• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.117-117
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• 2002

The oocytes from most of animal species accumulate genetic information and other necessary materials during oogenesis for the later use in the early development. Over the years oocyte maturation has been studied extensively both in vitro and in vivo. Particularly, maturation of follicular oocyte in vitro becomes one of the important tools for the studies of basic cell biology, the in vitro technology of animal production, and in particular, the somatic cell cloning by nuclear transfer. We examined meiotic maturation and cumulus expansion in the presence of translation or transcription inhibitors for varying periods of in viかo maturation (IVM) of pig oocyte. In Experiment 1, the results revealed that translation and transcription inhibitors inhibited cumulus expansion and meiotic maturation during 35h of IVM. However, 50 to 60% of the oocytes underwent nuclear maturation without cumulus expansion during 75h of IVM. The rest of the oocytes were arrested at metaphase I (40-50%) in the presence of the inhibitors. In Experiment II, the OCCs were exposed to the drugs only for 15h to examine translation and transcription inhibitors on cumulus expansion and meiotic maturation. Transcription inhibitors for 15h did not arrest meiotic maturation when the oocytes were cultured for subsequent, necessary period of IVM, whereas cumulus expansion was completely inhibited, suggesting that initial 15h is critical transcription activity far cumulus expansion. Translation inhibitors for 15h exposure did not alter cumulus expansion and meiotic maturation during subsequent culture in the absence of the drugs. In Experiment III, the OCCs were exposed to the drugs only for later 30h to examine the influence of transcription and translation inhibitors on oocyte maturation. Interestingly, all meiotic maturation underwent normally with full expansion of cumulus. Similar results were obtained from Experiment IV where 5h of exposure from 15 to 20h of IVM culture to the drugs was performed and subsequently cultured for same period in fresh medium. Taken there results together, both transcription and translation are necessary for nuclear maturation and cumulus expansion, and first 15h IVM for cumulus expansion is critical. The arrested oocytes by the drugs were still capable of undergoing nuclear maturation, although cumulus expansion was affected.

• 한국수정란이식학회지
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• 제33권4호
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• pp.287-296
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• 2018

Ganglioside GM3 is known as an inhibition factor of cell differentiation and proliferation via inhibition of epidermal growth factor receptor (EGFR) phosphorylation. Our previous study showed that the exogenous ganglioside GM3 reduced the meiotic maturation of porcine oocytes and induced apoptosis at 44 h of in vitro maturation (IVM). However, the role of ganglioside GM3 in the relationship between EGFR signaling and apoptosis during porcine oocyte maturation has not yet been studied. First, porcine cumulus-oocyte complexes (COCs) were cultured in the NCSU-23 medium with exogenous ganglioside GM3 according to maturation periods (non-treated, only IVM I: 0 - 22 h, only IVM II: 22 - 44 h and IVM I & II: 0 - 44 h). We confirmed that the proportion of germinal vesicle breakdown (GVBD) increased significantly in the IVM I treated group than in the control group. We also confirmed that the meiotic maturation until M II stage and polar body formation decreased significantly in the only IVM I treated group. Cumulus cell expansion and mRNA levels of the expansion-related factors (HAS2, TNFAIP6 and PTX3) decreased significantly in the IVM I treated group than in the control group. Protein levels of EGFR, p-EGFR, ERK1/2, and p-ERK1/2 decreased significantly in the GM3-treated groups, during the IVM I period. In addition, cellular apoptosis, determined using TUNEL assay, and protein levels of Cleaved caspase 3, were increased significantly in the GM3-treated COCs during the IVM I period. Based on these results, ganglioside GM3 exposure of porcine COCs during the IVM I period reduced meiotic maturation and cumulus cell expansion via inhibition of EGFR activity in pigs.

