• Journal of the Korean Wood Science and Technology
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• 제9권1호
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• pp.1-12
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• 1981

The structure of spiral thickenings, particulary the appearance, arrangement and orientation of thickenings in compression wood of Torreya nucifera, were studied in detail by light and polarizing microscope, scanning and transmission electron microscope. The results obtained are as follows: (1) Using the inclined sections at an angle of 45 degrees to the fiber axis, it seems that we can not only observe the more accurate transverse view of the thickenings but also investigate the formation of their thickenings. (2) Generally 2-4 pieces of thickenings are projected to the cell lumen as nipple-like appearance in transverse section and are as frequent, well developed, forming pair and have the rope-like appearance in radial surface. (3) The secondary wall of early wood is composed of 3 layers (S1, S2, S3) and orientation of thickening appears S helix but that of late wood is of 2 layers (S1, S2) and that orientation shows Z helix. Above two regions are demaracted at several tracheid cells from the growth ring boundary. (4) Orientation of thickening seems to be a element showing the characteristics of compression wood in Torreya nucifera. (5) It believes that the thickenings of compression wood are integral part of the S3 in early wood tracheids and of the S2 in late wood and have the same orientations as the inner-most microfibrils in these layers. (6) Thickening and cavities seem to be not formed together in a secondary cell wall of same tracheids.

• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.878-884
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• 2020

Penicillium digitatum and P. italicum are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, p-anisaldehyde exhibited a strong inhibitory effect on P. digitatum and P. italicum, with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.00 μl/ml. Additionally, p-anisaldehyde visibly inhibited both the green mold and blue mold development of citrus fruits inoculated with P. digitatum and P. italicum. The mycelia morphologies of these pathogens were greatly altered, and the membrane permeability and cell wall integrity of mycelia were severely disrupted under p-anisaldehyde treatment. These results suggest that the antifungal activity of p-anisaldehyde against P. digitatum and P. italicum can be attributed to the disruption of the cell wall integrity.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.366-370
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• 1999

Samples of whole plant, leaf and stem of Akbar, Neelum, UM-81 and lZ-31 cultivars of maize fodder harvested up to 14 weeks at different growth stages were drawn and analysed for dry matter contents and various cell wall constituents such as NDF, ADF, hemicellulose, cellulose, lignin, cutin and silica. The dry matter contents of whole maize plant, leaf and stem increased significantly (p<0.01) with advancing plant age. Maximum dry matter was found in the leaf fraction of the plant. The cell wall components continued to increase significantly (p<0.001) in whole maize plant and its morphological fractions as the age advanced. Maximum values for NDF, ADF, cellulose and lignin were observed in stem followed by whole plant and leaf, whereas hemicellulose, cutin and silica contents were higher in leaf fraction of the plant. The cultivars were observed to have some effects on chemical composition of all plant fraction. The results indicated that maturity had a much greater effect on the concentration of all the structural components than did the cultivars. It was concluded that maize fodder should be cut preferably between 8th to 9th week of age (flowering stage) to obtain more nutritious and digestible feed for livestock. Among the maize cultivars, Neelum proved to be the best, due to its higher dry matter contents and lower lignin concentration.

• Journal of Ginseng Research
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• 제21권2호
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• pp.91-97
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• 1997

In order to elucidate the mechanism of red-colored phenomena(RCP) in ginseng(Panax ginseng C.A. Meyer), distribution of inorganic elements of ginseng root and its surrounding soil, and microflora in the soil were investigated. Red brown colored-substances were accumulated in the cell wall of epidermis at early stage of red-colored ginseng (RCG). Cell wall of the late stage of RCG was disordered and microorganisms were shown in the disordered cell wall. Al, Si and Fe contents among inorpanic elements in the epidermis of RCG were higher at two or three times than that of healthy ginseng. On the other hand, K content was higher at three times in healthy ginseng than that of RCG. Especially, Fe content was higher at three times in lateral roots of RCG than that of healthy ginseng. Total 21 strains of microorganisms were isolated on the 523 medium from surface soil, surrounding soil of both healthy and RCG, and RCG. Six strains of microorganisms among them were resistant to 2 mM Fe. Two species in Bacillus app. and Lactobacillus app. , and one species in Micrococcus sp. and Npisseria sp. respectively were identified. It seemed that RCP was closely related with the distribution and uptake of inorganic elements, was also correlated Fe-independent metabolism of microorganisms.

• Journal of Forest and Environmental Science
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• 제30권2호
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• pp.226-232
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• 2014

To understand transition characteristics from wood to charcoal the dimensional changes of carbonized woods at low temperature from $300^{\circ}C$ to $350^{\circ}C$ at the intervals of $10^{\circ}C$ were investigated. Three species of hardwoods and two species of softwoods were used in this study. Measurements of dimensional changes of cells were observed by stereoscopic microscope and an image analyzer. The apparent volume of each specimen decreased greatly with increasing temperature. Severe cracks and collapse were observed frequently in hardwoods and hardly in softwoods. Vessel diameter and tracheid cell wall thickness of the wood samples were decreased with increasing carbonization temperature. Contraction of vessel diameter in tangential direction was greater than that in radial direction. Cell wall thickness of tracheids decreased with increasing carbonization temperature. Consequently, even though it was small range of carbonization temperature, dimensions of wood components were changed considerably.

