• 한국수정란이식학회지
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• 제21권3호
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• pp.191-198
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• 2006

본 연구는 $2001{\sim}2005$년(5년간) 제주도에서 사육중인 제주 한우와 흑우를 체내 수정란의 생산과 수정란 이식 기술을 통하여 조기 증식하고자 실시하였다. 한우 고등 등록우 286두와 흑우 경산우 69두(총 355두)에 대하여 발정 주기에 관계없이 CIDR를 질내에 삽입 후 7일째부터 성선 자극 호르몬($Folltropin^{(R)}-V$) 400 mg을 50 mg씩 균등하게 나누어 4일간 12시간 간격으로 근육주사하였다. 투여 6회째에 CIDR를 제거하였으며, 동시에 $PGF_{2{\alpha}}$ 25mg을 근육 주사하여 과배란을 유기하였다. 공란우의 인공수정은 $PGF_{2{\alpha}}$투여 후 발정을 확인하고 12 시간 간격으로 3회 인공 수정하였으며, 2회 인공 수정시 GnRH $250{\mu}g$을 근육 주사하였다. 수정란 회수는 1차 인공 수정 후 $7{\sim}8$일째에 비외과적 방법으로 채란하였다. 수란우는 CIDR를 7일간 삽입하였다가 제거하는 동시에 $PGF_{2{\alpha}}$ 25 mg을 근육 주사하여 공란우와 발정을 동기화 시켰다. 수정란 이식은 발정이 동기화된 수란우 1,219두에 대하여 6명의 수정란 이식 시술자가 비외과적 방법으로 황체가 존재하는 자궁각에 이식하였다. 한우 및 흑우 공란우의 평균 회수 총 난자수 및 이식 가능 수정란 수는 각각 7.4개 및 4.7개 였으며, 회수 총 난자수는 2 품종간에 차이가 없었으나, 이식 가능 수정란 수는 한우(5.0개)가 흑우(3.5개)에 비해 많았다(p<0.05). 공란우의 반복 과배란 처리에 따라 흑우에서는 과배란처리 두수 대비 수정란 채란우 비율이 감소되었으며(p<0.05), 한우에서는 회수 총 난자수 및 이식 가능 수정란 수의 감소가 나타났다(p<0.05). 과배란 처리 계절에 따른 수정란 회수 성적은 여름(5.6개)이 겨울(2.9개)에 비해 이식 가능 수정란의 수가 많았다(p<0.01). 수정란 이식 후 수태율은 평균 40%를 나타내었으며, 공란우의 품종간 차이가 없었으며, 이식년도가 2001년에서 2004년으로 경과될 때 수태율이 증가하였으며(p<0.05), 수정란 이식 시술자에 따른 수태율의 차이가($33.0{\sim}45.9%$) 인정되었다(p<0.01). 본 연구의 결과는 제주 한우 및 흑우에서 CIDR를 이용한 과배란 처리 및 수란우의 발정동기화 처리는 한우 체내 수정란의 안정적인 생산과 수란우의 준비가 가능함을 보여 주었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권2호
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• pp.71-78
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• 2000

The recent progress od DNA technologies including DNA fingerprinting (DFP) and random amplified DNA polymorphism (RAPD) analysis make it possible to identify the specific genetic trits of animals and to analyze the genetic diversity and relatedness between or withinspecies or populations. Using those techniquse, some efforts to identify and develop the specific DNA markers based on DNA polymorphism, which are related with economic traits for Korean native animals, Hanwoo(Korean native cattle),Korean native pig and Korean native chicken, have been made in Korea for recent a few years. The developed specific DNA markers successfully characterize the Korean native animals as the unique Korean genetic sources, distinctively from other imported breeds. Some of these DNA markers have been related to some important economic traits for domestic animals, for example, growth rate and marbling for Honwoo, growth rate and back fat thinkness fornative pig, and growth rate, agg weight and agg productivity for native chicken. This means that those markers can be used in important marker-assised selection (MAS) of Korean native domestic animals and further contribute to genetically improve and breed them.

