• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.128-128
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• 1995

To investigate the mechanism of the regulation of cytochrome P450IA1 gene expression, ethoxyresorufin deethylase(EROD) and benzo(a)pyrene hydroxylase in B6 mouse liver, in isolated perfused rat liver system. and in B6 mouse hepatocyte Hepa-I cells were examined. In C57BL/6N mouse, 3-methylcholan- throne( 3MC ) treatment have resulted in the stimulation of EROD activity based on fluorometry by 2.79 fold comparirng with that of control. Measurement of mRNA of cytochrome P450 was carried out by either nothern blot or dot blot analysis. Findings are similar to that of studies with enzymes. Furhtermore, when RTPCR method was applied to detect mRNA in Hepa I cell and liver tissues the results were more clear. Cytochrome P450IA1 upstream DNA containing CAT construct was transfected into Hepa-1 cells. After transfection of CAT construct, 3MC and flavonoids, such as, chrysin, hesperetin, kaempferol, morin, myricetin and aminoyrine were treated. 48 Hours after treatments, cells were harvested and assayed for CAT mRNA by RTPCR. 3MC treatment to hepa I cells transfected with trout P450IA1-CAT construct increased CAT mRNA by 2.81 fold when it was compared with that of control. This increase CAT mRNA was decreased by concomitantly treated flavonoids and aminopyrine. The level of CAT protein was 29.2-58.0% of 3MC stimulated CAT protein. Results of this study suggested that RTPCR seems to be a very good method to study regulation of gene expression in liver tissue or Hepa cells.

• Journal of Aquaculture
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• v.10 no.1
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• pp.33-37
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• 1997

construct containing reporter gene(pFV4CAT) regulated by carp $\beta$-actin promoter was microinjected into the one-cell stage egg of mud loach (Misgurnus mizolepis), and was successfully expressed, possibly by the integration into the genome. Both mean hatching success and early survival of the microinjected groups were not significantly different with those of control groups (P>0.05). The incidence of transgene was ranged from 7 to 48% based on the PCR and/or Southern blot analyses with the DNA prepared from fin or blood tissue. The spatial and temporal patterns of expression of the pFV4CAT gene, measured by in situ immunohistochemical analysis peroxidase-conjugated anti-CAT antibody, were variable among the experimental individuals. These results suggest that carp $\beta$-actin promoter is effective to express other transgene in mud loach, such that this promoter can be useful in the generation of valuable transgenic mud loach.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.10
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• pp.2171-2179
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• 2009

In this paper, we propose a novel Image Encryption using MLCA (Maximum Length Cellular Automata) and CAT (Cellular Automata Transform). Firstly, we use the Wolfram rule matrix to generate MLCA state transition matrix T. Then the state transition matrix T changes pixel value of original image according to pixel position. Next, we obtain Gateway Values to generate 2D CAT basis function. Lastly, the basis function encrypts the MLCA encrypted image into cellular automata space. The experimental results and security analysis show that the proposed method guarantees better security and non-lossy encryption.

• Exercise Science
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• v.26 no.3
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• pp.204-211
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• 2017

PURPOSE: This study was to investigate the effects of resistance exercise on serum inflammatory markers and CatSper 1-4 expression in testis of OLETF rats. METHODS: Male OLETF rats were divided into two groups; control group (n=12), resistance exercise group (n=12). The exercise group performed a total of 8 weeks of moderate intensity resistance exercise on a 1.35 m vertical ladder with weights secured to their tails. RESULTS: The results of this study was following; The exercise group showed a significant decreased in the inflammatory ($IL-1{\beta}$, IL-6, $TNF-{\alpha}$) levels as compared to the control group. But CRP was no significant difference between control and exercise groups. Also, CatSper 1 and 2 were significantly increased in the exercise group compared to the control group, whereas CatSper 3, 4 were no significant difference between both groups. CONCLUSIONS: This study suggests that resistance exercise training can contribute to reduce pro-inflammatory responses in whole body and it affects male reproductive function by improve sperm quality and CatSper protein expression.

• Journal of Korea Multimedia Society
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• v.24 no.2
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• pp.233-244
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• 2021

Recently, several ICT-based cat studies have produced some successful results, according to academic and industry sources. However, research on the level of simply identifying the cat's condition, such as the behavior and sound classification of cats based on images and sound signals, has yet to be found. In this paper, based on the veterinary scientific knowledge of cats, a practical and academic cat monitoring and disease diagnosis system is proposed to monitor the health status of the cat 24 hours a day by automatically categorizing and analyzing the behavior of the cat with location information using LSTM with a beacon sensor and a raspberry pie that can be built at low cost. Validity of the proposed system is verified through experimentation with cats in actual custody (the accuracy of the cat behavior classification and location identification was 96.3% and 92.7% on average, respectively). Furthermore, a rule-based disease analysis system based on the veterinary knowledge was designed and implemented so that owners can check whether or not the cats have diseases at home (or can be used as an auxiliary tool for diagnosis by a pet veterinarian).

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.712-720
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• 2009

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).

