• Journal of Microbiology
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• v.45 no.6
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• pp.492-498
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• 2007

GacS and GacA proteins form a two component signal transduction system in bacteria. Here, Tn5 transposon gacS and gacA (Gac) mutants of Pseudomonas sp. KL28, an alkylphenol degrader, were isolated by selecting for smooth colonies of strain KL28. The mutants exhibited reduced ability to migrate on a solid surface. This surface motility does not require the action of flagella unlike the well-studied swarming motility of other Pseudomonas sp. The Gac mutants also showed reduced levels of biofilm and pellicle formation in liquid culture. In addition, compared to the wild type KL28 strain, these mutants were more resistant to high concentrations of m-cresol but were more sensitive to $H_2O_2$, which are characteristics that they share with an rpoS mutant. These results indicate that the Gac regulatory cascade in strain KL28 positively controls wrinkling morphology, biofilm formation, surface translocation and $H_2O_2$ resistance, which are important traits for its capacity to survive in particular niches.

• Journal of Dairy Science and Biotechnology
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• v.38 no.3
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• pp.134-141
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• 2020

Oxidative stress is a cascade reaction characterized by a significant increase in the amount of oxidized components. Free radicals produced by oxidative stress are one of the common features in several experimental models of disease, and contribute to wide range of neurodegenerative diseases, including Alzheimer's disease. Iron (II) species can participate in the Fenton, and Fenton-like reactions, to react with hydrogen peroxide and generate hydroxyl radical. As iron accumulation and oxidative stress are associated with the pathological progression of neurodegenerative diseases, iron chelation and antioxidant therapies have become strategies to combat these diseases. Due to the complexity of the redox system in vivo, a multifaceted approach may be an attractive therapeutic strategy. Further investigations are highly expected for the prevention and treatment of neurodegenerative diseases in future.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.12
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• pp.1655-1666
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• 2003

It is experimentally well-known that high anisotropies of the turbulent flow field are dominant inside the tip leakage vortex, which is attributable to a substantial proportion of the total loss and constitutes one of the dominant mechanisms of the noise generation. This anisotropic nature of turbulence invalidates the use of the conventional isotropic eddy viscosity turbulence models based on the Boussinesq assumption. In this study, to check whether an anisotropic turbulence model is superior to the isotropic ones or not, the results obtained from the steady-state Reynolds averaged Navier-Stokes simulations based on the RNG k-$\varepsilon$ model and the Reynolds stress model (RSM) are compared with experimental data for two test cases: a linear compressor cascade and a forward-swept axial-flow fan. Through this comparative study of turbulence models, it is clearly shown that the RSM, which can express the production term and body-force term induced by system rotation without introducing any modeling, should be used to predict quantitatively the complex tip leakage flow, especially in the rotating environment.

• International Journal of Fluid Machinery and Systems
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• v.3 no.2
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• pp.122-128
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• 2010

The attachment of inducer in front of main impeller is a powerful method to improve cavitation performance; however, cavitation surge oscillation with low frequency occurs with blade cavity growing to each throat section of blade passage simultaneously. Then, one conceptual method of installing suction axi-asymmetrical plate has been proposed so as to keep every throat passage away from being unstable at once, and the effect on suppression of the oscillation were investigated. In the present study, cavitation behaviors in the inducer is observed with distributing multi-cameras circumferentially, recording simultaneously and reconstructing multi-photos on one plane field as moving a linear cascade. Observed results are utilized for discussion with other measuring results as casing wall pressure distribution. Then the suppression mechanism of oscillation by installing axi-asymmetrical inlet plate will be clarified in more details.

• Proceedings of the IEEK Conference
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• 2001.09a
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• pp.777-783
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• 2001

This paper is mainly concerned with the state of the practical developments of a constants PWM bridge type resonants DC-DC suitable converter for Nd:YAG Laser with a Microprocessor. (PIC16C54 & 8051) The use of IGBT power supply with feedback control of flashLamp currents imparts a advantages to Nd:YAG Laser for materials processing. these include the alility to tailor the pulseshape and modify pulse parameters on a pulse- by pulse basis. And Correct choice of pulseshape can enhance the repeatability of the process. as higher power IGBT became available, act ive pulseforming power supplies will find greater user in deep hole drilling machine By Using certain control tecniques, utililized in designing Pic16c54 from Microchip technology and Intel 8051, also Mornitoring from Microsoft Visual Basic 5, And it allowed us to designed and fabricate ahigh repel it ion rate and high power(HRHP) pulsed Nd:YAG laser system, As a result of that, the current pulsewidth could be contort led 200s to 350s(step 50s) , and the pulse repetition rate could be adjusted 500pps to 1150pps. In addition, in the case of one laser head consisting of a Nd:YAG laser rod and two flashlamps , the maximum laser output of 240w was produced at the condition of 350s and 1150pps, and that of about 480w was generated at the same condition when two laser heads were arranged in cascade.

• Journal of electromagnetic engineering and science
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• v.4 no.4
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• pp.156-161
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• 2004

The MMIC cascode VCO is designed, fabricated, and measured for Ku-band Low Noise Blcok(LNB) system using InGaP/GaAs HBT technology. The phase noise of -116.4 dBc/Hz at 1 MHz offset with output power of 1.3 dBm is obtained at 11.526 GHz by applying 3 V and 11 mA, which is comparatively better characteristics than compared with the different configuration VCOs fabricated with other technologies. The simulated results of oscillation frequency and second harmonic suppression agree with the measured results. The phase noise is improved due to the use of the smallest value of inductor in frequency determining network and the InGaP ledge function of the technology. The chip size of $830\time781\;{\mu}m^2$ is also achieved.

