• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1280-1284
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• 2003

The number of protozoa and VFA content in the rumen fluid, in situ disappearance and turnover rate were examined with four rumen-fistulated cattle given either sugarcane stalk or Ruzi grass hay in order to clarify the manner of rumen digestion of sugarcane stalk. Cattle were given either sugarcane stalk or Ruzi grass hay at 1.0% of body weight level with commercial concentrate feed. Feeding sugarcane stalk reduced acetate content and increased propionate and butyrate contents in rumen fluid. While rapidlysoluble fraction of sugarcane stalk was 42%, the insoluble but potentially degradable fraction was only 17%. This clearly showed that sugarcane stalk mainly consisted of water soluble fraction (i.e. sugar) and tough fiber (i.e. bagasse). The ruminal degradation rate of both Ruzi grass hay and sugarcane stalk was lower in the animal given sugarcane stalk in comparison with those given Ruzi grass hay. While the turnover rate of liquid phase was about 50% higher in the animals given sugarcane stalk than in the animals given Ruzi grass hay, that of the solid phase was about 40% lower in the animals given sugarcane stalk. The effective degradability of DM of sugarcane stalk was higher than that of Ruzi grass hay. Sugarcane would be a promising roughage for ruminants in the tropics especially, in the dry season.

• 한국동물위생학회지
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• 제45권2호
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• pp.117-124
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• 2022

In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treatment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Brucella abortus, Bacillus anthracis, Clostridium chauvoei, Clostridium perfringens, Escherichia coli, and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90℃ for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

• 한국초지조사료학회지
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• 제19권3호
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• pp.203-210
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• 1999

가축액비의 영양성분 중 질소(N)는 환경적인 면과 사초수량을 고려했을 때 잠재력이 가장 높다. 본 연구는 추파용 연맥에 가축액비의 N 함량을 기준으로 시용수준을 달리하여 시용했을 때 추파용 연맥(Avena saliva L.)의 무기물 함량 및 질산태질소 함량 그리고 토양화학성분 변화를 구명하고자 7처리(화학비료표준구 $120kg\;N\;ha^{-1}$, 우분액비구 120, 240, $360kg\;N\;ha^{-1}$ 돈분액비구 120, 240, $360kg\;N\;ha^{-1}$)를 난괴법 3반복으로 배치하여 1997년 수원에서 수행하였다. 대조구와 비교하여 추파용 연맥의 무기물 함량은 액상분뇨의 시용량이 증가됨에 따라 증가되는 경향을 보였다. 추파용 연맥의 질산태질소 함량은 $1,881-2,605mg\;kg^{-1}$의 범위를 보였으며, 금비구>우분액비 $360kg\;N\;ha^{-1}$구>돈분액비 $240kg\;N\;ha^{-1}$구 순이었다. 액상분뇨의 시용량이 증가될 수록 토양중 치환성 양이온 함량이 높아지는 경향을 보였다. 토양중 전질소 및 무기태질소량은 액상분뇨 시용량에 따라 달랐는데 그 중에서도 돈분액비 $360kg\;N\;ha^{-1}$ 구에서 가장 높았다. 토양침투수중 질산태질소 농도는 시험기간중 심한 변이를 나타내지 않았다. 이상의 결과로 볼 때 토양중 질소량은 돈분액비 $360kg\;N\;ha^{-1}$구, 돈분액비 $240kg\;N\;ha^{-1}$ 순이었다.

• 한국환경과학회지
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• 제22권12호
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• pp.1661-1669
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• 2013

This study conducted a laboratory simulation using artificial and natural rainfall in order to investigate the runoff characteristics of livestock resources through the analysis of the surface runoff water and infiltration water by rainfall intensity and fertilization level. Cattle manure and pig liquid fertilizer was used as livestock resource. As a result of this study, it was observed that the surface runoff occurred over 32 mm/hr rainfall intensity, and flow rate of the surface runoff water and the runoff ratio of contaminant parameters from livestock resource was increased, as rainfall intensity was stronger. With doubled fertilization level, T-N increased in compost and the amount of $COD_{Mn}$ runoff also considerably increased in liquefied fertilizer. In the case of natural rainfall, the runoff ratio of T-P clearly increased in compost and the T-N of final surface runoff ratio in compost and liquefied fertilizer was ranged from 0.13047 to 0.13623 with stronger rainfall intensity.

• 한국습지학회지
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• 제13권3호
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• pp.465-470
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• 2011

액비와 퇴비 형태로 발생되어지는 양돈 분뇨는 발생량의 대부분이 농지에 적용되어지고 있다. 과잉으로 적용된 양돈 퇴, 액비는 수계의 영양소 과잉의 주원인으로 알려지고 있는데 양돈 퇴, 액비의 질소, 인 물질이 농지로부터 지표수와 지하수로 전달되어지는 경로를 파악하는 것이 본 연구의 주목적이다. 인공 강우 시험을 통해 농지로부터 양돈 퇴비와 액비가 각각 적용되어진 농지에 대해 지하수와 지표수로 유출되는 양을 조사하였는데 질소의 경우 지하수로 유출되는 부분이 가장 많은 반면 인의 경우 토양에 흡착되는 부분이 가장 많은 것으로 나타났다. 토양에 대한 인의 평균 흡착 양은 21.5 mg P/kg soil 로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.13-17
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• 1993

This study was conducted to determine the cost of production of semen and the rate of conception for artificial insemination in eight randomly selected districts of Bangladesh. A sample of 640 adopting farms were investigated. Results indicate that the cost of liquid semen per dose on full-cost and cash cast basis were Tk. 16.17 and Tk. 14.48, while the cost of locally produced exotic semen and imported semen were Tk. 31.25 and Tk. 110.00 respectively. The cost of liquid semen per insemination, per conception and per progeny on full-cost were Tk. 26.65, Tk. 50.64, and Tk. 56.27 respectively and on cash cost were Tk. 23.88, Tk. 45.37 and Tk. 50.41 respectively. The average cost of semen paid by the farmers was Tk. 14.00 and it was higher in urban areas than in rural areas. Out of the cost of A.I. centres, government had subsidized 92.16 percent and the rest 7.84 percent accrued as returns. About 40 percent of the produced semen was lost in the system which could not be used at all. The conception rate on first insemination was 53.6 percent. The rate increased to 73.2 percent upto the last insemination. The difference in conception rate between liquid semen (69.97%) and frozen semen (70.48%) was not statistically significant.