• Journal of Microbiology
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• v.44 no.4
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• pp.447-452
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• 2006

The murine ecotropic retroviral receptor has been demonstrated to function as a mouse cationic amino acid transporter 1(mCAT1), and is comprised of multiple membranespanning domains. Feral mouse (Mus dunni) cells are not susceptible to infection by the ecotropic Moloney murine leukemia virus (MoMLV), although they can be infected by other ecotropic murine leukemia viruses, including Friend MLV and Rauscher MLV. The relative inability of MoMLV to replicate in M. dunni cells has been attributed to two amino acids $(V_{214}\;and\;G_{236})$ located within the third extracellular loop of the M. dunni CAT1 receptor (dCAT1). Via the exchange of the third extracellular loop of the mCAT1 cDNA encoding receptor from the permissive mouse and the corresponding portion of cDNA encoding for the nonpermissive M. dunni receptor, we have identified the most critical amino acid residue, which is a glycine located at position 236 within the third extracellular loop of dCAT1. We also attempted to determine the role of the third extracellular loop of the M. dunni CAT1 receptor with regard to the formation of the syncytium. The relationship between dCAT1 and virus-induced syncytia was suggested initially by our previous identification of two MLV isolates (S82F in Moloney and S84A in Friend MLV), both of which are uniquely cytopathic in M. dunni cells. In an attempt to determine the relationship existing between dCAT1 and the virally-induced syncytia, we infected 293-dCAT1 or chimeric dCAT1 cells with the S82F pseudotype virus. The S82F pseudotype virus did not induce the formation of syncytia, but did show increased susceptibility to 293 cells expressing dCATl. The results of our study indicate that S82F-induced syncytium formation may be the result of cell-cell fusion, but not virus-cell fusion.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.91-98
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• 1997

The transgene, pFV4CAT, containing CAT reporter gene regulated by carp $\beta$-actin promoter, was expressed in independent transgenic mud loach germ lines, determined by reverse transcriptase-PCR (RT-PCR) and enzyme-linked immunosorbant assay (ELISA). Expression of the transmitted transgene was found to be tissue-specific in F1 and F2 generations. Tissue specificity of the expression was dependent on each transgenic line with reproducible patterns. Liver and spleen did express the transgene more frequently than other tissues tested, and muscle and heart revealed the higher amount of CAT than other tissues, while testes showed the lowest expression level. The highest level of CAT expression in muscle from a transgenic F1 line was corresponding to 68-fold compared to the basal levels of controls.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.22 no.4
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• pp.246-252
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• 2011

Background : The objective of this study was to evaluate the diagnostic efficiency of the Comprehensive Attention Test (CAT) by comparing the results of the continuous performance test (CPT) and the CAT in children and adolescents with attention-deficit hyperactivity disorder (ADHD). Method : A total of 110 children and adolescents with ADHD (mean age : 11.2${\pm}$2.9 years, 76 boys) and 36 children and adolescents without ADHD (mean age : 12.0${\pm}$2.7 years, 25 boys) completed the CAT. We compared the specificity and sensitivity of the CPT and CAT at two different cut-off points via the McNemar test. The areas under the receiver operating characteristic curves (AUC) between the two groups were compared using Medcalc software. Results : The sensitivity and specificity of the CPT were .419 and .806, respectively. The sensitivity of the CAT was .827, which was significantly higher than that of the CPT (p<.001), and the specificity of CAT is .444, which was significantly lower than that of CPT (p<.001). The AUC of the CPT and CAT was .664 and .692, respectively, and there were no significant difference between the two groups on the paired comparison (p=.513). Conclusions : The results of this study suggest that the CAT has moderate specificity and high sensitivity. The CAT can be used as a useful tool to evaluate the neuropsychological function of children and adolescents with ADHD.

• Journal of Veterinary Clinics
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• v.34 no.1
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• pp.34-38
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• 2017

The aims of the present study were to determine the weaning time for adoption of kittens, and to evaluate the fetal growth rate during pregnant in free-roaming cats. This study was conducted on three pregnant free-roaming cats (one feral cat and two stray cats). Radiography and ultrasonography were performed on the feral cat and on one of the stray cats. In the feral cat, fetal head diameter was measured once during pregnancy to determine the cesarean section (C-sec) time. In the stray cat, serial fetal head diameter was measured from capture to parturition. The body weight of the feral cat's kittens was measured from 4 weeks postpartum because of their wildness. That of the stray cats' kittens was measured immediately after birth. In the feral cat, scheduled C-sec was performed at predicted parturition day by measurement of head diameter, and six healthy kittens were delivered. The stray cats had five and six kittens by natural delivery, respectively. In the body weight gain of feral and stray cat's kittens, two female kittens of the feral cat lost weight rapidly after they were separated from their mother, so they were returned to their mother for 1 more week. After that, the female kittens grew up without difficulty. Body weight gain of the ten kittens born to the two stray cats consistently increased, by approximately 14 g every day, until they were adopted. The body weight of kittens born by natural delivery was on average 77.5 g greater than that of kittens born by C-sec. However, the gap decreased with time. During the stray cat's pregnancy, fetal head diameter increased by 0.042 cm every day. Maximum head size before parturition was 2.43 cm. These results indicate that the weaning time for adoption of kittens was 5-week-old postpartum.