• Title/Summary/Keyword: Cell yield coefficient

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Ultrasound-Assisted Micellar Extraction for Paclitaxel Purification from Taxus chinensis (Taxus chinensis 유래 파클리탁셀 정제를 위한 초음파를 이용한 마이셀 추출)

  • Park, Ji-Min;Kim, Jin-Hyun
    • Korean Chemical Engineering Research
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    • v.59 no.1
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    • pp.106-111
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    • 2021
  • In this study, an ultrasound-assisted micellar extraction process was developed to efficiently purify the anticancer substance paclitaxel from the plant cell Taxus chinensis. The problem of many extraction steps and long phase separation time in the traditional micellar process could be dramatically improved. The highest paclitaxel yield (~96%, extracted twice) was obtained at 180 W of ultrasonic power and 1.5 h of ultrasonic irradiation time, which was 24.7% higher than that of the traditional method. In addition, the partition coefficient (K) showed a maximum value (24.0) at 180 W of ultrasonic power and 1.5 h of irradiation time. There was no significant difference in the purity of paclitaxel, and the purity of initial paclitaxel (6.81%) increased to 22.0% after purification. Compared to the traditional method, the phase separation time of the back extraction decreased by 40.7-56.2% (ultrasonic power 80 W), 46.3-67.6% (ultrasonic power 180 W), and 51.9-67.6% (ultrasonic power 250 W), respectively. The phase separation time decreased as the ultrasonic power (80-250 W) and irradiation time (0.5-2.5 h) increased.

Recycling Water Treatment of Aquaculture by Using Trickling Filter Process (살수여상공법을 이용한 양어장 순환수처리)

  • KIM Jeong-Sook;LEE Byung-Hun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.2
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    • pp.230-237
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    • 1996
  • The objective of the present study is to evaluate organic removal efficiencies, nitrogen removal efficiencies, kinetic constant, sludge production rates, oxygen requirements, and optimum treatment renditions for recycling water treatment of aquaculture by using a trickling filter process. When the loading rates were $0.500\~0.082kg\;COD/m^3/day$ and $0.271\~0.044kg\;NH_4^+-N/m^3/day$, SCOD and ammonia removal efficiencies were $74.5\~84.0\%$ and $43.7\~61.8\%$, respectively. The maximum removal rate of ammonia was 119.5 mg/L/day. Observed cell yield coefficient in the trickling filter reactor was 0.572 kg VSS/kg $BOD_{rem}$. When the hydraulic loading rate was $6.712\~40.341m^3/m^2/day$, oxygen uptake rate was $1.33\~7.22\;mg\;O_2/L/hr$.

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Manufacturing of Korean Paper(Hanji) with Indian Mallow (Abutilon avicennae Gaertner) as the Alternative Fiber Resources(I) - Productivity and Pulping Characteristics of Indian Mallow - (대용섬유자원으로써 어저귀를 이용한 한지제조(제1보) - 어저귀의 건물 생산량 및 펄프화 특성 -)

  • Jeong, Seon-Hwa;Cho, Nam-Seok;Choi, Tae-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.2
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    • pp.144-150
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    • 2002
  • Indian mallow is characterized by the rapid growth and high harvest of the fibrous materials. This study was carried out to investigate the sheet properties of Hanji using Indian mallow, made by different pulping methods, such as alkali and sulfomethylated pulpings and different stock compositions, various mixing ratios of bast fiber, woody core and whole stalk fibers. The results from this study were summarized as follows. The length and width of the bast fibers were 1.40-430 mm(av. 235 mm), and 9.2-26.4 ㎛(av.18.3 ㎛), respectively. The cell wall thickness was 4.0-115 ㎛(av. 7.7 ㎛). Runkel ratio, flexibility coefficient and fiber length/fiber width ratio were 1.38, 0.42 and 128 respectively. Bast fiber and whole stalk were cooked by alkali and sulfomethylated methods. Sulfomethylated pulping resulted in superior pulp in terms of yield and quality as compared with those of alkali pulping. The pulp yields of bast fiber was higher than those of whole stalk

Simultaneous Treatment of Carbon Dioxide and Ammonia by Microalgal Culture (조류배양을 통한 이산화탄소 및 암모니아의 동시처리)

  • ;;Bohumil Volesky
    • KSBB Journal
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    • v.14 no.3
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    • pp.328-336
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    • 1999
  • A green microalga, Chlorella vulgaris UTX 259, was cultivated in a bench-scale raceway pond. During the culture, 15%(v/v) $CO_2$ was supplied and industrial wastewater discharged from a steel-making plant was used as a culture medium. In a small scale culture bottle, the microalga grew up to 1.8 g $dm^{-3}$ of cell concentration and ammonia was completely removed from the wastewater with an yield coefficient of 25.7 g dry cell weight $g^{-1}\;NH_3-N$. During the bottle-culture, microalga was dominant over heterotrophic microorganisms in the culture medium. Therefore, the amount of carbon dioxide fixation could be estimated from the change of dry cell weight. In a semi-continuous operation of raceway pond with intermittent lighting (12 h light and 12 h dark), increase of dilution rate resulted in increase of the ammonia removal rate as well as the $CO_2$ fixation rate but the ammonia removal efficiency decreased. Ammonia was not completely removed from the medium (wastewater) of raceway pond which was operated in a batch mode under a light intensity up to 20 klux. The incomplete removal of ammonia was believed due to insufficient light supply. A mathematical model, capable of predicting experimental data, was developed in order to simulate the performance of the raceway pond under the light intensity of sun during a bright daytime. Simulation results showed that the rates of $CO_2$ fixation and ammonia removal could be enhanced by increasing light intensity. According to the simulation, 80 mg $dm^{-3}$ of ammonia in the medium could be completely removed if the light intensity was over 60 klux with a continuous lighting. Under the optimal operating condition determined by the simulation, the rates of carbon dioxide fixation and ammonia removal in the outdoor operation of raceway pond were estimated as high as $24.7 g m^{-2} day^{-1}$ and $0.52 g NH_3-N m^{-2} day^{-1}$, respectively.

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