• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.9-13
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• 1990

연산오골계의 위장관에 존재하는 somatostatin cell의 분포상태와 출현빈도를 알아보기 위해 면역조직화학적 방법을 이용하여 광학현미경적으로 관찰하였던 바 다음과 같은 결과를 얻었다. Somatostatin cell은 유문부에 밀집하여 출현하였으며, 그 다음으로 선위의 복합선에서 다수가 관찰되었다. 그리고 십이지장에는 소수가, 공장과 근위에는 드물게 분포하였으며, 회장 맹장 및 직장에서는 관찰되지 않았다. 또한 somatostatin cell은 부위에 따라 그 분포양상의 차이가 인정되었다.

• International Journal of Oral Biology
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• v.32 no.2
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• pp.45-49
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• 2007

In this study, the cytotoxicity of commonly used local anesthetics was evaluated on odontoblasts which are essential for pulpal homeostasis in vitro. Local anesthetics, such as articaine, bupivacaine, levobupivacaine, lidocaine, mepivacaine, prilocaine, and procaine, were tested on the odontoblast cell line, MDPC-23. The concentration-and time-dependent cytotoxic effects of local anesthetics on odontoblasts were measured by MTT assay. Among local anesthetics treated for 18 h, only bupivacaine significantly showed cell death in a concentration-($LC_{50}=1.2mM$) and time-dependent manner. To confirm cell death induced by bupivacaine, the observation of cell morphology and FACS using Annexin V and propidium iodide (PI) staining were performed. As a result of Annexin V and PI staining, as well as the morphological change, only bupivacaine induced apoptotic cell death on odontoblasts when compared with levobupivacaine and lidocaine. These results suggest that bupivacaine might affect normal pulpal integrity even after uneventful local anesthesia.

• Applied Microscopy
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• v.25 no.1
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• pp.86-95
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• 1995

This study was accomplished to investigate the ultrastructure of mucous glands in dorsal skin of frog (Rana catesbeiana) by means of scanning and transmission electron microscopes. The dorsal skin of Rana catesbeiana is composed of epidermis and dermis. The cutaneous mucous glands consist of inner glandular epithelial cells and outer myoepithelial cells. Glandular epithelial cells are divided into four types by the microscopic ultrastructure; ER-rich cell, round secretory granule-containing cell, foam-like granule mass-containing cell, mitochondria-rich cell. Myoepithelial cell has a long elliptical nucleus and filled with fibrous materials in the cytoplasm. As a result of scanning microscopic observation, the surface of dorsal skin is covered with cutaneous protrusions. The opening sites of the mucous glands are irregularly distributed in dorsal skin.

• Nuclear Engineering and Technology
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• v.10 no.1
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• pp.26-36
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• 1978

Numerical computations of radioactivities and decay energies in a spent fuel have been carried out for designing of a hot cell. Optimum wall and window thicknesses that can preserve spent fuel rods for experimental purposes are estimated with burnup rate of 33,000 MWD/T(U) which is nearly maximum from a pressurized water reactor such as the Go-Ri Unit 1. Before putting the spent fuels into a hot cell, it is assumed for thickness estimates of shield materials that they are cooled in a storage tay for several lime intervals. Considered are various types of shield materials through which changing the distances from a source to an observation point is also made.

• Proceedings of the Korean Vacuum Society Conference
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• 1997.07a
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• pp.173-176
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• 1997

A numerical analysis of the micro-discharge in an AC pplasma dispplay cell has been made using time-deppendent, 2-dimensional multi-fluid equations to understand the discharge pphysics of He-Xe discharge. The time deppendent distribution of the electron tempperature, densities of electrons, various ions and excited sppecies, and the effects fo the wall charge accumulated on the dielectric surface are obtained and comppared with the results of direct observation of time deppendent behavior of VUV and visible sppectra from single discharge cell observed using a gated, image intensified CCD to elucidate the discharge physics.

• 한국정보디스플레이학회:학술대회논문집
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• 2003.07a
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• pp.756-759
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• 2003

We measure the spatiotemporal wall charge distributions on sustain and address electrodes during reset period in an ac PDP cell using the longitudinal electro-optic amplitude modulation method. We apply several reset waveforms like as ramp, exponentially growing and high voltage pulse, and compare the wall charge characteristics on address electrode as well as sustain electrodes for each reset waveforms.

