• Journal of Korea Multimedia Society
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• v.20 no.5
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• pp.740-747
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• 2017

A fast cell contour extraction method using CUDA parallel processing technique is presented. The cell contour extraction is one of important processes to analyze cell information in pathology. However, conventional sequential contour extraction methods are slow for a huge high-resolution medical image, so they are not adequate to use in the field. We developed a parallel morphology operation algorithm to extract cell contour more quickly. The algorithm can create an inner contour and fail to extract the contour from the concave part of the cell. We solved these problems by subdividing the contour extraction process into four steps: morphology operation, labeling, positioning and contour extraction. Experimental results show that the proposed method is four times faster than the conventional one.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.932-937
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• 2004

In situ extraction by organic solvent was studied in order to improve the recovery yield of hydrocarbon from the culture of Botryococcus braunii, a green colonial microalga. When the solvent mixture of octanol as an extractive solvent and n-octane as a biocompatible solvent was added to a two-phase column, the algal growth was seriously inhibited, even at a low concentration of polar octanol. Therefore, a two-stage cell-recycle extraction process was proposed to improve the contact area between the organic phase and the aqueous phase. The hydrocarbon recovery with in situ cell-recycle extraction showed a three-fold increase (57% of cell) in yield over that with two-phase extraction. In addition, over 60% of the hydrocarbon could be recovered without serious cell damage by downstream separation when this process was applied to the culture broth after batch fermentation.

• Journal of Institute of Control, Robotics and Systems
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• v.14 no.5
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• pp.432-437
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• 2008

This paper proposes a defect cell extraction algorithm for TFT-LCD auto-repair system. Auto defect search algorithm and automatic defect cell extraction method are very important for TFT-LCD auto repair system. In the previous literature[1], we proposed an automatic visual inspection algorithm of TFT-LCD. Based on the inspected information(defect size and defect axis, if defect exists) by the automatic search algorithm, defect cells should be extracted from the input image for the auto repair system. For automatic extraction of defect cells, we used a novel block matching algorithm and a simple filtering process in order to find a given reference point in the LCD cell. The proposed defect cell extraction algorithm can be used in all kinds of TFT-LCD devices by changing a stored template which includes a given reference point. Various experimental results show the effectiveness of the proposed method.

• KSBB Journal
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• v.15 no.4
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• pp.346-351
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• 2000

Several solvents or combinations of solvents were tested for the extraction of wet or dried biomass at different extraction mode from plant cell cultures. Methanol gave the highest paclitaxel recovery with the least amount of solvent usage. before extraction drying of biomass wass helpful to decrease solvent usage in extraction step./ in this case drying method was very important to obtain high yield from dried biomass. In thid mode of operation counter-current extraction process can be able to decrease solvent usage but paclitaxel recovery was almost same with both batch and counter-current mode of operation. The number of extraction times was at least four to obtain high yield(>99%) from cell and one to obtain highyield(>96%) from cell debris in batch mode. Equilibrium (i.e. the ratio of paclitaxel in biomass to paclitaxel in the extraction solvent) was reached within 5 minutes. The minimum methodal concentration (90%) and solvent amount(biomass : solvent=1 Kg : 1L) are enough to obtain high yield(>98%) for extraction from biomass.

• Journal of Applied Biological Chemistry
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• v.56 no.3
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• pp.171-177
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• 2013

Efficient extraction of total proteins from soil microorganisms is tedious because of small quantity. In this regard, an improved method for extraction of whole cell proteins is developed from soil microorganisms, Saccharomyces cerevisiae and Pichia pastoris. of which the cell wall are very strong. Pretreatment with NH4OH prior to the final extraction using NaOH/SDS was tried under the basis that ammonium ion was possible to enhance the permeability and/or to weaken the yeast cell walls. The pre-treatment of yeast cells with NH4OH drastically enhanced the protein extraction when it was compared with control (without NH4OH pre-treatment). At the pre-treatment of 0.04 N NH4OH at pH 9.0, about 3 fold of proteins was obtained from p. pastoris. Ammonium hydroxide appears to penetrate into the yeast cell walls more readily at basic pH. The effect of NH4OH pretreatment was pH dependent. The methods developed in this experiment might be applicable for an effective extraction of yeast proteins for the purpose of biochemical studies, especially proteomic analysis.

