• Animal Bioscience
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• 제37권6호
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• pp.965-981
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• 2024

Objective: Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene expression at the post-transcriptional level. We compared the relative expression levels of miR-106b, miR-191, and miR-30d in milk to demonstrate the relationship between the content of these miRNAs with protein and fat components of milk in Holstein and Ayrshire cattle. Methods: Milk fat, protein, and casein contents were determined in the obtained samples, as well as the content of the main fatty acids (g/100 g milk), including: saturated acids, such as myristic (C14:0), palmitic (C16:0), and stearic (C18:0) acids; monounsaturated acids, including oleic (C18:1) acid; as well as long-, medium- and short-chain, polyunsaturated, and trans fatty acids. Real-time stem-loop one-tube reverse transcription polymerase chain reaction with TaqMan probes was used to measure the miRNA expression levels. Results: The miRNA expression levels in milk samples were found to be decreased in the first two months in Holstein breed, and in the first four months in Ayrshire breed. Correlation analysis did not reveal any dependence between changes in the expression level of miRNA and milk fat content, but showed a multidirectional relationship with individual milk fatty acids. Positive associations between the expression levels of miR-106b and miR-30d and protein and casein content were found in the Ayrshire breed. Receiver operating characteristic curve analysis showed that miR-106b and miR-30d expression levels can cause changes in fatty acid and protein composition of milk in Ayrshire cows, whereas miR-106b expression level determines the fatty acid composition in Holsteins. Conclusion: The data obtained in this study showed that miR-106b, miR-191, and miR-30d expression levels in milk samples have peculiarities associated with breed affiliation and the lactation period.

• 생명과학회지
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• 제21권4호
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• pp.494-501
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• 2011

소의 Extension (E), Agouti (A) 유전자형과 모색유전양상의 연관관계를 이해하기 위해, 한우와 제주흑우(JBC), 이들 사이에서 생산된 교배후손에서 melanocortin-1 receptor (MC1R)과 agouti signaling protein (ASIP)의 유전자형을 분석하였다. 흑모색의 JBC에서는 세 가지 MC1R 대립인자들($E^D$, $E^+$, e)이 모두 발견되었다. JBC 집단은 한우에서 우점적으로 나타나는 열성동형접합자(e/e)가 발견되지 않았다. 반면, MC1R $E^+$/e 한우는 흑모색이 아닌 갈색이나 적색의 모색을 나타내었다. ASIP 유전자에서, 한우에서는 L1-BT 전위인자의 삽입/결실에 의해 세 가지 유전자형(A/A, A/$A^{Br}$, $A^{Br}/A^{Br}$)이 모두 관찰되었다. JBC 집단에서는 ASIP $A^{Br}$ 대립인자가 드물게 관찰되고, ASIP $A^{Br}/A^{Br}$ 동형접합인 개체들은 발견되지 않았다. ASIP $A^{Br}$ 대립인자를 보유하더라도 한우와 JBC, 이들의 교배후손에서도 agouti-유사 얼룩무늬 모색은 발견되지 않았다. 교배후손의 모색은 Agouti 유전자형과는 무관하고, Extension 유전자형과 직접적으로 연관되어 황-갈색(e/e)과 암-갈/흑색($E^+$/e)으로만 구분되었다. ASIP 유전자형은 아마도 한우 또는 교배 후손에서 모색의 발달에 영향이 없는 것으로 추정된다. 본 연구 결과는 한우, 제주흑우, 이들의 교배후손에서 MC1R 유전자형이 모색 표현형을 결정하며, ASIP 유전자형은 모색의 변이에는 핵심적인 역할을 수행하지 않는다는 것을 시사한다.

• Journal of the Korean Data and Information Science Society
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• 제25권3호
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• pp.533-545
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• 2014

