• Journal of fish pathology
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• v.6 no.1
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• pp.57-64
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• 1993

The properties and DNA structures of R plasmids differ depending on the species of the fish-pathogens Aeromonas hydrophila, A. salmonicida, Edwardsiella tarda, Enterococcus seriolicida, Pasteurella piscicida and Vibrio anguillarum. However, some R plasmids with the same resistance markers in similar DNA structures were found in A. hydrophila and E. tarda, as well as in A. hydrophila and A. salmonicida. R plasmids from V. anguillarum were classified into three groups according to their DNA structures. The first group was detected before 1977, the second from 1980 to 1983, and the third from 1989 to 1991. R plasmids have been retained within P. piscieida having the same DNA structures and detected at various locations and times. E. seriolicida strains carrying the same R plasmids, which were encoded with resistance to macrolide antibiotics(MLs), lincomycin(LIM), and TC, and to MLs, LIM, and CP. were distributed in yellowtail farms in various districts. The chloramphenicol-resistance(cat) gene of the R plasmids of P. piscicida was classified as CAT type I. The cat of the R plasmids of E, tarda. A. salmonicida was classified as type II. The cat of R plasmids of V. anguillarum was classified into two types. One type detected before 1977, was classified as CAT IV and the other type, detected after 1980, was classified as CAT II. Tetracycline-resistance (tet) V. anguillarum, isolated before 1977 and after 1981, was classified as Tet B and Tet G, respectively. The class D tet gene was widely distributed in R plasmids from fish-pathogens A. hydrophila, E. tarda, P. piscicida, and also V. anguillarum isolated after 1989.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.194-200
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• 1989

The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. vertical bar /sup 35/S vertical bar-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13.

• Current Research on Agriculture and Life Sciences
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• v.11
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• pp.81-89
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• 1993

In order to isolate a mutant which was constitutively expressed in xylA gene, Pxyl-cat-xylA fusion gene was constructed by the insertion of cat gene between xylA promoter and xylA structural gene in pEX13 contained xylA gene. The expression of cat and xylA gene from transformants of xylA mutant DH77 with plasmid pEXC131 containing Pxyl-cat-xylA fusion gene was induced by the addition of 0.4% xylose to media. This results indicated that cat and xylA gene were expressed under control of xylA promoter the presence of xylR gene. We have also isolated constitutive mutant plasmid pEXC131-39 from pEXC131 by trementment with N-methyl-N'-nitro-N-nitrosoguanidine(NTG). cat and xylA gene from pEXC131-39 were constitutively expressed without induction of xylose regardless of xylR gene. Transformants of xylR mutant DH60 with pEXC131-39 also expressed chloramphenicol resistances and xylose isomerase without induction of xylose. This result shows that mutation in region of xylA promoter might make it possible to be constitutively expressed.

• Journal of Microbiology
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• v.38 no.4
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• pp.239-244
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• 2000

The cats gene encodes the major catalase in Sreptomyces coelicolor, whose production increases upon H$_2$O$_2$treatment. Besides the previously identified primary promoter (catApl), a minor promoter (catAp2) was newly assigned by S1 nuclease mapping. The catAp2 transcript was observed transiently upon entry into the stationary phase in liquid culture and upon differentiation on solid plates, whereas the level of catApl transcription did not chance significantly during this growth transition. ThecatApl promoter was transcribed by the major vegetative RNA polymerase holoenzyme containing $\sigma$$\^$HrdB/, whereas the catAp2 was transcribed in vitro by the holoenzyme containing $\sigma$$\^$R/ that is activated under oxidative conditions. The cia-element regulating the H$_2$O$_2$-inducibility of catApl was identified within the 23 bp inverted repeat sequence located between -65 and -43 of the catApl promoter. We roamed this sequence HRE (H$_2$O$_2$-responsive Element). The distal half of the inverted repeat was more crucial for H$_2$O$_2$-dependent induction of the catApl transcript than the proximal half. HRE most likely serves as a binding site for the H$_2$O$_2$-responsive repressor CatR.

• Journal of Microbiology
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• v.39 no.3
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• pp.168-176
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• 2001

Five different types of catalases from photosynthetic bacterium Rhodospirillum rubrum S1 grown aerobically in the dark were found in this study, and designated Catl (350 kDa), Cat2 (323 kDa), Cat3 (266 kDa), Cat4 (246 kDa), and Cat5 (238 kDa). Analysis of native PAGE revealed that Cat2, Cat3, and Cat4 were also produced in the cells anaerobically grown in the light. It is notable that only Cat2 was expressed much more strongly in response to the anaerobic condition. Enzyme activity staining demonstrated that Cat3 and Cat4 had bifunctional catalase-peroxidase activities, while Catl, Cat2, and Cat5 were typical monofunctional catalases. S1 cells grown aerobically in the presence of malate as the sole source of carbon exhibited an apparent catalase Km value of 10 mM and a Vmax of about 705 U/mg protein at late stationary growth phase. The catalase activity of Sl cells grown in the anaerobic environment exhibited a much lower Vmax of about 109 U/mg protein at late logarithmic growth phase. The catalytic activity was stable in the broad range of temperatures (30$\^{C}$-60$\^{C}$), and pH (6.0-10.0). R. rubrum S1 was much more resistant to H$_2$O$_2$in the stationary growth phase than in the exponential growth phase regardless of growth conditions. Cells of stationary growth phase treated with 15 mM H$_2$O$_2$for 1 h showed 3-fold higher catalase activities than the untreated cells. In addition, L-glutamate induced an 80-fold increase in total catalase activity of R. rubrum S1 compared with magic acid. Through fraction analyses of S1 cells, Cat2, Cat3, Cat4 and Cat5 were found in both cytoplasm and periplasm, while Catl was localized only in the cytoplasm.