• Mycobiology
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• v.28 no.1
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• pp.47-50
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• 2000

The capability of higher ascomyceteous fungi to cause typical soft-rot decay for wood under laboratory conditions is reviewed and discussed. Fungi tested were extremely active in the decomposition of timbers. Scanning electron micrographs illustrated typical soft-rot decay pattern of higher wood decay ascomycetes, with the exception of H. trugodes that caused white-rot decay. Most of the fungi tested could be grouped as soft-rot fungi that showed typical soft-rot type II. Hypha confined primarily to the resin canals in softwoods or vessel elements in hardwoods and spread tracheid to tracheid via pits of cell wall to cell wall with mechanical force.

• BMB Reports
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• v.40 no.6
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• pp.1009-1015
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• 2007

In this communication, we report the efficacy of $\beta$-carotene towards differentiation and apoptosis of leukemia cells. Dose ($20{\mu}M$) and time dependence (12 h) tests of $\beta$-carotene showed a higher magnitude of decrease (significance p < 0.05) in cell numbers and cell viability in HL-60 cells than U937 cells but not normal cell like Peripheral blood mononuclear cell (PBMC). Microscopical observation of $\beta$-carotene treated cells showed a distinct pattern of morphological abnormalities with inclusion of apoptotic bodies in both leukemia cell lines. When cells were treated with $20{\mu}M$ of $\beta$-carotene, total genomic DNA showed a fragmentation pattern and this pattern was clear in HL-60 than U937 cells. Both the cell lines, on treatment with $\beta$-carotene, showed a clear shift in $G_1$ phase of the cell cycle. In addition the study also revealed anti-oxidant properties of $\beta$-carotene since there was reduction in relative fluorescent when treated than the control at lower concentration. Collectively this study shows the dual phenomenon of apoptosis and differentiation of leukemia cells on treatment with $\beta$-carotene.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6533-6535
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• 2013

Background: The purpose of this study was to evaluate a new type of tumor biomarker, eukaryotic elongation factor 2 (eEF2), in serum for the early diagnosis, confirmative diagnosis as well as assessment of treatment of non-small cell lung cancer (NSCLC). Methods: 130 patients with NSCLC and 50 healthy individuals undergoing physical examination in our hospital provided the observation and healthy control groups. An enzyme linked immune sorbent assay (ELISA) method was applied to determine serum eEF2 levels. Serum neuron specific enolase (NSE) and squamous cell carcinoma antigen (SCC) levels in the observation group were assessed with an automatic biochemical analyzer. Results: The median levels of eEF2 in the serum of NSCLC patients was found to be significantly higher than the healthy control group (p < 0.01) and it was markedly higher in stages III, IV than stages I, II (p < 0.05). eEF2 was higher with tumor size ${\geq}2$ cm than <2 cm (P< 0.01). Furthermore, two weeks after surgery patients showed a significant trend for eEF2 decrease (p < 0.05). Conclusions: The eukaryotic elongation factor 2 (eEF2) has certain clinical values for early diagnosis, verification, and prognosis as well as classification of lung cancer patients.

• Journal of Environmental Science International
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• v.9 no.4
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• pp.331-337
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• 2000

The variation of microorganisms (activated slude, Saccharomyces cerevisiae, Aureobasidium pullulans) caused by the biosorption of $Pb^{2+}$ was observed by TEM and microscope. By the TEM observation of S. cerevisiae, the plasmolysis and lysis of cell wall or cell membrane were occurred by the penetration of $Pb^{2+}$ into the inner cellular region. However, in the case of A. pullulans, the plasmolysis and lysis of cell wall or cell membrane were not occurred because of the prevention of $Pb^{2+}$ penetration by the extracelluar polymeric substances (EPS). A flocculation of microorganisms, in the case of A. pullulans, was observed by the $Pb^{2+}$ accumulation after 3~4 h and the color was changed from white to black after 1 day. The flocculation of activated sludge was improved by the accumulation $Pb^{2+}$ after 1 h, however, the floc was broken up and the settling efficiency decreased after 1 day.