• 한국유기농업학회지
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• 제20권4호
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• pp.669-685
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• 2012

본 연구는 간척농경지에 경 축 순환단지 구축을 목적으로 2년 3작기 작부체계로 혐기소화액비를 시용하고 사료작물 재배를 실시하여 수량반응과 사료가치분석을 하였으며, 이를 토대로 연간 한우 사육 용량을 추정 하였다. 2년 3작기 동안의 사료작물의 생산성은 전체 혐기소화액비 시비구에서 대조토양에 비해 간척지 토양에서 약 64~76% 수준을 보였으며, 사료 작물별 수확량은 보리 > 옥수수 > 수단그라스 순으로 나타났다. 사료작물의 상대적 사료가치(RFV)는 옥수수가 88~106%, 청보리는 90~111%, 수단그라스는 91~113%이었으며, 한우의 사육용량을 추정 하였을 때 1년 2작기 옥수수-청보리 작부체계에서 5.8~8.6 두/년/ha, 청보리-수단그라스 작부체계에서는 4.8~6.7 두/년/ha로 추정되었고, 혐기소화액비 시비율 200% 처리구에서 가장 높은 연간 한우 사육용량을 보였다. 따라서 결론적으로 간척지토양 같은 조건 불리지라 하더라도 가축분뇨액비를 시용하여 사료작물을 재배하는 것은 생초수량은 감소하였지만 연간한우 사육용량과 사료가치 면에서 크게 뒤지지 않는 결과를 보여 주었다.

• 한국축산식품학회지
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• 제34권5호
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• pp.656-664
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• 2014

The purpose of this study was to analyze myosin heavy chain (MHC) isoforms in bovine longissimus thoracis (LT) muscle by liquid chromatography (LC) and mass spectrometry (MS). LT muscles taken from Hanwoo (Korean native cattle) steer (n=3) used to separate myosin bands by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The peptide queries were obtained from the myosin bands by LC-MS/MS analysis following in-gel digestion with trypsin. A total of 33 and 43 queries were identified as common and unique peptides, respectively, of MHC isoforms (individual ions scores >43 indicate identity or extensive homology, p<0.05). MHC-1 (IIx), -2 (IIa), -4 (IIb), and -7 (slow/I) were identified based on the Mowse score (5118, 3951, 2526, and 2541 for MHC-1, -2, -4, and -7, respectively). However, more analysis is needed to confirm the expression of MHC-4 in bovine LT muscle because any query identified as a unique peptide of MHC-4 was not found. The queries that were identified as unique peptides could be used as peptide markers to confirm MHC-1 (14 queries), -2 (8 queries), and -7 (21 queries) in bovine LT muscle; no query identified as a unique peptide of MHC-4 was found. LC-MS/MS analysis is a useful approach to study MHC isoforms at the protein level.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.559-563
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• 2012

An analytical method for the simultaneous determination of veterinary medicines (amprolium and decoquinate) in cattle and chicken's muscle by HPLC/UV-vis was established. Samples were extracted by a HLB (Hydrophilic-Liphophilic Balance) cartridge with acetonitrile and methanol. Prior to HPLC injection, a mixture solvent (Water:MeOH, 1:1) was utilized as a reconstitution solvent. Chromatographic separation was achieved with a C18 column ($250{\times}4.6mm$, $5{\mu}m$) using gradient elution with 20 mM HFBA and MeOH:ACN (1:1.8). The calibration curves from the spiked blank matrix showed good linearity (above $r^2$=0.997) in the concentration range of $0.13-12.0mg\;kg^{-1}$. The relative recovery (accuracy) and limit of quantitation (LOQ) were in the range of 78.5-107.1% and $0.13-0.42mg\;kg^{-1}$, respectively. The developed method can be used to determine under the MRL (Maximum Residue Limits) levels of veterinary medicines in animal tissues.

• Asian-Australasian Journal of Animal Sciences
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• 제10권3호
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• pp.329-332
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• 1997

A study on the conception rate of 885 cows and heifers bred under artificial insemination (AI) and natural service was carried out at AI sub-centers. A high conception rate was observed at first service in all breeding methods. It was revealed that the conception rates in AI with liquid semen (LS) were found to be 25.08, 18.58, 12.69, and 2.48% after 1, 2, 3 and 4 services, respectively. Inseminations of cattle with frozen semen (FS) were found to achieve 42.15, 14.46, 5.85, and 2.46% conception rates after 1, 2, 3, and 4 services, respectively. In natural service, conception rates were found to be 62.45, 6.75, 5.91, and 4.64%, respectively, after 1, 2, 3, and 4 services. Insemination of cows and heifers at mid-cycle had significantly (p < 0.05) higher conception rate as compared to early and late cycles. The existing AI machineries and breeding management need to be improved to achieve higher conception rate of dairy cattle under AI.