• International Journal of Oral Biology
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• v.40 no.1
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• pp.27-33
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• 2015

In the present study, we investigated the effect of staurosporine on the formation of cellular processes in human gingival fibroblasts and rat astrocytes. Staurosporine caused a rapid induction of process formation in human gingival fibroblasts and rat astrocytes in a concentration dependent manner. The process formation of human gingival fibroblasts and rat astrocytes was prevented by the pretreatment with N-acetylcysteine, suggesting that staurosporine-induced ROS production was responsible for the process formation. Colchicine, a microtubule depolymerizing agent, inhibited the staurosporine-induced process formation, whereas cytochalasin D, an actin filament breakdown agent, failed to suppress the formation of cellular processes. This result indicated that polymerization of microtubule, and not actin filament, was responsible for the formation of cellular processes induced by staurosporine. In support of this hypothesis, Western blot analysis was conducted using anti-tubulin antibody, and the results showed that the amount of polymerized microtubule was increased by the treatment with staurosporine while that of depolymerized beta-tubulin in soluble fraction was decreased. These results indicate that staurosporine induces ROS-mediated, microtubule-dependent formation of cellular processes in human gingival fibroblasts and rat astrocytes.

• IE interfaces
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• v.14 no.1
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• pp.20-29
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• 2001

This paper presents a concurrent design approach that deals with manufacturing cell formation and cellular layout in Cellular Manufacturing System. Manufacturing cell formation is to group machines into machine cells dedicated to manufacture of part families, and cellular layout problem determines layout of the manufacturing cells within shop and layout of the machines within a cell. In this paper, a concurrent approach for design of machine cell and cellular layout is developed considering manufacturing parameters such as alternative process plans, alternative machines, production volume and processing time of part, and cost per unit time of operation. A mathematical model which minimizes total cost consisting of machine installation cost, machine operating cost, and intercell and intracell movements cost of part is proposed. A hybrid method based on genetic algorithm is proposed to solve the manufacturing cell formation and cellular layout design problem concurrently. The performance of the hybrid method is examined on several problems.

• Journal of Institute of Control, Robotics and Systems
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• v.3 no.3
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• pp.305-310
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• 1997

In this paper, a fuzzy set based machine-cell formation algorithm for cellular manufacturing is presented. The fuzzy logic is emoloyed to express the degree of appropriateness when alternative machines are specified to process a part shape. For machine grouping, the similarity coefficient based approach is used. The algorithm produces efficient machine cells and part families which maximize the similarity values.

• Journal of the Korea Society of Computer and Information
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• v.21 no.2
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• pp.71-77
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• 2016

There has been unknown polynomial time algorithm for cellular formation problem (CFP) that is one of the NP-hard problem. Therefore metaheuristic method has been applied this problem to obtain approximated solution. This paper shows the existence of polynomial-time heuristic algorithm in CFP. The proposed algorithm performs coarse-grained and fine-grained cell formation process. In coarse-grained cell formation process, the cell can be formed in accordance with machine frequently used that is the number of other products use same machine with special product. As a result, the machine can be assigned to most used cell. In fine-grained process, the product and machine are moved into other cell that has a improved grouping efficiency. For 35 experimental data, this heuristic algorithm performs better grouping efficiency for 12 data than best known of meta-heuristic methods.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.4
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• pp.143-151
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• 1996

The concept of cellular manufacturing is to decompose a manufacturing system into subsystems, which are easier to manage than the entire manufacturing system. The objective of cellular manufacturing is to group parts with similar processing requirements into part families and machines into cells which meet the processing needs of part families assigned to them. This paper presents a methodology for cell formation based on genetic algorithm which produces improved cell formation in terms of total moves, which is a weighted sum of both intercell moves and intracell moves. A sample problem is solved for two, three and four cells with an approach based on genetic algorithms.

• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.705-709
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• 1998

In the course of screening for the substances suppressing bleb formation of K562 cell induced by phorbol 12, 13-dibutyrate (PDBu), an inhibitor, chaetoglobosin A (CgA) was isolated from a cultured broth of unidentified fungus. CgA showed a strong inhibitory activity with the $IC_{50}$ value of 60 pM against bleb formation on K562 cells induced by PDBu, but it did not inhibit the activity of protein kinase C (PKC) in vitro. The inhibitory activity of CgA might be due to the modulation of actin filaments on the cell membrane. CgA exhibited strong cytotoxicity against various human cancer cell lines.

• Journal of the Korean Operations Research and Management Science Society
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• v.22 no.4
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• pp.133-149
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• 1997

The major two steps required to design a cellular layout are cell formation and cell layout. Because of the differences between manufacturing and assembly operations, the logic of cell formation and cell layout between an FMS and an FAS is not the same. Since the time for the assembly operations is usualaly relatively short, the transfer time is thus very crucial for the performance of assembly systems. Transfore in a cellular FAS it is more important to eliminate backtracking operations in assembly planning, not to allow intercellular movements in cell formation, and to arrange machines according to assembly sequence in cell layout. This study presents a method for the integrated layout design in cellular FASs considering the characteristics of FAS, layout, and production factors.

• Industrial Engineering and Management Systems
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• v.1 no.1
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• pp.19-28
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• 2002

An operation sequence of parts is one of the most important production factors in the design of cellular manufacturing systems. Many similarity coefficient method (SCM) based approaches have been proposed to solve cell formation problems in the literature. However, most of them do not consider the operation sequence factor. This study presents an operation sequence ratio (OSR) and modifies some existing similarity coefficients using the OSR to solver cell formation problems considering operation sequences. The computational results show that the OSR ratio is useful and robust in solving cell formation problems with operation sequences.

• BMB Reports
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• v.49 no.8
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• pp.449-454
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• 2016

Stress Granules (SGs) are microscopically visible, phase dense aggregates of translationally stalled messenger ribonucleoprotein (mRNP) complexes formed in response to distinct stress conditions. It is generally considered that SG formation is induced to protect cells from conditions of stress. The precise constituents of SGs and the mechanism through which SGs are dynamically regulated in response to stress are not completely understood. Hence, it is important to identify proteins which regulate SG assembly and disassembly. In the present study, we report Neuregulin-2 (NRG2) as a novel component of SGs; furthermore, depletion of NRG2 potently inhibits SG formation. We also demonstrate that NRG2 specifically localizes to SGs under various stress conditions. Knockdown of NRG2 has no effect on stress-induced polysome disassembly, suggesting that the component does not influence early step of SG formation. It was also observed that reduced expression of NRG2 led to marginal increase in cell survival under arsenite-induced stress.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.1565-1568
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• 1997

In this paper, a fuzzy based machine-cell formation algorithm for cellular manufacturing is presented. The fuzzy lovic is employed to express the degree of appropriateness when alternative machnies are specified to process a part shape. For machine grouping, the similarity coefficient based approach is used. The algorithm produces efficient machine cells and part families which maximize the similarity values.