• KSBB Journal
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• 제15권4호
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• pp.405-407
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• 2000

A novel recovery method was developed to obtain the plant cell and its debris from Taxus chinensis cell cultures By pH control of plant cell cultures plant cell and debris was precipitated. The best pH is between 1.8 and 2.2 to obtain the precipitate of the plant cell and debris. Also paclitaxel is stable in this acidic conditions. This method is very simple an efficient to recover the plant cell and debris from plant cell cultures.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1990년도 제12회 학술강연회초록집
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• pp.58-62
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• 1990

The size and concentration of wear debris in lubricating oil often reveal the operating condition of the rotating machinery. To evaluate the possible application of light attenuation measurement for the estimation of wear debris concentration in the lubricating oil, the light transmittance through the lubricating oil cell contaminated with various concentrations of diatomite particles was measured, the attenuation coefficient was estimated from the transmittance measurement and the coefficients were compared with those obtained from the scattering theory. The comparision showed good agreements between them. It is also noted that the experimentally determined attenuation coefficient showed almost linear relation with particle weight concentrations for the concentrations within the range of 2000 ppm. For the case of 0 ppm weight concentration of diatomite particles in the lubricating oil cell, the thickness of the cell required to give $100 \mu W$ light attenuation is 7.75 mm. This result indicates that the light attenuation method will be one of the possible candidates of machine failure diagnostic sensors for the estimation of wear debris concentration in the lubricating oil.

• Tribology and Lubricants
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• 제6권2호
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• pp.94-98
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• 1990

The size and concentration of wear debris in lubricating oil often reveal the operating condition of the rotating machinery. To evaluate the possible application of light attenuation measurement for the estimation of wear debris concentration in the lubricating oil, the light transmittance through the lubricating oil cell contaminated with various concentrations of diatomire particles was measured, the attenuation coefficient was estimated from the transmittance measurement and the coefficients were compared with those obtained from the scattering theory. The comparision showed good agreements between them. It is also noted that the experimentally determined attenuation coefficient showed almost linear relation with particle weight coucentrations for the concentrations within the range of 2000 ppm. For the case of 0 ppm weight concentration of diatomire particles in the lubricating oil cell, the thickness of the cell required to give $100 \muW$ light attenuation is 7.75 mm. This result indicates that the light attenuation method will be one of the possible candidates of machine failure diagnostic sensors for the estimation of wear debris concentration in the lubricating oil.

• 한국막학회:학술대회논문집
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• 한국막학회 1994년도 추계 총회 및 학술발표회
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• pp.1-6
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• 1994

Many useful biomaterials like enzymes are contained in yeast cells. However, the release of these intracellular biomateriais from the cells is required to recover them with hot water, solvent or various cell breakage methods of mechanical or non mechanical ones. The cell lysis or breakage of yeast is usually made by solvent like ethyl acetate and mechanical disintrgration with high pressure homogenizer or agitating beads mill. The separation of cell debris (i.e. solid liquid separation) is done by centrifuge or membrane depending on the recovery conditions. The features of both separation methods are shown in Tables 1 and 2. As it is often difficult to obtain a clear supernatant by centrifuge from the suspension containing cell debris, the membrane separation is also often used to gel a clear supernatant. In this report we introduce the several applications of membrane separation to separate the cell debris of yeast disintegrated chemically or mechanically and to recover the intracellular biomaterials.

• Journal of Microbiology and Biotechnology
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• 제6권4호
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• pp.225-231
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• 1996

The production of recombinant proteins in Escherichia coli often leads to the formation of an intracellular inclusion body. Key process steps that can determine the economics of large-scale protein production from inclusion bodies are fermentation, inclusion body recovery, and protein refolding. Compared with protein refolding and fermentation, inclusion body recovery has received scant research attention. Nevertheless, it can control the final product yield and hence process cost for some products. Optimal separation of inclusion bodies and cell debris can also aid subsequent operations by removing contaminant particulates that foul chromatographic resins and contain antigenic pyrogens. In this review, the properties of inclusion bodies and cellular debris are therefore examined. Attempts to optimise the centrifugal separation of inclusion bodies and debris are also discussed.

• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.189-193
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• 1996

Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently willi the cell debris removal process using SSPP.

