• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.47-53
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• 1997

Appropriation of partial substitution of concentrate mixture by urea-molasses-mineral block (UMMB) lick supplements for 20 lactating crossbred cows in 2nd and 3nd lactation was studied. Animals fed on wheat straw ad lib. and Berseem (Trifolium alaxandrium) fodder @ 1.5 kg/d on dry matter basis. Animals of control group were given concentrate supplement, while in treatment groups 10% of the concentrate requirement was substituted with 3 different types of UMMB lick type A ($T_1$), type B ($T_2$) and type C ($T_3$). CP content of the ration was 15%. Total dry matter intake (DMI) was about 1.0 kg/kg of fat corrected milk (FCM) yield and was not significantly different between control and treatment groups. Digestibility of neither proximate principles nor cell wall constituents were deviated on UMMB licks partial supplementation. FCM yield was increased by 140, 410 and 460 g/d, in $T_1$, $T_2$ and $T_3$, respectively, in comparison to control group but differences were statistically invalid. Though fat per cent was reduced, fat yields were remain constant among treatments. Milk composition was unaltered except significant difference (p < 0.01) in non-protein nitrogen (NPN) content. Gross-N and digestible-N conversion was significantly higher (p < 0.01) with $T_1$, $T_2$ and $T_3$ than control group. Energy utilization efficiency for milk production was only 36%. Result demonstrated that UMMB licks could be partial supplemented up to 10% of the concentrate requirement of crossbred cows yielding on an average 14kg/d without any adverse effect on feed intake, nutrient utilization and mild production. Comparatively, UMMB lick type B and C was proved better than type A and also economically viable.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.133-137
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• 1996

Duckweed, an aquatic plant of the family Lemnaceae, is a rich source of protein and also contains cell wall materials. Spirodela, Lemna and Wolffia, the most available species of duckweeds were evaluated in terms of their chemical composition, the rate and extent of digestion of their dry matter(DM) and crude protein(CP) in the rumen and also their acceptability to the cattle. The three species contained CP of 284, 399 and $299g{\cdot}kg^{-1}$ DM, respectively; NDF of 471, 574 and $476g{\cdot}kg^{-1}$ DM, respectively; ADF of 215, 203 and $227g{\cdot}kg^{-1}$ DM, respectively. The rumen digestibilities of DM of the three species for 24 h were 410, 570 and $731g{\cdot}kg^{-1}$ DM, respectively and of CP were 528, 740 and $778g{\cdot}kg^{-1}$ DM, respectively. The rates of digestion of DM of the three duckweeds were 2.22, 3.63 and $5.73%h^{-1}$, respectively and of CP were 5.14, 4.22 and $6.05%h^{-1}$, respectively. Similarly, the extent of digestion of DM were 853, 723 and $926g{\cdot}kg^{-1}$ DM, respectively and of CP were 801, 874 and $943g{\cdot}kg^{-1}$ DM, respectively. Mixed duckweeds as a component of a concentrate mixture were eaten by the cattle at the rate of 10% of their live weights. It may be concluded that the dry matter and crude protein of the available duckweeds wee highly degradable in the rumen and may be fed to cattle mixing with concentrates. For the effective utilization of duck weeds as cattle feed their effect on the rumen digestion kinetics of a roughage diet need to be studied carefully.

• Journal of Microbiology and Biotechnology
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• v.21 no.11
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• pp.1174-1178
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• 2011

Exopolysaccharides (EPSs) are microbial polysaccharides that are released outside of the bacterial cell wall. There have been few studies on EPS-producing lactic acid bacteria that can enhance macrophage activity and the underlying signaling mechanism for cytokine expression. In the current study, EPS-overproducing Lactobacillus (L.) paracasei KB28 was isolated from kimchi and cultivated in conditioned media containing glucose, sucrose, and lactose. The whole bacterial cells were obtained with their EPS being attached, and the cytokine-inducing activities of these cells were investigated. Gas chromatography analysis showed the presence of glucose, galactose, mannose, xylose, arabinose, and rhamnose in EPS composition. EPS-producing L. paracasei KB28 induced the expression of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6, and IL-12 in mouse macrophages. This strain also caused the degradation of $I{\kappa}B{\alpha}$ and phosphorylation of the major MAPKs: Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK)1/2. The use of pharmacological inhibitors showed that different signaling pathways were involved in the induction of TNF-${\alpha}$, IL-6 and IL-12 by L. paracasei KB28. Our results provide information for a better understanding of the molecular mechanisms of the immunomodulatory effect of food-derived EPS-producing lactic acid bacteria.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.148-157
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• 2020

Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.3
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• pp.339-345
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• 1988

Four actinomycetes which produces extracellular chitinase were isolated from soil and organic matter all over the Chonnam provincial area. The chemical composition, morphological, cultural and physiological properties of isolated strain S-25, S-42, S-172 and S-267 were studied in relation to the toxonomical properties. All of strains contained phospholipids such as phosphatidyl ethanolamine, phosphatidyl inositol and diphosphatidyl glycerol. The components of cell wall in all strains have L, L-DAP, Glutamic acid, Alanine, Glycine and Glucosamine. The surface of spore is smooth and colony is grey in all strains, Based on the results obtained in these experiments all of strains are identified as Streptomyces sp.S-25, Streptomyces sp.S-42, Streptomyces sp.S-l72 and Streptomyces sp. S-267.

• Journal of the Korean Wood Science and Technology
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• v.30 no.4
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• pp.1-7
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• 2002

This study deals with physical properties of Larch(Larix kaemferi Carr.) treated by steaming at temperatures above 100℃. Treatment conditions of this experiment were operated at regular intervals of 10℃ at temperature up to 130℃ for 10, 30 and 60 minutes by using the auto-clave. The Bending strength and compressive strength along the grain were slightly decreased with increasing the treating temperatures. There was no significant relations between wood strength and steaming temperatures. At the range from 120℃ to 130℃ of steaming temperature, it was considered that the change of composition and structure in cell wall was changed due to release of stresses. Water absorption characteristics were not affected by steaming temperature. In this experiment limits, it was concluded that physical properties of wood by steaming treatment were affected more largely by wood density than by treating temperature.

• Food Science of Animal Resources
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• v.29 no.5
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• pp.619-626
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• 2009

As a trial for the development of a new starter culture for yogurt products, more than two hundred lactic acid bacteria strains were isolated from raw milk and healthy human feces. The strains that showed excellent growth and acid production ability in the 10% skim milk media were selected and identified as Lactobacillus casei through the API carbohydrate fermentation pattern and 16S rDNA sequence analysis. L. casei CU2604 was further investigated for its physiological characteristics as a starter culture compared with a commercial strain. The CU2604 strain showed good acid production and growth characteristics in milk, which were comparable to those of the L. casei Shirota strain. Despite the fact that both these strains displayed the same sugar fermenting pattern and PFGE band pattern, and had similar growth characteristic in milk, L. casei CU2604 exhibited different fatty acid composition in the cell wall, showed more tolerance to bile and to pH, and presented better growth inhibition activity against pathogenic bacteria. Based on these results, the L. casei CU2604 strain holds great promise for use as a novel and efficient starter culture in the production of yogurt. Additional studies on the probiotic characteristics of this strain are currently being conducted.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1265-1265
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• 2001

The organic materials included in excreta of livestock are important resources for organic manure and for improving soil quality, although there is still far from effective using. One reason for this is still unclearly standard of quality for evaluation of manure made from excreta of livestock. Therefore, the objective of this study is to develop rapid and accurate analytical method for analyzing organic compositions of manure made from excreta of livestock, and to establish quality evaluation method based on the compositions predicted by near infrared reflectance spectroscopy (NIRS). Sixteen samples of manure, each eight samples prepared from two treatments, were used in this study. The manure samples were prepared by mixing 560 kg feces of swine,60 kg sawdust with moisture content was adjusted to be 65%. The mixture was then keep under two kinds of shelter, black and clear sheets, as a treatment on the effect of sunlight. Samples were taken in every week (form week-0 to 7) during the process of manure making. Samples were analyzed to determine neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) by detergent methods, and organic cell wall (OCW) and fibrous content of low digestibility in OCW (Ob) by enzymatic methods. Biological oxygen demand (BOD) was analyzed by coulometric respirometer method. These compositions were carbohydrateds and lignin that were hardly digested. Spectra of samples were scanned by NIR instrument model 6500 (Pacific Scientific) and read over the range of wavelength between 400 and 2500nm. Calibration equations were developed using eight manure samples collected from black sheet shelter, while prediction was conducted to the other eight samples from clear sheet shelter. Accuracy of NTRS prediction was evaluated by correlation coefficients (r), standard error of prediction (SEP) and ration of standard deviation of reference data in prediction sample set to SEP (RPD). The r, SEP and RPD value of forage were 0.99, 0.69 and 7.6 for ADL, 0.96, 1.03 and 4.1 for NDF, 0.98, 0.60 and 4.9 for ADF, 0.92, 1.24 and 2.6 for Ob, and 0.91, 1.02 and 7.3 for BOD, respectively. The results indicated that NIRS could be used to measure the organic composition of forage used in manure samples.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1303-1308
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• 2003