경북지역의 한우농가를 대상으로 한우 사육실태와 육종개량에 대한 농가들의 인식도를 조사 분석하여, 앞으로 사육 기술지도와 한우개량 방향을 개선하는데 기초자료로 이용하고자 설문조사하여 분석한 결과는 다음과 같다. 후보번식우 선발은 '외모, 체형, 혈통등록우' (39.0%)를 고려하여 선발하였으며, 번식우의 종부방법은 '계획교배를 통한 인공수정' (38.6%)을 실시하고 있어 가장 높게 나타났다. 비육밑소 구입시 외관상 가장 관심을 두는 부분은 '체장' (40.7%)부분으로 나타났고, 비육 밑소의 거세는 '생후 6~7개월'이 가장 많이 실시하였고, 한우고급육 생산을 위하여 '고급육생산 프로그램에 준하여 80% 이상 맞춰준다' (51.7%)고 하였다. 그러나 농가들은 비육출하 월령은 '생후 28개월' (35.2%)이 가장 경제적일 것이라고 생각하고 있었다. 이러한 결과는 조사항목에 따라 다소 차이가 있긴 하지만 대체로 조사대상자의 일반적 특성에 따라 학력, 연령, 직업, 가족 노동력에 따라 유의수준 0.05에서 통계적으로 유의한 차이가 있었다. 그러나 비육 후 출하 시에 육질 1+등급 이상 출현율은 '출하두수의 30% 정도' (22.1%)라고 응답하여 가장 높았지만, 조사대상자의 알반적 특성 간에는 유의한 차이가 나타나지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권6호
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• pp.794-799
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• 2003

A study was conducted on 2102 records of 808 crossbred cows of various genetic groups maintained under 'All India Coordinated Research Project on Cattle' at C C S Haryana Agricultural University, Hisar, over 25 years period (1968-1993) with an objective to assess and compare the amount of percent improvement and heterotic effect for different performance traits in various genetic groups produced under this programme. Survivability sharply and significantly declined from 1/2 to $3/4^th$ bred and further from $3/4^th$ to inter-se bred. This may be due to periodic and management differences in addition to the higher level of exotic inheritance and decreased heterotic effect over the filial generations. Jersey and Holstein Friesian crosses among 1/2 breds and their 50% inheritance among $3/4^th$ and inter-se breds had highest improvement and heterosis in reproduction and production traits respectively. Among inter se bred genetic groups, BFH (I) had no recombination loss in SP and CI, while FJH (I), JFH (I) and FBH (I) had on recombination loss in AFC, LY, LL and PE. The crossbreeding of zebu cows with exotic breeds brings about spectacular improvement in comparison to the performance of zebu breed, while conventional selection over several generation would lead to only modest improvement. In addition to additive effect, there was sufficient heterosis in Jersey crosses for reproduction and Holstein Friesian crosses for production performance. Three breed crosses with exotic inheritance between 50 and 75 percent incorporating genes (25 to 50%) from both of these breeds is the best combination for stabilization.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1371-1377
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• 2002

During a 5-yr period, 1994-1998, pre-weaning and weaning data were collected on 591 calves produced by mating either straightbred Jersey, straightbred Limousin or $F_1$ (Limousin${\times}$Jersey) bulls to mature purebred Jersey or Limousin cows. Traits recorded included birth and weaning weight, height, length, girth, fat depth and a measure of muscle (ratio of stifle to hip width expressed as a percentage). All traits were analyzed assuming a model with sire and dam random effects that included effects of year and date of birth, sex, breed and year${\times}$sex interaction. Main effects were generally significant with few exceptions. Direct genetic effects were large for weight, height, girth and muscle with a breed trend from purebred Jersey (small) to purebred Limousin (large). At weaning, the maternal effect of the Jersey dam was positive for weight (10.9${\pm}$4.9 kg), girth (3.7${\pm}$1.0 cm) and muscle (6.0${\pm}$0.9%). Heterosis was highly significant and positive only for fat depth (1.5${\pm}$0.2 mm) with the $F_1$ progeny being the fattest, followed by the backcrosses, then purebred Jersey and purebred Limousin. Also, significant (p<0.001) but negative heterosis was observed for weight, girth and muscle. The change in ranking for fat depth relative to other traits is a reflection of the large heterotic effects relative to direct effects on fat depth. Epistatic effects were not significant on any trait at birth or weaning. This study has indicated the possibility of exploiting the positive heterotic and maternal effects for fat depth and muscularity to meet specific meat quality and quantity demand by consumers.

