ubiquitin-like systems in the formation of autophagosomes. Interestingly, we observed that Atg7 forms a homodimer to construct an active conformation, unlike other E1-like enzymes. Although Atg3 was detected as a monomer under physiological conditions, Atg10 existed in an oligomeric form, indicating that the mechanism by which Atg10 functions may differ from that of Atg3.

Zinc Deficiency Decreased Alkaline Phosphatase Expression and Bone Matrix Ca Deposits in Osteoblast-like MC3T3-E1 Cells

  • Cho Young-Eon;Lomeda Ria-Ann R.;Kim Yang-Ha;Ryu Sang-Hoon;Choi Je-Yong;Kim Hyo-Jin;Beattie John H.;Kwun In-Sook
    • Nutritional Sciences
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    • 제8권4호
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    • pp.242-249
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    • 2005
  • It is well established that zinc plays an important role in bone metabolism and mineralization. The role of zinc in bone formation is well documented in animal models, but not much reported in cell models. In the present study, we evaluated zinc deficiency effects on osteoblastic cell proliferation, alkaline phosphatase activity and expression, and extracellular matrix bone nodule formation and bone-related gene expression in osteoblastic MC3T3-E1 cells. To deplete cellular zinc, chelexed-FBS and interpermeable zinc chelator TPEN were used. MC3T3-E1 cells were cultured in zinc concentration-dependent (0-15 ${\mu}M\;ZnCl_2$) and time-dependent (0-20 days) manners. MC3T3-E1 cell proliferation by MTT assay was increased as medium zinc level increased (p<0.05). Cellular Ca level and alkaline phosphatase activity were increased as medium zinc level increased (p<0.05). Alkaline phosphatase expression, a marker of commitment to the osteoblast lineage, measured by alkaline phosphatase staining was increased as medium zinc level increased. Extracellular calcium deposits measured by von Kossa staining for nodule formation also appeared higher in Zn+(15 ${\mu}M\;ZnCl_2$) than in Zn-(0 ${\mu}M\;ZnCl_2$). Bone formation marker genes, alkaline phosphatase and osteocalcin, were also expressed higher in Zn+ than in Zn-. The current work supports the beneficial effect of zinc on bone mineralization and bone-related gene expression. The results also promote further study as to the molecular mechanism of zinc deficiency for bone formation and thus facilitate to design preventive strategies for zinc-deficient bone diseases.

예외적 요소와 셀간 이동거리를 최소화할 수 있는 셀 형성과 셀 배치결정 모형 (An integrated model of cell formation and cell layout for minimizing exceptional elements and intercell moving distance)

  • 윤창원;정병희
    • 한국경영과학회:학술대회논문집
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    • 대한산업공학회/한국경영과학회 1996년도 춘계공동학술대회논문집; 공군사관학교, 청주; 26-27 Apr. 1996
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    • pp.121-124
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    • 1996
  • In general, cellular manufacturing system can be constructed by the following two steps. The first step forms machine cells and part families, and the second step determines cell layout based on the result of first step. Cell layout has to be considered when cell is formed becauese the result of cell formation affects it. This paper presents a cell formation algorithm and proposes an integrated mathematical model for cell formation and cell layout. The cell formation algorithm minimizes the number of exceptional element in cellular manufacturing system. New concept for similarity and incapability is introduced, based on machine-operation incidence matrix and part-operation incidence matrix. One is similarity between the machines, the other is similarity between preliminary machine cells and machines. The incapability identifies relations between machine cells and parts. In this procedure, only parts without an exceptional element are assigned to machine cell. Bottleneck parts are considered with cell layout design in an integrated mathematical model. The integrated mathematical model determines cell layout and assigns bottleneck parts to minimize the number of exceptional element and intercell moving distance, based on linearixed 0-1 integer programming. The proposed algorithm is illustrated by using numerical examples.

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The Effects of Glomus etunicatum Innoculation of Robinia pseudoacacia Seedlings on Soil Aggregate Formation in Coal Mine Tailings

  • Hong, Seung-Jin;Park, Yong-Woo;Lim, Kyung-Min;Kim, Se-Kyung;Koo, Chang-Duck
    • 한국환경생태학회지
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    • 제29권1호
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    • pp.88-94
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    • 2015
  • An investigation was conducted on the effects of arbuscular mycorrhizal fungus, Glomus etunicatum on the formation of soil aggregate and mycorhizal development in the roots of Robinia pseudoacacia seedlings in coal mine tailings and forest soil. G. etunicatum formed mycorrhizas by 35.1 % in coal mine tailings and by 48.9 % in forest soil. Its infection was the typical Arum-type forming inter-cellular hyphae and intra-cellular arbuscules. Ergosterol contents were 3.20 ppm in forest soil and 1.92 ppm in coal mine tailings. The formation of soil aggregate per 50 g pot soil was 19.6 g and 9.5 g in inoculated and noninoculated forest soil and 16.5 g and 11.0 g in inoculated and non-inoculated coal min tailings, respectively. In conclusion, G. etunicatum inoculation increased the formation of soil aggregate both in forest soil and coal mine tailings, but was less effective in the latter.