• 대한치과교정학회지
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• 제50권1호
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• pp.42-51
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• 2020

Objective: The objective of this study was to evaluate the effects of cetirizine, a histamine 1 receptor antagonist, on bone remodeling after calvarial suture expansion. Methods: Sixty male Sprague-Dawley rats were divided into 4 groups; the phosphate-buffered saline (PBS)-injected no expansion group, cetirizine-injected no expansion group, PBS-injected expansion group, and cetirizine-injected expansion group, and were observed at 7, 14, and 28 days. Five rats per group were examined at each observation day. Daily injections of cetirizine or PBS were administered to the relevant groups starting 2 weeks prior to expander insertion. A rapid expander was inserted in the calvarial bone to deliver 100 cN of force to the parietal suture. The specimens were prepared for hematoxylin and eosin and tartrate-resistant acid phosphatase (TRAP) staining. Suture opening and bone regeneration were evaluated using microcomputed tomography and bone histomorphometric analysis. Serum blood levels of osteocalcin and carboxy-terminal collagen crosslinks (CTX) were also evaluated. Results: TRAP-positive cell counts and CTX levels decreased while osteocalcin levels increased in the cetirizine-injected expansion group at observation day 28. In the expansion groups, the mineralized area gradually increased throughout the observation period. At day 28, the cetirizine-injected expansion group showed greater bone volume density, greater mineralized area, and narrower average suture width than did the PBS-injected expansion group. Conclusions: Cetirizine injection facilitated bone formation after suture expansion, mostly by suppressing osteoclastic activity. Histamine 1 receptor antagonists may aid in bone formation after calvarial suture expansion in the rat model.

• Clinical and Experimental Reproductive Medicine
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• 제13권2호
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• pp.201-206
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• 1986

These experiments were conducted to know whether RNA syntheis is involved in the cumulus expansion and oocyte maturation of mouse cumulus-oocyte complexes in vitro. Mouse cumulus-oocyte complexes(COC's) were cultured in the presence of cordycepin, an inhibitior of RNA synthesis and its effect on the cumulus expansion and oocyte maturation were examined. The results were as follows. 1. Continuous presence of cordycepin in the medium(200${\mu}g/ml$) inhibited the HCG-induced cumulus cell expansion of mouse complexes. This inhibition was reversible. 2. When the COC'S were preincubated with different concentration of cordycepin plus HCG for 3 hours and then transferred to the plain medium, the HCG induced cumulus expansion was suppressed at $100{\mu}g/ml$ of cordycepin. 3. When the COC'S were cultured with cordycepin after HCG stimulation(3hrs), the cumulus expansion were not suppressed by cordycepin. 4. Oocyte meiotic maturation did not appear to be affected by cordycepin. The data presented here imply that RNA synthesis is involved in the cumulus expansion process and that mouse oocytes are more resistant to RNA synthesis inhibitor than cumulus cells.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.95-95
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• 2003

• 한국정보통신학회논문지
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• 제17권12호
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• pp.2806-2811
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• 2013

최근에 폭발적으로 증가하고 있는 모바일 데이터 트래픽을 효과적으로 수용하기 위하여 다중 안테나와 소형 셀과 같은 신기술들이 차세대 이동통신 시스템의 핵심 기술로 제안되었다. 특히, 기존의 매크로 셀과 소형 셀을 동시에 활용하여 공간 재사용율을 높일 수 있는 이종 이동통신 네트워크에 대한 관심이 높아지고 있다. 그러나, 이종 네트워크에서는 단말들이 소형 기지국 대비 상대적으로 송신 출력이 높은 매크로 기지국을 통해서 서비스 받을 가능성이 높기 때문에 소형 기지국의 활용도가 상대적으로 낮아지는 문제점이 있으며, 이러한 문제점은 상향 링크에서 두드러지게 나타난다. 3GPP에서는 이러한 문제점을 해결하기 위하여 셀 확장 편향치 개념을 제안하고 이에 대한 표준화를 진행하고 있다. 본 논문에서는 셀 확장 편향치를 적용한 네트워크 시뮬레이터를 이용하여 이종 이동 통신 네트워크의 하향 링크 성능을 평균 셀 전송 효율 측면에서 분석한다.

