• Journal of Information Management
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• v.35 no.2
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• pp.41-55
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• 2004

This study is expected to discuss some methods regarding the uplift of user's use convenience and the efficiency of material management by constructing the multimedia database through the digitalization of the especial audiotape-typed materials among accompanying materials. In order to do that, this paper dealt with the present management conditions of accompanying materials, the sorts of audio data formats, data format transformation, the methods of administration and utilization, etc. And this paper also presented the expected effect and problems caused by multimedia databases construction of accompanying materials.

• Molecules and Cells
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• v.38 no.11
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• Journal of Microbiology
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• v.45 no.5
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• pp.379-387
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• 2007

A hundred and seventeen antibiotic-resistant Escherichia coli strains were isolated from public tap and spring waters which were polluted by fecal coliforms. There were no significant differences between two water sources as to the coliform pollution level (p> 0.05). All E. coli isolates were detected to be resistant to one or more antibiotics tested. Nearly 42% of the isolates showed multiresistant phenotype. Three (2.5%) of these isolates contained class 1 integron. Sequencing analysis of variable regions of the class 1 integrons showed two gene cassette arrays, dfr1-aadA1 and dhfrA17-aadA5. Resistance to ampicillin, tetracycline or trimethoprim-sulfamethoxazole was transferable according to the results of conjugation experiments. The rate of tetracycline resistance was 15%. tet(A)-mediated tetracycline resistance was widespread among tetracycline-resistant E. coli isolates. Genotyping by BOX-polymerase chain reaction (BOX-PCR) showed that some of the strains were epidemiologically related. This is the first report on the prevalence and characterization of class 1 integron-containing E. coli isolates of environmental origin in Turkey.

• Proceedings of the Microbiological Society of Korea Conference
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• 2000.05a
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• pp.58-65
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• 2000

Diarrheal diseases are a major cause of both illness and death in developing countries and are caused by rotavirus, Shigella spp., Salmonella spp., enterotoxigenic Escherichia coli (ETEC), and Vibrio spp. In this study, for the development of vaccine against diarrheal diseases caused by Shigella sonei, Salmonella typhimurium, E. coli O157, and Vibrio cholerae, cloning and nucleotide sequence analysis of genes and characteristics of their gene products in E. coli were performed. For construction of attenuated strain of S. sonnei KNIH104 and Salmonella typhimurium KNIH100, the aroA genes were cloned, respectively. The recombinant plasmid $_pJP{\Delta}A45$ containing aroA deleted region and suicide vector $(_pJP5603)$ was constructed. The aroA gene deleted mutants were constructed using this recombinant plasmid. For cloning gene encoding antigenic region of E. coli O157 KNIH317, the O-antigen synthesis gene cluster and sit gene was cloned. The E. coli XL1-Blue cells harboring this recombinant plasmid showed cytotoxicity in Vero cells. The ctx gene was cloned for tile purpose of antigenic region against V. cholerae KNIH002. Sequence analysis confirmed that the virulence gene cassette was consisted of ace, zot, ctxA and ctxB genes.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1983-1992
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• 2016

Pseudomonas strains isolated from the heavily contaminated Lubin copper mine and Zelazny Most post-flotation waste reservoir in Poland were screened for the presence of integrons. This analysis revealed that two strains carried homologous DNA regions composed of a gene encoding a DNA_BRE_C domain-containing tyrosine recombinase (with no significant sequence similarity to other integrases of integrons) plus a three-component array of putative integron gene cassettes. The predicted gene cassettes encode three putative polypeptides with homology to (i) transmembrane proteins, (ii) GCN5 family acetyltransferases, and (iii) hypothetical proteins of unknown function (homologous proteins are encoded by the gene cassettes of several class 1 integrons). Comparative sequence analyses identified three structural variants of these novel integron-like elements within the sequenced bacterial genomes. Analysis of their distribution revealed that they are found exclusively in strains of the genus Pseudomonas.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.29 no.2
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• pp.74-81
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• 2017

In this study, the combined-type diffuser developed by the Authors, in a previous study, was applied to a residential space. The performance of a ventilation and thermal environment, created by the use of a combined-type diffuser was compared to the pan-type diffuser widely used in apartment houses. In cooling conditions, because of the relatively high air flow rate of ceiling cassette-type air conditioners, the characteristics of airflow distribution in a room were governed by the air conditioner's airflow. In heating conditions, because of the low air flow rate of the diffuser, the characteristics of airflow distribution were governed by the buoyancy effect created by cold external walls and a hot floor. In terms of the Air Diffusion Performance Index (ADPI), which is a thermal environmental index, the result of a combined-type diffuser was greater than a pan-type diffuser in both of cooling and heating conditions. Consequently, the combined-type diffuser showed equal or superior ventilation and thermal environment performance compared to a pan-type diffuser.

