• 대한한방소아과학회지
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• 제22권1호
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• pp.123-147
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• 2008

Objectives This study is to investigate how dose Ikhwang-San can be effective on SD rats which deteriorated immunity caused by methotrexate. Methods The test sample were dosed once a day for 14 days by gastric gavage at the beginning of dosage 1000, 500 and 250㎎/㎏/10㎖ from 2 days after last MTX-dosing, and the changes of the body and spleen weight, total number of blood leukocytes, total number of lymphocytes, the percentage of B-cell, T-cell, CD3+CD4+ T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the blood and spleen were observed. Results The changes of the body and spleen weight, the total number of blood leukocytes, the total number of lymphocyte in the blood and spleen were significantly increased in IHS Extracts groups comparing with the control group. The percentage of B-cell, T-cell, CD3+CD4+ T-cell in the blood and spleen were significantly increase in IHS groups and comparing with the control group. The ratio of CD4+/CD8+ T-cell in blood and spleen was significantly increased in IHS Extracts groups comparing with the control group. Conclusions According to those results, Ikhwang-San has good immunostimulating effect on SD rats which had deteriorated immunity caused by methotrexate.

• Animal cells and systems
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• 제1권1호
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• pp.107-113
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• 1997

Antisera against the myotropic neuropeptide leucokinin I, originally isolated from head extracts of the cockroach Leucophaea maderae, have been used to investigate the distribution of the leucokinin I-immunoreactive (LK I-IR) neurons in the brain of the common cutworm, Spodoptera Iitura, during postembryonic development. The LK I-IR neurons are found at the larval stages (excluding first instar larval stage), pupal stages, and adult stage, of which the brains have been examined in this experiment. The number of the LK I-IR neurons in the brain increases from the second instar larva to the fifth instar larva which has about 32, the largest number in all postembryonic stages. Thereafter, the LK I-IR neurons begin to decrease in number. During the pupal stages, smaller number of LK I-IR neurons persist in the brains; 6 or 4. At adult stage the brain contains 8 LK I-IR neurons. The LK I-IR cell bodies are distributed in each dorsal cortex of both cerebral hemispheres in the second instar larva and through all the neuromeres of the brain during later larval stages, despite of being a large number of the LK I-IR cell bodies in dorsolateral neuromeres. At pupal stages, most of the LK I-IR cell bodies are found in the pars intercerebralis. Extremely small number of the LK I-IR cell bodies are localized in the pars lateral is. Adult brain contains the LK I-IR cell bodies in the pars intercerebralis and the middle cortex of the posterior brain. The LK I-IR nerve processes can be easily found in the neuropils of almost all the neuromeres in the brains of third, fourth, fifth and sixth instar larvae. Most of the LK I-IR nerve fibers in those brains are originated from the LK I-IR cell bodies located in the brains. The LK I-IR cell bodies which have very weak reactivities to the antisera do not show projection of the LK I-IR nerve processes in the brains.

• 대한한방소아과학회지
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• 제23권2호
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• pp.51-85
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• 2009

Objectives : The purpose of this study is to investigate the effect of concurrent administration of KKSDU and AJ on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the condition in humans. Methods : We evaluated clinical skin score, hematology, serum total IgE and IgG1 of NC/Nga atopic dermatitis mouse and analyzed the cytoline level, total cell number, immunohistochemical staining, histological features of axillary lymph node(ALN), draining lymph node(DLN), peripheral blood mononuclear cells(PBMCs) and dorsal skin tissue in NC/Nga mouse. Results : Orally administration of KKSDU and concurrent administration of KKSDU and AJ decreased the clinical skin score, total cell number of WBC, platelet, neutrophils, eosinophils in blood, serum total IgE & IgG1, IL-5, IL-13. Also, total cell number of ALN and dorsal skin tissue, absolute cell number of CD3e+&CD19+, CD4+&CD8+, CD3+/CCR3+, CCR3+, CD3+/CD69+, CD3+/CXCR5+ in ALN, PBMCs, absolute cell number of CCR3+, CD3+/CD69+, CD11b+/Gr-1+ in dorsal skin tissue, Eotaxin2 mRNA, CCR3 mRNA in dorsal skin tissue and gene expression of IL-5 mRNA, IL-13 mRNA in ALN are significantly decreased. Furthermore, thickness of epidermis, infiltrated inflammatory immune cell & mast cell in dermis, histologic infiltration of mast cell, the size of inflammatory lymphocytes cells & plasma cells in ALN and histologic infiltration of CD4+ & CCR3+ in ALN and dorsal skin tissue are significantly decreased. However, total cell number of DLN, absolute cell number of CD3+&CD19+, CD4+&CD8+, B220+/CD23+, CD3+/CD69+ in DLN and CD4+CD25+foxp3+Treg cell, foxp3 mRNA in dersal skin tissue are increased significantly. Conclusions : Concurrent administration of KKSDU and AJ on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse was very effective to the atopic detmatitis treatment.