• Journal of the Korean Home Economics Association
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• v.39 no.4
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• pp.49-59
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• 2001

Natural red and yellow dyestuff was extracted from safflower (Carthamus tinctorius Linnaeus) by a new process of cellulase extraction compared with the conventional hot water extraction. Dyestuffs were extracted from safflower easily and repeatedly by means of cellulose as safflower cell wall destroyer. It means that new dyestuff extraction by cellulase improves not only yields of dyestuff from safflower successfully but also the rate of repetition of extraction. From the above experiments, the conclusions of this study were summarized as follows. 1. The optimum conditions of dyestuff extraction from safflower by general extraction method were that the solvent was the water of pH 6.0 on yellow dyestuff and 3% $K_2CO_3$ solution on red dyestuff, extraction temperature was $55^{\circ}C$, and extraction time was 30 min. 2. Among various cellulase, the NOVO cellulase was the best cell wall destroyer of safflower and finally produced the largest amount of dyestuff from safflower by cellulase extraction method. 3. The optimum conditions of dyestuff extraction by cellulase extraction method were conducted on 10 unit of cellulase per gram of safflower at $100m{\ell}$ water of pH 5.0 at $50^{\circ}C$ for 30 min.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.847-852
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• 2007

This study investigated an efficient method for the extraction of astaxanthin from the red yeast Xanthophyllomyces dendrorhous. The extraction process comprised three steps: 1) cultivating the yeast; 2) treating the yeast culture suspension with microwaves to destroy the cell walls and microbodies; and 3) drying the yeast and extracting the astaxanthin pigment using ethanol, methanol, acetone, or a mixture of the three as the extraction solvent. Ultimately, various treatment tests were performed to determine the conditions for optimal pigment extraction, and the total carotenoid and astaxanthin contents were quantified. A frequency of 2,450 MHz, an output of 500 watts, and irradiation time of 60 s were the most optimum conditions for yeast cell wall destruction. Furthermore, optimal pigment extraction occurred when using a cell density of 10g/l at $30^{\circ}C$ over 24 h, with a 10% volume of ethanol.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.111-117
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• 2010

The purpose of this study was to investigate the effect of garlic(Allium sativum L.) extracts with extraction conditions on antioxidant and anticancer activity. The extracts prepared for garlic by hot temperature extraction (HG), low temperature extraction (LG), UMPM extraction (UG), fermentation (FG) and black garlic hot temperature (BG) method. Content of total polyphenol compound was the BG higher than other extracts. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manners, and the activity of BG and UM was significantly higher than LG and FG. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. All extracts does not have any toxic effects in macrophages(Raw 264.7). And UG inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of LG and UG on HT 1080 cell was indicated a significantly inhibition activity. These results suggested that UG (UMPM extraction of garlic) have activities of antioxidant, anticancer effects.

• KSBB Journal
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• v.23 no.4
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• pp.281-284
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• 2008

A simple and efficient microwave-assisted extraction procedure was developed and optimized for the extraction of paclitaxel from the plant cell cultures of Taxus chinensis. The biomass, immersed in a methanol-water mixture, was irradiated with microwaves in a closed-vessel system. The microwave-assisted extraction was compared with the existing conventional solvent extraction in terms of yield, extraction time, and solvent consumption. The use of microwave energy allows rapid recovery of paclitaxel from biomass and dramatically reduces extraction time and solvent usage compared to conventional solvent extraction. The paclitaxel was completely extracted from biomass by microwave-assisted extraction for 3 min at $50^{\circ}C$, for 6 min at $30^{\circ}C$ and $40^{\circ}C$, respectively.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.525-529
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• 1990

Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400$\mu g$ shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857$\mu g$ shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.