Samples of eleven brown midrib (ICSU 96031, ICSU 93046, ICSU 96082, ICSU 96078, ICSU 96075, ICSU 95101, ICSU 96034, ICSU 96063, ICSU 45116, ICSA 93-3 and ICSA 3845 X 3816) and nine white midrib genotypes (ICSU 96050, ICSU 96030, ISU 95082, SSG 59-3, FSHI 93-1, FSHI 2219A X 3211, HC 171, ICSA 93-2 and ICSA 93-1) based on their phenotypic appearance were collected at 50 per cent flowering from the sorghum germplasm grown at Research farm of IGFRI, Jhansi. These genotypes were evaluated with respect to crude protein, fiber composition, in-sacco dry matter, OM, cell wall components disappearance/digestibility besides the fodder yield, total phenolic and availability index values. Brown midrib genotypes were lower (p<0.05) in NDF, ADF, cellulose and acid detergent lignin concentration than white midrib genotypes. Mean NDF, ADF, cellulose and lignin contents were 69.4, 42.1, 35.4 and 5.7% in brown mid rib vis-a vis 75.8, 47.5, 39.6 and 7.3% in white mid rib genotypes. Nonsignificant (p>0.05) differences were observed in dry matter, crude protein and organic matter contents between brown midrib and white midrib genotypes. Phenolic contents were significantly (p<0.05) lower in browm mid rib (0.2) than white mid rib (0.3%) sorghum. Brown midrib genotypes exhibited significantly (p<0.05) higher in-sacco DM, OM and CP disappearance than normal (white midrib) genotypes. The mean degradability of DM, OM and CP was 64.1, 62.6 and 79.6% in brown mid rib and 53.1, 54.0 and 76.6% in white mid rib genotypes, respectively. There were no significant (p>0.05) differences between genotypes in extent of fiber fraction degradability though in-sacco NDF and ADF degradability was more by 5 and 4 units, respectively in brown midrib genotypes vis-a-vis white midrib genotypes. Average fodder yield (green and dry g/plant) and availability index (%) values were significantly (p<0.05) higher for brown midrib (474.2, 129.8 and 80.4) genotypes than white midrib (375.0, 104.8 and 69.2) genotypes. Lignin contents had significant negative correlation with DM, OM, NDF and ADF degradability. The results of the study revealed that brown midrib genotypes are superior not only with regard to chemical entities and disappearance of DM and fiber fractions but also better in respect of fodder yield and availability index values. Thus, brown midrib sorghum strains may be useful in increasing digestibility, intake, feed efficiency and animal performance.

• Applied Biological Chemistry
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• v.34 no.1
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• pp.54-60
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• 1991

Serratia marcescens CK-3, decomposing chitin which is a mar component of cell wall in phyitopathogenic fungi, was isolated from the continuous cropping rhizosphere of pepper and cucumber and its enzymatic property was examined. S. marcescens CK-3 was found tn have an tagonistic effects against, Fusarium axysporum and Rhizoctonia solani and to have complex enzyme system such as chitinase, laminarinase, and proteinase. The preferable composition of the medium for production of chitinase was fond and was as follows : colloidal chitin 1.5%, tryptone 0.5%, glucose 1.0%, peptone 0.2%, $MgSO_4{\cdot}7H_2O\;0.1%,\;K_2HPO_4\;0.1%,\;and\;NaCl\;0.1%$(w/v), pH 6.8. The maximum enzyme production was observed after culture of 72 hours at $30^{\circ}C$ using a medium containing the above chemical composition. The optimal pH and temperature for in vitro activity of chitinase from S. marcescens CK-3 were pH 7.5 and $50^{\circ}C$, respectively. The enzyme activity in-creased by metal ions such as$Ag^+$ and $Mn^{++}$.