• Journal of Applied Biological Chemistry
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• 제43권3호
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• pp.176-179
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• 2000

Nonpolar taxoides extracted from a large-scale cell culture of Taxus chinensis were isolated through the normal and reverse phase column chromatographies, and their compounds were identified via NMR spectroscopy. The complete separation method was systematically established and described. In dichloromethane, dissolved paclitaxel and other taxoids with hexane were precipitated during the purification of paclitaxel from the plant cell culture of T. chinensis through a large-scale process while the relatively nonpolar taxane derivatives remained dissolved in the hexane phase. 13-Deoxy baccatin III (I), baccatin VI (II), taxchinin I (III), $2{\alpha}$, $5{\alpha}$, $10{\beta}$, $14{\beta}$-tetraacetoxy-4(20), 11-taxadiene(IV), 1-deoxy baccatinVI(V), and taxayuntin C (VI) were isolated through column chromatography and identified via NMR spectroscopy. Compounds I and IV were found to the major components, aside from paclitaxel, in the plant cell culture of T. chinensis. The concentrations of I and IV were compared with the that concentration of the paclitaxel in each of plant cell culture. The possible applications of compounds I, II, IV, and V were discussed.

• 한국미생물·생명공학회지
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• 제20권6호
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• pp.630-636
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• 1992

토양으로부터 19균주의 메탄올 자화성 효모를 분리하였으며, 이들 중 높은 균체농도와 aldehyde 생산을 보인 균주를 선별 및 동정하여 Hansenula nonfermentant KYP-1으로 명명하였다. Aldehyde 생산은 메탄올 자화성 효모의 균체를 생촉매로 하는 resting cell system에서 행하였으며, 조사된 aldehyde 가운데 acetaldehyde의 생산량이 가장 높았다. 최대의 aldehyde 생산은 40시간 배양한 균체를 생촉매로 이용하였을 때 얻어졌다.

• KSBB Journal
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• 제15권6호
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• pp.560-565
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• 2000

13-Deacetyl-taxchinin I, a taxoid having a rearranged 11(15\longrightarrow1)-abeo-taxane skeleton, has been isolated and identified from plant cell cultures of Taxus chinensis. The compound has not previously been encountered in nature. Its structure was elucidated by 1-and 2D NMR techniques including H-H COSY, HMQC, and HMBC experiments. This taxoid also provides information for better understanding of structure-activity relationships and biosynthesis, as well as improving the quality control of paclitaxel production.

• 한국환경보건학회지
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• 제23권4호
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• pp.33-38
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• 1997

Four bacterial strains able to degrade dichlorobenzene as the sole source of carbon and energy were isolated from soil by selective enrichment culture, and among them, one isolation was the best in the cell growth and identified as Pseudomonas sp. DCB3 by its morphology and physiological properties. Cell growth dramatically increased in a minimal medium containing 500ppm of dichlorobenzene was not detected any more at 72 hours after cultivation. The optimal temperature and initial pH for the growth of the isolated strain were 30$\circ$C and 7.0, respectively. Cell growth was increased by supplementing $(NH_2)_2CO$ and 50 ppm yeast extract as additional nutrients. Therefore, it was suggested that Pseudomonas sp. DCB3 could be effectively used for the biological treatment of wastewater containing dichlorobenzene.

• 생약학회지
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• 제28권4호
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• pp.225-232
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• 1997

300 extracts derived from 100 plants were tested for their potential to induce HL-60 cell differentiation using NBT assay and NSE/SE staining methods. Morphological changes from suspended to adherent state of the cells were also observed by microscopic examination. In result, 55 extracts induced cell differentiation into monocyte/macrophage lineage in the NBT and the NSE assay.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2002년도 The 11th Korea Genome Conference
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• pp.50.1-50.1
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• 2002

• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.2006-2013
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• 2019

The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

• 한국수정란이식학회지
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• 제26권2호
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• pp.111-115
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• 2011

In general, zona pellucida (ZP) of the blastocyst has to be removed first, then either isolated the inner cell mass (ICM) or ZP-removed whole blastocyst, which is then cultured on the feeder layer to induce ICM outgrowth for the generation of embryonic stem cells (ESC). However, it is unclear whether ICM isolation before seeding on feeder layer is beneficial or not because the interaction between ICM and trophoblasts may affect cellular growth and/or pluripotency during the culture on the feeder. In the present study, two ZP removal methods (mechanically by splitting with a 28-gauge needle versus chemically by the treatment of acid-Tyrode's solution) and two ICM isolation methods (ZP-free whole blastocyst seeding versus mechanical isolation of ICM) were evaluated for the efficient isolation and culture of putative parthenogenetic bovine ESC. The number of maintained outgrown colonies was counted in each experimental group. As the result, mechanical removal of ZP with a needle and followed by whole ZP-free blastocyst seeding on feeder cells tended to attach more on the feeder layer and resulted in more outgrown colonies with its simple and less time-costing benefits. Currently we are generating ESC lines in HanWoo cattle by using this method for initial outgrowth of the parthenogenetic bovine blastocysts.

• 한국전자파학회논문지
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• 제29권10호
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• pp.739-744
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• 2018

본 논문에서는 비발디 안테나 소자 간 격리도 향상을 위해, 메타구조 기반 흡수체의 한 종류인 double split ring resonator(DSRR)를 제안하였다. Unitcell 모의실험을 통하여 흡수체로 동작하는 DSRR을 설계하였으며, DSRR을 통한 격리도 향상을 확인하기 위하여 $1{\times}2$ 비발디 안테나에 적용하였다. 제안된 DSRR의 unitcell 크기는 $5mm{\times}5mm{\times}1.52mm$이며, 총 6개의 unitcell이 사용되었다. 제안된 DSRR의 성능 검증을 위하여 DSRR 적용 유무에 따른 $1{\times}2$ 비발디 안테나를 제작 및 측정하였으며, 측정 결과 DSRR이 적용된 비발디 안테나는 DSRR이 적용되지 않은 비발디 안테나에 비해 20 dB의 격리도 향상을 보였다.

• 한국작물학회지
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• 제27권2호
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• pp.147-154
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• 1982

식물원형질체를 이용하여 체세포잡종을 육성하기 기초자료를 얻고자 식물의 엽육부위, Callus에서 효과적인 원형질체의 분리, 적합한 배양조건 및 세포융합에 관한 조건을 조사한 결과를 요약하면 다음과 같다. 1. 배양 융합조건에 적합한 식물원형질체의 분리 방법은 여러 종류의 효소농도에 따라 다르지만 담배 및 완두엽육 원형질체분리에는 Macerozyme 0.5%, Cellulase 2.0%, KDS 0.5%, Mannitol 0.7M의 효소용액에 처리시 활성 있는 원형질체를 얻을 수 있었다. 2. 배양중인 담배 및 대두의 Callus로부터 원형질체분리는 배양기간에 따라 세포활성도가 떨어지거나 쉽게 파괴되는 현상을 보여 양호한 원형질체를 얻을 수가 없었다. 3. 원형질체의 배양은 배양배지에 Plating할 경우 세포농도에 따라 차이가 있었으며 1.0$\times$$10^4$/ml이상이어야 분열이 가능하였고 배양조건은 150Lux 12시간 광암처리후가 세포분열 및 증식이 가장 왕성하였다. 4. 세포융합은 PEG농도가 중요하며 PEG 1500 0.33M에서 CaCl_2농도가 높을수록 융합율이 증가되는 경향이었다.