• Proceedings of the ESK Conference
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• 1997.10a
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• pp.179-185
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• 1997

작업장 뿐만 아니라 일상생활에서도 결코 무시될 수 없는 것이 레져활동으로 인한 소음이다. 최근 급격히 늘어가는 개인 휴대용 카세트(Personal Cassette Players: PCP)사용은 심각한 소음성난청 발생이 우려된다. 본 연구는 PCP사용에 관련된 여러 인자들이 청력손실과 어떻게 얼마나 관련이 있는가를 파악 하고 PCP를 일정시간 사용했을 때 오는 청력 손실을 정량적으로 파악, 분석하는데 근본 목적을 둔다. 정상 청력을 가진 피실험자 18명의 대학생들을 대상으로 정상 청취조건 그리고 배경소음(지하철)에 노출 중과 후의 사용환경과 소음원으로 Hard Music, Soft Music 및 Language/News를 고려하여 평균소음도와 최고 소음도를 측정하였다. 또한 현실적 상황을 고려한 2시간 정도의 PCP의 청취 전과 후의 청력변화를 측정하였다. 통계 분석 결과, 사용환경과 소음원에 대해 휴대용 카세트 청취 소음수준이 유의한 차이가 있었으며, 특히 Hard Music 청취시에는 95dB, 그리고 지하철 소음하에서는 92dB의 높은 (위험한) 청취수 준을 보였다. 최고소음수준의 경우 NIOSH기준을 초과하는 117dB 정도의 매우 위험한 수준까지 나타났다. 또한 2 시간 정도의 PCP소음에 노출 전 후의 청력손실이 주파수 대역에 걸쳐 유의하게 나타났으며, 특히, 가장 민감한 주파수 대역인 4000Hz에서 최고 7dB 정도의 청력 손실이 발생하였다. 본 연구 결과에 따라, PCP사용에 대한 청력보호대책안을 제시하고자 한다.

• Journal of the Korea Society of Computer and Information
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• v.21 no.12
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• pp.139-145
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• 2016

In this paper, we propose to evaluate the effect of Resistin-like molecule alpha (Retnla) on the expression of transporters involved in modulating concentrations of peripheral cholesterol and plasma high-density lipoprotein (HDL) cholesterol. High levels of blood cholesterol are a well-recognized risk factor for atherosclerosis and are eliminated via the process of reverse cholesterol transport (RCT). We recently showed that Retnla ameliorates hypercholesterolemia and atherosclerosis by increasing biliary cholesterol secretion, the final step of the process, in low-density lipoprotein receptor-deficient mice. However, the role of Retnla in HDL-mediated cholesterol efflux, initial step of RCT pathway, is not yet clear. To identify cholesterol transport genes regulated by Retnla, we performed an extensive microarray-based gene expression screen using livers from Retnla-overexpressing (Tg) mice and control animals. The most significant change in Retnla-Tg mice was an upregulation of ATP-binding cassette sub-family G member 4 (Abcg4) transport and was validated using quantitative RT-PCR. The validated gene was also induced by treatment of purified Retnla protein in RAW 264.7 cells incubated with acetylated low-density lipoprotein and Hepa1c1c7 cells. Taken together, these results indicates that Retnla might also accelerate initial step of RCT pathway, suggesting therapeutic value of Retnla in the treatment of hypercholesterolemia and atherosclerosis.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.11
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• pp.304-306
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• 1996

Endoscopy has become a crucial diagnostic and theraputic procedure in clinical areas. Over the past three years, we have developed a computerized system to record and store clinical data pertaining to endoscopic surgery of laparascopic cholesystectomy, peviscopic endometriosis, and surgical arthroscopy. In this study, we are developed computer system, which is composed of frame grabber, sound board, VCR control board, LAN card and EDMS(endoscopic data management software). Also, computer system has controled over peripheral instruments as a color video printer, video cassette recorder, and endoscopic input/output signals(image and doctor's speech). Also, we are developed one body system of camels control unit including an endoscopic miniature camera and light source. Our system offer unsurpassed image quality in terms of resolution and color fidelity. Digital endoscopic data management system is based on open architecture and a set of widely available industry standards, namely: windows 3.1 as a operating system, TCP/IP as a network protocol and a time sequence based database that handles both an image and drctor's speech synchronized with endoscopic image.

• Journal of the Korean Child Neurology Society
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• v.24 no.3
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• pp.71-83
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• 2016

X-linked adrenoleukodystrophy (X-ALD) is caused by mutations in the ATP binding cassette subfamily D member 1 (ABCD1), a gene that encodes peroxisomal membrane located on ABC half-transporter named adrenoleukodystrophy protein (ALDP). X-ALD is characterized by a highly variable clinical spectrum, including progressive cerebral type, adrenomyeloneuropathy, and addison-only phenotype. No genotype/phenotype correlation has been established. Thus, unidentified modifier genes and other co-factors are speculated to modulate the phenotypic variation and disease severity. Recent advanced sequencing methods and reprogramming technologies not only offer an affordable and applicable approach to investigate the pathophysiological mechanisms of adrenoleukodystrophy, but also provide means to develop therapy. A causal therapy of X-ALD is lacking. Lorenzo's oil therapy is recommended for asymptomatic boys, but the longest study found that the oil was not beneficial at all to symptomatic X-ALD patients. Hematopoietic stem cell therapy has a relevant chance of success when performed during this early stage of cerebral type X-ALD. Recently, it has been insisted that lentiviral-mediated gene therapy of hematopoietic stem cells can provide clinical benefits in X-ALD. This review describes current knowledge on the clinical presentation, pathogenesis, diagnosis and management of X- ALD.