• Proceedings of the Korean Information Science Society Conference
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• 2006.06d
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• pp.28-30
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• 2006

스트리밍 서비스는 인터넷 트래픽의 많은 부분을 차지할 정도로 인기 있는 서비스가 되었고, 확장성을 위해 P2P기반의 스트리밍 서비스가 제안되었다. P2P기반 스트리밍 환경은 빈번한 피어들의 떠남과 합류가 일어난다. 이러한 멀티캐스트 그룹의 변화에 대처하기 위해서 다중 멀티캐스트 트리가 제안되었다. 이는 중복성을 통해 멀티캐스트 그룹의 변화에 따른 영향을 줄였다. 하지만 노드의 능력 차이를 고려하지 않았기 때문에 트리가 길어지고, 불안정해질 수 있다. 이를 위해 본 논문은 노드의 능력을 고려한 내구적 멀티캐스트 트리 생성 기법(R-CAT)을 제시하여 우수 노드를 트리의 상층부에 위치시킴으로써 트리의 길이를 줄이고 트리 상층부의 안정화 문제를 해결할 수 있다. 또한 제시한 기법의 유효성을 증명하기 위해 기존의 SplitStream을 확장해서 R-CAT을 구현, 비교 검증한다.

• Proceedings of the Korea Information Processing Society Conference
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• 2005.05a
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• pp.989-992
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• 2005

CAT(Computer Adaptive Testing : 컴퓨터 기반 적응적 검사)는 기존의 종이 시험지에서 이루어지던 시험과 달리 수험자에게 적절한 맞춤식 출제로 보다 정확한 수험자의 능력 판단 및 빠른 수험진행을 가능케 하였다. 기존의 CAT는 많은 인원과 문제가 있어야만 그 결과에 신뢰성이 있다고 알려져 있다. CAT의 대표적인 알고리즘인 SPRT와 EXSPRT-R을 이용하여 10명의 적은 인원으로 JLPT 4급 기출문제를 적용한 실험을 하였다. SPRT 에서는 인원수와, 문제 난이도를 무시한 결과로 인하여 만족 할만한 결과를 얻지 못하였으나, EXSPRT-R의 경우에는 적은 인원에서도 충분히 CAT를 이용할 수 있음을 발견할 수 있었다.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.239-239
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• 2002

• The KIPS Transactions:PartA
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• v.13A no.2 s.99
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• pp.147-156
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• 2006

Recently, streaming service accounts for large part of internet traffic and it is becoming the most popular service. Because of P2P's scalability, P2P-based streaming system is proposed. There are frequent leave and join of a node. To overcome the group dynamics, Multiple Multicast Trees Methods were suggested. However, since they did not consider discrepancy in peers' capacity, it may cause the trees to be long and unstable. So we suggest Resilient Capacity-Aware Multicast Tree construction scheme (R-CAT) that promotes superior peer to upper position in the tree and construct more stable and short multicast trees. By simulation we can show that R-CAT cost more overhead packets for tree joining process, but it reduce the end-to-end delay of the resulting tree and the number of packets lost during the node joining and leaving processes much more than SplitStream.

• Korean Journal of Microbiology
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• v.44 no.4
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• pp.271-276
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• 2008

Ribosome stalling by nascent sticky peptide has been reported in several organisms across the kingdom. To test whether small subunit (SSU) rRNA is involved in this phenomenon, we developed a genetic system that utilized the specialized ribosome system to isolate SSU rRNA mutants that enable ribosomes to bypass the SecM-derived sticky peptide in protein synthesis. In this system, CAT-SecM mRNA, which encodes CAT protein containing the sticky peptide derived from SecM, is only translated by specialized ribosomes. These ribosomes were shown to transiently stall on CAT-SecM mRNA followed by the synthesis of the sticky peptide. Expression of specialized ribosomes resulted in the decreased steady-state level of CAT-SecM mRNA, which is consistent with a notion that ribosome stalling induces mRNA degradation. Isolation and characterization of SSU rRNA mutations using this genetic system that are sufficient to circumvent ribosome stalling induced by the SecM-derived sticky peptide will provide evidence of SSU rRNA function in mRNA cleavage.