• BMB Reports
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• v.32 no.3
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• pp.215-225
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• 1999

Phytochromes (with gene family members phyA, B, C, D, and E) are a wavelength-dependent light sensor or switch for gene regulation that underscore a number of photo responsive developmental and morphogenic processes in plants. Recently, phytochrome-like pigment proteins have also been discovered in prokaryotes, possibly functioning as an auto-phosphorylating/phosphate-relaying two-component signaling system (Yeh et al., 1997). Phytochromes are photochromically convertible between the light sensing Pr and regulatory active Pfr forms. Red light converts Pr to Pfr, the latter having a "switch-on" conformation. The Pfr form triggers signal transduction pathways to the downstream responses including the expression of photosynthetic and other growth-regulating genes. The components involved in and the molecular mechanisms of the light signal transduction pathways are largely unknown, although G-proteins, protein kinases, and secondary messengers such as $Ca^{2+}$ ions and cGMP are implicated. The 124-127 kDa phytochromes form homodimeric structures. The N-terminal half contains the tetrapyrrolic phytochromobilin for red/far-red light absorption. The C-terminal half includes both a dimerization motif and regulatory box where the red light signal perceived by the chromophore-domain is recognized and transduced to initiate the signal transduction cascade. A working model for the inter-domain signal communication within the phytochrome molecule is proposed in this Review.

• BMB Reports
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• v.28 no.4
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• pp.316-322
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• 1995

The GTPase activating protein (GAP) can function both as a negative regulator and an effector of $p21^{ras}$. Overexpression of GAP in NIH-3T3 cells has been shown to inhibit transformation by ms or src. To investigate the function of GAP in a differentiative system, we overexpressed this protein in the nerve growth factor (NGF)-responsive PC12 cell line. Two-fold overexpression of GAP caused a delay of several days in the onset of NGF- but not FGF-induced neuronal differentiation of PC12 cells. However, the NGF-induced activation or tyrosine phosphorylation of upstream (Trk, PLC-${\gamma}1$, SHC) and downstream (B-Raf and $p44^{mapk/erk1}$) components of $p21^{ras}$, signalling cascade was not altered by GAP overexpression. Therefore, the change of phenotype induced by GAP was probably not due to GAP functioning as a negative regulator of $p21^{ras}$. Rather, we found that NGF-induced tyrosine phosphorylation of SNT, a specific target of neurotrophin-induced tyrosine kinase activity, was inhibited by GAP overexpression. SNT is thought to function upstream or independent of $p21^{ras}$. Thus in PC12 cells, overexpressed GAP may control the rate of neuronal differentiation through a pathway involving SNT rather than the $p21^{ras}$ signalling pathway.

• Journal of Korean Society for Atmospheric Environment
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• v.13 no.4
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• pp.285-296
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• 1997

The suspended particulate matters had been collected on quartz fiber fiters by a cascade impactor having 9 size stages for 4 years (Sep. 1991 to Dec. 1995) in Kyung Hee University-Suwon Campus. Membrane filters were used to collected the particulate matters on each stage. The weight concentration on each stage was obtained by a microbalance and further chemical element levels were determined by an x-ray fluorescence system. Based on these chemical information, our study focused on applying the target transformation factor analysis (TTFA), a receptor model, to identify aerosol sources and to apportion quantitatively their mass contribution. There are total of 63 ambient data sets. Each data set consists of the 8 size-ranged subdata sets characterized by 16 elemental variables. By the results, four to five sources were extracted from each size range and some sources reappeared in other size ranges. Then total of 8 source profiles were statistically generated from all the ranges, such as oil burning source, soil source, field burning source, gasoline related source, coal burning source, marine source, glass related source, and unknown sources. Apportioning aerosol mass to each source was intensively examined by investigating emission inventories near the study area. The results showed that soil particle source was the most significant contributor. However, coal and oil burning sources were the major anthropogenic ones. The study finally proposed some air quality control strategies to achieve the clean air quality in Suwon area.

• Journal of Integrative Natural Science
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• v.6 no.3
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• pp.125-137
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• 2013

Promising evidence suggests that amyloid beta peptide ($A{\beta}$), a key mediator in age-dependent neuronal and cerebrovascular degeneration, activates death signalling processes leading to neuronal as well as non-neuronal cell death in the central nervous system. A major cellular event in $A{\beta}$-induced apoptosis of non-neuronal cells, including cerebral endothelial cells, astrocytes and oligodendrocytes, is mitochondrial dysfunction. The apoptosis signalling cascade upstream of mitochondria entails $A{\beta}$ activation of neutral sphingomyelinase, resulting in the release of ceramide from membrane sphingomyelin. Ceramide then activates protein phosphatase 2A (PP2A), a member in the ceramide-activated protein phosphatase (CAPP) family. PP2A dephosphorylation of Akt and FKHRL1 plays a pivotal role in $A{\beta}$-induced Bad translocation to mitochondria and transactivation of Bim. Bad and Bim are pro-apoptotic proteins that cause mitochondrial dysfunction characterized by excessive ROS formation, mitochondrial DNA (mtDNA) damage, and release of mitochondrial apoptotic proteins including cytochrome c, apoptosis inducing factor (AIF), endonuclease G and Smac. The cellular events activated by $A{\beta}$ to induce death of non-neuronal cells are complex. Understanding these apoptosis signalling processes will aid in the development of more effective strategies to slow down age-dependent cerebrovascular degeneration caused by progressive cerebrovascular $A{\beta}$ deposition.