• E2M - 전기 전자와 첨단 소재
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• 제10권8호
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• pp.843-851
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• 1997

• 인터넷정보학회논문지
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• 제24권5호
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• pp.1-7
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• 2023

본 논문은 매크로 기지국 (Macro base station, MBS) 커버리지 내 스몰셀 기지국 (Small-cell base station, SBS)이 중첩된 5G 이기종 네트워크 (Heterogeneous network, HetNet)환경에서 머신 타입 통신 장치 (Machine-Type Communications Devices, MTCD)들에게 미치는 간섭을 해결하고 성능향상을 위한 스몰셀 자원 분리 할당 방법을 제안한다. 5G HetNet에서는 다양한 타입의 MTCD들이 데이터 트래픽을 생성하므로 기지국에 대한 부하가 심해진다. 따라서 이 부하를 해결하기 위해 SBS의 수신세기에 bias값을 더해 조건에 만족하는 MTCD들을 SBS에 연결하는 셀 범위 확장 (Cell range expansion, CRE)방법이 적용된다. CRE를 통해 SBS에 연결되는 MTCD들의 수가 증가하여 MBS의 부하는 줄어들지만 간섭의 영향을 받아 성능이 저하되므로 이를 해결하는 방법이 필요하다. 제안하는 스몰셀 자원 분리 할당 방법은 CRE로 SBS 내 새롭게 추가된 MTCD의 간섭 완화를 위해 기존 MBS로부터의 간섭이 적은 자원을 할당하고 기존 MTCD들의 성능에 따라 자원을 분리하여 할당하므로 전체 MTCD들의 성능을 향상시킨다. 시뮬레이션 결과를 통해 제안하는 스몰셀 자원 분리 할당 방법은 기존의 자원 할당 방법보다 MBS와 SBS에 연결된 MTCD들의 시스템 용량에서 21%와 126%의 성능향상을 보인다.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제48권8호
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• pp.570-576
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• 1999

$LaCrO_3$-dispersed Cr alloys for metallic interconnector of solid oxide fuel cell have been studied as function of $LaCrO_3$ content in the range of 5 to 25 vol.% in order to examine the electric conductivity, the oxidation property and the thermal expansion behavior of these alloys. The $LaCrO_3$-dispersed Cr alloys showed high electrical conductivities of $3~5\times10^4$ S/cm at room temperature, and as the $LaCrO_3$content increased the conductivity decreased slightly. During the cyclic oxidation test at $1100^{\circ}C$, the weight change of the Cr alloys decreased with increasing number of oxidation cycle except first cycle, which is attributed to the vaporization of the oxide scale. More addition of the $LaCrO_3$ content reduced also the weight change of the Cr alloys. These mean that the oxide scale formed at the surface of the Cr alloy becomes stable with increasing number of oxidation cycle and$LaCrO_3$ content. The measured thermal expansion of the Cr alloy was well fitted to that of 8 mol% $Y_2O_3$-stabilized $ZrO_2$ electrolyte. These results demonstrate that $LaCrO_3$-dispersed Cr alloy is a useful material for metallic interconnector of solid oxide fuel cell.

• KSBB Journal
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• 제19권5호
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• pp.335-340
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• 2004

Mesangial expansion caused by cell proliferation and glomerular extracellular matrix accumulation is one of the earliest renal abnormalties observed at the onset of hyperglycemia in diabetes mellitus. Transcription factor Sp1 is implicated in the transcriptional regulation of a wide range of genes participating in cell proliferation, and is assumed to play an essential role in mesangial expansion, transforming growth factor (TGF)-$\beta$1, plasminogen activator inhibitor (PAI)-1. We have generated a phosphorothioated double-stranded Sp1-decoy oligodeoxynucleotide that effectively blocks Sp1 binding to the promoter region for transcriptional regulation of TGF-$\beta$1 and PAI-1. The Sp1 decoy oligodeoxynucleotide suppressed transcription of these cytokines and proliferation of primary rat mesangial cells in response to serum stimulation. These results suggest that the Sp1 decoy oligodeoxynucleotide could bea powerful tool in preventing the pathogenesis of renal hypertrophy.