• Title/Summary/Keyword: Castasterone

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Ability for Biosynthesis of C27 Brassinosteroids by an Enzyme Pool Prepared from Cultured Cells of Phaseolus vulgaris (강낭콩 현탁배양세포에서 추출된 Enzyme Pool의 C27 Brassinosteroids 합성 능력)

  • Kim Tae-Wuk;Joo Se-Hwan;Kim Seong-Ki
    • Journal of Plant Biotechnology
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    • v.32 no.1
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    • pp.63-71
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    • 2005

  • A cell-free enzyme solution prepared from suspension cultured cells of Phaseolus vulgaris successfully mediated conversions of cholesterol $\to$ cholestanol and 6-deoxo-28-norteasterone $\leftrightarrow$ 6-deoxo-28-nor-3-dehydroteasterone $\leftrightarrow$ 6-deoxo-28-nortyphasterol $\to$ 6-deoxo-28-norcastasterone $\to$ 28-norcastasterone. Al-though conversion of cholestanol to 6-deoxo-28-norteasterone intermediated by 6-deoxo-28-norcathasterone was not demonstrated, this strongly suggests that a complete set of biosynthetic enzymes catalyzing reactions from cholesterol to 28-norcastasterone via 6-deoxo-28-nor type brassinosteroids is endogenously present in the cells, which demonstrates that a $C_{27}$ brassinosteroids biosynthetic pathway, namely the late C-6 oxidation for $C_{27}$ brassinosteroids, is operative in the cells. Additionally, the enzyme solution mediated conversion of 28-norcastasterone to castasterone in the presence of S-adenosyl-methionine and NADPH, providing that the $C_{27}$ brassinosteroids biosynthesis is an important route to generate castasterone in the cells. Together with our previous finding that castasterone can be biosynthesized by the same biosynthetic pathway in tomato, this study demonstrates that the $C_{27}$ brassinosteroids biosynthesis is a common alternative process to maintain endo-genous level of castasterone, an active $C_{28}$ brassinosteroid, in plants.

  • https://doi.org/10.5010/JPB.2005.32.1.063 인용 PDF KSCI

Occurrence of Castasterone, Brassinolide and Methyl 4-Chloroindole-3-acetate in Immature Vicia faba Seeds (잠두(Vicia faba L)가 생산하는 Brassinosteroid 활성물질)

  • Park, Keun-Hyung
    • Applied Biological Chemistry
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    • v.31 no.1
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    • pp.106-113
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    • 1988

  • The rice lamina inclination test indicated the presence of brassinosteroid-like active substances in immature Vicia faba seeds. Two of these were identified as castasterone and brassinolide by GC/MS and GC/SIM, respectively. Another active principle was identified as methyl 4-chloroindole-3-acetate by GC/MS and HPLC.

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Brassinosteroid substances in immature Cassia tora seeds (결명자의 brassinosteroid 활성물질)

  • Park, Keun-Hyung;Kim, Seon-Jae;Hyun, Kyu-Hawn
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.99-104
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    • 1993

  • In order to explore the brassinosteroid-active component in Cassia tora, methanol extract of immature seeds was purified by sequences of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography and Bondesil chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography. Brassinosteroid substances in separated active fractions were identified as teasterone, castasterone, brassinolide by TLC and HPLC. Our work is probably the first report of endogenous brassinosteroid in Cassia tora. The content of brassinosteroid in Cassia tora as converted into brassinolide was $3.5{\sim}5.5\;ng/g$ fresh weight. The order of brassinosteroid contents was toward to be teasterone, castasterone, brassinolide.

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Brassinosteroids in Rice Bran and Polished Rice (미강과 백미의 Brassinosteroid 활성물질)

  • Park, Kyoung-Won;Park, Jong-Dae;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.28 no.2
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    • pp.360-365
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    • 1996

  • To investigate the presence of brassinosteroids in rice bran and pㅐlished rice, they were extracted with MeOH. The extracts were purified through sequential procedure of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography and charcoal adsorption chromatography. The activity of brassinosteroids was monitored by the rice inclination test and its presence was confirmed in each purification step. The purified active components were further separated by TLC and HPLC. Brassinosteroids in active fractions of rice bran were identified as castasterone and teasterone by HPLC. We acknowledge that our work is probably the first report of brassinosteroids in mature seeds of rice The more amount of brassinosteroids was confirmed in rice bran than polished rice. The contents of castasterone and teasterone which were identified in rice bran were 0.15 ng/g and 0.37 ng/g, respectively.

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Identification of Brassinosteroids in Immature seeds of Zea mays by GC-MS analysis (GC-MS에 의한 옥수수 미숙종자의 Brassinosteroid 동정)

  • Kim, Seon-Jae;Yokota, T.;Park, Keun-Hyung
    • Applied Biological Chemistry
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    • v.38 no.2
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    • pp.179-183
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    • 1995

  • The brassinosteroid-like active principles from immature seeds of Zea mays were purified and identified as castasterone, teasterone, 28-norcastasterone and 6-deoxocastasterone by GC-MS analysis. Our work is probably the first report that showed the presence of brassinosteroids in Zea mays seeds.

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