• ALGAE
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• 제31권4호
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• pp.289-301
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• 2016

Floating debris provides substrates for dispersal of organisms by ocean currents, including algae that thrive on plastics. The 2011 earthquake and tsunami in Tohuku, Japan resulted in large amounts of debris carried by the North Pacific Current to North America from 2012 to 2016. In 2015-2016, the plastics in the debris bore a complex biota including pink algal crusts. One sample (JAW4874) was isolated into culture and a three-gene phylogeny (psbA, rbcL, and SSU) indicated it was an unknown member of the red algal class Stylonematophyceae. It is a small pulvinate crust of radiating, branched, uniseriate filaments with cells containing a single centrally suspended nucleus and a single purple to pink, multi-lobed, parietal plastid lacking a pyrenoid. Cells can be released as spores that attach and germinate to form straight filaments by transverse apical cell divisions, and subsequent longitudinal and oblique intercalary divisions produce masses of lateral branches. This alga is named Tsunamia transpacifica gen. nov. et sp. nov. Sequencing of additional samples of red algal crusts on plastics revealed another undescribed Stylonematophycean species, suggesting that these algae may be frequent on drift oceanic plastics.

• 한국정보기술학회논문지
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• 제17권6호
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• pp.85-94
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• 2019

기두부와 단 분리 시 조각은 서로 다른 미세 운동을 하므로 스펙트로그램 상에서 미세 도플러 주파수의 형태가 서로 다르게 나타나며 이를 통해 구분이 가능하다. 본 논문에서는 합성곱 신경망(CNN : Convolutional Neural Networks)을 이용하여 기두부와 단 분리 시 조각을 구분하였다. 합성곱 신경망의 입력영상으로는 미세도플러 스펙트로그램을 사용하였다. 또한 기두부와 단 분리 시 조각의 구분성능을 향상시키기 위해 미세 도플러 스펙트로그램에 CA-CFAR(Cell Averaging-Constant False Alarm Rate)를 적용하여 전처리 과정을 수행하였다. 실험 결과, 전처리 과정을 수행하여 획득한 미세 도플러 스펙트로그램을 입력 영상으로 사용하였을 경우, 전처리 과정을 수행하지 않은 미세 도플러 스펙트로그램보다 모든 SNR환경에서 구분 성능이 향상되었다.

• 한국지반신소재학회논문집
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• 제17권4호
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• pp.267-275
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• 2018

사방댐 배면의 준설퇴적물에 대한 준설시기를 객관적, 정량적, 과학적으로 결정할 수 있도록 준설퇴적물 측정장치를 개발하고 실제 현장에 시범 구축하였다. 사방댐 배면의 준설퇴적물 하중을 측정하기 위하여 준설퇴적물 관리시스템을 설계 및 개발하였다. 본 관리시스템은 Data Acquisition System (DAS), Solar System 및 준설퇴적물 하중변화 측정유닛으로 구성되어 있다. 또한 사방댐 배면의 준설퇴적물 하중, 수위 및 강우량을 실시간으로 측정하고 무선통신을 통하여 자료를 전송할 수 있다. 준설퇴적물 하중변화 측정유닛은 강우를 측정하기 위한 강우계, 사방댐 배면의 준설퇴적물 하중을 측정하기 위한 하중측정계, 사방댐 배면 수위를 측정하기 위한 수위계로 구성되어 있다. 사방댐 배면의 준설퇴적물 관리기준은 준설퇴적물의 하중을 기준으로 제안하였다. 사방댐 배면에 퇴적될 수 있는 최대 준설퇴적물의 양을 산정하고, 최대 준설퇴적물의 50%, 70% 및 90%가 쌓인 경우 관심, 주의 및 경보의 단계로 구분하여 관리기준을 마련하였다. 사방댐 배면의 준설퇴적물 관리시스템을 활용하여 현재 준설퇴적물의 하중을 실시간으로 측정할 수 있으며, 이를 토대로 사방댐의 상태와 준설퇴적물의 준설시기를 결정할 수 있을 것으로 판단된다.

• 한국산업융합학회 논문집
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• 제23권5호
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• pp.809-815
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• 2020

A number of investigations and studies have been conducted in various fields regarding the sediment disasters of Mt. Woomyeon that occurred in July 2011. We collected and compared the topographic information of the general points where debris flows did not occur and the collapse points where the debris flow occurred in order to find out the characteristics of the collapse points in Woomyeon mountain. The collected topographic information is altitude, curvature, slope, aspect and TPI(topographic position index). As a result of comparison, there were relatively many collapse points at an altitude of 210m to 250m, and at a slope of 30° to 40°. In addition, the risk of collapse was low in a cell where the curvature was close to 0, and the risk was higher in concave terrain than in convex terrain. In the case of TPI, there was no statistical difference between the general points and the collapse points when the analysis radius was larger than 200m, and there was a correlation with the curvature when the analysis radius was smaller than 50m. In the case of debris flows that are affected by artificial structures or facilities, there is a possibility of disturbing the topographic analysis results. Therefore, if a research on debris flow is conducted on a mountain area that is heavily exposed to human activities, such as Woomyeon mountain, diversified factors must be considered to account for this impact.