• Journal of Ginseng Research
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• 제16권1호
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• pp.37-43
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• 1992

Structural changes of the endosperm of Panax ginseng C.A. Meyer from fertilization to germination were investigated by light microscope. The endosperm of the ginseng seed is cellular type. Since endosperm cells adjacent embryo continuously breakdown and disappear with the elongation of embryo, the real of endosperm is gradually decreased. As the anatropous ovules of immature seed with green seed coat developes more and more, ovary cells adjacent ovary cavity become abundant by the periclinal division, their size is decreased, hypotrophy of cell wall discern, and they are gradually differentiated in seed coat. Though embryo responds strongly to basic dye at the stage of completion of endosperm formation, tissue of endosperm responds to acidic dye positively Cell wall of embryo and endosperm are composed of primary cell wall not lignified. Endosperm cells adjacent embryo begin to breakdown in the endosperm tissue of indehiscent seed before the beginning of the after-ripening. Dehiscent seed of which seed coat is opened through after-ripening represent the form as a seedling in the result of embryo developments with the formation of organs; radicle, cotyledon, plumule. Umbilifom layer represents strong positive response to the toluidine blue and the basic function. Umbiliform layer that endosperm cells breakdown and disappear is observed clearly at the periphery of the embryo cotylemon, while slightly at the periphery of the radicle.

• Journal of Plant Biology
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• 제34권4호
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• pp.261-266
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• 1991

The hairy root was induced form potato tuber disc by infection of a. rhizogenes. The detection of the agropine and mannopine by paper electrophoresis confirmed that induced hairy root was transformed by A. rhizogenes. The activity of peroxidase was the highest at 5 weeks and isozyme pattern of peroxidase revealed 3 cathodic bands and 2 anodic bands and new C4 band(pI 4.6) was observed at 7 weeks after cultivation in hairy root was isoelectric focusing. To study the effect of Ca2+ on cell wall formation in hairy root, channel blocker of Ca2+ was treated. The activity of $\beta$-glucan synthetase II(GS II) related to cell wall synthesis was inhibited by about 50% in diltiazem and flunarizine treatment than that of control, but stimulated in CaCl2 treatment. Therefore these results showed that Ca2+ might be an effective factor in the cell wall formation. The activity of GS II by NaF treatment was increased by about 30%. This result suggested that the activity GS II is changed through phosphorylation process.

• KSBB Journal
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• 제7권3호
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• pp.209-215
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• 1992

당근 현탁배양세포에서 세포벽 합성 효소인 GSII의 활성에 다가양이온의 작용기작을 조사하였다. 다가양이온의 특성을 가진 poly-L-Iysine과 poly-L-ornithine은 GSII의 활성을 40~50% 정도 촉진 시켰으며, ATP도 NaF도 이와 비슷한 결과를 나타냈다. 한편 poly-L-Iysine과 poly-L-ornithine은 막투과성에는 영향을 미치지 못하였으며, protein kinase의 활성제인 TPA는 GSII의 활성을 대조구에 비해 35%정도 증가시켰고 억제제인 H-7은 30%정도 감소시켰다, 이러한 결과들은 다가양이온이 인산화 과정을 통해 GSII의 활성을 증가시켜 세포벽의 합성을 증진시키리라 사료된다.

• 한국의류산업학회지
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• 제11권2호
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• pp.337-343
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• 2009

This study systematically investigated a method for extraction of safflower (Carthamus tinctorius Linnaeus) colorants by ultrasonic treatment. Compared to pigments productivity and cell wall structures of safflower after general and ultrasonic method, the ultrasonic method showed high extraction efficiency of safflower pigments due to destruction of safflower cell wall caused by high vibration energies. Microscopic analysis confirmed the hypothesis that the ultrasonic treatment of safflower caused its cell wall structure loosened and made efficient extraction of safflower pigments. And also, LC-MS/MS analysis revealed that productivities of the yellow and red safflower pigments by ultrasonic method were 21.9% and 14.6% higher, respectively, than those of pigments extracted by general method. The ultrasonic extracted yellow and red colorants could be used to dye not only natural fibers like cotton, silk and wool, but also synthetic fiber like nylon, and generally gave a better color tone than the general extracted colorants from safflower due to the affinities of red and yellow colorant on different fibers. As the yellow and red colorant were extracted by ultrasonic treatment in water, the K/S value on of 550/440nm of cotton and rayon was increased but in the case of silk and wool the change of this value was almost not detected. Finally, this technique might provide a solution to establish reproducibility and standardization for the extraction and dyeing methods on fabrics.

• ALGAE
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• 제23권3호
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• pp.163-175
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• 2008

Phenolic compounds play a major role in the interaction of plants with their environment. They are thought to have been a feature of higher plants since early colonization of the land. Phenolics are crucial for many important aspects of plant life. They can play structural roles in different supporting or protective tissues, for example in cell walls, they can be involved in defence strategies, and signalling properties particularly in the interactions between plants and their environment. In brown algae, phenolic compounds are contained within membrane bound vesicles known as physodes, and their roles in algae are thought to be similar to those of higher plant phenolics. They can be stained using various histochemical stains, however, none of these stains are phenolic specific so care must be taken during interpretation of such results. Many, but not all phenolics are also autofluorescent under UV or violet light. Physodes are involved in cell wall construction, both in primary and secondary walls in brown algae. They bind together with other wall components to make a tough wall. They have also been found to play a role at fertilization, in blocking polyspermy in some species. Sperm are very quickly rendered immobile after phenolic release from newly fertilized zygotes seconds after fertilization. Phenolic compounds are thought to be important herbivore deterrents in some species due to their astringent nature. Phenolic compounds also offer effective UV protection in the early life stages and also the adults of many algal species. In the future, this factor may also make them an important player in the pharmaceutical and skincare industries.