• Asian-Australasian Journal of Animal Sciences
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• 제24권6호
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• pp.757-765
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• 2011

Fatty acid composition of beef intramuscular tissue is an important trait because high proportions of mono-unsaturated fatty acid are related to favorable beef flavor. In this study, we investigated the effects of genetic factors, such as stearoyl-CoA desaturase (SCD), on beef carcass traits, including fatty acid composition, in the Hanwoo. Analysis of fatty acids in Hanwoo was performed using a breed raised in Gyeonbuk province (n = 395). Compared to the homozygote, the GA, CT, and CT genotypes of exon 5 in the SCD polymorphism showed a higher content of oleic acid (p<0.05) and higher contents of mono-unsaturated fatty acid (p<0.05) and marbling scores (p<0.05) in intramuscular fat. Results of haplotype analysis showed a significant presence of unsaturated fatty acids and marbling score in the $ht1^*ht2$ and $ht2^*ht2$ groups (p<0.05). Furthermore, haplotype effects more powerful than a single gene were also observed. These ht1 and ht2 types also showed a significant difference in unsaturated fatty acids and marbling score, affecting beef flavor in the Hanwoo groups. Therefore, it can be inferred that the ht1 and ht2 types might be valuable new markers for use in improvement of Hanwoo.

• 한국수정란이식학회지
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• 제31권2호
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• pp.137-143
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• 2016

This study was conducted to evaluate a visual surveillance system. The advancement of recording technology and network service make it easy to record and transfer the videos. Moreover, progressed recognition technology help to make a distinction each other. Cows show distinguishing behaviors during their estrus period. The mounting is one of the behaviors. The result was different depending on the breed of the cows and the size of the farm. In the case of Korean native cattle, the estrus detection rate was 71.15%, however, dairy cows, the estrus detection rate was 39.38%. At the farms having below 6 modules, the estrus detection rate was 87.41%. On the other hand, at the farms having over 6 modules, the estrus detection rate was 77.78%. With the proper progress, the visual surveillance system can be used to detect heat detection.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.549-553
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• 1993

In 1985, fifty Jersey cows were imported to Pakistan from United States of America (USA). This paper compares milk production and reproduction performance of imported cows and their daughters under USA and Pakistan conditions. The imported cows which had yielded 5,858 kg, of milk (305 d lactation) in the USA produced only 3,192 kg, at NARC. The milk production of their daughters averaged 2,617 kg, being 22% lower than their mothers under the same environment at NARC. Year and season of calving had no significant effects on milk production. Average milk production of the first lactation was 10% lower than the mean of the second, third and fourth lactations, but, the differences between lactation means were non-significant. Mean calving interval in the USA was 385 days, whereas the same cows had a longer (p<.01) calving interval of 490 days at NARC. The calving interval of the daughters was longer (452 d; p<.01) than their mothers calving interval in the USA, and shorter (p<.01) than their mothers calving interval in Pakistan. The low age at first calving suggests that locally born Jersey cows were not reproductively disadvantaged by the local environment and nutrition, suggesting the adaptability of this breed in the new environment.

• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.466-469
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• 2002

The data on 512 Hariana cows, progeny of 20 sires calved during period from 1974 to 1993 maintained at Government Livestock Farm, Hisar were considered for the estimation of genetic parameters. The means for first lactation milk yield (FLY), wet average (WA), first lactation peak yield (FPY), first lactation milk yield per day of first calving interval (MCI) and first lactation milk yield per day of age at second calving (MSC) were 1,141.58 kg, 4.19 kg/day, 6.24 kg/day, 2.38 kg/day and 0.601 kg/day, respectively. The effect of period of calving was significant (p<0.05) on WA, FPY and MCI while the effect of season of calving was significant only on WA. Monsoon calvers excelled in performance for all the production efficiency traits. The effect of age at first calving (linear) was significant on all the traits except on MCI. Estimates of heritabilty for all the traits were moderate and ranged from 0.255 to 0.333 except for WA (0.161). All the genetic and phenotypic correlations among different production efficiency traits were high and positive. It may be inferred that selection on the basis of peak yield will be more effective as the trait is expressed early in life and had reasonably moderate estimate of heritability.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1244-1249
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• 2002

Myostatin (growth differentiation factor (GDF)-8), is one member of the transforming growth factor $\beta$ superfamily. Investigations of GDF-8 null mice and double-muscled cattle revealed that GDF-8 has a profound influence upon skeletal muscle growth. Therefore, the GDF-8 effect upon the productive performance of pigs is worth exploring. In the present study, the nucleotide sequences and expression levels of GDF-8 genes in European pigs (Landrace and Duroc) and Asian pigs (Taoyuan and Small-ear) were evaluated. Based upon their genetic background these breeds possess significantly distinct growth rate and muscle productionphenotypes. Our sequence data showed that the nucleotide sequences of European and Asian pigs were 100% similar. Postnatal expression of GDF-8 gene in skeletal muscles, from birth to 12 mo of age, among different breeds was measured. GDF-8 expression levels in the longissimus muscle of neonatal European breed littermates were the highest, however it declined significantly (p<0.05) at 1 and 3 mo, and then increased gradually at 6 to 12 mo. The Asian breeds, however, GDF-8 expression level increased markedly at 3 mo and maintained a constant level thereafter. The results indicate that rather than polymorphism within the GDF-8 functional sequence between European and Asia breeds, it was relative to the gene regulation in postnatal muscle growth.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1093-1101
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• 2003