• 한국축산식품학회지
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• 제42권3호
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• pp.426-440
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• 2022

The bioactive functions of oligosaccharides from human milk have been reported by many studies. Many of oligosaccharides isolated from colostrum and/or milk of dairy animals have been reported to have similar chemical structures with those in human colostrum and/or milk. It has been proved by several studies that the oligosaccharides with similar chemical structure shared common bioactivities. Among domesticated dairy animals, bovine/cattle, caprine/goat, and ovine/sheep are the most commonly used species to isolate oligosaccharides from their colostrum and/or milk. Several studies on the oligosaccharides from goat colostrum and milk have revealed similar properties to that of human milk and possess the highest content of sialyl oligosaccharides (SOS) as compared to other ruminants. Indonesia ranks first in Association of Southeast Asian Nations (ASEAN) for goat milk production. Therefore, goat milk is the second most consumed milk in the country. The most reared dairy goat breed in Indonesia is Etawah Grade. However, oligosaccharides from Indonesia dairy animals including goat, have not been characterized. This is the first study to characterize oligosaccharides from Indonesia dairy animals. The present study was aimed to isolate and characterize oligosaccharides, specifically SOS from the colostrum of Etawah Grade goats by using proton/¹H-nuclear magnetic resonance. The SOS successfully characterized in this study were: Neu5Ac(α2-3)Gal(β1-4)Glc (3'-N-acetylneuraminyllactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6'-N-acetylneuraminyllactose), Neu5Gc(α2-3)Gal(β1-4)Glc (3'-N-glycolylneuraminyllactose), Neu5Gc(α2-6)Gal(β1-4)Glc (6'-N-glycolylneuraminyllactose), Neu5Ac(α2-6)Gal(β1-4) GlcNAc (6'-N-acetylneuraminyllactosamine) and Neu5Gc(α2-6)Gal(β1-4)GlcNAc (6'-N-glycolylneuraminyllactosamine). This finding shows that Etawah Grade, as a local dairy goat breed in Indonesia, is having significant potential to be natural source of oligosaccharides that can be utilized in the future food and pharmaceutical industries.

• 한국축산식품학회지
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• 제43권4호
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• pp.563-579
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• 2023

Myogenesis and adipogenesis are the important processes determining the muscle growth and fat accumulation livestock, which ultimately affecting their meat quality. Hanwoo is a popular breed and its meat has been exported to other countries. The objective of this study was to compare the myogenesis and adipogenesis properties in satellite cells, and meat quality between Hanwoo and Vietnamese yellow cattle (VYC). Same 28-months old Hanwoo (body weight: 728±45 kg) and VYC (body weight: 285±36 kg) steers (n=10 per breed) were used. Immediately after slaughter, tissue samples were collected from longissimus lumborum (LL) muscles for satellite cells isolation and assays. After 24 h post-mortem, LL muscles from left carcass sides were collected for meat quality analysis. Under the same in vitro culture condition, the proliferation rate was higher in Hanwoo compared to VYC (p<0.05). Fusion index was almost 3 times greater in Hanwoo (42.17%), compared with VYC (14.93%; p<0.05). The expressions of myogenesis (myogenic factor 5, myogenic differentiation 1, myogenin, and myogenic factor 6)- and adipogenesis (peroxisome proliferator-activated receptor gamma)-regulating genes, and triglyceride content were higher in Hanwoo, compared with VYC (p<0.05). Hanwoo beef had a higher intramuscular fat and total monounsaturated fatty acids contents than VYC beef (p<0.05). Whilst, VYC meat had a higher CIE a* and total polyunsaturated fatty acids content (p<0.05). Overall, there was a significant difference in the in vitro culture characteristics and genes expression of satellite cells, and meat quality between the Hanwoo and VYC.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.470-474
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• 1998

Data on the first lactation performance traits of $F_1$, $F_2$ and $F_3$ crosses covering the period from 1972 to 1995 of a total of 803 dairy cows of three genetic grades maintained at Livestock Production Research Farm, Indian Veterinary Research Institute, Izatnagar were analysed. Three genetic grades were 1/2 Friesian + 1/2 Hariana (FH), 1/2 Friesian + 1/4 Brown Swiss and 1/4 Hariana (FBH) and 1/2 Friesian+ 1/4 Jersey + 1/4 Hariana (FJH). Age at first calving increased by 7% and 8% in $F_2$ and $F_3$, respectively, over the $F_1$ in FH. The reduction in age at first calving at $F_2$ and $F_3$ levels by 2-7% over the $F_1$ was observed in FBH and FJH. The lactation milk yield of $F_1$, $F_2$ and $F_3$ crosses was $1,943{\pm}100.3$, $2202{\pm}120.5$ and $1,925{\pm}123.2kg$ in FH; $2,014{\pm}76.7$, $2,264{\pm}91.5$ and $2,096{\pm}123.9kg$ in FBH and $2,005{\pm}87.0$, $2,414{\pm}94.4$ and $2,093{\pm}121.1kg$ in FJH, respectively. The lactation milk yield improved by 12-20% in $F_2$ crosses in various genetic grades. The performance of $F_1$ was, however, maintained in FH $F_3$ crosses, it improved by 4% in FBH and FJH $F_3$ crosses. The lactation lengths and calving intervals were nearly the same for $F_1$, $F_2$ and $F_3$ crosses in FH while lactation lengths and calving intervals were reduced by 3-11% in $F_2$ and $F_3$ crosses in FBH and FJH genetic grades. The milk yield/day of lactation length and milk yield/day of calving interval increased by 16-35% in $F_2$ and 2-14% in $F_3$ over the $F_1$ in various genetic grades. It is recommended that a sufficiently large effective population size of these three genetic grades be maintained by inter se matings and rigorous selection of sires so for developing a genetic base population for new breed development.

