• 제목/요약/키워드: Cell Temperatures

검색결과 690건 처리시간 0.046초

Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37℃ and 39℃ for cultured meat

  • Sehyuk Oh;Sanghun Park;Yunhwan Park;Yun-a Kim;Gyutae Park;Xiangshun Cui;Kwansuk Kim;Seontea Joo;Sunjin Hur;Gapdon Kim;Jungseok Choi
    • Journal of Animal Science and Technology
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    • 제65권3호
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    • pp.664-678
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    • 2023
  • To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37℃ and 39℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37℃ proliferated better than cultured at 39℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Hanwoo myosatellite cells cultured at 39℃ had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37℃. C2C12 cells cultured at 39℃ showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37℃. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37℃ and differentiating at 39℃ are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.

개선된 태양전지 모델링 해석 (Analysis of improved solar cell modeling)

  • 김선자;정병환;박종찬;최규하
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 2004년도 전력전자학술대회 논문집(1)
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    • pp.113-116
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    • 2004
  • Output power of a photovoltaic system changes continuously as it strongly depends on the weather condition(isolation and temperature). Therefore, it is necessary the theoretical model realizes the electrical output characteristics of solar cell. Of several theoretical models for real solar cell, both parametric model and interpolation model are used widely. In this paper, we have propose a improved model of solar cell using its output characteristics that can be extended to calculate the rear solar cell characteristics at various temperatures and insolation. And more, the theoretical research of several models of solar cell using simulation analysis.

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자연급기 무가습 연료전지의 Dry-out조건 (Dry-out conditions of free-breathing PEMFC with dry $H_2$ gas)

  • 박상균
    • Journal of Advanced Marine Engineering and Technology
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    • 제30권2호
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    • pp.225-230
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    • 2006
  • This study investigates drying condition when a small fan is added to a operating the free-breathing proton exchange membrane fuel cells (PEMFCs) with dry $H_2$ and Air. Polarization tests were conducted on PEMFCs at cell temperatures between 30 and $50^{\circ}C$ under dry operation. In the results, the cell performance strongly depended on the cell temperature and the cathode gas stoichiometric flow rates. The cell performance increases as cell temperature decreases from 50 to $30^{\circ}C$. In the domain where the stoichiometry of air is quite large, reduction of the concentration overpotential compensated the increased internal resistance due to drying. The maximum performance was obtained at the small air flow rate beyond which flooding occurs. This indicates that the fan should be operated in the stoichiometry domain with a well designed cell structure to avoid flooding.

화학적으로 증착된 CdS 박막의 반응온도에 따른 물성 (Effect of Reaction Temperature on Properties of CdS Thin Films Prepared by Chemical Bath Deposition)

  • 송우창
    • 한국표면공학회지
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    • 제38권3호
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    • pp.112-117
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    • 2005
  • In this paper, CdS thin films, which were widely used as a window layer of the CdS/CdTe and the $CdS/CuInSe_2$heterojunction solar cell, were grown by chemical bath deposition, and the structural, optical and electrical properties of the films on reaction temperatures were investigated. Cadmium acetate and thiourea were used as cadmium and sulfur source, respectively. And Ammonium acetate was used as the buffer solution. As the reaction temperatures were increased, the deposition rate of CdS fllms prepared by CBD was increased and the grain size was large due to increasing reaction rate in solution, also optical transmittance of the films in visible lights was increased on rising reaction temperatures.

계란의 저장·보관 조건에 따른 위생적 품질 변화 (Effects of Storage Time and Temperature on the Hygienic Quality of Shell Eggs)

  • 김종규;박정영;김중순
    • 한국환경보건학회지
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    • 제41권6호
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    • pp.438-448
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    • 2015
  • Objectives: This study was performed to examine the effects of storage time and temperature and their interaction on the hygienic quality parameters of shell eggs. Methods: Eggs from 40-week-old Hy-Line Brown hens were sampled immediately after being laid and subjected to storage periods of four weeks at a refrigerated temperature ($4-5^{\circ}C$) or room temperature ($13.0-19.7^{\circ}C$). Interior/exterior qualities were examined every one week. Results: Weight loss was 2.4-3.1%. The initial specific gravity of the eggs was maintained until one week at both temperatures. Air cell size exceeded 4 mm when stored for one week at room temperature, and two weeks at refrigerated temperature. Albumen index and Haugh unit were significantly decreased at both temperatures after one week (p<0.001). Rapidly increased pH of the albumen with one week of storage was observed, regardless of temperature (p<0.001). Extension of the storage for up to four weeks at room temperature resulted in remarkable deterioration of eggshell quality and instrumental color as redness (a). Air cell size, albumen and yolk indices, Haugh unit, pH of albumen and yolk were found to be influenced by storage time and temperature (p<0.001). Interaction effects between storage time and temperature were also significant for air cell size, pH of albumen and yolk (p<0.001). Conclusion: The results suggest that air cell size and pH of albumen and yolk were important parameters influenced by storage time and temperature in shell eggs. Storage time was more influential for air cell size, and temperature for the pH of yolk. Both variables almost equally influenced the pH of albumen.