Reproductive data, such as numbers of days to the first estrus and A.I. service postpartum, number of days to conception, number of A.I. services required for conception, interval between the first estrus and first A.I. service and the average interval of A.I. service in Thai native-Friesian crossbred and pure Friesian dairy cows, were compiled in the National Dairy Training and Applied Research Institute in Chiang Mai, Thailand. The data were analyzed statistically and the effect of milk production on these reproductive traits was investigated. The reproductive efficiency of purebred cows was obviously inferior when compared with crossbred animals, in spite of special care being given to the purebred only in order to alleviate the effect of a tropical climate and provide better feeding. However, the regression analysis between reproductive and lactational parameters revealed a definite antagonistic effect of lactation on reproduction, especially in the purebred cows, which had a larger amount of milk production and longer lactation period. If these effects of lactation were eliminated, there would be no evident difference in reproductive efficiency between purebred and crossbred cows in the conditions of this study. Among the reproductive parameters examined, the number of days to the first estrus and interval between the first estrus and first A.I. service were less affected by breed difference and the magnitude of lactation than other reasons.

• Asian-Australasian Journal of Animal Sciences
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• 제17권7호
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• pp.954-959
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• 2004

In a herd of 24 spring-born steers, plasma leptin and performance of selected purebred (n=5) and backcrossed Hereford (n=5) were compared in a year-round summer grazing and winter feedlot fattening. Bimonthly blood collection and body weight measurement were accomplished. The plasma samples were analyzed for leptin, insulin, total cholesterol, triglyceride, NEFA and glucose. The experimental design utilized one-way ANOVA with breed as the treatment. The purebred obtained higher plasma NEFA (p<0.001) compared to backcross, regardless of seasonal feeding systems (SFS). The backcross showed gradual increase and nonresponsiveness of plasma leptin to SFS. During summer grazing, attenuation of plasma leptin and sudden elevation when shifted to winter feedlot fattening were observed in purebred. Plasma leptin obtained linear relationship with body weight of purebred (r=0.53;p<0.001) and backcrossed Hereford (r=0.49; p<0.01). The purebred and backcrossed Hereford, when shifted to summer grazing, resulted to sustained and restricted daily gain, respectively. Therefore, cattle breeds of higher growth potential exhibit significant elevation of plasma leptin after 400 kg BW, when animal starts to deposit significant body fat.

• 한국동물생명공학회지
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• 제37권2호
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• pp.80-86
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• 2022

The ability to determine the sex of bovine embryos before the transfer is advantageous in livestock management, especially in dairy production, where female calves are preferred in milk industry. The milk production of female and male cattle benefits both the dairy and beef industries. Pre-implantation sexing of embryos also helps with embryo transfer success. There are two approaches for sexing bovine embryos in farm animals: invasive and non-invasive. A non-invasive method of embryo sexing retains the embryo's autonomy and, as a result, is less likely to impair the embryo's ability to move and implant successfully. There are lists of non-invasive embryo sexing such as; Detection of H-Y antigens, X-linked enzymes, and sexing based on embryo cleavage and development. Since it protects the embryo's autonomy, the non-invasive procedure is considered to be the safest. Invasive methods affect an embryo's integrity and are likely to damage the embryo's chances of successful transformation. There are different types of invasive methods such as polymerase chain reaction, detection of male chromatin Y chromosome-specific DNA probes, Loop-mediated isothermal amplification (LAMP), cytological karyotyping, and immunofluorescence (FISH). The PCR approach is highly sensitive, precise, and effective as compared to invasive methods of farm animal embryonic sexing. Invasive procedures, such as cytological karyotyping, have high accuracy but are impractical in the field due to embryonic effectiveness concerns. This technology can be applicable especially in the dairy and beef industry by producing female and male animals respectively. Enhancing selection accuracy and decreasing the multiple ovulation embryo transfer costs.