• Title/Summary/Keyword: Cell biology

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Efficient Derivation of New Human Embryonic Stem Cell Lines

  • Kim, Sun Jong;Lee, Jeoung Eun;Park, Jong Hyuk;Lee, Jung Bok;Kim, Jin Mee;Yoon, Byung Sun;Song, Ji Min;Roh, Sung Il;Kim, Chul Geun;Yoon, Hyun Soo
    • Molecules and Cells
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    • v.19 no.1
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    • pp.46-53
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    • 2005

  • Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.

  • KSCI

The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro

  • Moon, Byoung-San;Ammothumkandy, Aswathy;Zhang, Naibo;Peng, Lei;Ibrayeva, Albina;Bay, Maxwell;Pratap, Athira;Park, Hong Ju;Bonaguidi, Michael Anthony;Lu, Wange
    • Clinical and Experimental Otorhinolaryngology
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    • v.11 no.4
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    • pp.224-232
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    • 2018

  • Objectives. Spiral ganglion neurons (SGNs) include potential endogenous progenitor populations for the regeneration of the peripheral auditory system. However, whether these populations are present in adult mice is largely unknown. We examined the presence and characteristics of SGN-neural stem cells (NSCs) in mice as a function of age. Methods. The expression of Nestin and Ki67 was examined in sequentially dissected cochlear modiolar tissues from mice of different ages (from postnatal day to 24 weeks) and the sphere-forming populations from the SGNs were isolated and differentiated into different cell types. Results. There were significant decreases in Nestin and Ki67 double-positive mitotic progenitor cells in vivo with increasing mouse age. The SGNs formed spheres exhibiting self-renewing activity and multipotent capacity, which were seen in NSCs and were capable of differentiating into neuron and glial cell types. The SGN spheres derived from mice at an early age (postnatal day or 2 weeks) contained more mitotic stem cells than those from mice at a late age. Conclusion. Our findings showed the presence of self-renewing and proliferative subtypes of SGN-NSCs which might serve as a promising source for the regeneration of auditory neurons even in adult mice.

  • https://doi.org/10.21053/ceo.2018.00878 인용 KSCI