핸드폰의 형상디자인과 표면처리에 대한 연구 (A Study on Surface Treatment and Configuration Design of Cellular Phone)

  • 이정현;박재원
    • 반도체디스플레이기술학회지
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    • 제10권3호
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    • pp.7-13
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    • 2011
  • In Korea, the car mobile phone service had started in 1961 and with this Korea Mobile Telecommunications Services was established in 1984. Its service was mainly focused on the mobile phone service for the car which led the popularity of the full-scale mobile phone service. There are many kinds of materials, colors and patterns which are the elements of surface coating techniques. In light of that, we are focused on the UV coating that is ranked as an important part of the techniques in this study. In the first, the use of various materials for the cellular phone and surface processing techniques for the cellular phone were searched. On the basis of the results, the experiments of actual consumer experience with the exfoliation of the surface were done and through that we proposed the best way of processing the surface for the cellular phone and the materials. The result of the Wear Test showed that thick coating was detached more slowly and each company differed in their results with UV paints despite the same thickness of UV coating. The seal (UV) exfoliation of cellular phone is deeply related with the film thickness and the film formation when the users use the cellular phones and the exfoliation of the cellular phone surface will be decreased if the manufacturers meet the best conditions for the cellular phone.

Piezo-assisted Intracytoplasmic Sperm Injection in Cattle

  • Kim, Se-Woong;Kang, Ho-In;Sung, Ji-Hye;Roh, Sang-Ho
    • 한국수정란이식학회지
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    • 제25권2호
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    • pp.97-101
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    • 2010
  • Intracytoplasmic sperm injection (ICSI) is one of the artificial fertilization methods when only a few sperm are available for insemination, and an important tool for the preservation of genetic materials of endangered animal species, especially the male is infertile. Different from other species such as mice and pigs, the conventional ICSI method which uses spiked pipette for injection (Spike-ICSI) is exhibited low success rates in cattle because the bovinesperm head membrane is hard to break during injection procedure. We chose piezo-assisted ICSI (Piezo-ICSI) for the improvement of the injection procedure including sperm head membrane rupture and efficient puncture of the plasma membrane of the oocytes. In this experiment, we compared the efficacy of the bovine ICSI embryo production between the Piezo-ICSI and Spike-ICSI. The second polar body extrusion, pronuclear formation, cleavage and blastocyst formation were evaluated after implementation of two different ICSI techniques. The Piezo-ICSI tended to show comparably higher rates of the second polar body extrusion (41.7%), the pronuclei formation (42.9%) and the two-cell cleavage (41.4%) than Spike-ICSI does (33.3%, 28.6% and 23.5%, respectively) although there is no statistic significance between two groups. In addition, the blastocysts were only obtained from the Piezo-ICSI group (10.3%). Our finding shows that the Piezo-ICSI may be used as an artificial fertilization method in cattle when in vitro fertilization is not applicable.

셀형유연조립시스템에서의 네트웍형 셀배치 (Network-type Cell Layout in Cellular Flexible Assembly Systems)

  • 노인규;최형호
    • 산업경영시스템학회지
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    • 제19권39호
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    • pp.63-73
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    • 1996
  • With the success of flexible manufacturing systems (FMSs), flexible assembly systems (FASs) have been developed to automate factories further. As in a cellular FMS, a cellular FAS is considered as the most flexible and feasible assembly system configuration Because of the differences between manufacturing and assembly operation, the logic of cell formation and cell layout between a FMS and a FAS is not the same. Since the time for assembly operation is usually relatively short, the transfer time is thus very crucial for the performance of assembly systems. Therefore in assembly systems it is important to reduce the transfer time by sequencing operations efficiently and arranging machines like the sequences. The network-type layout is not only feasible for the machine arrangement based on operation sequences, but it has also layout flexibility. Therefore it is a reasonable layout configuration for cellular FASs. This paper presents a method for the cell layout based on the network-type layout in a cellular FAS design.

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Myosin X and Cytoskeletal Reorganization

  • Ikebe, Mitsuo;Sato, Osamu;Sakai, Tsuyoshi
    • Applied Microscopy
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    • 제48권2호
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    • pp.33-42
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    • 2018
  • Myosin X is one of myosin superfamily members having unique cellular functions on cytoskeletal reorganization. One of the most important cellular functions of myosin X is to facilitate the formation of membrane protrusions. Since membrane protrusions are important factors for diverse cellular motile processes including cell migration, cell invasion, path-finding of the cells, intercellular communications and so on, it has been thought that myosin X plays an important role in various processes that involve cytoskeletal reorganization including cancer progression and development of neuronal diseases. Recent studies have revealed that the unique cellular function of myosin X is closely correlated with its unique structural characteristics and motor properties. Moreover, it is found that the molecular and cellular activities of myosin X are controlled by its specific binding partner. Since recent studies have revealed the presence of various specific binding partners of myosin X, it is anticipated that the structural, biochemical and cell biological understanding of the binding partner dependent regulation of myosin X function can uncover the role of myosin X in diverse cell biological processes and diseases.