• 생물환경조절학회지
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• 제16권1호
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• pp.62-66
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• 2007

시금치 수경재배에 적합한 육묘배지 선발과 육묘 플러그 트레이 크기, 육묘 플러그 트레이 셀(cell)당 종자수 조절로 안정 공정 육묘생산을 위한 실험을 수행하였다. 수경재배 육묘배지에 따른 입모율과 유묘의 생육특성을 조사한 결과 입모율은 보습성이 좋은 입상암면 >입상암면+펄라이트 혼용배지>코코피트>펄라이트>우레탄 스펀지 순으로 나타나 우레탄 스폰지의 입모율이 가장 낮았다. 육묘 플러그 트레이 셀당 종자수에 따른 엽 면적은 종자수가 많을수록 적어지고, 셀당 생체중은 2립(12.5g)에 비해 4립(33.9g)이 무거웠다. 따라서 셀당 종자수가 적으면 식물체 1개 체중은 증가되지만 전체 셀당 생체중은 감소되는 경향을 나타내었다. 수량은 셀당 2립 파종 $10,200kg{\cdot}ha^{-1}$ 비해 4립 파종 $14,910kg{\cdot}ha^{-1}$으로 46% 증가되었다.

• 한국통신학회논문지
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• 제38A권8호
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• pp.724-732
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• 2013

본 논문은 다중 사용자 (multiuser) 다중 안테나 (MIMO, multiple-input and multiple-output) 시스템을 기반으로 거대 안테나 시스템 (massive MIMO system)에 대한 성능 분석을 진행한다. 하향 링크 프레임 구조를 고려한 평균 셀 용량을 도출하고, 해당 평균 셀 용량을 기지국 안테나 수 및 사용자 수에 대하여 분석한다. 평균 셀 용량은 기지국 안테나 수 및 사용자 수에 대해 오목 함수 (concave function)이며 이러한 특징을 통해 최적의 기지국 안테나 수 및 사용자 수를 도출한다. 실험 결과를 통해 수식적으로 도출한 최적 안테나 수 및 사용자 수는 실험을 통한 최적 값과 일치함을 확인하였으며 도출한 최적 값을 통해 최대 값의 평균 셀 용량을 얻을 수 있음을 확인할 수 있다.

• Journal of Plant Biology
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• 제28권2호
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• pp.141-148
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• 1985

당근(Daucus carota L.) 세포 배양시 무성배 발생에 미치는 세포의 크기, 세포의 밀도, conditioned medium및 배지의 pH효과를 구명하기 위하여 실험한 결과는 다음과 같다. 세포배양시 구상형 배의 발생은 배양 4일째 최대에 달했으며 그 이후부터는 심장형 및 어뢰형 배로 발달되었다. 세포의 밀도가 높을수록 배 발생수가 증가하였으며 MS배지보다는 conditioned medium에서 양호하였고 배의 발달도 촉진되었다. 세포의 크기가 작을수록 생체중 및 배의 형성수가 증가하였으며 9o$\mu\textrm{m}$이상의 세포에서는 현저히 억제되었다. 배지의 pH가 4.0 혹은 7.0일때는 세포의 건물중이 현저히 감소하였고 배의 발생도 억제되었다. 그러나 pH 6에서 건물중이 최대에 달하였고 배의 발생도 양호하였다.

• Clinical and Experimental Reproductive Medicine
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• 제44권4호
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• pp.193-200
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• 2017

Objective: This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. Methods: First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed. Results: The hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p< 0.05). In the control-8 group ($22.1{\pm}4.6$), the cell number of the inner cell mass was higher than in the LAH groups (p< 0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group ($92.8{\pm}8.9$) than in the others (p< 0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group ($19.5{\pm}5.1$, p< 0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group ($73.2{\pm}12.1$) than in the other groups, but without statistical significance. Conclusion: When LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.

• Bulletin of the Korean Chemical Society
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• 제18권3호
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• pp.291-295
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• 1997

The adsorption of methane in K+ ion exchanged zeolite L has been studied using grand canonical ensemble Monte Carlo simulation. Average number of molecules per unit cell, number density of molecules in zeolite, distribution of molecules per unit cell, average potential per sorbate molecule, and isosteric heats of adsorption were calculated, and these results were compared with experimental results. The simulation results agreed fairly well with experimental ones. All methane molecules were located in the main channel, and the average potential of sorbate molecule was almost constant regardless of average number of molecules per unit cell and the amounts sorbed in zeolite.

• 한국수소및신에너지학회논문집
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• 제27권1호
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• pp.42-48
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• 2016

This study presents an experiment investigation on natural convection heat transfer of air-cooling Proton exchange membrane fuel cells (PEMFCs) in a enclosure system for unmanned aerial vehicles (UAVs). Considered are replacing fuel cell stack with Aluminum block for heat generating inside a enclosure chamber. The volume ratio of fuel cell stack and chamber for simulation to the actual size of aerial vehicle is 1 to 15. The parameters considered for experimental study are the environmental temperature range from $25^{\circ}C$ to $-60^{\circ}C$ and the block heat input of 10 W, 20 W and 30 W. Effect of the thermal conductivity of the block and power level on heat transfer in the chamber are investigated. Experimental results illustrate the temperature rise at various locations inside the chamber as dependent upon heat input of fuel cell stack and environmental temperature. From the results, dimensionless correlation in natural convection was proposed with Nusselt number and Rayleigh number for designing air-cooling PEMFC powered high altitude long endurance (HALE) UAV.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2014년도 제49회 KOSCO SYMPOSIUM 초록집
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• pp.175-178
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• 2014

The flame cell dynamics in 2-D opposed nonpremixed tubular configuration was investigated using high-fidelity numerical simulations. The diffusive-thermal instability occurs as the $Damk{\ddot{o}}hler$ number, Da, approaches the 1-D extinction limit of the tubular flames and several flame cells are generated depending on Da, and flame radius. In general, the number of flame cells are found close to the largest wave number from the linear stability analysis. It was also found from the displacement speed analysis that during the local flame extinction and cell formation, negative edge flame speed is observed due to small gain from reaction compared to large loss from diffusion.