• 한국동물위생학회지
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• 제43권3호
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• pp.113-128
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• 2020

For estimating the prevalence of bovine infectious disease agents, the pathogens were classified as follows: the digestive disease agents, respiratory disease agents, reproductive disease agents, and tick-borne disease agents. This study covered 81 published papers regarding bovine infectious diseases in Korea that determined the presence of diverse pathogens or the antibodies elicited by the infectious agents in cattle from 2010 to 2019. In total, 59,504 cows were involved in the papers reporting the causative agents in their cases. The disease prevalence for the digestive, respiratory, reproductive, and tick-borne cases was 9.0%, 13.4%, 10.4%, and 7.8%, respectively. Bovine viral diarrhea virus, Escherichia coli, Mycobacterium avium subsp. paratuberculosis, and Eimeria spp were more significantly prevalent in the cows under one-year age than over one-year age. Bovine viral diarrhea virus, Escherichia coli, Mycobacterium avium subsp. paratuberculosis, and Anaplasma spp. were more significantly prevalent in Hanwoo than dairy cattle. Coxiella burnetii, Neospora caninum, and Theilieria spp. were more significantly prevalent in dairy cattle than Hanwoo. Tick-borne disease agents were more prevalent in cows grazing than the case in housing. Our analytic data obtained from this study emphasize the need for more studies on the occurrence of these pathogens according to the breed, age, and the region, to come up with bovine infectious disease control measures in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.1054-1060
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• 2017

Objective: This study was aimed at evaluating effects of cattle breed resources and alternative mixed-feeding practices on meat productivity and emission intensities from household farming systems (HFS) in Daklak Province, Vietnam. Methods: Records from Local $Yellow{\time}Red$ Sindhi (Bos indicus; Lai Sind) and 1/2 Limousin, 1/2 Drought Master, and 1/2 Red Angus cattle during the growth (0 to 21 months) and fattening (22 to 25 months) periods were used to better understand variations on meat productivity and enteric methane emissions. Parameters were determined by the ruminant model. Four scenarios were developed: (HFS1) grazing from birth to slaughter on native grasses for approximately 10 h plus 1.5 kg dry matter/d (0.8% live weight [LW]) of a mixture of guinea grass (19%), cassava (43%) powder, cotton (23%) seed, and rice (15%) straw; (HFS2) growth period fed with elephant grass (1% of LW) plus supplementation (1.5% of LW) of rice bran (36%), maize (33%), and cassava (31%) meals; and HFS3 and HFS4 computed elephant grass, but concentrate supplementation reaching 2% and 1% of LW, respectively. Results: Results show that compared to HFS1, emissions ($72.3{\pm}0.96kg\;CH_4/animal/life$; least squares $means{\pm}standard$ error of the mean) were 15%, 6%, and 23% lower (p<0.01) for the HFS2, HFS3, and HFS4, respectively. The predicted methane efficiencies ($CO_2eq$) per kg of LW at slaughter ($4.3{\pm}0.15$), carcass weight ($8.8{\pm}0.25kg$) and kg of edible protein ($44.1{\pm}1.29$) were also lower (p<0.05) in the HFS4. In particular, irrespective of the HSF, feed supply and ratio changes had a more positive impact on emission intensities when crossbred 1/2 Red Angus cattle were fed than in their crossbred counterparts. Conclusion: Modest improvements on feeding practices and integrated modelling frameworks may offer potential trade-offs to respond to climate change in Vietnam.