결정질 실리콘 태양전지 도핑 확산 공정에서 시간과 온도 변화에 의한 Drive-in 공정 연구 (Optimization of Drive-in Process with Various Times and Temperatures in Crystalline Silicon Solar Cell Fabrication)

  • 이희준;최성진;명재민;송희은;유권종
    • 한국태양에너지학회:학술대회논문집
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    • 한국태양에너지학회 2011년도 추계학술발표대회 논문집
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    • pp.51-55
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    • 2011
  • In this paper, the optimized doping condition of crystalline silicon solar cells with 156 ${\times}$ 156 mm2 area was studied. To optimize the drive-in condition in the doping process, the other conditions except drive-in temperature and time were fixed. After etching 7 ${\mu}m$ of the surface to form the pyramidal structure, the silicon nitride deposited by the PECVD had 75~80 nm thickness and 2 to 2.1 for a refractive index. The silver and aluminium electrodes for front and back sheet, respectively, were formed by screen-printing method, followed by firing in $400-425-450-550-850^{\circ}C$ five-zone temperature conditions to make the ohmic contact. Drive-in temperature was changed in range of $828^{\circ}C$ to $860^{\circ}C$ and time was from 3 min to 40 min. The sheet resistance of wafer was fixed to avoid its effect on solar cell. The solar cell fabricated with various conditions showed the similar conversion efficiency of 17.4%. This experimental result showed the drive-in temperatures and times little influence on solar cell characteristics.

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Preparation and Electrochemical Performance of Electrode Supported La0.75Sr0.25Ga0.8Mg0.16Fe0.04O3-δ Solid Oxide Fuel Cells

  • Yu, Ji-Haeng;Park, Sang-Woon;Woo, Sang-Kuk
    • 한국세라믹학회지
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    • 제48권5호
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    • pp.479-484
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    • 2011
  • In this paper, investigations of thick film $La_{0.75}Sr_{0.25}Ga_{0.8}Mg_{0.16}Fe_{0.04}O_{3-{\delta}}$ (LSGMF) cells fabricated via spin coating on either NiO-YSZ anode or $La_{0.7}Sr_{0.3}Ga_{0.6}Fe_{0.4}O_3$ (LSGF) cathode substrates are presented. A La-doped $CeO_2$ (LDC) layer is inserted between NiO-YSZ and LSGMF in order to prevent reactions from occurring during co-firing. For the LSGF cathode-supported cell, no interlayer was required because the components of the cathode are the same as those of LSGMF with the exception of Mg. An LSGMF electrolyte slurry was deposited homogeneously on the porous supports via spin coating. The current-voltage characteristics of the anode and cathode supported LSGMF cells at temperatures between $700^{\circ}C$ and $850^{\circ}C$ are described. The LSGF cathode supported cell demonstrates a theoretical OCV and a power density of ~420 mW $cm^2$ at $800^{\circ}C$, whereas the NiO-YSZ anode supported cell with the LDC interlayer demonstrates a maximum power density of ~350 mW $cm^2$ at $800^{\circ}C$, which decreased more rapidly than the cathode supported cell despite the presence of the LDC interlayer. Potential causes of the degradation at temperatures over $700^{\circ}C$ are also discussed.

Structural Transition of A-Type Zeolite: Molecular Dynamics Study

  • Song, Mee-Kyung;Chon, Hak-Ze
    • Bulletin of the Korean Chemical Society
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    • 제14권2호
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    • pp.255-258
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    • 1993
  • Molecular dynamics (MD) calculations were carried out in order to investigate the effect of MD cell size to predict the melting phenomena of A-type zeolite. We studied two model systems: a pseudocell of $(T_2O_4Na)_n$, (L= 12.264 $^{\AA}$, N= 84) and a true-cell of (SiAlO$_4Na)_n$. (L= 24.528 $^{\AA}$, N= 672), where T is Si or Al. The radial and bond angle distribution functions of T(Si, Al)-O-T(Si, Al) and diffusion coefficients of T and O were reported at various temperatures. For the true-cell model, the melting temperature is below 1500 K and probably around 1000 K, which is about 600-700 K lower than the pseudocell model. Although it took more time (about 30 times longer) to obtain the molecular trajectories of the true-cell model than those of the pseudocell model, the true-cell model gave more realistic structural transition for the A-type zeolite, which agrees with experiment.

식물에 미치는 방사성 동위원소 S35의 영향에 대하여 (제3보) 발아호밀의 Amylase Activity 및 생장에 미치는 온도의 영향에 대하여 (Effects of absorbed radioactive sulfur (S35) in plant cell.(III) Effects of temperatures on amylase activity and growth of rye seedlings grown in solution of S35)

  • 홍순우
    • Journal of Plant Biology
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    • 제11권1호
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    • pp.1-6
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    • 1968
  • The effects of the different temperatures on the amylase activity and growth rate of the rye seedling grown in the solutions containing radioactive sulfur- 35 were studied. The amylase activity of the coleoptiles obtained from the seedlings grown in the solutions of S-35, at 14$^{\circ}C$, appeared to be strongly stimulated in comparison to the control, but the culture temperatures of 22$^{\circ}C$ and 3$0^{\circ}C$ showed the decrease in the amylase activity. The amylase activity of the grains treated with the low intensity of the ratioactive material didn't show clear changes, at any culture temperatures, but the amylase activity of the grains treated with the high intensity of S-35, 50$\mu$c, showed definite decline at the elevated culture temperature, 3$0^{\circ}C$. Similar effects was also found in the growth of the seedlings. However, we would consider the effects of the radioactive materials on the acticity of the amylase and the growth of the seedlings are resulted from the accumulation of the much amount of the radioactive materials, and this accumulation rate depends upon actually the elevation of the culture temperature.

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